RchiOBHm_Chr7g0235831

hydrolase activity, acting on ester bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0000393
Physical Location & Seq
Forward (+)
60766756 .. 60768885
2130 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21133

Sequence Viewer

Length: 750 bp
ATGTCGTCGCCGTTCGTAGGGGTTTTCGCCTTTGGAGATTCGGTTCTTGACACAGGCAACAACAATGACCTCCCTACTGTAGCCAGATGCAATTTCCCACCCTATGGAAGAGATTTTATAGGAGGCATCCCAACGGGAAGATTTAGCAATGGGAAAGTTTACTCCGACATTATAGTGGAAGCCTTGGGTATCAAGCATCTCTTGCCAGCATATCTTGACCCAAACCTACACTCTGAAGATCTCCCAACTGGTGTATGCTTTGCTTCTGGCAATTCCGGTTATGATCCTCTAACACCATCTCTAGTGGATTTATATAGCCTTGGGGCACGACGAATTGCTATTATAAGTGCATTCCCATTGGGATGCGCACCATTGGAAAGAAATGGAGGAGGATTACTAGGAGAGTGTTTAGAGTTGCAAAACCAAAGAGCAAAGATGTTCAACTCTCTATTATCATCCGAATTAGATACCATCAACAGGGACTTCCCTGACGCGAAGCTAGTTTTTCTTGATGTCTACCACCCTTTTCTAGAACTCAATCAACACCCTGAAAACTCAGGATTCGAAGTCTTGAAGATTGGGTGTTGTGGTACTGGAACATTTGAAGTGACATCGTTTTTATGTAACCCATGGAATCCATTCACATGTTCAAATGCATCTAATTACGTATACTGGGATGCAATTCATCCGAGCGAACGAGCTCAAAGAATCGTCGCTTCTCAAACTCTCAAATTGATCACGAGTACGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

249

Amino Acids

27.12

Weight (kDa)

4.93

Isoelectric Point (pI)

27.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 102 - 240 1.9e-10 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 344
Acc16I TGCGCA 1 cut(s) 367
AccB7I CCANNNNNTGG 1 cut(s) 104
AccI GTMKAC 2 cut(s) 516, 669
AccII CGCG 1 cut(s) 494
AclWI GGATC 1 cut(s) 278
AcuI CTGAAG 1 cut(s) 255
AfaI GTAC 2 cut(s) 592, 745
AfiI CCNNNNNNNGG 3 cut(s) 17, 104, 477
AflIII ACRYGT 1 cut(s) 644
AgsI TTSAA 4 cut(s) 442, 574, 605, 651
AloI GAACNNNNNNTCC 2 cut(s) 27, 59
AluBI AGCT 2 cut(s) 499, 701
AluI AGCT 2 cut(s) 499, 701
Alw21I GWGCWC 1 cut(s) 703
AlwI GGATC 1 cut(s) 278
Asp700I GAANNNNTTC 1 cut(s) 638
AspLEI GCGC 1 cut(s) 368
AsuII TTCGAA 1 cut(s) 564
BaeGI GKGCMC 1 cut(s) 328
BanII GRGCYC 1 cut(s) 703
BauI CACGAG 1 cut(s) 739
Bbv12I GWGCWC 1 cut(s) 703
BccI CCATC 2 cut(s) 304, 479
BclI TGATCA 1 cut(s) 735
BfaI CTAG 4 cut(s) 302, 398, 500, 530
BfmI CTRYAG 1 cut(s) 78
BglII AGATCT 1 cut(s) 238
BmrI ACTGGG 1 cut(s) 682
BmsI GCATC 6 cut(s) 77, 135, 205, 353, 665, 667
BmuI ACTGGG 1 cut(s) 682
Bpu14I TTCGAA 1 cut(s) 564
BsaAI YACGTR 2 cut(s) 667, 747
BsaJI CCNNGG 3 cut(s) 183, 319, 629
BsaWI WCCGGW 1 cut(s) 275
BsaXI ACNNNNNCTCC 2 cut(s) 27, 57
Bsc4I CCNNNNNNNGG 3 cut(s) 17, 104, 477
Bse1I ACTGG 3 cut(s) 253, 598, 677
Bse3DI GCAATG 1 cut(s) 154
BseDI CCNNGG 3 cut(s) 183, 319, 629
BseGI GGATG 5 cut(s) 126, 368, 455, 682, 685
BseLI CCNNNNNNNGG 3 cut(s) 17, 104, 477
BseMI GCAATG 1 cut(s) 154
BseMII CTCAG 1 cut(s) 570
BseNI ACTGG 3 cut(s) 253, 598, 677
BseRI GAGGAG 1 cut(s) 402
BseSI GKGCMC 1 cut(s) 328
Bsh1236I CGCG 1 cut(s) 494
BsiHKAI GWGCWC 1 cut(s) 703
BsiSI CCGG 1 cut(s) 276
BslFI GGGAC 1 cut(s) 494
BslI CCNNNNNNNGG 3 cut(s) 17, 104, 477
BsmFI GGGAC 1 cut(s) 494
BsmI GAATGC 1 cut(s) 350
Bsp119I TTCGAA 1 cut(s) 564
Bsp1286I GDGCHC 2 cut(s) 328, 703
Bsp143I GATC 3 cut(s) 238, 283, 735
Bsp19I CCATGG 1 cut(s) 629
BspCNI CTCAG 1 cut(s) 569
BspFNI CGCG 1 cut(s) 494
BspPI GGATC 1 cut(s) 278
BspT104I TTCGAA 1 cut(s) 564
BsrDI GCAATG 1 cut(s) 154
BsrI ACTGG 3 cut(s) 253, 598, 677
BssECI CCNNGG 3 cut(s) 183, 319, 629
BssMI GATC 3 cut(s) 238, 283, 735
BssNAI GTATAC 1 cut(s) 670
BssSI CACGAG 1 cut(s) 739
BssT1I CCWWGG 3 cut(s) 183, 319, 629
Bst1107I GTATAC 1 cut(s) 670
Bst2BI CACGAG 1 cut(s) 739
Bst4CI ACNGT 1 cut(s) 79
Bst6I CTCTTC 1 cut(s) 103
BstAPI GCANNNNNTGC 1 cut(s) 202
BstBAI YACGTR 2 cut(s) 667, 747
BstBI TTCGAA 1 cut(s) 564
BstC8I GCNNGC 1 cut(s) 207
BstDEI CTNAG 1 cut(s) 556
BstDSI CCRYGG 1 cut(s) 629
BstF5I GGATG 5 cut(s) 126, 368, 455, 682, 685
BstFNI CGCG 1 cut(s) 494
BstHHI GCGC 1 cut(s) 368
BstKTI GATC 3 cut(s) 241, 286, 738
BstMBI GATC 3 cut(s) 238, 283, 735
BstMWI GCNNNNNNNGC 1 cut(s) 202
BstNSI RCATGY 1 cut(s) 648
BstSFI CTRYAG 1 cut(s) 78
BstSLI GKGCMC 1 cut(s) 328
BstSNI TACGTA 2 cut(s) 667, 747
BstUI CGCG 1 cut(s) 494
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BstZ17I GTATAC 1 cut(s) 670
BtgI CCRYGG 1 cut(s) 629
BtsCI GGATG 5 cut(s) 126, 368, 455, 682, 685
Cac8I GCNNGC 1 cut(s) 207
CfoI GCGC 1 cut(s) 368
CseI GACGC 1 cut(s) 500
Csp6I GTAC 2 cut(s) 591, 744
CviAII CATG 2 cut(s) 630, 645
CviJI RGCY 5 cut(s) 83, 182, 318, 499, 701
CviKI_1 RGCY 5 cut(s) 83, 182, 318, 499, 701
CviQI GTAC 2 cut(s) 591, 744
DdeI CTNAG 1 cut(s) 556
DpnI GATC 3 cut(s) 240, 285, 737
DpnII GATC 3 cut(s) 238, 283, 735
Eam1104I CTCTTC 1 cut(s) 103
EarI CTCTTC 1 cut(s) 103
Ecl136II GAGCTC 1 cut(s) 701
Eco105I TACGTA 2 cut(s) 667, 747
Eco130I CCWWGG 3 cut(s) 183, 319, 629
Eco24I GRGCYC 1 cut(s) 703
Eco53kI GAGCTC 1 cut(s) 701
Eco57I CTGAAG 1 cut(s) 255
EcoICRI GAGCTC 1 cut(s) 701
EcoT14I CCWWGG 3 cut(s) 183, 319, 629
EcoT22I ATGCAT 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 703
ErhI CCWWGG 3 cut(s) 183, 319, 629
FaeI CATG 2 cut(s) 633, 648
FalI AAGNNNNNCTT 2 cut(s) 185, 217
FaqI GGGAC 1 cut(s) 494
FatI CATG 2 cut(s) 629, 644
FbaI TGATCA 1 cut(s) 735
FblI GTMKAC 2 cut(s) 516, 669
FokI GGATG 5 cut(s) 113, 375, 442, 672, 689
FriOI GRGCYC 1 cut(s) 703
FspAI RTGCGCAY 1 cut(s) 367
FspBI CTAG 4 cut(s) 302, 398, 500, 530
FspI TGCGCA 1 cut(s) 367
GlaI GCGC 1 cut(s) 367
HapII CCGG 1 cut(s) 276
HgaI GACGC 1 cut(s) 500
HhaI GCGC 1 cut(s) 368
Hin1II CATG 2 cut(s) 633, 648
Hin6I GCGC 1 cut(s) 366
HinP1I GCGC 1 cut(s) 366
HinfI GANTC 4 cut(s) 38, 561, 634, 708
HpaII CCGG 1 cut(s) 276
Hpy166II GTNNAC 3 cut(s) 160, 517, 670
Hpy188I TCNGA 4 cut(s) 166, 235, 460, 690
Hpy188III TCNNGA 7 cut(s) 47, 215, 509, 530, 558, 571, 739
Hpy8I GTNNAC 3 cut(s) 160, 517, 670
Hpy99I CGWCG 3 cut(s) 10, 333, 716
HpyCH4III ACNGT 1 cut(s) 79
HpyCH4IV ACGT 2 cut(s) 666, 746
HpyCH4V TGCA 5 cut(s) 90, 350, 418, 656, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 202
HpyF3I CTNAG 1 cut(s) 556
HpySE526I ACGT 2 cut(s) 666, 746
Hsp92II CATG 2 cut(s) 633, 648
HspAI GCGC 1 cut(s) 366
Ksp22I TGATCA 1 cut(s) 735
Kzo9I GATC 3 cut(s) 238, 283, 735
LweI GCATC 6 cut(s) 77, 135, 205, 353, 665, 667
MaeI CTAG 4 cut(s) 302, 398, 500, 530
MaeII ACGT 2 cut(s) 666, 746
MaeIII GTNAC 2 cut(s) 607, 623
MalI GATC 3 cut(s) 240, 285, 737
MboI GATC 3 cut(s) 238, 283, 735
MboII GAAGA 4 cut(s) 120, 150, 248, 586
MflI RGATCY 1 cut(s) 238
MhlI GDGCHC 2 cut(s) 328, 703
MluCI AATT 7 cut(s) 91, 271, 333, 461, 661, 681, 731
MmeI TCCRAC 1 cut(s) 189
MnlI CCTC 5 cut(s) 80, 116, 297, 380, 383
Mph1103I ATGCAT 1 cut(s) 658
MroXI GAANNNNTTC 1 cut(s) 638
MslI CAYNNNNRTG 3 cut(s) 173, 361, 643
MspI CCGG 1 cut(s) 276
Mva1269I GAATGC 1 cut(s) 350
MvnI CGCG 1 cut(s) 494
MwoI GCNNNNNNNGC 1 cut(s) 202
NcoI CCATGG 1 cut(s) 629
NdeII GATC 3 cut(s) 238, 283, 735
NlaIII CATG 2 cut(s) 633, 648
NmuCI GTSAC 1 cut(s) 607
NsbI TGCGCA 1 cut(s) 367
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 1 cut(s) 648
NspV TTCGAA 1 cut(s) 564
PciI ACATGT 1 cut(s) 644
PctI GAATGC 1 cut(s) 350
PdmI GAANNNNTTC 1 cut(s) 638
PfeI GAWTC 4 cut(s) 38, 561, 634, 708
PflMI CCANNNNNTGG 1 cut(s) 104
Ppu21I YACGTR 2 cut(s) 667, 747
PscI ACATGT 1 cut(s) 644
PsiI TTATAA 1 cut(s) 344
Psp124BI GAGCTC 1 cut(s) 703
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 2 cut(s) 592, 745
RsaNI GTAC 2 cut(s) 591, 744
RseI CAYNNNNRTG 3 cut(s) 173, 361, 643
SacI GAGCTC 1 cut(s) 703
Sau3AI GATC 3 cut(s) 238, 283, 735
SduI GDGCHC 2 cut(s) 328, 703
SetI ASST 6 cut(s) 72, 228, 501, 669, 703, 749
SfaNI GCATC 6 cut(s) 77, 135, 205, 353, 665, 667
SfcI CTRYAG 1 cut(s) 78
SfuI TTCGAA 1 cut(s) 564
SmiMI CAYNNNNRTG 3 cut(s) 173, 361, 643
SnaBI TACGTA 2 cut(s) 667, 747
Sse9I AATT 7 cut(s) 91, 271, 333, 461, 661, 681, 731
SspMI CTAG 4 cut(s) 302, 398, 500, 530
SstI GAGCTC 1 cut(s) 703
StyI CCWWGG 3 cut(s) 183, 319, 629
TaaI ACNGT 1 cut(s) 79
TaiI ACGT 2 cut(s) 669, 749
TaqI TCGA 1 cut(s) 564
TasI AATT 7 cut(s) 91, 271, 333, 461, 661, 681, 731
TfiI GAWTC 4 cut(s) 38, 561, 634, 708
TseFI GTSAC 1 cut(s) 607
Tsp45I GTSAC 1 cut(s) 607
TspDTI ATGAA 1 cut(s) 674
Van91I CCANNNNNTGG 1 cut(s) 104
XbaI TCTAGA 1 cut(s) 529
XceI RCATGY 1 cut(s) 648
XmiI GTMKAC 2 cut(s) 516, 669
XmnI GAANNNNTTC 1 cut(s) 638
XspI CTAG 4 cut(s) 302, 398, 500, 530
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.