MD15G1241600.v1.1

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000393
Physical Location & Seq
Reverse (-)
19911663 .. 19913772
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1241600.v1.1.491

Sequence Viewer

Length: 873 bp
ATGATTTCTCTCTTCCAAGTACTTCATCAGACATCTAATTCATCGGTCAAGTTTCTTTCAATCGTCATAGTTTTATTCCTCTTCCATTATGCATCTGGAACTGCTGTAAAACTACCCGAAAATAAAAAGATTCCAGCGGTGATTGTTTTCGGTAATTCAATAGTGGATCCTGGAAACAACAACAATATCAAAACTACAGTAAAAGCCAACTTCCCGCCATATGGGAGGGACTTCATAGAACGGAGGCCTACTGGAAGGTTCAACAATGGCAGAGTCCCCTCAGACTTTATTGCTGAATCAGCTGGAGTGAAGAAGATTTTGCCACCTTATCTGGATCCAAATCTGAGTCTTCAAGACCTACTTACTGGTGTAAGTTTTGCCTCAGGTGGTTCAGGATATGATCCCCTCTCTTCCCAAATAGTGGAACTATATGGACTGGGAGCAAGAAGAATTGGAGTACTAAGTTTGCTGGCAATCGGGTGTGTGCCATCACAGAGAACATTGAGGGGAGGCATACATAGGGAGTGCTCAGAGCCTCAAAACCAAGCAGCAATCCTCTTCAACTCGAAGCTTTCGGCCCAAATAGATGCCTTCAATAAGAAGCTTCCAGAAGCAAGGCTTGTCTACCTTGATATCTATTACACATTGCTTTTCCTCATCCTAAACCCTGCTCAATATGGATTTGAAGTGGCGAATAGGGGATGTTGTGGAACAAGAAATATTGAGGTTAGCCTTACGTGTAATCTTCACACTCCAGGAACCTACAACGATTCGTTGAAATGCATATTCTGGGATAGCTATCATCTTTCAGAGAAGACATATGCAATCCTTAACCCTCTAGTTTTCGACACGCAAGTTCGTAAATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

291

Amino Acids

32.12

Weight (kDa)

9.07

Isoelectric Point (pI)

38.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 136 - 277 7.2e-13 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 421
AccI GTMKAC 1 cut(s) 624
AciI CCGC 2 cut(s) 137, 215
AclWI GGATC 5 cut(s) 161, 174, 329, 342, 395
AcsI RAATTY 1 cut(s) 863
AfaI GTAC 2 cut(s) 21, 459
AfiI CCNNNNNNNGG 2 cut(s) 221, 421
AflIII ACRYGT 1 cut(s) 737
AgsI TTSAA 8 cut(s) 60, 159, 262, 353, 562, 595, 686, 778
AjnI CCWGG 2 cut(s) 169, 754
AluBI AGCT 4 cut(s) 302, 571, 604, 798
AluI AGCT 4 cut(s) 302, 571, 604, 798
Alw21I GWGCWC 1 cut(s) 530
AlwI GGATC 5 cut(s) 161, 174, 329, 342, 395
AoxI GGCC 2 cut(s) 245, 576
ApeKI GCWGC 1 cut(s) 548
ApoI RAATTY 1 cut(s) 863
AspS9I GGNCC 1 cut(s) 577
AsuHPI GGTGA 1 cut(s) 151
AxyI CCTNAGG 1 cut(s) 382
BamHI GGATCC 2 cut(s) 166, 334
BbsI GAAGAC 2 cut(s) 341, 821
Bbv12I GWGCWC 1 cut(s) 530
BbvI GCAGC 1 cut(s) 560
BccI CCATC 1 cut(s) 496
BciT130I CCWGG 2 cut(s) 171, 756
BfaI CTAG 1 cut(s) 839
BfmI CTRYAG 1 cut(s) 195
BisI GCNGC 1 cut(s) 549
BlsI GCNGC 1 cut(s) 550
BmcAI AGTACT 2 cut(s) 21, 459
Bme1390I CCNGG 2 cut(s) 171, 756
BmgT120I GGNCC 1 cut(s) 577
BmiI GGNNCC 3 cut(s) 168, 336, 760
BmrFI CCNGG 2 cut(s) 171, 756
BmrI ACTGGG 1 cut(s) 446
BmsI GCATC 2 cut(s) 101, 577
BmuI ACTGGG 1 cut(s) 446
BpiI GAAGAC 2 cut(s) 341, 821
BpmI CTGGAG 2 cut(s) 324, 738
BsaAI YACGTR 1 cut(s) 738
BsaBI GATNNNNATC 2 cut(s) 339, 798
Bsc4I CCNNNNNNNGG 2 cut(s) 221, 421
Bse1I ACTGG 3 cut(s) 256, 370, 441
Bse21I CCTNAGG 1 cut(s) 382
Bse3DI GCAATG 1 cut(s) 644
Bse8I GATNNNNATC 2 cut(s) 339, 798
BseBI CCWGG 2 cut(s) 171, 756
BseGI GGATG 2 cut(s) 657, 707
BseJI GATNNNNATC 2 cut(s) 339, 798
BseLI CCNNNNNNNGG 2 cut(s) 221, 421
BseMI GCAATG 1 cut(s) 644
BseMII CTCAG 4 cut(s) 294, 335, 396, 543
BseNI ACTGG 3 cut(s) 256, 370, 441
BseXI GCAGC 1 cut(s) 560
BshFI GGCC 2 cut(s) 247, 578
BsiHKAI GWGCWC 1 cut(s) 530
BslFI GGGAC 2 cut(s) 242, 260
BslI CCNNNNNNNGG 2 cut(s) 221, 421
BsmFI GGGAC 2 cut(s) 242, 260
BsnI GGCC 2 cut(s) 247, 578
Bsp1286I GDGCHC 1 cut(s) 530
Bsp143I GATC 3 cut(s) 166, 334, 400
BspACI CCGC 2 cut(s) 137, 215
BspANI GGCC 2 cut(s) 247, 578
BspCNI CTCAG 4 cut(s) 293, 336, 395, 542
BspLI GGNNCC 3 cut(s) 168, 336, 760
BspPI GGATC 5 cut(s) 161, 174, 329, 342, 395
BsrDI GCAATG 1 cut(s) 644
BsrI ACTGG 3 cut(s) 256, 370, 441
BssMI GATC 3 cut(s) 166, 334, 400
Bst2UI CCWGG 2 cut(s) 171, 756
Bst4CI ACNGT 1 cut(s) 199
Bst6I CTCTTC 4 cut(s) 17, 86, 415, 563
BstBAI YACGTR 1 cut(s) 738
BstC8I GCNNGC 1 cut(s) 471
BstDEI CTNAG 5 cut(s) 280, 344, 382, 461, 529
BstF5I GGATG 2 cut(s) 657, 707
BstKTI GATC 3 cut(s) 169, 337, 403
BstMBI GATC 3 cut(s) 166, 334, 400
BstMWI GCNNNNNNNGC 1 cut(s) 299
BstNI CCWGG 2 cut(s) 171, 756
BstSCI CCNGG 2 cut(s) 169, 754
BstSFI CTRYAG 1 cut(s) 195
BstV1I GCAGC 1 cut(s) 560
BstV2I GAAGAC 2 cut(s) 341, 821
BstX2I RGATCY 2 cut(s) 166, 334
BstYI RGATCY 2 cut(s) 166, 334
Bsu36I CCTNAGG 1 cut(s) 382
BsuRI GGCC 2 cut(s) 247, 578
BtsCI GGATG 2 cut(s) 657, 707
Cac8I GCNNGC 1 cut(s) 471
Cfr13I GGNCC 1 cut(s) 577
Csp6I GTAC 2 cut(s) 20, 458
CviQI GTAC 2 cut(s) 20, 458
DdeI CTNAG 5 cut(s) 280, 344, 382, 461, 529
DpnI GATC 3 cut(s) 168, 336, 402
DpnII GATC 3 cut(s) 166, 334, 400
Eam1104I CTCTTC 4 cut(s) 17, 86, 415, 563
EarI CTCTTC 4 cut(s) 17, 86, 415, 563
Eco147I AGGCCT 1 cut(s) 247
Eco32I GATATC 1 cut(s) 634
Eco81I CCTNAGG 1 cut(s) 382
EcoRII CCWGG 2 cut(s) 169, 754
EcoRV GATATC 1 cut(s) 634
EcoT22I ATGCAT 2 cut(s) 94, 785
FalI AAGNNNNNCTT 4 cut(s) 345, 377, 603, 635
FaqI GGGAC 2 cut(s) 242, 260
FauI CCCGC 1 cut(s) 222
FauNDI CATATG 2 cut(s) 220, 820
FblI GTMKAC 1 cut(s) 624
Fnu4HI GCNGC 1 cut(s) 549
FokI GGATG 2 cut(s) 644, 714
Fsp4HI GCNGC 1 cut(s) 549
FspBI CTAG 1 cut(s) 839
GluI GCNGC 1 cut(s) 549
GsuI CTGGAG 2 cut(s) 324, 738
HaeIII GGCC 2 cut(s) 247, 578
HindIII AAGCTT 2 cut(s) 569, 602
HinfI GANTC 5 cut(s) 130, 273, 296, 346, 770
HphI GGTGA 1 cut(s) 151
Hpy166II GTNNAC 1 cut(s) 625
Hpy188I TCNGA 6 cut(s) 30, 283, 345, 532, 811, 872
Hpy188III TCNNGA 5 cut(s) 96, 332, 353, 393, 608
Hpy8I GTNNAC 1 cut(s) 625
HpyAV CCTTC 2 cut(s) 249, 601
HpyCH4III ACNGT 1 cut(s) 199
HpyCH4IV ACGT 1 cut(s) 737
HpyCH4V TGCA 3 cut(s) 92, 783, 824
HpyF10VI GCNNNNNNNGC 1 cut(s) 299
HpyF3I CTNAG 5 cut(s) 280, 344, 382, 461, 529
HpySE526I ACGT 1 cut(s) 737
Kzo9I GATC 3 cut(s) 166, 334, 400
LmnI GCTCC 1 cut(s) 440
Lsp1109I GCAGC 1 cut(s) 560
LweI GCATC 2 cut(s) 101, 577
MaeI CTAG 1 cut(s) 839
MaeII ACGT 1 cut(s) 737
MalI GATC 3 cut(s) 168, 336, 402
MboI GATC 3 cut(s) 166, 334, 400
MflI RGATCY 2 cut(s) 166, 334
MhlI GDGCHC 1 cut(s) 530
MluCI AATT 4 cut(s) 37, 154, 450, 863
MlyI GAGTC 2 cut(s) 282, 355
Mph1103I ATGCAT 2 cut(s) 94, 785
MseI TTAA 1 cut(s) 831
MspA1I CMGCKG 2 cut(s) 137, 302
MspR9I CCNGG 2 cut(s) 171, 756
MvaI CCWGG 2 cut(s) 171, 756
MwoI GCNNNNNNNGC 1 cut(s) 299
NdeI CATATG 2 cut(s) 220, 820
NdeII GATC 3 cut(s) 166, 334, 400
NlaIV GGNNCC 3 cut(s) 168, 336, 760
NsiI ATGCAT 2 cut(s) 94, 785
PceI AGGCCT 1 cut(s) 247
PfeI GAWTC 3 cut(s) 130, 296, 770
PflMI CCANNNNNTGG 1 cut(s) 421
PfoI TCCNGGA 2 cut(s) 169, 754
PkrI GCNGC 1 cut(s) 550
PleI GAGTC 2 cut(s) 281, 354
PpsI GAGTC 2 cut(s) 281, 354
Ppu21I YACGTR 1 cut(s) 738
Psp6I CCWGG 2 cut(s) 169, 754
PspGI CCWGG 2 cut(s) 169, 754
PspN4I GGNNCC 3 cut(s) 168, 336, 760
PspPI GGNCC 1 cut(s) 577
PsuI RGATCY 2 cut(s) 166, 334
PvuII CAGCTG 1 cut(s) 302
RsaI GTAC 2 cut(s) 21, 459
RsaNI GTAC 2 cut(s) 20, 458
SaqAI TTAA 1 cut(s) 831
SatI GCNGC 1 cut(s) 549
Sau3AI GATC 3 cut(s) 166, 334, 400
Sau96I GGNCC 1 cut(s) 577
ScaI AGTACT 2 cut(s) 21, 459
SchI GAGTC 2 cut(s) 282, 355
ScrFI CCNGG 2 cut(s) 171, 756
SduI GDGCHC 1 cut(s) 530
SfaNI GCATC 2 cut(s) 101, 577
SfcI CTRYAG 1 cut(s) 195
Sse9I AATT 4 cut(s) 37, 154, 450, 863
SseBI AGGCCT 1 cut(s) 247
SsiI CCGC 2 cut(s) 137, 215
SspI AATATT 1 cut(s) 721
SspMI CTAG 1 cut(s) 839
StuI AGGCCT 1 cut(s) 247
StyD4I CCNGG 2 cut(s) 169, 754
TaaI ACNGT 1 cut(s) 199
TaiI ACGT 1 cut(s) 740
TaqI TCGA 2 cut(s) 566, 846
TaqII GACCGA 1 cut(s) 34
TasI AATT 4 cut(s) 37, 154, 450, 863
TatI WGTACW 2 cut(s) 19, 457
TfiI GAWTC 3 cut(s) 130, 296, 770
Tru1I TTAA 1 cut(s) 831
Tru9I TTAA 1 cut(s) 831
TseI GCWGC 1 cut(s) 548
TspDTI ATGAA 3 cut(s) 14, 30, 223
TspGWI ACGGA 1 cut(s) 256
Van91I CCANNNNNTGG 1 cut(s) 421
XapI RAATTY 1 cut(s) 863
XcmI CCANNNNNNNNNTGG 1 cut(s) 92
XmiI GTMKAC 1 cut(s) 624
XspI CTAG 1 cut(s) 839
ZrmI AGTACT 2 cut(s) 21, 459
Zsp2I ATGCAT 2 cut(s) 94, 785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.