Rw2G027630

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000393
Physical Location & Seq
Forward (+)
43615462 .. 43617296
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027630.1

Sequence Viewer

Length: 1089 bp
ATGGCTTTTCTCTCACAAATACTTGTTCCTTCATCTTCATCTGTCGAAATTTTCTCAATCATTGCAGTCATTTTCCTCTCCCATAATGTTGCTGCAGTAACACCAGCCACAAATAAAACAACTAGAGCAGTTTTTACTTTCGGAGATTCAATAATGGACACGGGCAATAATGACTACATCATTTCTATAGCCAAAAGCAATTTCCCACCTTATGGGAGAGACTTTGCGGGAGGAAAGCCTACTGGAAGATTTAGCAATGGAAAAGTCGCCTCAGATTTTGCTGCTGAAATGTATGGAGTGAAGAAGATTTTGCCGGCTTATCTGGATCCGAAATTGCAGCTTCAAGACCTACTTACTGGTGTATGTTTTGCCTCAGCAGGATCAGGATATGACCCTCTCACTGCCAAATTACTTTCTGTCTTGTCACTACCAGATCAATTAGACTTGTTTAAAAAATACAAAAGCAAGATAACTGCAGCAGTTGGGAATGAAAGAACTGCCACTATACTGTCGAAAAGCATTTCCTTTCTTTCCATGGGAAGCAATGACCTTGCAATTGATTATTTTTCTACACCACTAAGGAGCCCTCACTATGATATTCCAGCTTATACAGATCTCATGATCGAATCAGCTTCAAATTTCTTGCAGGAACTTCATGCACTGGGATTAAGAGTGATCGGAGTAATATGTATACCACCAATTGGGTGTTTGCCAGCACAGAGAACAGTTAATGGAGGTATAAAGAGAGCGTGCTACGAAACCGGGAATCAAGCAGCAACCCTTTTCAACTCGAAGCTCTCCCACCTTATAGATTCCCTCAATGAGAGACTTCCAGAAGCACACCTCATGTACCTTGATAGCTACAGCCCTGCGCTTTCCCTGATCCAAAACCCTGCTCAATATGGTTTTGAAGTGGTGGATAAAGGATGCTGTGGAACAGGAGATCTTGAAATTGGCCCTCTGTGTAACAAAGACACCCCGGGTACTTGTAACGATGTGTCCAAGTACATATTCTGGGATAGCTTTCATCCTACAGAGAAGGCATACAGGATCCTCACCTCTATTATTTTCAAAGGAGTTAAGATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

362

Amino Acids

39.25

Weight (kDa)

6.59

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 353 3.5e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 212, 701
AccI GTMKAC 1 cut(s) 691
AciI CCGC 1 cut(s) 227
AclWI GGATC 6 cut(s) 320, 333, 388, 877, 1045, 1058
AcsI RAATTY 2 cut(s) 48, 637
AfaI GTAC 3 cut(s) 851, 985, 1007
AfiI CCNNNNNNNGG 2 cut(s) 212, 701
AgsI TTSAA 7 cut(s) 150, 344, 636, 787, 911, 950, 1072
AjuI GAANNNNNNNTTGG 4 cut(s) 185, 217, 995, 1027
AluBI AGCT 6 cut(s) 340, 605, 632, 796, 861, 1023
AluI AGCT 6 cut(s) 340, 605, 632, 796, 861, 1023
Alw26I GTCTC 2 cut(s) 213, 820
AlwI GGATC 6 cut(s) 320, 333, 388, 877, 1045, 1058
Ama87I CYCGRG 1 cut(s) 979
AoxI GGCC 1 cut(s) 955
ApeKI GCWGC 5 cut(s) 92, 281, 337, 476, 773
ApoI RAATTY 2 cut(s) 48, 637
AspLEI GCGC 1 cut(s) 874
AspS9I GGNCC 1 cut(s) 956
AsuC2I CCSGG 3 cut(s) 763, 980, 981
AsuHPI GGTGA 1 cut(s) 1048
AvaI CYCGRG 1 cut(s) 979
BamHI GGATCC 2 cut(s) 325, 1050
BanII GRGCYC 1 cut(s) 587
BbvCI CCTCAGC 1 cut(s) 373
BbvI GCAGC 5 cut(s) 79, 268, 349, 488, 785
BcnI CCSGG 3 cut(s) 763, 980, 981
BcoDI GTCTC 2 cut(s) 213, 820
BfaI CTAG 1 cut(s) 123
BfmI CTRYAG 5 cut(s) 93, 186, 474, 862, 1032
BglII AGATCT 2 cut(s) 613, 943
BisI GCNGC 5 cut(s) 93, 282, 338, 477, 774
BlsI GCNGC 5 cut(s) 94, 283, 339, 478, 775
Bme1390I CCNGG 3 cut(s) 763, 980, 981
BmeT110I CYCGRG 1 cut(s) 979
BmgT120I GGNCC 1 cut(s) 956
BmiI GGNNCC 3 cut(s) 327, 584, 1052
BmrFI CCNGG 3 cut(s) 763, 980, 981
BmrI ACTGGG 1 cut(s) 671
BmsI GCATC 1 cut(s) 917
BmuI ACTGGG 1 cut(s) 671
Bpu10I CCTNAGC 1 cut(s) 373
BpuMI CCSGG 3 cut(s) 763, 980, 981
BsaJI CCNNGG 3 cut(s) 534, 978, 979
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 701
Bse118I RCCGGY 1 cut(s) 313
Bse1I ACTGG 3 cut(s) 247, 361, 666
Bse3DI GCAATG 3 cut(s) 60, 262, 550
BseDI CCNNGG 3 cut(s) 534, 978, 979
BseGI GGATG 2 cut(s) 932, 1027
BseLI CCNNNNNNNGG 2 cut(s) 212, 701
BseMI GCAATG 3 cut(s) 60, 262, 550
BseMII CTCAG 2 cut(s) 285, 387
BseNI ACTGG 3 cut(s) 247, 361, 666
BseXI GCAGC 5 cut(s) 79, 268, 349, 488, 785
BshFI GGCC 1 cut(s) 957
BsiHKCI CYCGRG 1 cut(s) 979
BsiSI CCGG 3 cut(s) 314, 762, 980
BslI CCNNNNNNNGG 2 cut(s) 212, 701
BsmAI GTCTC 2 cut(s) 213, 820
BsnI GGCC 1 cut(s) 957
BsoBI CYCGRG 1 cut(s) 979
Bsp1286I GDGCHC 1 cut(s) 587
Bsp143I GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
Bsp19I CCATGG 1 cut(s) 534
BspACI CCGC 1 cut(s) 227
BspANI GGCC 1 cut(s) 957
BspCNI CTCAG 2 cut(s) 284, 386
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 3 cut(s) 327, 584, 1052
BspMAI CTGCAG 2 cut(s) 97, 478
BspPI GGATC 6 cut(s) 320, 333, 388, 877, 1045, 1058
BsrDI GCAATG 3 cut(s) 60, 262, 550
BsrFI RCCGGY 1 cut(s) 313
BsrI ACTGG 3 cut(s) 247, 361, 666
BssAI RCCGGY 1 cut(s) 313
BssECI CCNNGG 3 cut(s) 534, 978, 979
BssMI GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
BssNAI GTATAC 1 cut(s) 692
BssT1I CCWWGG 1 cut(s) 534
Bst1107I GTATAC 1 cut(s) 692
Bst4CI ACNGT 2 cut(s) 510, 727
BstC8I GCNNGC 3 cut(s) 315, 714, 751
BstDEI CTNAG 3 cut(s) 271, 373, 578
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 2 cut(s) 932, 1027
BstHHI GCGC 1 cut(s) 874
BstKTI GATC 9 cut(s) 328, 383, 436, 616, 624, 678, 885, 946, 1053
BstMAI GTCTC 2 cut(s) 213, 820
BstMBI GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
BstSCI CCNGG 3 cut(s) 761, 978, 979
BstSFI CTRYAG 5 cut(s) 93, 186, 474, 862, 1032
BstV1I GCAGC 5 cut(s) 79, 268, 349, 488, 785
BstX2I RGATCY 4 cut(s) 325, 613, 943, 1050
BstYI RGATCY 4 cut(s) 325, 613, 943, 1050
BstZ17I GTATAC 1 cut(s) 692
BsuRI GGCC 1 cut(s) 957
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 2 cut(s) 932, 1027
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 2 cut(s) 399, 659
Cac8I GCNNGC 3 cut(s) 315, 714, 751
CciI TCATGA 1 cut(s) 618
CfoI GCGC 1 cut(s) 874
Cfr10I RCCGGY 1 cut(s) 313
Cfr13I GGNCC 1 cut(s) 956
Cfr9I CCCGGG 1 cut(s) 979
Csp6I GTAC 3 cut(s) 850, 984, 1006
CviAII CATG 4 cut(s) 535, 619, 656, 847
CviQI GTAC 3 cut(s) 850, 984, 1006
DdeI CTNAG 3 cut(s) 271, 373, 578
DpnI GATC 9 cut(s) 327, 382, 435, 615, 623, 677, 884, 945, 1052
DpnII GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
DraI TTTAAA 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 587
Eco88I CYCGRG 1 cut(s) 979
EcoT14I CCWWGG 1 cut(s) 534
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 4 cut(s) 538, 622, 659, 850
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FatI CATG 4 cut(s) 534, 618, 655, 846
FauI CCCGC 1 cut(s) 220
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 5 cut(s) 93, 282, 338, 477, 774
FokI GGATG 2 cut(s) 939, 1014
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 5 cut(s) 93, 282, 338, 477, 774
FspBI CTAG 1 cut(s) 123
GlaI GCGC 1 cut(s) 873
GluI GCNGC 5 cut(s) 93, 282, 338, 477, 774
HaeIII GGCC 1 cut(s) 957
HapII CCGG 3 cut(s) 314, 762, 980
HhaI GCGC 1 cut(s) 874
Hin1II CATG 4 cut(s) 538, 622, 659, 850
Hin6I GCGC 1 cut(s) 872
HinP1I GCGC 1 cut(s) 872
HinfI GANTC 4 cut(s) 146, 626, 766, 812
HpaII CCGG 3 cut(s) 314, 762, 980
HphI GGTGA 1 cut(s) 1048
Hpy166II GTNNAC 1 cut(s) 692
Hpy188I TCNGA 4 cut(s) 143, 274, 330, 680
Hpy188III TCNNGA 6 cut(s) 323, 344, 384, 619, 833, 947
Hpy8I GTNNAC 1 cut(s) 692
HpyAV CCTTC 2 cut(s) 39, 1033
HpyCH4III ACNGT 2 cut(s) 510, 727
HpyCH4V TGCA 7 cut(s) 65, 95, 337, 476, 554, 646, 659
HpyF3I CTNAG 3 cut(s) 271, 373, 578
Hsp92II CATG 4 cut(s) 538, 622, 659, 850
HspAI GCGC 1 cut(s) 872
KroI GCCGGC 1 cut(s) 313
KroNI GCCGGC 1 cut(s) 315
Kzo9I GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
LmnI GCTCC 1 cut(s) 582
Lsp1109I GCAGC 5 cut(s) 79, 268, 349, 488, 785
LweI GCATC 1 cut(s) 917
MaeI CTAG 1 cut(s) 123
MaeIII GTNAC 4 cut(s) 97, 423, 965, 989
MalI GATC 9 cut(s) 327, 382, 435, 615, 623, 677, 884, 945, 1052
MboI GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
MboII GAAGA 4 cut(s) 27, 258, 313, 316
MfeI CAATTG 2 cut(s) 555, 699
MflI RGATCY 4 cut(s) 325, 613, 943, 1050
MhlI GDGCHC 1 cut(s) 587
MluCI AATT 9 cut(s) 48, 199, 332, 407, 437, 555, 637, 699, 951
MroNI GCCGGC 1 cut(s) 313
MseI TTAA 4 cut(s) 450, 668, 729, 1080
MspI CCGG 3 cut(s) 314, 762, 980
MspR9I CCNGG 3 cut(s) 763, 980, 981
MunI CAATTG 2 cut(s) 555, 699
NaeI GCCGGC 1 cut(s) 315
NciI CCSGG 3 cut(s) 763, 980, 981
NcoI CCATGG 1 cut(s) 534
NdeII GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
NgoMIV GCCGGC 1 cut(s) 313
NlaIII CATG 4 cut(s) 538, 622, 659, 850
NlaIV GGNNCC 3 cut(s) 327, 584, 1052
NmuCI GTSAC 1 cut(s) 423
PagI TCATGA 1 cut(s) 618
PdiI GCCGGC 1 cut(s) 315
PfeI GAWTC 4 cut(s) 146, 626, 766, 812
PflMI CCANNNNNTGG 2 cut(s) 212, 701
PkrI GCNGC 5 cut(s) 94, 283, 339, 478, 775
PspN4I GGNNCC 3 cut(s) 327, 584, 1052
PspPI GGNCC 1 cut(s) 956
PstI CTGCAG 2 cut(s) 97, 478
PsuI RGATCY 4 cut(s) 325, 613, 943, 1050
RsaI GTAC 3 cut(s) 851, 985, 1007
RsaNI GTAC 3 cut(s) 850, 984, 1006
SaqAI TTAA 4 cut(s) 450, 668, 729, 1080
SatI GCNGC 5 cut(s) 93, 282, 338, 477, 774
Sau3AI GATC 9 cut(s) 325, 380, 433, 613, 621, 675, 882, 943, 1050
Sau96I GGNCC 1 cut(s) 956
ScrFI CCNGG 3 cut(s) 763, 980, 981
SduI GDGCHC 1 cut(s) 587
SfaNI GCATC 1 cut(s) 917
SfcI CTRYAG 5 cut(s) 93, 186, 474, 862, 1032
SmaI CCCGGG 1 cut(s) 981
Sse9I AATT 9 cut(s) 48, 199, 332, 407, 437, 555, 637, 699, 951
SsiI CCGC 1 cut(s) 227
SspMI CTAG 1 cut(s) 123
StyD4I CCNGG 3 cut(s) 761, 978, 979
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 2 cut(s) 510, 727
TaqI TCGA 4 cut(s) 45, 512, 624, 791
TasI AATT 9 cut(s) 48, 199, 332, 407, 437, 555, 637, 699, 951
TatI WGTACW 1 cut(s) 1005
TfiI GAWTC 4 cut(s) 146, 626, 766, 812
Tru1I TTAA 4 cut(s) 450, 668, 729, 1080
Tru9I TTAA 4 cut(s) 450, 668, 729, 1080
TscAI CASTG 2 cut(s) 406, 666
TseFI GTSAC 1 cut(s) 423
TseI GCWGC 5 cut(s) 92, 281, 337, 476, 773
Tsp45I GTSAC 1 cut(s) 423
TspDTI ATGAA 5 cut(s) 21, 27, 504, 644, 1016
TspMI CCCGGG 1 cut(s) 979
TspRI CASTG 2 cut(s) 406, 666
Van91I CCANNNNNTGG 2 cut(s) 212, 701
XapI RAATTY 2 cut(s) 48, 637
XmaI CCCGGG 1 cut(s) 979
XmiI GTMKAC 1 cut(s) 691
XspI CTAG 1 cut(s) 123
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.