Rh2AG123000

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000393
Physical Location & Seq
Reverse (-)
11152292 .. 11168587
16296 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG123000.1

Sequence Viewer

Length: 1167 bp
ATGGCTCCTCTCACAAAAAGCTCGTCCTTCATCTTCATCTGTCAAAGCGTTTGCAATGATCTTATGTATATTCCTCTCCTGGCCCATAACGTTGCTGCTGTAACACCTCCCATAAATGAAACAACTCCGGCAGTCTTTACTTTCGGAGATTCAACATTAGATACCGGCAATAACGACTACATCATTTGTATACTCAAAAGTAAGTTCCCACCTTATGGGAGAGACTTTATGGGAGGAAAGCCTACAGGAAGATTTAGCAATGGAAGAGTCCCCTCAGACTTCATTGGTGAAGGATTTGGATTAAAGAAGATTTTGCCGGCTTATCTGGATCCGAATTTGCAGCTTCAAGACCTACTTACTGGTGTATGTTTTGCCTCAGCAGGTTCAGGATATGACCCTCTTACTGCCAGATCACAGTCTGTCTTGTCGTTATCAAATCAATTAAACTTGTTCAAAGAATACAAAAGCAAGATAAACGCAGCAGTTGGGAATGAAAGAACAGCCACTCTACTGTCAAAAAGCTTATTCGTTCTTTCAATAGGAAGCAATGACCTTGCACTTAATTATTTTTCTACACCACTGAGGAGCACTCACTATGATATTCCAGCTTATACAGACTTCCTGCTTGAATTAGCTTCAAACTTCTTTCAGGAACTTTATGCACTGGGAGCAAGAGTGATTGGAGTAGCAAGTATGCCGCCAATTGGGTGTGTGTCAGCACAGAGAACACTTGGTGGAGGCATAGAGAGAGCCTGCGATGAGACTGAGAACCAACCAGCAATCCTCTTCAACTCGAAGCTCTCCGCCCTTATAGACTCCCTCAATAAGAGACTTCCAGAAGCACAACTCATTTACGTTGACATATATAACCCTTTTTTGTCCATTATCCAAGACCCTGCTCAATATGATCAATTAGACTTATTCAAAGAATACAAAAGCCAGATAAACGCAGCAGTTGGGAATGAAACAGCCACTCTACTGTCAGAAAGCTTATTCGTTCTTGCAATAGGAAGCAATGACCTAGCAATTTATTATTATTCTACACCACCGAGGAGTGGTCACTATGATACTCCAGCTTATACAGATCTCCTGCTTGAATCAGCTTCAAATTTCCTTCAGTTGATGTGGGAGAAATTACAGGAACTTATGTACTGGGAGCAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

388

Amino Acids

42.76

Weight (kDa)

4.74

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 45 - 297 8.9e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 371
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 1 cut(s) 190
AciI CCGC 2 cut(s) 698, 804
AclI AACGTT 1 cut(s) 90
AclWI GGATC 2 cut(s) 323, 336
AcsI RAATTY 2 cut(s) 334, 1108
AcuI CTGAAG 1 cut(s) 1100
AdeI CACNNNGTG 1 cut(s) 734
AfaI GTAC 1 cut(s) 1151
AfiI CCNNNNNNNGG 3 cut(s) 215, 704, 1055
AjnI CCWGG 1 cut(s) 78
AluBI AGCT 9 cut(s) 21, 343, 522, 608, 635, 799, 990, 1076, 1103
AluI AGCT 9 cut(s) 21, 343, 522, 608, 635, 799, 990, 1076, 1103
Alw21I GWGCWC 1 cut(s) 590
Alw26I GTCTC 3 cut(s) 216, 755, 823
AlwI GGATC 2 cut(s) 323, 336
AoxI GGCC 1 cut(s) 81
ApeKI GCWGC 4 cut(s) 95, 340, 479, 950
ApoI RAATTY 2 cut(s) 334, 1108
ArsI GACNNNNNNTTYG 2 cut(s) 8, 40
AspS9I GGNCC 1 cut(s) 82
AsuHPI GGTGA 1 cut(s) 299
BamHI GGATCC 1 cut(s) 328
Bbv12I GWGCWC 1 cut(s) 590
BbvCI CCTCAGC 1 cut(s) 376
BbvI GCAGC 4 cut(s) 82, 352, 491, 962
BciT130I CCWGG 1 cut(s) 80
BclI TGATCA 1 cut(s) 907
BcoDI GTCTC 3 cut(s) 216, 755, 823
BfaI CTAG 1 cut(s) 1022
BfmI CTRYAG 1 cut(s) 243
BfuAI ACCTGC 1 cut(s) 371
BglII AGATCT 1 cut(s) 1084
BisI GCNGC 5 cut(s) 96, 341, 480, 698, 951
BlsI GCNGC 5 cut(s) 97, 342, 481, 699, 952
Bme1390I CCNGG 1 cut(s) 80
BmgT120I GGNCC 1 cut(s) 82
BmiI GGNNCC 2 cut(s) 6, 330
BmrFI CCNGG 1 cut(s) 80
BmrI ACTGGG 2 cut(s) 674, 1162
BmuI ACTGGG 2 cut(s) 674, 1162
BplI GAGNNNNNCTC 2 cut(s) 574, 606
BpmI CTGGAG 1 cut(s) 1056
Bpu10I CCTNAGC 1 cut(s) 376
BsaJI CCNNGG 1 cut(s) 1049
BsaXI ACNNNNNCTCC 3 cut(s) 660, 690, 1148
Bsc4I CCNNNNNNNGG 3 cut(s) 215, 704, 1055
Bse118I RCCGGY 2 cut(s) 164, 316
Bse1I ACTGG 3 cut(s) 364, 669, 1157
Bse3DI GCAATG 4 cut(s) 61, 265, 553, 1021
BseBI CCWGG 1 cut(s) 80
BseDI CCNNGG 1 cut(s) 1049
BseLI CCNNNNNNNGG 3 cut(s) 215, 704, 1055
BseMI GCAATG 4 cut(s) 61, 265, 553, 1021
BseMII CTCAG 4 cut(s) 288, 390, 572, 756
BseNI ACTGG 3 cut(s) 364, 669, 1157
BseRI GAGGAG 2 cut(s) 598, 1066
BseXI GCAGC 4 cut(s) 82, 352, 491, 962
BshFI GGCC 1 cut(s) 83
BsiHKAI GWGCWC 1 cut(s) 590
BsiSI CCGG 3 cut(s) 128, 165, 317
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 3 cut(s) 215, 704, 1055
BsmAI GTCTC 3 cut(s) 216, 755, 823
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 1 cut(s) 83
Bsp1286I GDGCHC 1 cut(s) 590
Bsp143I GATC 5 cut(s) 58, 328, 410, 907, 1084
BspACI CCGC 2 cut(s) 698, 804
BspANI GGCC 1 cut(s) 83
BspCNI CTCAG 4 cut(s) 287, 389, 573, 757
BspLI GGNNCC 2 cut(s) 6, 330
BspMI ACCTGC 1 cut(s) 371
BspPI GGATC 2 cut(s) 323, 336
BsrDI GCAATG 4 cut(s) 61, 265, 553, 1021
BsrFI RCCGGY 2 cut(s) 164, 316
BsrI ACTGG 3 cut(s) 364, 669, 1157
BssAI RCCGGY 2 cut(s) 164, 316
BssECI CCNNGG 1 cut(s) 1049
BssMI GATC 5 cut(s) 58, 328, 410, 907, 1084
BssNAI GTATAC 1 cut(s) 191
Bst1107I GTATAC 1 cut(s) 191
Bst2UI CCWGG 1 cut(s) 80
Bst4CI ACNGT 3 cut(s) 417, 513, 981
Bst6I CTCTTC 2 cut(s) 259, 791
BstC8I GCNNGC 2 cut(s) 318, 754
BstDEI CTNAG 4 cut(s) 274, 376, 581, 765
BstKTI GATC 5 cut(s) 61, 331, 413, 910, 1087
BstMAI GTCTC 3 cut(s) 216, 755, 823
BstMBI GATC 5 cut(s) 58, 328, 410, 907, 1084
BstMWI GCNNNNNNNGC 1 cut(s) 668
BstNI CCWGG 1 cut(s) 80
BstSCI CCNGG 1 cut(s) 78
BstSFI CTRYAG 1 cut(s) 243
BstV1I GCAGC 4 cut(s) 82, 352, 491, 962
BstX2I RGATCY 2 cut(s) 328, 1084
BstYI RGATCY 2 cut(s) 328, 1084
BstZ17I GTATAC 1 cut(s) 191
BsuRI GGCC 1 cut(s) 83
BtgZI GCGATG 1 cut(s) 771
BtsIMutI CAGTG 2 cut(s) 578, 662
BveI ACCTGC 1 cut(s) 371
Cac8I GCNNGC 2 cut(s) 318, 754
Cfr10I RCCGGY 2 cut(s) 164, 316
Cfr13I GGNCC 1 cut(s) 82
Csp6I GTAC 1 cut(s) 1150
CviQI GTAC 1 cut(s) 1150
DdeI CTNAG 4 cut(s) 274, 376, 581, 765
DpnI GATC 5 cut(s) 60, 330, 412, 909, 1086
DpnII GATC 5 cut(s) 58, 328, 410, 907, 1084
DraIII CACNNNGTG 1 cut(s) 734
Eam1104I CTCTTC 2 cut(s) 259, 791
EarI CTCTTC 2 cut(s) 259, 791
EciI GGCGGA 1 cut(s) 793
Eco57I CTGAAG 1 cut(s) 1100
EcoRII CCWGG 1 cut(s) 78
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 1 cut(s) 254
FbaI TGATCA 1 cut(s) 907
FblI GTMKAC 1 cut(s) 190
Fnu4HI GCNGC 5 cut(s) 96, 341, 480, 698, 951
Fsp4HI GCNGC 5 cut(s) 96, 341, 480, 698, 951
FspBI CTAG 1 cut(s) 1022
GluI GCNGC 5 cut(s) 96, 341, 480, 698, 951
GsuI CTGGAG 1 cut(s) 1056
HaeIII GGCC 1 cut(s) 83
HapII CCGG 3 cut(s) 128, 165, 317
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HindIII AAGCTT 2 cut(s) 520, 988
HinfI GANTC 4 cut(s) 149, 267, 815, 1097
HpaII CCGG 3 cut(s) 128, 165, 317
HphI GGTGA 1 cut(s) 299
Hpy166II GTNNAC 2 cut(s) 191, 859
Hpy188I TCNGA 4 cut(s) 146, 277, 333, 985
Hpy188III TCNNGA 5 cut(s) 326, 347, 387, 650, 836
Hpy8I GTNNAC 2 cut(s) 191, 859
HpyAV CCTTC 3 cut(s) 37, 284, 1124
HpyCH4III ACNGT 3 cut(s) 417, 513, 981
HpyCH4IV ACGT 2 cut(s) 90, 855
HpyCH4V TGCA 5 cut(s) 54, 340, 557, 662, 1004
HpyF10VI GCNNNNNNNGC 1 cut(s) 668
HpyF3I CTNAG 4 cut(s) 274, 376, 581, 765
HpySE526I ACGT 2 cut(s) 90, 855
KroI GCCGGC 1 cut(s) 316
KroNI GCCGGC 1 cut(s) 318
Ksp22I TGATCA 1 cut(s) 907
Kzo9I GATC 5 cut(s) 58, 328, 410, 907, 1084
LmnI GCTCC 4 cut(s) 10, 585, 668, 1156
Lsp1109I GCAGC 4 cut(s) 82, 352, 491, 962
MaeI CTAG 1 cut(s) 1022
MaeII ACGT 2 cut(s) 90, 855
MaeIII GTNAC 2 cut(s) 100, 1058
MalI GATC 5 cut(s) 60, 330, 412, 909, 1086
MboI GATC 5 cut(s) 58, 328, 410, 907, 1084
MboII GAAGA 5 cut(s) 25, 261, 276, 319, 778
MfeI CAATTG 1 cut(s) 702
MflI RGATCY 2 cut(s) 328, 1084
MhlI GDGCHC 1 cut(s) 590
MluCI AATT 9 cut(s) 334, 440, 562, 629, 702, 911, 1026, 1108, 1133
MlyI GAGTC 2 cut(s) 276, 809
MroNI GCCGGC 1 cut(s) 316
MseI TTAA 3 cut(s) 302, 443, 561
MspI CCGG 3 cut(s) 128, 165, 317
MspR9I CCNGG 1 cut(s) 80
MunI CAATTG 1 cut(s) 702
MvaI CCWGG 1 cut(s) 80
MwoI GCNNNNNNNGC 1 cut(s) 668
NaeI GCCGGC 1 cut(s) 318
NdeII GATC 5 cut(s) 58, 328, 410, 907, 1084
NgoMIV GCCGGC 1 cut(s) 316
NlaIV GGNNCC 2 cut(s) 6, 330
NmuCI GTSAC 1 cut(s) 1058
PdiI GCCGGC 1 cut(s) 318
PfeI GAWTC 2 cut(s) 149, 1097
PflMI CCANNNNNTGG 1 cut(s) 215
PkrI GCNGC 5 cut(s) 97, 342, 481, 699, 952
PleI GAGTC 2 cut(s) 275, 809
PpsI GAGTC 2 cut(s) 275, 809
Psp1406I AACGTT 1 cut(s) 90
Psp6I CCWGG 1 cut(s) 78
PspGI CCWGG 1 cut(s) 78
PspN4I GGNNCC 2 cut(s) 6, 330
PspPI GGNCC 1 cut(s) 82
PsuI RGATCY 2 cut(s) 328, 1084
RsaI GTAC 1 cut(s) 1151
RsaNI GTAC 1 cut(s) 1150
SaqAI TTAA 3 cut(s) 302, 443, 561
SatI GCNGC 5 cut(s) 96, 341, 480, 698, 951
Sau3AI GATC 5 cut(s) 58, 328, 410, 907, 1084
Sau96I GGNCC 1 cut(s) 82
SchI GAGTC 2 cut(s) 276, 809
ScrFI CCNGG 1 cut(s) 80
SduI GDGCHC 1 cut(s) 590
SfcI CTRYAG 1 cut(s) 243
Sse9I AATT 9 cut(s) 334, 440, 562, 629, 702, 911, 1026, 1108, 1133
SsiI CCGC 2 cut(s) 698, 804
SspMI CTAG 1 cut(s) 1022
StyD4I CCNGG 1 cut(s) 78
TaaI ACNGT 3 cut(s) 417, 513, 981
TaiI ACGT 2 cut(s) 93, 858
TaqI TCGA 1 cut(s) 794
TasI AATT 9 cut(s) 334, 440, 562, 629, 702, 911, 1026, 1108, 1133
TatI WGTACW 1 cut(s) 1149
TauI GCSGC 1 cut(s) 700
TfiI GAWTC 2 cut(s) 149, 1097
Tru1I TTAA 3 cut(s) 302, 443, 561
Tru9I TTAA 3 cut(s) 302, 443, 561
TscAI CASTG 2 cut(s) 585, 669
TseFI GTSAC 1 cut(s) 1058
TseI GCWGC 4 cut(s) 95, 340, 479, 950
Tsp45I GTSAC 1 cut(s) 1058
TspDTI ATGAA 6 cut(s) 19, 25, 132, 271, 507, 978
TspRI CASTG 2 cut(s) 585, 669
Van91I CCANNNNNTGG 1 cut(s) 215
XapI RAATTY 2 cut(s) 334, 1108
XmiI GTMKAC 1 cut(s) 190
XspI CTAG 1 cut(s) 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.