Rroxscaffold_2G00143680

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000393
Physical Location & Seq
Forward (+)
81653256 .. 81653697
442 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00143680.1

Sequence Viewer

Length: 369 bp
ATGCCGCCAATTGGGTGTGCGCCAGCACAGGGAACACTCAATGGAGGTACAAACAGAGCCTGCTATGAGACTGAGAACCAAGCAGCAATCCTCTTCGACACAAAGCTCTTCCCCCGCATAGATTCCCTCAATAAGAGACTTCAAGAAGCAAAACTCATTTACATTGATATATATAACCCTCTGCTTTCGATGATCCAAGACCCTGCTCAATATGGATTTGAAGTGGTAGATAAAGGATGCTGTGGAACAGAAACCATTAAGCTCGGGCCTCTGTGCAACAAATACACTCCAGGTATTGTTCTGGGATGGCATTCATCCCACAGAAACAGCTTACAGGATCCTGACCTCAGAAATATTAAAGCAAGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

122

Amino Acids

13.47

Weight (kDa)

6.7

Isoelectric Point (pI)

43.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 1 - 84 8.4e-06 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 5, 115
AclWI GGATC 3 cut(s) 187, 332, 345
AfaI GTAC 1 cut(s) 49
AfiI CCNNNNNNNGG 2 cut(s) 11, 29
AgsI TTSAA 2 cut(s) 143, 221
AjnI CCWGG 1 cut(s) 289
AluBI AGCT 3 cut(s) 106, 262, 330
AluI AGCT 3 cut(s) 106, 262, 330
Alw26I GTCTC 2 cut(s) 62, 130
AlwI GGATC 3 cut(s) 187, 332, 345
AlwNI CAGNNNCTG 1 cut(s) 60
Ama87I CYCGRG 1 cut(s) 263
AoxI GGCC 1 cut(s) 266
ApeKI GCWGC 1 cut(s) 83
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 263
BamHI GGATCC 1 cut(s) 337
BbvI GCAGC 1 cut(s) 95
BccI CCATC 1 cut(s) 300
BciT130I CCWGG 1 cut(s) 291
BcoDI GTCTC 2 cut(s) 62, 130
BisI GCNGC 2 cut(s) 5, 84
BlsI GCNGC 2 cut(s) 6, 85
Bme1390I CCNGG 1 cut(s) 291
BmeT110I CYCGRG 1 cut(s) 263
BmgT120I GGNCC 1 cut(s) 266
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 291
BmsI GCATC 1 cut(s) 227
BpmI CTGGAG 1 cut(s) 273
Bsc4I CCNNNNNNNGG 2 cut(s) 11, 29
BseBI CCWGG 1 cut(s) 291
BseGI GGATG 3 cut(s) 242, 311, 314
BseLI CCNNNNNNNGG 2 cut(s) 11, 29
BseMII CTCAG 2 cut(s) 63, 361
BseXI GCAGC 1 cut(s) 95
BshFI GGCC 1 cut(s) 268
BsiHKCI CYCGRG 1 cut(s) 263
BslI CCNNNNNNNGG 2 cut(s) 11, 29
BsmAI GTCTC 2 cut(s) 62, 130
BsmI GAATGC 1 cut(s) 310
BsnI GGCC 1 cut(s) 268
BsoBI CYCGRG 1 cut(s) 263
Bsp143I GATC 2 cut(s) 192, 337
BspACI CCGC 2 cut(s) 5, 115
BspANI GGCC 1 cut(s) 268
BspCNI CTCAG 2 cut(s) 64, 360
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 3 cut(s) 187, 332, 345
BspQI GCTCTTC 1 cut(s) 113
BssMI GATC 2 cut(s) 192, 337
Bst2UI CCWGG 1 cut(s) 291
Bst6I CTCTTC 2 cut(s) 98, 113
BstC8I GCNNGC 2 cut(s) 24, 61
BstDEI CTNAG 2 cut(s) 72, 347
BstF5I GGATG 3 cut(s) 242, 311, 314
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 2 cut(s) 195, 340
BstMAI GTCTC 2 cut(s) 62, 130
BstMBI GATC 2 cut(s) 192, 337
BstNI CCWGG 1 cut(s) 291
BstSCI CCNGG 1 cut(s) 289
BstV1I GCAGC 1 cut(s) 95
BstX2I RGATCY 1 cut(s) 337
BstYI RGATCY 1 cut(s) 337
BsuRI GGCC 1 cut(s) 268
BtsCI GGATG 3 cut(s) 242, 311, 314
Cac8I GCNNGC 2 cut(s) 24, 61
CaiI CAGNNNCTG 1 cut(s) 60
CfoI GCGC 1 cut(s) 22
Cfr13I GGNCC 1 cut(s) 266
Csp6I GTAC 1 cut(s) 48
CviJI RGCY 5 cut(s) 59, 106, 262, 268, 330
CviKI_1 RGCY 5 cut(s) 59, 106, 262, 268, 330
CviQI GTAC 1 cut(s) 48
DdeI CTNAG 2 cut(s) 72, 347
DpnI GATC 2 cut(s) 194, 339
DpnII GATC 2 cut(s) 192, 337
Eam1104I CTCTTC 2 cut(s) 98, 113
EarI CTCTTC 2 cut(s) 98, 113
Eco88I CYCGRG 1 cut(s) 263
EcoRII CCWGG 1 cut(s) 289
FaiI YATR 6 cut(s) 66, 119, 170, 172, 174, 213
FauI CCCGC 1 cut(s) 122
Fnu4HI GCNGC 2 cut(s) 5, 84
FokI GGATG 3 cut(s) 249, 301, 318
Fsp4HI GCNGC 2 cut(s) 5, 84
GlaI GCGC 1 cut(s) 21
GluI GCNGC 2 cut(s) 5, 84
GsuI CTGGAG 1 cut(s) 273
HaeIII GGCC 1 cut(s) 268
HhaI GCGC 1 cut(s) 22
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HinfI GANTC 1 cut(s) 122
Hpy188I TCNGA 1 cut(s) 350
Hpy188III TCNNGA 2 cut(s) 143, 341
HpyCH4V TGCA 1 cut(s) 276
HpyF3I CTNAG 2 cut(s) 72, 347
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 2 cut(s) 192, 337
LguI GCTCTTC 1 cut(s) 113
LpnPI CCDG 9 cut(s) 14, 36, 73, 216, 276, 287, 303, 320, 354
Lsp1109I GCAGC 1 cut(s) 95
LweI GCATC 1 cut(s) 227
MalI GATC 2 cut(s) 194, 339
MboI GATC 2 cut(s) 192, 337
MboII GAAGA 2 cut(s) 85, 100
MfeI CAATTG 1 cut(s) 9
MflI RGATCY 1 cut(s) 337
MluCI AATT 1 cut(s) 9
MnlI CCTC 6 cut(s) 38, 101, 137, 189, 279, 356
MseI TTAA 3 cut(s) 258, 357, 367
MspR9I CCNGG 1 cut(s) 291
MunI CAATTG 1 cut(s) 9
Mva1269I GAATGC 1 cut(s) 310
MvaI CCWGG 1 cut(s) 291
NdeII GATC 2 cut(s) 192, 337
NlaIV GGNNCC 1 cut(s) 339
PciSI GCTCTTC 1 cut(s) 113
PctI GAATGC 1 cut(s) 310
PfeI GAWTC 1 cut(s) 122
PkrI GCNGC 2 cut(s) 6, 85
Psp6I CCWGG 1 cut(s) 289
PspGI CCWGG 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 1 cut(s) 266
PstNI CAGNNNCTG 1 cut(s) 60
PsuI RGATCY 1 cut(s) 337
RsaI GTAC 1 cut(s) 49
RsaNI GTAC 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 113
SaqAI TTAA 3 cut(s) 258, 357, 367
SatI GCNGC 2 cut(s) 5, 84
Sau3AI GATC 2 cut(s) 192, 337
Sau96I GGNCC 1 cut(s) 266
ScrFI CCNGG 1 cut(s) 291
SetI ASST 6 cut(s) 49, 108, 264, 295, 332, 348
SfaNI GCATC 1 cut(s) 227
Sse9I AATT 1 cut(s) 9
SsiI CCGC 2 cut(s) 5, 115
SspI AATATT 1 cut(s) 355
StyD4I CCNGG 1 cut(s) 289
TaqI TCGA 2 cut(s) 96, 188
TasI AATT 1 cut(s) 9
TauI GCSGC 1 cut(s) 7
TfiI GAWTC 1 cut(s) 122
Tru1I TTAA 3 cut(s) 258, 357, 367
Tru9I TTAA 3 cut(s) 258, 357, 367
TseI GCWGC 1 cut(s) 83
TspDTI ATGAA 1 cut(s) 303
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.