MD02G1127000.v1.1

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000393
Physical Location & Seq
Forward (+)
10268242 .. 10270630
2389 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1127000.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGCAGTGTATATTTTCACCCACCAAGGCAATGAACTTCCTCTCCCCAAAACTTCTTCCTTCATCGTCTAAATCTTCTCTCAAACTTATAATGATCACCATAGTCTTGTTCTTGTACCGCAATGTTGCTGCTGTAGAGCTACCAAACAACGAAAAGATTCCTGCAGTGATTGTTTTTGGAGATTCAATAGTGGATCCGGGAAACAACAACAATATTGGCACGATAGTCAAATGCAATTTCCCACCTTATGGGAGGGACTTTGTAGGAAAAAGGCCTACTGGAAGGTTTAGCAATGGCAGAGTCCCCTCAGACTGGATAGCTGAATCAGTTGGAGTGAAGAAGATTTTGCCAGCTTATTTGGACTCAAATCTGAAGATTCAAGACCTACTTACTGGTGTAAGTTTTGCCTCAGGTGGCTCAGGATATGATCCCCTCACTCCCAAAATAGTGATAAATGCAGCCGTTGGGAAGGAGAGAACAACGACTATAGTATCAAAAAGCATATACATTGTGTGCACAGGGAGTAACGACATTGCGAATACTTATTTATCTACACCACTAAGAAGATCTCACTATGACATTCCAGCGTATACCGATCTCATGGTTAAATCGGCTTCAAGTTTCTTTCAGGAACTATATGGACTCGGAGCAAGAAGAATTGGAGTTGTAAGTCTGCCAGCCATCGGGTGTGTACCGTCACAGAGAACACTGAGTGGAGGCATAGACAGAGGGTGCTCGGAGACTGCCAACCAAGCAGCAAGTCTCTTCAACTCCAAGCTCTCCGCCCAAATAGATGCCTTCAATAGGAGGCTGCCAGAAGCAAGGCTTGTCTACCTCGATATCTATAACACATTGCTTTCCCTCATCCAAAACCCTACTCAATATGGATTTGAAGTGATCAATCGAGGATGTTGTGGGACAGGAAACGTTGAGGTTAGCATTCTGTGTACACGTTACTCTCCGGGAACTTGCAATGATTCGTCGAAATACATATTCTGGGACAGCTACCATCCTTCAGAGAAAGGATATGAAGTCATCACCTCTCTGGTTTTTGATTCGCAAGTTCATAAGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

39.24

Weight (kDa)

8.95

Isoelectric Point (pI)

34.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 56 - 348 5.3e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 89
AccB7I CCANNNNNTGG 1 cut(s) 248
AccI GTMKAC 2 cut(s) 590, 831
AciI CCGC 2 cut(s) 118, 783
AclI AACGTT 1 cut(s) 927
AclWI GGATC 3 cut(s) 188, 201, 422
AcuI CTGAAG 2 cut(s) 392, 999
AdeI CACNNNGTG 1 cut(s) 713
AfaI GTAC 3 cut(s) 116, 693, 949
AfiI CCNNNNNNNGG 4 cut(s) 248, 312, 683, 804
AflIII ACRYGT 1 cut(s) 950
AgsI TTSAA 6 cut(s) 186, 380, 618, 769, 802, 893
AloI GAACNNNNNNTCC 2 cut(s) 26, 58
AluBI AGCT 5 cut(s) 139, 320, 353, 778, 1005
AluI AGCT 5 cut(s) 139, 320, 353, 778, 1005
Alw21I GWGCWC 2 cut(s) 518, 737
Alw26I GTCTC 2 cut(s) 734, 767
Alw44I GTGCAC 1 cut(s) 514
AlwI GGATC 3 cut(s) 188, 201, 422
AoxI GGCC 1 cut(s) 272
ApaLI GTGCAC 1 cut(s) 514
ApeKI GCWGC 4 cut(s) 128, 458, 755, 811
Asp700I GAANNNNTTC 1 cut(s) 156
AsuC2I CCSGG 2 cut(s) 198, 963
AsuHPI GGTGA 3 cut(s) 9, 88, 1030
AxyI CCTNAGG 1 cut(s) 409
BaeGI GKGCMC 1 cut(s) 518
BaeI ACNNNNGTAYC 2 cut(s) 474, 507
BamHI GGATCC 1 cut(s) 193
Bbv12I GWGCWC 2 cut(s) 518, 737
BbvI GCAGC 4 cut(s) 115, 470, 767, 798
BccI CCATC 2 cut(s) 689, 1017
BceAI ACGGC 1 cut(s) 446
BclI TGATCA 2 cut(s) 93, 897
BcnI CCSGG 2 cut(s) 198, 963
BcoDI GTCTC 2 cut(s) 734, 767
BfmI CTRYAG 3 cut(s) 132, 162, 486
BglII AGATCT 1 cut(s) 566
BisI GCNGC 4 cut(s) 129, 459, 756, 812
BlsI GCNGC 4 cut(s) 130, 460, 757, 813
Bme1390I CCNGG 2 cut(s) 198, 963
BmiI GGNNCC 1 cut(s) 195
BmrFI CCNGG 2 cut(s) 198, 963
BmsI GCATC 1 cut(s) 784
Bpu10I CCTNAGC 1 cut(s) 418
BpuMI CCSGG 2 cut(s) 198, 963
BsaJI CCNNGG 1 cut(s) 24
BsaXI ACNNNNNCTCC 2 cut(s) 26, 56
Bsc4I CCNNNNNNNGG 4 cut(s) 248, 312, 683, 804
Bse1I ACTGG 3 cut(s) 283, 317, 397
Bse21I CCTNAGG 1 cut(s) 409
Bse3DI GCAATG 6 cut(s) 36, 127, 298, 531, 851, 979
BseDI CCNNGG 1 cut(s) 24
BseGI GGATG 3 cut(s) 864, 914, 1009
BseLI CCNNNNNNNGG 4 cut(s) 248, 312, 683, 804
BseMI GCAATG 6 cut(s) 36, 127, 298, 531, 851, 979
BseMII CTCAG 4 cut(s) 321, 423, 432, 701
BseNI ACTGG 3 cut(s) 283, 317, 397
BseSI GKGCMC 1 cut(s) 518
BseXI GCAGC 4 cut(s) 115, 470, 767, 798
BshFI GGCC 1 cut(s) 274
BsiHKAI GWGCWC 2 cut(s) 518, 737
BsiSI CCGG 2 cut(s) 197, 962
BslFI GGGAC 4 cut(s) 269, 287, 931, 1013
BslI CCNNNNNNNGG 4 cut(s) 248, 312, 683, 804
BsmAI GTCTC 2 cut(s) 734, 767
BsmFI GGGAC 4 cut(s) 269, 287, 931, 1013
BsmI GAATGC 1 cut(s) 939
BsnI GGCC 1 cut(s) 274
Bsp1286I GDGCHC 2 cut(s) 518, 737
Bsp1407I TGTACA 1 cut(s) 947
Bsp143I GATC 6 cut(s) 93, 193, 427, 566, 595, 897
BspACI CCGC 2 cut(s) 118, 783
BspANI GGCC 1 cut(s) 274
BspCNI CTCAG 4 cut(s) 320, 422, 431, 702
BspLI GGNNCC 1 cut(s) 195
BspMAI CTGCAG 1 cut(s) 166
BspPI GGATC 3 cut(s) 188, 201, 422
BsrDI GCAATG 6 cut(s) 36, 127, 298, 531, 851, 979
BsrGI TGTACA 1 cut(s) 947
BsrI ACTGG 3 cut(s) 283, 317, 397
BssECI CCNNGG 1 cut(s) 24
BssMI GATC 6 cut(s) 93, 193, 427, 566, 595, 897
BssNAI GTATAC 1 cut(s) 591
BssT1I CCWWGG 1 cut(s) 24
Bst1107I GTATAC 1 cut(s) 591
Bst4CI ACNGT 1 cut(s) 696
Bst6I CTCTTC 1 cut(s) 770
BstAUI TGTACA 1 cut(s) 947
BstC8I GCNNGC 2 cut(s) 351, 678
BstDEI CTNAG 5 cut(s) 307, 409, 418, 560, 710
BstENI CCTNNNNNAGG 1 cut(s) 802
BstF5I GGATG 3 cut(s) 864, 914, 1009
BstKTI GATC 6 cut(s) 96, 196, 430, 569, 598, 900
BstMAI GTCTC 2 cut(s) 734, 767
BstMBI GATC 6 cut(s) 93, 193, 427, 566, 595, 897
BstMWI GCNNNNNNNGC 1 cut(s) 752
BstSCI CCNGG 2 cut(s) 196, 961
BstSFI CTRYAG 3 cut(s) 132, 162, 486
BstSLI GKGCMC 1 cut(s) 518
BstV1I GCAGC 4 cut(s) 115, 470, 767, 798
BstX2I RGATCY 2 cut(s) 193, 566
BstYI RGATCY 2 cut(s) 193, 566
BstZ17I GTATAC 1 cut(s) 591
Bsu36I CCTNAGG 1 cut(s) 409
BsuRI GGCC 1 cut(s) 274
BtsCI GGATG 3 cut(s) 864, 914, 1009
BtsI GCAGTG 2 cut(s) 11, 171
BtsIMutI CAGTG 3 cut(s) 11, 171, 707
Cac8I GCNNGC 2 cut(s) 351, 678
Csp6I GTAC 3 cut(s) 115, 692, 948
CviAII CATG 1 cut(s) 601
CviQI GTAC 3 cut(s) 115, 692, 948
DdeI CTNAG 5 cut(s) 307, 409, 418, 560, 710
DpnI GATC 6 cut(s) 95, 195, 429, 568, 597, 899
DpnII GATC 6 cut(s) 93, 193, 427, 566, 595, 897
DraIII CACNNNGTG 1 cut(s) 713
Eam1104I CTCTTC 1 cut(s) 770
EarI CTCTTC 1 cut(s) 770
EciI GGCGGA 1 cut(s) 772
Eco130I CCWWGG 1 cut(s) 24
Eco147I AGGCCT 1 cut(s) 274
Eco32I GATATC 1 cut(s) 841
Eco57I CTGAAG 2 cut(s) 392, 999
Eco81I CCTNAGG 1 cut(s) 409
EcoNI CCTNNNNNAGG 1 cut(s) 802
EcoRV GATATC 1 cut(s) 841
EcoT14I CCWWGG 1 cut(s) 24
ErhI CCWWGG 1 cut(s) 24
FaeI CATG 1 cut(s) 604
FalI AAGNNNNNCTT 4 cut(s) 372, 404, 810, 842
FaqI GGGAC 4 cut(s) 269, 287, 931, 1013
FatI CATG 1 cut(s) 600
FbaI TGATCA 2 cut(s) 93, 897
FblI GTMKAC 2 cut(s) 590, 831
Fnu4HI GCNGC 4 cut(s) 129, 459, 756, 812
FokI GGATG 3 cut(s) 851, 921, 996
Fsp4HI GCNGC 4 cut(s) 129, 459, 756, 812
GluI GCNGC 4 cut(s) 129, 459, 756, 812
HaeIII GGCC 1 cut(s) 274
HapII CCGG 2 cut(s) 197, 962
Hin1II CATG 1 cut(s) 604
HinfI GANTC 9 cut(s) 157, 182, 300, 323, 362, 376, 642, 977, 1055
HpaII CCGG 2 cut(s) 197, 962
HphI GGTGA 3 cut(s) 9, 88, 1030
Hpy166II GTNNAC 6 cut(s) 516, 591, 692, 832, 948, 950
Hpy188I TCNGA 6 cut(s) 310, 372, 647, 739, 1018, 1079
Hpy188III TCNNGA 3 cut(s) 380, 420, 629
Hpy8I GTNNAC 6 cut(s) 516, 591, 692, 832, 948, 950
Hpy99I CGWCG 1 cut(s) 985
HpyAV CCTTC 5 cut(s) 69, 276, 463, 808, 1023
HpyCH4III ACNGT 1 cut(s) 696
HpyCH4IV ACGT 2 cut(s) 927, 952
HpyCH4V TGCA 6 cut(s) 4, 164, 234, 458, 516, 972
HpyF10VI GCNNNNNNNGC 1 cut(s) 752
HpyF3I CTNAG 5 cut(s) 307, 409, 418, 560, 710
HpySE526I ACGT 2 cut(s) 927, 952
Hsp92II CATG 1 cut(s) 604
Ksp22I TGATCA 2 cut(s) 93, 897
Kzo9I GATC 6 cut(s) 93, 193, 427, 566, 595, 897
LmnI GCTCC 1 cut(s) 647
Lsp1109I GCAGC 4 cut(s) 115, 470, 767, 798
LweI GCATC 1 cut(s) 784
MaeII ACGT 2 cut(s) 927, 952
MaeIII GTNAC 3 cut(s) 524, 696, 953
MalI GATC 6 cut(s) 95, 195, 429, 568, 597, 899
MboI GATC 6 cut(s) 93, 193, 427, 566, 595, 897
MboII GAAGA 9 cut(s) 47, 66, 349, 352, 385, 576, 666, 757, 1066
MflI RGATCY 2 cut(s) 193, 566
MhlI GDGCHC 2 cut(s) 518, 737
MluCI AATT 2 cut(s) 235, 657
MlyI GAGTC 3 cut(s) 309, 356, 636
MmeI TCCRAC 1 cut(s) 310
MroXI GAANNNNTTC 1 cut(s) 156
MseI TTAA 1 cut(s) 606
MspI CCGG 2 cut(s) 197, 962
MspR9I CCNGG 2 cut(s) 198, 963
Mva1269I GAATGC 1 cut(s) 939
MwoI GCNNNNNNNGC 1 cut(s) 752
NciI CCSGG 2 cut(s) 198, 963
NdeII GATC 6 cut(s) 93, 193, 427, 566, 595, 897
NlaIII CATG 1 cut(s) 604
NlaIV GGNNCC 1 cut(s) 195
NmuCI GTSAC 1 cut(s) 696
PceI AGGCCT 1 cut(s) 274
PctI GAATGC 1 cut(s) 939
PdmI GAANNNNTTC 1 cut(s) 156
PfeI GAWTC 6 cut(s) 157, 182, 323, 376, 977, 1055
PflMI CCANNNNNTGG 1 cut(s) 248
PfoI TCCNGGA 2 cut(s) 196, 961
PkrI GCNGC 4 cut(s) 130, 460, 757, 813
PleI GAGTC 3 cut(s) 308, 356, 636
PpsI GAGTC 3 cut(s) 308, 356, 636
PsiI TTATAA 1 cut(s) 89
Psp1406I AACGTT 1 cut(s) 927
PspN4I GGNNCC 1 cut(s) 195
PstI CTGCAG 1 cut(s) 166
PsuI RGATCY 2 cut(s) 193, 566
RsaI GTAC 3 cut(s) 116, 693, 949
RsaNI GTAC 3 cut(s) 115, 692, 948
SaqAI TTAA 1 cut(s) 606
SatI GCNGC 4 cut(s) 129, 459, 756, 812
Sau3AI GATC 6 cut(s) 93, 193, 427, 566, 595, 897
SchI GAGTC 3 cut(s) 309, 356, 636
ScrFI CCNGG 2 cut(s) 198, 963
SduI GDGCHC 2 cut(s) 518, 737
SfaNI GCATC 1 cut(s) 784
SfcI CTRYAG 3 cut(s) 132, 162, 486
Sse9I AATT 2 cut(s) 235, 657
SseBI AGGCCT 1 cut(s) 274
SsiI CCGC 2 cut(s) 118, 783
SspI AATATT 1 cut(s) 214
StuI AGGCCT 1 cut(s) 274
StyD4I CCNGG 2 cut(s) 196, 961
StyI CCWWGG 1 cut(s) 24
TaaI ACNGT 1 cut(s) 696
TaiI ACGT 2 cut(s) 930, 955
TaqI TCGA 3 cut(s) 837, 904, 983
TasI AATT 2 cut(s) 235, 657
TatI WGTACW 1 cut(s) 947
TfiI GAWTC 6 cut(s) 157, 182, 323, 376, 977, 1055
Tru1I TTAA 1 cut(s) 606
Tru9I TTAA 1 cut(s) 606
TscAI CASTG 2 cut(s) 171, 714
TseFI GTSAC 1 cut(s) 696
TseI GCWGC 4 cut(s) 128, 458, 755, 811
Tsp45I GTSAC 1 cut(s) 696
TspDTI ATGAA 4 cut(s) 47, 51, 1044, 1055
TspRI CASTG 2 cut(s) 171, 714
Van91I CCANNNNNTGG 1 cut(s) 248
VneI GTGCAC 1 cut(s) 514
XagI CCTNNNNNAGG 1 cut(s) 802
XmiI GTMKAC 2 cut(s) 590, 831
XmnI GAANNNNTTC 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.