Rroxscaffold_2G00143660

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000393
Physical Location & Seq
Forward (+)
81645701 .. 81647404
1704 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143660.1

Sequence Viewer

Length: 969 bp
ATGGCTTTTCTCTCCCAAATACTTGTTCCTTCATCTTCATCTGTCAAAATTTTCTCAATCATTGCAGTCATTTTCCTCTCCCATAATGTTGCTGCAGTAACACCAGCCACAAATAAAACAAATAGAGCAGTTTTTACTTTTGGAGATTCAACAATGGACACGGGCAATAATGACTACATCATTTCTATAGCCAAAAGCAATTTCCCACCTTATGGGAGAGACTTTGTGGGAGGAAAGCCTACTGGAAGATTTAGCAATGGAAAAGTCCCCTCAGATTTTGTTGCTGAAATATTTGGAGTGAAGAAGATTTTGCCAGCTTATCTGGATCCGAAATTGCAGCTTCAAGACCTACTTACTGGTGTATGTTTTGCCTCAGCAGGATCAGGATATGACCCTCTCACTGCCAAATTACTTTCTGTCTTGTCACTACCAGATCAGTTAGACTTGTTTAAAAAATACAAAAGCAAGATAACTGCAGCAGTTGGGACTGAAAGAACAGCCACTATACTGTCGAAAACCATTTCCTTTCTTTCCATGGGAAGCAATGACCTTGCAATTAATTATTTTTCTTCACCACTGAGGAGCCCTCACTATGACATTCCAGCTTATACAGATCTCATGATTGAATCAGCTTCAAATTTCTTGCAGGAACTTTATGCACTGGGAGCAAGAGTGATCGGAGTAGTATGTATACCATCAATTGGGTGTTTGCCAGCACAGAGAACACTTAATGGAGGTATAAAGAGAGCGTGCTATGAAACTGGGAATCAAGCAGCAATCCTTTTCAACTCCAAGCTCTCCCACCTTATAGATTCCCTCAATGAGAGACTTCCAGAAGCACACCTCATGTACATTGATAACTACAGCCCTTTGCTTTCCCTGATCCAAAACCCTGCTCAATATGGATTTGAAGTGGTGGAAAAAGGATGCTGTGGAACAGGAGATCTTGAATTTTGGCCCTCTGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

322

Amino Acids

34.87

Weight (kDa)

6.89

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 315 1.3e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 212, 701
AccI GTMKAC 1 cut(s) 691
AclWI GGATC 4 cut(s) 320, 333, 388, 877
AcsI RAATTY 3 cut(s) 48, 637, 950
AfaI GTAC 1 cut(s) 851
AfiI CCNNNNNNNGG 2 cut(s) 212, 701
AgsI TTSAA 7 cut(s) 150, 344, 626, 636, 787, 911, 950
AjuI GAANNNNNNNTTGG 4 cut(s) 9, 41, 185, 217
AluBI AGCT 5 cut(s) 317, 340, 605, 632, 796
AluI AGCT 5 cut(s) 317, 340, 605, 632, 796
Alw26I GTCTC 2 cut(s) 213, 820
AlwI GGATC 4 cut(s) 320, 333, 388, 877
AoxI GGCC 1 cut(s) 956
ApeKI GCWGC 4 cut(s) 92, 337, 476, 773
ApoI RAATTY 3 cut(s) 48, 637, 950
AseI ATTAAT 1 cut(s) 558
AspS9I GGNCC 1 cut(s) 957
AsuHPI GGTGA 1 cut(s) 564
BamHI GGATCC 1 cut(s) 325
BanII GRGCYC 1 cut(s) 587
BbvCI CCTCAGC 1 cut(s) 373
BbvI GCAGC 4 cut(s) 79, 349, 488, 785
BccI CCATC 1 cut(s) 703
BcoDI GTCTC 2 cut(s) 213, 820
BfmI CTRYAG 4 cut(s) 93, 186, 474, 862
BglII AGATCT 2 cut(s) 613, 943
BisI GCNGC 4 cut(s) 93, 338, 477, 774
BlsI GCNGC 4 cut(s) 94, 339, 478, 775
BmgT120I GGNCC 1 cut(s) 957
BmiI GGNNCC 2 cut(s) 327, 584
BmrI ACTGGG 2 cut(s) 671, 771
BmsI GCATC 1 cut(s) 917
BmuI ACTGGG 2 cut(s) 671, 771
BplI GAGNNNNNCTC 2 cut(s) 571, 603
Bpu10I CCTNAGC 1 cut(s) 373
BsaJI CCNNGG 1 cut(s) 534
BsaXI ACNNNNNCTCC 2 cut(s) 657, 687
Bsc4I CCNNNNNNNGG 2 cut(s) 212, 701
Bse1I ACTGG 4 cut(s) 247, 361, 666, 766
Bse3DI GCAATG 3 cut(s) 60, 262, 550
BseDI CCNNGG 1 cut(s) 534
BseGI GGATG 1 cut(s) 932
BseLI CCNNNNNNNGG 2 cut(s) 212, 701
BseMI GCAATG 3 cut(s) 60, 262, 550
BseMII CTCAG 3 cut(s) 285, 387, 569
BseNI ACTGG 4 cut(s) 247, 361, 666, 766
BseRI GAGGAG 1 cut(s) 595
BseXI GCAGC 4 cut(s) 79, 349, 488, 785
BshFI GGCC 1 cut(s) 958
BslFI GGGAC 2 cut(s) 251, 499
BslI CCNNNNNNNGG 2 cut(s) 212, 701
BsmAI GTCTC 2 cut(s) 213, 820
BsmFI GGGAC 2 cut(s) 251, 499
BsnI GGCC 1 cut(s) 958
Bsp1286I GDGCHC 1 cut(s) 587
Bsp1407I TGTACA 1 cut(s) 849
Bsp143I GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
Bsp19I CCATGG 1 cut(s) 534
BspANI GGCC 1 cut(s) 958
BspCNI CTCAG 3 cut(s) 284, 386, 570
BspHI TCATGA 1 cut(s) 618
BspLI GGNNCC 2 cut(s) 327, 584
BspMAI CTGCAG 2 cut(s) 97, 478
BspPI GGATC 4 cut(s) 320, 333, 388, 877
BsrDI GCAATG 3 cut(s) 60, 262, 550
BsrGI TGTACA 1 cut(s) 849
BsrI ACTGG 4 cut(s) 247, 361, 666, 766
BssECI CCNNGG 1 cut(s) 534
BssMI GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
BssNAI GTATAC 1 cut(s) 692
BssT1I CCWWGG 1 cut(s) 534
Bst1107I GTATAC 1 cut(s) 692
Bst4CI ACNGT 1 cut(s) 510
BstAUI TGTACA 1 cut(s) 849
BstC8I GCNNGC 3 cut(s) 315, 714, 751
BstDEI CTNAG 3 cut(s) 271, 373, 578
BstDSI CCRYGG 1 cut(s) 534
BstF5I GGATG 1 cut(s) 932
BstKTI GATC 7 cut(s) 328, 383, 436, 616, 678, 885, 946
BstMAI GTCTC 2 cut(s) 213, 820
BstMBI GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstSFI CTRYAG 4 cut(s) 93, 186, 474, 862
BstV1I GCAGC 4 cut(s) 79, 349, 488, 785
BstX2I RGATCY 3 cut(s) 325, 613, 943
BstYI RGATCY 3 cut(s) 325, 613, 943
BstZ17I GTATAC 1 cut(s) 692
BsuRI GGCC 1 cut(s) 958
BtgI CCRYGG 1 cut(s) 534
BtsCI GGATG 1 cut(s) 932
BtsI GCAGTG 1 cut(s) 399
BtsIMutI CAGTG 3 cut(s) 399, 575, 659
Cac8I GCNNGC 3 cut(s) 315, 714, 751
CciI TCATGA 1 cut(s) 618
Cfr13I GGNCC 1 cut(s) 957
Csp6I GTAC 1 cut(s) 850
CviAII CATG 3 cut(s) 535, 619, 847
CviQI GTAC 1 cut(s) 850
DdeI CTNAG 3 cut(s) 271, 373, 578
DpnI GATC 7 cut(s) 327, 382, 435, 615, 677, 884, 945
DpnII GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
DraI TTTAAA 1 cut(s) 451
Eco130I CCWWGG 1 cut(s) 534
Eco24I GRGCYC 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 534
EcoT38I GRGCYC 1 cut(s) 587
ErhI CCWWGG 1 cut(s) 534
FaeI CATG 3 cut(s) 538, 622, 850
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FaqI GGGAC 2 cut(s) 251, 499
FatI CATG 3 cut(s) 534, 618, 846
FblI GTMKAC 1 cut(s) 691
Fnu4HI GCNGC 4 cut(s) 93, 338, 477, 774
FokI GGATG 1 cut(s) 939
FriOI GRGCYC 1 cut(s) 587
Fsp4HI GCNGC 4 cut(s) 93, 338, 477, 774
GluI GCNGC 4 cut(s) 93, 338, 477, 774
HaeIII GGCC 1 cut(s) 958
Hin1II CATG 3 cut(s) 538, 622, 850
HinfI GANTC 4 cut(s) 146, 626, 766, 812
HphI GGTGA 1 cut(s) 564
Hpy166II GTNNAC 1 cut(s) 692
Hpy188I TCNGA 3 cut(s) 274, 330, 680
Hpy188III TCNNGA 6 cut(s) 323, 344, 384, 619, 833, 947
Hpy8I GTNNAC 1 cut(s) 692
HpyAV CCTTC 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 510
HpyCH4V TGCA 7 cut(s) 65, 95, 337, 476, 554, 646, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 3 cut(s) 271, 373, 578
Hsp92II CATG 3 cut(s) 538, 622, 850
Kzo9I GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
LmnI GCTCC 2 cut(s) 582, 665
Lsp1109I GCAGC 4 cut(s) 79, 349, 488, 785
LweI GCATC 1 cut(s) 917
MaeIII GTNAC 2 cut(s) 97, 423
MalI GATC 7 cut(s) 327, 382, 435, 615, 677, 884, 945
MboI GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
MboII GAAGA 5 cut(s) 27, 258, 313, 316, 561
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 3 cut(s) 325, 613, 943
MhlI GDGCHC 1 cut(s) 587
MluCI AATT 9 cut(s) 48, 199, 332, 407, 555, 559, 637, 699, 950
MseI TTAA 3 cut(s) 450, 558, 729
MunI CAATTG 1 cut(s) 699
MwoI GCNNNNNNNGC 1 cut(s) 665
NcoI CCATGG 1 cut(s) 534
NdeII GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
NlaIII CATG 3 cut(s) 538, 622, 850
NlaIV GGNNCC 2 cut(s) 327, 584
NmuCI GTSAC 1 cut(s) 423
PagI TCATGA 1 cut(s) 618
PfeI GAWTC 4 cut(s) 146, 626, 766, 812
PflMI CCANNNNNTGG 2 cut(s) 212, 701
PkrI GCNGC 4 cut(s) 94, 339, 478, 775
PshBI ATTAAT 1 cut(s) 558
PspN4I GGNNCC 2 cut(s) 327, 584
PspPI GGNCC 1 cut(s) 957
PstI CTGCAG 2 cut(s) 97, 478
PsuI RGATCY 3 cut(s) 325, 613, 943
RsaI GTAC 1 cut(s) 851
RsaNI GTAC 1 cut(s) 850
SaqAI TTAA 3 cut(s) 450, 558, 729
SatI GCNGC 4 cut(s) 93, 338, 477, 774
Sau3AI GATC 7 cut(s) 325, 380, 433, 613, 675, 882, 943
Sau96I GGNCC 1 cut(s) 957
SduI GDGCHC 1 cut(s) 587
SfaNI GCATC 1 cut(s) 917
SfcI CTRYAG 4 cut(s) 93, 186, 474, 862
Sse9I AATT 9 cut(s) 48, 199, 332, 407, 555, 559, 637, 699, 950
SspI AATATT 1 cut(s) 291
StyI CCWWGG 1 cut(s) 534
TaaI ACNGT 1 cut(s) 510
TaqI TCGA 1 cut(s) 512
TasI AATT 9 cut(s) 48, 199, 332, 407, 555, 559, 637, 699, 950
TatI WGTACW 1 cut(s) 849
TfiI GAWTC 4 cut(s) 146, 626, 766, 812
Tru1I TTAA 3 cut(s) 450, 558, 729
Tru9I TTAA 3 cut(s) 450, 558, 729
TscAI CASTG 3 cut(s) 406, 582, 666
TseFI GTSAC 1 cut(s) 423
TseI GCWGC 4 cut(s) 92, 337, 476, 773
Tsp45I GTSAC 1 cut(s) 423
TspDTI ATGAA 3 cut(s) 21, 27, 771
TspRI CASTG 3 cut(s) 406, 582, 666
Van91I CCANNNNNTGG 2 cut(s) 212, 701
VspI ATTAAT 1 cut(s) 558
XapI RAATTY 3 cut(s) 48, 637, 950
XmiI GTMKAC 1 cut(s) 691
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.