pycom02g09910

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000393
Physical Location & Seq
Forward (+)
6850824 .. 6853348
2525 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09910.2

Sequence Viewer

Length: 1011 bp
ATGCGAGTTATACAGCAGACGCGTTTCCACATACTTACACAAATGCTTAAAAACACCTATGAAAACTTAAACATGCAGTGTATATTTTCACCCACCAAGGCAATGAACTTCCTCTCCCCAAAACTTCTTCCTTCATCATCTAAATCTTCTCTCGAACTTATAACAATCACCATAGTCTTATTCTTGTACCGCAATGCAGCTGCTGTAGACCTACCAAACAACGAAAAGATTCCGGCAGTGATTGTTTTTGGAGATTCAATAGTGGATCCGGGAAACAACAACAATATTGGCACGATAGTCAAATGCAATTTCCCACCTTATGGGAGGGACTTTGTAGGAAAAAGGCCTACTGGAAGGTTTAGCAATGGCAGAGTCCCCTCAGACTTGATAGCTGAATCAGTTGGAGTGAAGAAGATTTTGCCAGCTTATTTGGACTCAAATCTGAAGATTCAAGACCTACTTACTGGTGTAAGTTTTGCCTCAGGTGGCTCAGGATATGATCCCCTCACTCCCAAAATCGTGATCGATCTCATGGTTAAATCGGCTTCAAGTTTCTTTCAGGAACTATATGGACTCGGAGCAAGAAGAATTGGAGTTGTAAGTCTGCCAGCCATCGGGTGTGTACCGTCACTGAGAACACTGAGTGGAGGCATAGACAGAGGGTGCTCGGAGACTGCCAACCAAGCAGCAAGTCTCTTCAACTCCAAGCTCTCTGCCCAAATAGATGCCTTCAATAGGAGGCTGCCAGAAGCAAGGCTTGTCTACCTCGATATCTACAACACATTGCTTTCCCTCATCCAAAACCCTACTCAATATGGATTTGAAGTGATCAATCGAGGATGCTGTGGGACGGGAAACGTTGAGGTTAGCATTCTGTGTACACGTTACTCTCCGGGAACCTGCAATTATTCGTCGAAATACATATTCTGGGACAGCTACCATCCTTCAGAGAAAGGATATGAAGTCATCACGCCTCTGGCTTTCGATTCGCAAGTTCATAAGTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

337

Amino Acids

36.94

Weight (kDa)

8.93

Isoelectric Point (pI)

31.11

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 161
Acc36I ACCTGC 1 cut(s) 908
AccB7I CCANNNNNTGG 1 cut(s) 320
AccI GTMKAC 2 cut(s) 207, 762
AccII CGCG 1 cut(s) 22
AciI CCGC 1 cut(s) 190
AclI AACGTT 1 cut(s) 858
AclWI GGATC 3 cut(s) 260, 273, 494
AcuI CTGAAG 2 cut(s) 464, 930
AdeI CACNNNGTG 1 cut(s) 644
AfaI GTAC 3 cut(s) 188, 624, 880
AfiI CCNNNNNNNGG 3 cut(s) 320, 614, 735
AflIII ACRYGT 2 cut(s) 20, 881
AgsI TTSAA 6 cut(s) 258, 452, 549, 700, 733, 824
AloI GAACNNNNNNTCC 2 cut(s) 98, 130
AluBI AGCT 5 cut(s) 200, 392, 425, 709, 936
AluI AGCT 5 cut(s) 200, 392, 425, 709, 936
Alw21I GWGCWC 1 cut(s) 668
Alw26I GTCTC 2 cut(s) 665, 698
AlwI GGATC 3 cut(s) 260, 273, 494
AlwNI CAGNNNCTG 1 cut(s) 203
AoxI GGCC 1 cut(s) 344
ApeKI GCWGC 4 cut(s) 197, 200, 686, 742
Asp700I GAANNNNTTC 1 cut(s) 228
AsuC2I CCSGG 2 cut(s) 270, 894
AsuHPI GGTGA 2 cut(s) 81, 160
AxyI CCTNAGG 1 cut(s) 481
BamHI GGATCC 1 cut(s) 265
Bbv12I GWGCWC 1 cut(s) 668
BbvI GCAGC 4 cut(s) 187, 209, 698, 729
BccI CCATC 2 cut(s) 620, 948
BclI TGATCA 1 cut(s) 828
BcnI CCSGG 2 cut(s) 270, 894
BcoDI GTCTC 2 cut(s) 665, 698
BfmI CTRYAG 1 cut(s) 204
BfuAI ACCTGC 1 cut(s) 908
BisI GCNGC 4 cut(s) 198, 201, 687, 743
BlsI GCNGC 4 cut(s) 199, 202, 688, 744
Bme1390I CCNGG 2 cut(s) 270, 894
BmiI GGNNCC 2 cut(s) 267, 898
BmrFI CCNGG 2 cut(s) 270, 894
BmsI GCATC 2 cut(s) 715, 830
Bpu10I CCTNAGC 1 cut(s) 490
BpuMI CCSGG 2 cut(s) 270, 894
Bsa29I ATCGAT 1 cut(s) 525
BsaJI CCNNGG 1 cut(s) 96
BsaXI ACNNNNNCTCC 2 cut(s) 98, 128
Bsc4I CCNNNNNNNGG 3 cut(s) 320, 614, 735
Bse1I ACTGG 2 cut(s) 355, 469
Bse21I CCTNAGG 1 cut(s) 481
Bse3DI GCAATG 4 cut(s) 108, 199, 370, 782
BseCI ATCGAT 1 cut(s) 525
BseDI CCNNGG 1 cut(s) 96
BseGI GGATG 3 cut(s) 795, 845, 940
BseLI CCNNNNNNNGG 3 cut(s) 320, 614, 735
BseMI GCAATG 4 cut(s) 108, 199, 370, 782
BseMII CTCAG 5 cut(s) 393, 495, 504, 623, 632
BseNI ACTGG 2 cut(s) 355, 469
BseXI GCAGC 4 cut(s) 187, 209, 698, 729
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 1 cut(s) 346
BshVI ATCGAT 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 668
BsiSI CCGG 3 cut(s) 233, 269, 893
BslFI GGGAC 4 cut(s) 341, 359, 862, 944
BslI CCNNNNNNNGG 3 cut(s) 320, 614, 735
BsmAI GTCTC 2 cut(s) 665, 698
BsmFI GGGAC 4 cut(s) 341, 359, 862, 944
BsmI GAATGC 1 cut(s) 870
BsnI GGCC 1 cut(s) 346
Bsp1286I GDGCHC 1 cut(s) 668
Bsp1407I TGTACA 1 cut(s) 878
Bsp143I GATC 5 cut(s) 265, 499, 522, 526, 828
BspACI CCGC 1 cut(s) 190
BspANI GGCC 1 cut(s) 346
BspCNI CTCAG 5 cut(s) 392, 494, 503, 624, 633
BspDI ATCGAT 1 cut(s) 525
BspFNI CGCG 1 cut(s) 22
BspLI GGNNCC 2 cut(s) 267, 898
BspMI ACCTGC 1 cut(s) 908
BspPI GGATC 3 cut(s) 260, 273, 494
BsrDI GCAATG 4 cut(s) 108, 199, 370, 782
BsrGI TGTACA 1 cut(s) 878
BsrI ACTGG 2 cut(s) 355, 469
BssECI CCNNGG 1 cut(s) 96
BssMI GATC 5 cut(s) 265, 499, 522, 526, 828
BssT1I CCWWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 627
Bst6I CTCTTC 1 cut(s) 701
BstAUI TGTACA 1 cut(s) 878
BstC8I GCNNGC 2 cut(s) 423, 609
BstDEI CTNAG 5 cut(s) 379, 481, 490, 632, 641
BstENI CCTNNNNNAGG 1 cut(s) 733
BstF5I GGATG 3 cut(s) 795, 845, 940
BstFNI CGCG 1 cut(s) 22
BstKTI GATC 5 cut(s) 268, 502, 525, 529, 831
BstMAI GTCTC 2 cut(s) 665, 698
BstMBI GATC 5 cut(s) 265, 499, 522, 526, 828
BstMWI GCNNNNNNNGC 1 cut(s) 683
BstNSI RCATGY 1 cut(s) 76
BstSCI CCNGG 2 cut(s) 268, 892
BstSFI CTRYAG 1 cut(s) 204
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 4 cut(s) 187, 209, 698, 729
BstX2I RGATCY 1 cut(s) 265
BstYI RGATCY 1 cut(s) 265
Bsu15I ATCGAT 1 cut(s) 525
Bsu36I CCTNAGG 1 cut(s) 481
BsuRI GGCC 1 cut(s) 346
BsuTUI ATCGAT 1 cut(s) 525
BtsCI GGATG 3 cut(s) 795, 845, 940
BtsI GCAGTG 2 cut(s) 83, 243
BtsIMutI CAGTG 4 cut(s) 83, 243, 629, 638
BveI ACCTGC 1 cut(s) 908
Cac8I GCNNGC 2 cut(s) 423, 609
CaiI CAGNNNCTG 1 cut(s) 203
ClaI ATCGAT 1 cut(s) 525
CseI GACGC 1 cut(s) 28
Csp6I GTAC 3 cut(s) 187, 623, 879
CviAII CATG 2 cut(s) 73, 532
CviQI GTAC 3 cut(s) 187, 623, 879
DdeI CTNAG 5 cut(s) 379, 481, 490, 632, 641
DpnI GATC 5 cut(s) 267, 501, 524, 528, 830
DpnII GATC 5 cut(s) 265, 499, 522, 526, 828
DraIII CACNNNGTG 1 cut(s) 644
Eam1104I CTCTTC 1 cut(s) 701
EarI CTCTTC 1 cut(s) 701
Eco130I CCWWGG 1 cut(s) 96
Eco147I AGGCCT 1 cut(s) 346
Eco32I GATATC 1 cut(s) 772
Eco57I CTGAAG 2 cut(s) 464, 930
Eco81I CCTNAGG 1 cut(s) 481
EcoNI CCTNNNNNAGG 1 cut(s) 733
EcoRV GATATC 1 cut(s) 772
EcoT14I CCWWGG 1 cut(s) 96
ErhI CCWWGG 1 cut(s) 96
FaeI CATG 2 cut(s) 76, 535
FalI AAGNNNNNCTT 4 cut(s) 444, 476, 741, 773
FaqI GGGAC 4 cut(s) 341, 359, 862, 944
FatI CATG 2 cut(s) 72, 531
FbaI TGATCA 1 cut(s) 828
FblI GTMKAC 2 cut(s) 207, 762
Fnu4HI GCNGC 4 cut(s) 198, 201, 687, 743
FokI GGATG 3 cut(s) 782, 852, 927
Fsp4HI GCNGC 4 cut(s) 198, 201, 687, 743
GluI GCNGC 4 cut(s) 198, 201, 687, 743
HaeIII GGCC 1 cut(s) 346
HapII CCGG 3 cut(s) 233, 269, 893
HgaI GACGC 1 cut(s) 28
Hin1II CATG 2 cut(s) 76, 535
HinfI GANTC 8 cut(s) 229, 254, 372, 395, 434, 448, 573, 986
HpaII CCGG 3 cut(s) 233, 269, 893
HphI GGTGA 2 cut(s) 81, 160
Hpy166II GTNNAC 5 cut(s) 208, 623, 763, 879, 881
Hpy188I TCNGA 6 cut(s) 382, 444, 578, 670, 949, 1010
Hpy188III TCNNGA 5 cut(s) 152, 452, 492, 520, 560
Hpy8I GTNNAC 5 cut(s) 208, 623, 763, 879, 881
Hpy99I CGWCG 1 cut(s) 916
HpyAV CCTTC 4 cut(s) 141, 348, 739, 954
HpyCH4III ACNGT 1 cut(s) 627
HpyCH4IV ACGT 2 cut(s) 858, 883
HpyCH4V TGCA 4 cut(s) 76, 197, 306, 903
HpyF10VI GCNNNNNNNGC 1 cut(s) 683
HpyF3I CTNAG 5 cut(s) 379, 481, 490, 632, 641
HpySE526I ACGT 2 cut(s) 858, 883
Hsp92II CATG 2 cut(s) 76, 535
Ksp22I TGATCA 1 cut(s) 828
Kzo9I GATC 5 cut(s) 265, 499, 522, 526, 828
LmnI GCTCC 1 cut(s) 578
Lsp1109I GCAGC 4 cut(s) 187, 209, 698, 729
LweI GCATC 2 cut(s) 715, 830
MaeII ACGT 2 cut(s) 858, 883
MaeIII GTNAC 2 cut(s) 627, 884
MalI GATC 5 cut(s) 267, 501, 524, 528, 830
MboI GATC 5 cut(s) 265, 499, 522, 526, 828
MboII GAAGA 7 cut(s) 119, 138, 421, 424, 457, 597, 688
MflI RGATCY 1 cut(s) 265
MhlI GDGCHC 1 cut(s) 668
MluCI AATT 3 cut(s) 307, 588, 904
MluI ACGCGT 1 cut(s) 20
MlyI GAGTC 3 cut(s) 381, 428, 567
MmeI TCCRAC 1 cut(s) 382
MroXI GAANNNNTTC 1 cut(s) 228
MseI TTAA 3 cut(s) 48, 68, 537
MspA1I CMGCKG 1 cut(s) 200
MspI CCGG 3 cut(s) 233, 269, 893
MspR9I CCNGG 2 cut(s) 270, 894
Mva1269I GAATGC 1 cut(s) 870
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 1 cut(s) 683
NciI CCSGG 2 cut(s) 270, 894
NdeII GATC 5 cut(s) 265, 499, 522, 526, 828
NlaIII CATG 2 cut(s) 76, 535
NlaIV GGNNCC 2 cut(s) 267, 898
NmuCI GTSAC 1 cut(s) 627
NspI RCATGY 1 cut(s) 76
PceI AGGCCT 1 cut(s) 346
PctI GAATGC 1 cut(s) 870
PdmI GAANNNNTTC 1 cut(s) 228
PfeI GAWTC 5 cut(s) 229, 254, 395, 448, 986
PflMI CCANNNNNTGG 1 cut(s) 320
PfoI TCCNGGA 2 cut(s) 268, 892
PkrI GCNGC 4 cut(s) 199, 202, 688, 744
PleI GAGTC 3 cut(s) 380, 428, 567
PpsI GAGTC 3 cut(s) 380, 428, 567
PsiI TTATAA 1 cut(s) 161
Psp1406I AACGTT 1 cut(s) 858
PspN4I GGNNCC 2 cut(s) 267, 898
PstNI CAGNNNCTG 1 cut(s) 203
PsuI RGATCY 1 cut(s) 265
PvuII CAGCTG 1 cut(s) 200
RsaI GTAC 3 cut(s) 188, 624, 880
RsaNI GTAC 3 cut(s) 187, 623, 879
SaqAI TTAA 3 cut(s) 48, 68, 537
SatI GCNGC 4 cut(s) 198, 201, 687, 743
Sau3AI GATC 5 cut(s) 265, 499, 522, 526, 828
SchI GAGTC 3 cut(s) 381, 428, 567
ScrFI CCNGG 2 cut(s) 270, 894
SduI GDGCHC 1 cut(s) 668
SfaNI GCATC 2 cut(s) 715, 830
SfcI CTRYAG 1 cut(s) 204
Sse9I AATT 3 cut(s) 307, 588, 904
SseBI AGGCCT 1 cut(s) 346
SsiI CCGC 1 cut(s) 190
SspI AATATT 1 cut(s) 286
StuI AGGCCT 1 cut(s) 346
StyD4I CCNGG 2 cut(s) 268, 892
StyI CCWWGG 1 cut(s) 96
TaaI ACNGT 1 cut(s) 627
TaiI ACGT 2 cut(s) 861, 886
TaqI TCGA 6 cut(s) 153, 525, 768, 835, 914, 984
TasI AATT 3 cut(s) 307, 588, 904
TatI WGTACW 1 cut(s) 878
TfiI GAWTC 5 cut(s) 229, 254, 395, 448, 986
Tru1I TTAA 3 cut(s) 48, 68, 537
Tru9I TTAA 3 cut(s) 48, 68, 537
TscAI CASTG 4 cut(s) 83, 243, 636, 645
TseFI GTSAC 1 cut(s) 627
TseI GCWGC 4 cut(s) 197, 200, 686, 742
Tsp45I GTSAC 1 cut(s) 627
TspDTI ATGAA 5 cut(s) 75, 119, 123, 975, 986
TspRI CASTG 4 cut(s) 83, 243, 636, 645
Van91I CCANNNNNTGG 1 cut(s) 320
XagI CCTNNNNNAGG 1 cut(s) 733
XceI RCATGY 1 cut(s) 76
XmiI GTMKAC 2 cut(s) 207, 762
XmnI GAANNNNTTC 1 cut(s) 228
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.