Rorug02G0075100

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000393
Physical Location & Seq
Forward (+)
5950801 .. 5952623
1823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0075100.1

Sequence Viewer

Length: 810 bp
ATGGGAGTGAGCAACAAAATCACGGCAGTCCTCAACTTCACGGCGCTCCTCTGCTCCATCCCCATAATGGCCGCCGGCATCTGGCTGGCCAACAAGCCCGACAACGAGTGCATCCGCTCTTTCCGGTGGCCAATTTTCGTCCTCGGCGTCCTCATCCTCCTAGTCTCCCTCGCCGGCTTCATCGGGGCCTACTTCAACAAACAGGGCCTTCTTGCTCTCTACCTCTTCTGTATGGCCGCCCTCATAATCCTCCTACTTGTTGTGCTTATATTTGCTTTTACTGTCACGAGGCCCGACGGAAGCTACGGCGTTCCGGGTAGGGCTTACCGGGAGTACCGGCTGGACGGGTTTTCTAAGTGGCTTAGGGATTACGTTACTGATTCCGGGAACTGGCAGAAGATAAGGGTCTGTTTGGCTGAATCGGATGCTTGTCCTAACCTCACTCAGAATTACATCACTGCTGATCAGTTCTTTGTGGCTCACATCTCTCCTCTTCAGTCAGGATGCTGTAAACCTCCAACAGCTTGTGGCTACAACTATGTGAACCCAAATCTGTGGATAAACCCAGTAAACCCTTCAGCAGACCCAGATTGTTTGCTGTGGAACAATGACCAGAACATACTTTGCTACAATTGCAACTCTTGCAGGGCTGGTTTACTAGGAAACTTGAGAAAAGAATGGAGAAGAGCCAATGTGATCCTCATCGTGGCGGTGGTGATTCTCATCTGGGTCTATGTCATTGCCTGCAGCGCCTTCAAGAATGCGCAAACGGAGGAGCTCTTCCGCCGTTACAAGCAGGGATGGGTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

269

Amino Acids

30.24

Weight (kDa)

8.77

Isoelectric Point (pI)

46.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tetraspanin PF00335 5 - 250 2.5e-35 Tetraspanin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 765
AccBSI CCGCTC 1 cut(s) 117
AciI CCGC 5 cut(s) 72, 115, 237, 710, 784
AclWI GGATC 1 cut(s) 691
AcoI YGGCCR 4 cut(s) 69, 87, 128, 234
AcuI CTGAAG 2 cut(s) 479, 561
AcyI GRCGYC 1 cut(s) 147
AfaI GTAC 1 cut(s) 335
AfiI CCNNNNNNNGG 4 cut(s) 67, 81, 390, 706
AgsI TTSAA 2 cut(s) 196, 757
AluBI AGCT 3 cut(s) 303, 524, 778
AluI AGCT 3 cut(s) 303, 524, 778
Alw21I GWGCWC 1 cut(s) 780
Alw26I GTCTC 1 cut(s) 169
AlwI GGATC 1 cut(s) 691
AoxI GGCC 7 cut(s) 69, 87, 128, 186, 205, 234, 290
ApeKI GCWGC 1 cut(s) 747
AspLEI GCGC 3 cut(s) 46, 752, 766
AspS9I GGNCC 3 cut(s) 186, 205, 291
AsuC2I CCSGG 3 cut(s) 315, 329, 385
AsuHPI GGTGA 1 cut(s) 727
BalI TGGCCA 2 cut(s) 89, 130
BanII GRGCYC 1 cut(s) 780
BauI CACGAG 1 cut(s) 286
Bbv12I GWGCWC 1 cut(s) 780
BbvI GCAGC 1 cut(s) 759
BccI CCATC 2 cut(s) 65, 795
BceAI ACGGC 4 cut(s) 39, 57, 322, 771
BclI TGATCA 1 cut(s) 463
BcnI CCSGG 3 cut(s) 315, 329, 385
BcoDI GTCTC 1 cut(s) 169
BfaI CTAG 2 cut(s) 161, 659
BfmI CTRYAG 1 cut(s) 745
BfoI RGCGCY 2 cut(s) 47, 753
BisI GCNGC 3 cut(s) 72, 237, 748
BlsI GCNGC 3 cut(s) 73, 238, 749
Bme1390I CCNGG 3 cut(s) 315, 329, 385
BmgT120I GGNCC 3 cut(s) 186, 205, 291
BmiI GGNNCC 1 cut(s) 187
BmrFI CCNGG 3 cut(s) 315, 329, 385
BmrI ACTGGG 1 cut(s) 560
BmsI GCATC 4 cut(s) 87, 120, 415, 494
BmuI ACTGGG 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 362
BpuEI CTTGAG 1 cut(s) 688
BpuMI CCSGG 3 cut(s) 315, 329, 385
BsaBI GATNNNNATC 2 cut(s) 701, 722
BsaHI GRCGYC 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 142
BsaWI WCCGGW 1 cut(s) 123
Bsc4I CCNNNNNNNGG 4 cut(s) 67, 81, 390, 706
Bse118I RCCGGY 3 cut(s) 74, 173, 336
Bse1I ACTGG 2 cut(s) 395, 566
Bse3DI GCAATG 1 cut(s) 738
Bse8I GATNNNNATC 2 cut(s) 701, 722
BseDI CCNNGG 1 cut(s) 142
BseGI GGATG 6 cut(s) 57, 111, 153, 430, 509, 806
BseJI GATNNNNATC 2 cut(s) 701, 722
BseLI CCNNNNNNNGG 4 cut(s) 67, 81, 390, 706
BseMI GCAATG 1 cut(s) 738
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 2 cut(s) 395, 566
BseRI GAGGAG 3 cut(s) 38, 480, 788
BseXI GCAGC 1 cut(s) 759
BshFI GGCC 7 cut(s) 71, 89, 130, 188, 207, 236, 292
BsiHKAI GWGCWC 1 cut(s) 780
BsiSI CCGG 7 cut(s) 75, 124, 174, 314, 328, 337, 384
BslI CCNNNNNNNGG 4 cut(s) 67, 81, 390, 706
BsmAI GTCTC 1 cut(s) 169
BsmI GAATGC 1 cut(s) 766
BsnI GGCC 7 cut(s) 71, 89, 130, 188, 207, 236, 292
Bsp1286I GDGCHC 1 cut(s) 780
Bsp143I GATC 2 cut(s) 463, 696
BspACI CCGC 5 cut(s) 72, 115, 237, 710, 784
BspANI GGCC 7 cut(s) 71, 89, 130, 188, 207, 236, 292
BspCNI CTCAG 1 cut(s) 457
BspLI GGNNCC 1 cut(s) 187
BspMAI CTGCAG 1 cut(s) 749
BspPI GGATC 1 cut(s) 691
BspQI GCTCTTC 2 cut(s) 679, 785
BsrBI CCGCTC 1 cut(s) 117
BsrDI GCAATG 1 cut(s) 738
BsrFI RCCGGY 3 cut(s) 74, 173, 336
BsrI ACTGG 2 cut(s) 395, 566
BssAI RCCGGY 3 cut(s) 74, 173, 336
BssECI CCNNGG 1 cut(s) 142
BssMI GATC 2 cut(s) 463, 696
BssNI GRCGYC 1 cut(s) 147
BssSI CACGAG 1 cut(s) 286
Bst2BI CACGAG 1 cut(s) 286
Bst4CI ACNGT 1 cut(s) 283
Bst6I CTCTTC 4 cut(s) 230, 498, 679, 785
BstACI GRCGYC 1 cut(s) 147
BstAPI GCANNNNNTGC 1 cut(s) 642
BstC8I GCNNGC 4 cut(s) 76, 87, 175, 745
BstDEI CTNAG 3 cut(s) 354, 362, 444
BstF5I GGATG 6 cut(s) 57, 111, 153, 430, 509, 806
BstH2I RGCGCY 2 cut(s) 47, 753
BstHHI GCGC 3 cut(s) 46, 752, 766
BstKTI GATC 2 cut(s) 466, 699
BstMAI GTCTC 1 cut(s) 169
BstMBI GATC 2 cut(s) 463, 696
BstMWI GCNNNNNNNGC 3 cut(s) 633, 642, 749
BstSCI CCNGG 3 cut(s) 313, 327, 383
BstSFI CTRYAG 1 cut(s) 745
BstV1I GCAGC 1 cut(s) 759
BstXI CCANNNNNNTGG 1 cut(s) 555
BsuRI GGCC 7 cut(s) 71, 89, 130, 188, 207, 236, 292
BtsCI GGATG 6 cut(s) 57, 111, 153, 430, 509, 806
BtsI GCAGTG 1 cut(s) 456
BtsIMutI CAGTG 1 cut(s) 456
Cac8I GCNNGC 4 cut(s) 76, 87, 175, 745
CfoI GCGC 3 cut(s) 46, 752, 766
Cfr10I RCCGGY 3 cut(s) 74, 173, 336
Cfr13I GGNCC 3 cut(s) 186, 205, 291
CseI GACGC 1 cut(s) 136
Csp6I GTAC 1 cut(s) 334
CviQI GTAC 1 cut(s) 334
DdeI CTNAG 3 cut(s) 354, 362, 444
DpnI GATC 2 cut(s) 465, 698
DpnII GATC 2 cut(s) 463, 696
EaeI YGGCCR 4 cut(s) 69, 87, 128, 234
Eam1104I CTCTTC 4 cut(s) 230, 498, 679, 785
EarI CTCTTC 4 cut(s) 230, 498, 679, 785
EciI GGCGGA 1 cut(s) 773
Ecl136II GAGCTC 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 780
Eco53kI GAGCTC 1 cut(s) 778
Eco57I CTGAAG 2 cut(s) 479, 561
EcoICRI GAGCTC 1 cut(s) 778
EcoO109I RGGNCCY 2 cut(s) 186, 205
EcoT38I GRGCYC 1 cut(s) 780
FaiI YATR 7 cut(s) 65, 233, 245, 269, 540, 620, 735
FbaI TGATCA 1 cut(s) 463
Fnu4HI GCNGC 3 cut(s) 72, 237, 748
FokI GGATG 5 cut(s) 44, 98, 140, 437, 516
FriOI GRGCYC 1 cut(s) 780
Fsp4HI GCNGC 3 cut(s) 72, 237, 748
FspBI CTAG 2 cut(s) 161, 659
FspI TGCGCA 1 cut(s) 765
GlaI GCGC 3 cut(s) 45, 751, 765
GluI GCNGC 3 cut(s) 72, 237, 748
HaeII RGCGCY 2 cut(s) 47, 753
HaeIII GGCC 7 cut(s) 71, 89, 130, 188, 207, 236, 292
HapII CCGG 7 cut(s) 75, 124, 174, 314, 328, 337, 384
HgaI GACGC 1 cut(s) 136
HhaI GCGC 3 cut(s) 46, 752, 766
Hin1I GRCGYC 1 cut(s) 147
Hin6I GCGC 3 cut(s) 44, 750, 764
HinP1I GCGC 3 cut(s) 44, 750, 764
HinfI GANTC 3 cut(s) 380, 419, 718
HpaII CCGG 7 cut(s) 75, 124, 174, 314, 328, 337, 384
HphI GGTGA 1 cut(s) 727
Hpy166II GTNNAC 4 cut(s) 512, 544, 571, 656
Hpy188I TCNGA 2 cut(s) 424, 447
Hpy188III TCNNGA 3 cut(s) 286, 501, 757
Hpy8I GTNNAC 4 cut(s) 512, 544, 571, 656
Hpy99I CGWCG 1 cut(s) 299
HpyAV CCTTC 3 cut(s) 218, 585, 763
HpyCH4III ACNGT 1 cut(s) 283
HpyCH4IV ACGT 1 cut(s) 372
HpyCH4V TGCA 4 cut(s) 111, 636, 645, 747
HpyF10VI GCNNNNNNNGC 3 cut(s) 633, 642, 749
HpyF3I CTNAG 3 cut(s) 354, 362, 444
HpySE526I ACGT 1 cut(s) 372
Hsp92I GRCGYC 1 cut(s) 147
HspAI GCGC 3 cut(s) 44, 750, 764
KroI GCCGGC 2 cut(s) 74, 173
KroNI GCCGGC 2 cut(s) 76, 175
Ksp22I TGATCA 1 cut(s) 463
Kzo9I GATC 2 cut(s) 463, 696
LguI GCTCTTC 2 cut(s) 679, 785
LmnI GCTCC 3 cut(s) 51, 59, 775
Lsp1109I GCAGC 1 cut(s) 759
LweI GCATC 4 cut(s) 87, 120, 415, 494
MaeI CTAG 2 cut(s) 161, 659
MaeII ACGT 1 cut(s) 372
MaeIII GTNAC 3 cut(s) 283, 373, 788
MalI GATC 2 cut(s) 465, 698
MbiI CCGCTC 1 cut(s) 117
MboI GATC 2 cut(s) 463, 696
MboII GAAGA 5 cut(s) 217, 409, 485, 696, 772
MfeI CAATTG 1 cut(s) 631
MhlI GDGCHC 1 cut(s) 780
MlsI TGGCCA 2 cut(s) 89, 130
MluCI AATT 3 cut(s) 132, 448, 631
MluNI TGGCCA 2 cut(s) 89, 130
MmeI TCCRAC 1 cut(s) 542
Mox20I TGGCCA 2 cut(s) 89, 130
MroNI GCCGGC 2 cut(s) 74, 173
MscI TGGCCA 2 cut(s) 89, 130
Msp20I TGGCCA 2 cut(s) 89, 130
MspI CCGG 7 cut(s) 75, 124, 174, 314, 328, 337, 384
MspR9I CCNGG 3 cut(s) 315, 329, 385
MunI CAATTG 1 cut(s) 631
Mva1269I GAATGC 1 cut(s) 766
MwoI GCNNNNNNNGC 3 cut(s) 633, 642, 749
NaeI GCCGGC 2 cut(s) 76, 175
NciI CCSGG 3 cut(s) 315, 329, 385
NdeII GATC 2 cut(s) 463, 696
NgoMIV GCCGGC 2 cut(s) 74, 173
NlaIV GGNNCC 1 cut(s) 187
NmeAIII GCCGAG 1 cut(s) 123
NmuCI GTSAC 1 cut(s) 283
NsbI TGCGCA 1 cut(s) 765
PciSI GCTCTTC 2 cut(s) 679, 785
PcsI WCGNNNNNNNCGW 1 cut(s) 144
PctI GAATGC 1 cut(s) 766
PdiI GCCGGC 2 cut(s) 76, 175
PfeI GAWTC 3 cut(s) 380, 419, 718
PfoI TCCNGGA 1 cut(s) 383
PkrI GCNGC 3 cut(s) 73, 238, 749
Psp124BI GAGCTC 1 cut(s) 780
PspN4I GGNNCC 1 cut(s) 187
PspPI GGNCC 3 cut(s) 186, 205, 291
PstI CTGCAG 1 cut(s) 749
RsaI GTAC 1 cut(s) 335
RsaNI GTAC 1 cut(s) 334
SacI GAGCTC 1 cut(s) 780
SapI GCTCTTC 2 cut(s) 679, 785
SatI GCNGC 3 cut(s) 72, 237, 748
Sau3AI GATC 2 cut(s) 463, 696
Sau96I GGNCC 3 cut(s) 186, 205, 291
ScrFI CCNGG 3 cut(s) 315, 329, 385
SduI GDGCHC 1 cut(s) 780
SetI ASST 7 cut(s) 225, 305, 375, 441, 517, 526, 780
SfaNI GCATC 4 cut(s) 87, 120, 415, 494
SfcI CTRYAG 1 cut(s) 745
SmlI CTYRAG 1 cut(s) 667
SmoI CTYRAG 1 cut(s) 667
Sse9I AATT 3 cut(s) 132, 448, 631
SsiI CCGC 5 cut(s) 72, 115, 237, 710, 784
SspMI CTAG 2 cut(s) 161, 659
SstI GAGCTC 1 cut(s) 780
StyD4I CCNGG 3 cut(s) 313, 327, 383
TaaI ACNGT 1 cut(s) 283
TaiI ACGT 1 cut(s) 375
TasI AATT 3 cut(s) 132, 448, 631
TauI GCSGC 2 cut(s) 74, 239
TfiI GAWTC 3 cut(s) 380, 419, 718
TscAI CASTG 1 cut(s) 463
TseFI GTSAC 1 cut(s) 283
TseI GCWGC 1 cut(s) 747
Tsp45I GTSAC 1 cut(s) 283
TspDTI ATGAA 1 cut(s) 169
TspGWI ACGGA 2 cut(s) 312, 785
TspRI CASTG 1 cut(s) 463
XcmI CCANNNNNNNNNTGG 1 cut(s) 64
XspI CTAG 2 cut(s) 161, 659
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.