FvH4_5g34670

hydrolase activity, acting on ester bonds

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000393
Physical Location & Seq
Forward (+)
25394260 .. 25396310
2051 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g34670.t1

Sequence Viewer

Length: 1125 bp
ATGCTGAGGCACTACTTTGAAATTCTGCTTTCAGTAGTAGTACTATCTGTTCAACTCCAAGTTATAATAATATGTCTGTGCACCTCAAACATTAGTTATCGTACTATAGTAGTAGCAGAACAGGAAGGCCGACCAACTGAAATCGACGGAATGGCGTCGCCGTTTGTAGGGGTTTTCGCTTTCGGAGATTCAGTTCTTGATACTGGCAACAATAATAACCTCTCTAGTGCAGCCAAATGCAATTTTCCACCCTATGGAAGAGATTTCATCGGAGGCATCCCAACCGGAAGATTTAGCAATGGGAAAATTTACCCTGATTTTATAGTGGAAGCCTTGGGTATCAAGCATGTCTTGCCAGCCTATCTAGACCCAAAGCTTCGCTCTGAAGATCTCCCAACTGGTGTATGCTTTGCTTCTGGTAGTTCTGGTTATGATCCTCTAACAGCATCTACAGTGGGAGTTTTAACTTTGTCAGATCAATTAGAACTCTTCAAGGACTACAGAGTGAAACTAATCGGATTAGTTGGCGAATCAATCGCAAAAACCATCATAGCCAACAGTTTGTTTTTAGTTTCATCAGGCAACAATGATATTATTATTACATATCCTCTCCTTGCCCGAAAGGATGAGTTTCACTCATATGCTGCGCTTCTGGTTAGCTTCGCTTCAACATTCTTAAAGGATTTATATAGCATTGGGGCACGGCGAATCGGTATTCTAAATGTAGCCCCATTGGGATGCCTACCGTTGGAAAGAAATGGAGGAAGACTAGAAAGAGTATGTTTGGAGTTGCAAAACCAAAGAGCGAAGATGTTCAATTCCCAGTTATCATCCGAATTAGATACCATCAACAAGGACTTCCCAGATGCCAAGCTAGTTTTACTTGATATCTACCACCCTCTTCTTGCACTCAACCAACACCCTCAAAACTCAGGATTTGAAGTGGTGAAGTTCGGGTGCCGTGGCAGTGGAACGGTGGAAGTGACGTCGTTTTTATGCAATCCATTGAGTACATCTACATGTTCAAATGCATCTAAATACGTATACTGGGATGCACTTCATACCACCGAGCGAGCTCACAGATTGATCGCTTCTCAATCTTTCAAATTGATCACCAGTATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

375

Amino Acids

40.93

Weight (kDa)

6.39

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 58 - 365 1.1e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 65
AatII GACGTC 1 cut(s) 989
AccB1I GGYRCC 1 cut(s) 957
AccB7I CCANNNNNTGG 1 cut(s) 254
AccI GTMKAC 1 cut(s) 1044
AclWI GGATC 1 cut(s) 428
AcsI RAATTY 2 cut(s) 21, 306
AcuI CTGAAG 1 cut(s) 405
AcyI GRCGYC 2 cut(s) 155, 986
AfaI GTAC 3 cut(s) 42, 103, 1012
AfiI CCNNNNNNNGG 3 cut(s) 167, 254, 748
AflIII ACRYGT 1 cut(s) 1019
AgsI TTSAA 8 cut(s) 20, 53, 493, 669, 817, 941, 1026, 1105
AloI GAACNNNNNNTCC 2 cut(s) 177, 209
AluBI AGCT 4 cut(s) 376, 660, 874, 1076
AluI AGCT 4 cut(s) 376, 660, 874, 1076
Alw21I GWGCWC 2 cut(s) 83, 1078
Alw44I GTGCAC 1 cut(s) 79
AlwI GGATC 1 cut(s) 428
AoxI GGCC 1 cut(s) 127
ApaLI GTGCAC 1 cut(s) 79
ApeKI GCWGC 2 cut(s) 230, 644
ApoI RAATTY 2 cut(s) 21, 306
Asp700I GAANNNNTTC 1 cut(s) 812
AspLEI GCGC 1 cut(s) 649
AsuHPI GGTGA 2 cut(s) 958, 1105
BaeGI GKGCMC 2 cut(s) 83, 703
BanI GGYRCC 1 cut(s) 957
BanII GRGCYC 1 cut(s) 1078
BbsI GAAGAC 1 cut(s) 772
Bbv12I GWGCWC 2 cut(s) 83, 1078
BbvCI CCTCAGC 1 cut(s) 5
BbvI GCAGC 2 cut(s) 242, 631
BccI CCATC 2 cut(s) 554, 854
BceAI ACGGC 3 cut(s) 145, 719, 945
BclI TGATCA 1 cut(s) 1110
BfaI CTAG 4 cut(s) 225, 365, 770, 875
BfmI CTRYAG 3 cut(s) 105, 450, 499
BglII AGATCT 1 cut(s) 388
BisI GCNGC 2 cut(s) 231, 645
BlsI GCNGC 2 cut(s) 232, 646
BmcAI AGTACT 1 cut(s) 42
BmiI GGNNCC 1 cut(s) 959
BmrI ACTGGG 2 cut(s) 817, 1057
BmsI GCATC 6 cut(s) 285, 455, 728, 856, 1040, 1042
BmuI ACTGGG 2 cut(s) 817, 1057
BpiI GAAGAC 1 cut(s) 772
BplI GAGNNNNNCTC 2 cut(s) 620, 652
Bpu10I CCTNAGC 1 cut(s) 5
BsaAI YACGTR 1 cut(s) 1042
BsaHI GRCGYC 2 cut(s) 155, 986
BsaJI CCNNGG 2 cut(s) 333, 961
BsaWI WCCGGW 1 cut(s) 284
BsaXI ACNNNNNCTCC 2 cut(s) 177, 207
Bsc4I CCNNNNNNNGG 3 cut(s) 167, 254, 748
Bse1I ACTGG 5 cut(s) 208, 403, 823, 1052, 1116
Bse3DI GCAATG 1 cut(s) 304
BseDI CCNNGG 2 cut(s) 333, 961
BseGI GGATG 5 cut(s) 276, 631, 743, 830, 1057
BseLI CCNNNNNNNGG 3 cut(s) 167, 254, 748
BseMI GCAATG 1 cut(s) 304
BseMII CTCAG 1 cut(s) 945
BseNI ACTGG 5 cut(s) 208, 403, 823, 1052, 1116
BseSI GKGCMC 2 cut(s) 83, 703
BseXI GCAGC 2 cut(s) 242, 631
BsgI GTGCAG 1 cut(s) 249
BshFI GGCC 1 cut(s) 129
BshNI GGYRCC 1 cut(s) 957
BsiHKAI GWGCWC 2 cut(s) 83, 1078
BsiSI CCGG 1 cut(s) 285
BslI CCNNNNNNNGG 3 cut(s) 167, 254, 748
BsnI GGCC 1 cut(s) 129
Bsp1286I GDGCHC 3 cut(s) 83, 703, 1078
Bsp143I GATC 5 cut(s) 388, 433, 475, 1086, 1110
BspANI GGCC 1 cut(s) 129
BspCNI CTCAG 1 cut(s) 944
BspLI GGNNCC 1 cut(s) 959
BspPI GGATC 1 cut(s) 428
BspT107I GGYRCC 1 cut(s) 957
BsrDI GCAATG 1 cut(s) 304
BsrI ACTGG 5 cut(s) 208, 403, 823, 1052, 1116
BssECI CCNNGG 2 cut(s) 333, 961
BssMI GATC 5 cut(s) 388, 433, 475, 1086, 1110
BssNAI GTATAC 1 cut(s) 1045
BssNI GRCGYC 2 cut(s) 155, 986
BssT1I CCWWGG 1 cut(s) 333
Bst1107I GTATAC 1 cut(s) 1045
Bst4CI ACNGT 4 cut(s) 454, 560, 747, 976
Bst6I CTCTTC 3 cut(s) 253, 494, 906
BstACI GRCGYC 2 cut(s) 155, 986
BstAPI GCANNNNNTGC 1 cut(s) 352
BstBAI YACGTR 1 cut(s) 1042
BstC8I GCNNGC 2 cut(s) 357, 1074
BstDEI CTNAG 2 cut(s) 5, 931
BstDSI CCRYGG 1 cut(s) 961
BstF5I GGATG 5 cut(s) 276, 631, 743, 830, 1057
BstHHI GCGC 1 cut(s) 649
BstKTI GATC 5 cut(s) 391, 436, 478, 1089, 1113
BstMBI GATC 5 cut(s) 388, 433, 475, 1086, 1110
BstMWI GCNNNNNNNGC 1 cut(s) 352
BstNSI RCATGY 2 cut(s) 350, 1023
BstSFI CTRYAG 3 cut(s) 105, 450, 499
BstSLI GKGCMC 2 cut(s) 83, 703
BstSNI TACGTA 1 cut(s) 1042
BstV1I GCAGC 2 cut(s) 242, 631
BstV2I GAAGAC 1 cut(s) 772
BstX2I RGATCY 1 cut(s) 388
BstYI RGATCY 1 cut(s) 388
BstZ17I GTATAC 1 cut(s) 1045
BsuRI GGCC 1 cut(s) 129
BtgI CCRYGG 1 cut(s) 961
BtsCI GGATG 5 cut(s) 276, 631, 743, 830, 1057
BtsI GCAGTG 1 cut(s) 973
BtsIMutI CAGTG 2 cut(s) 459, 973
Cac8I GCNNGC 2 cut(s) 357, 1074
CfoI GCGC 1 cut(s) 649
CseI GACGC 1 cut(s) 144
Csp6I GTAC 3 cut(s) 41, 102, 1011
CviAII CATG 2 cut(s) 347, 1020
CviQI GTAC 3 cut(s) 41, 102, 1011
DdeI CTNAG 2 cut(s) 5, 931
DpnI GATC 5 cut(s) 390, 435, 477, 1088, 1112
DpnII GATC 5 cut(s) 388, 433, 475, 1086, 1110
Eam1104I CTCTTC 3 cut(s) 253, 494, 906
EarI CTCTTC 3 cut(s) 253, 494, 906
Ecl136II GAGCTC 1 cut(s) 1076
Eco105I TACGTA 1 cut(s) 1042
Eco130I CCWWGG 1 cut(s) 333
Eco24I GRGCYC 1 cut(s) 1078
Eco32I GATATC 1 cut(s) 889
Eco53kI GAGCTC 1 cut(s) 1076
Eco57I CTGAAG 1 cut(s) 405
EcoICRI GAGCTC 1 cut(s) 1076
EcoRV GATATC 1 cut(s) 889
EcoT14I CCWWGG 1 cut(s) 333
EcoT22I ATGCAT 1 cut(s) 1033
EcoT38I GRGCYC 1 cut(s) 1078
ErhI CCWWGG 1 cut(s) 333
FaeI CATG 2 cut(s) 350, 1023
FalI AAGNNNNNCTT 2 cut(s) 335, 367
FatI CATG 2 cut(s) 346, 1019
FauNDI CATATG 1 cut(s) 640
FbaI TGATCA 1 cut(s) 1110
FblI GTMKAC 1 cut(s) 1044
Fnu4HI GCNGC 2 cut(s) 231, 645
FokI GGATG 5 cut(s) 263, 638, 750, 817, 1064
FriOI GRGCYC 1 cut(s) 1078
Fsp4HI GCNGC 2 cut(s) 231, 645
FspBI CTAG 4 cut(s) 225, 365, 770, 875
GlaI GCGC 1 cut(s) 648
GluI GCNGC 2 cut(s) 231, 645
HaeIII GGCC 1 cut(s) 129
HapII CCGG 1 cut(s) 285
HgaI GACGC 1 cut(s) 144
HhaI GCGC 1 cut(s) 649
Hin1I GRCGYC 2 cut(s) 155, 986
Hin1II CATG 2 cut(s) 350, 1023
Hin6I GCGC 1 cut(s) 647
HinP1I GCGC 1 cut(s) 647
HindIII AAGCTT 1 cut(s) 374
HinfI GANTC 3 cut(s) 188, 530, 708
HpaII CCGG 1 cut(s) 285
HphI GGTGA 2 cut(s) 958, 1105
Hpy166II GTNNAC 2 cut(s) 81, 1045
Hpy188I TCNGA 6 cut(s) 185, 272, 385, 475, 518, 835
Hpy188III TCNNGA 3 cut(s) 197, 365, 933
Hpy8I GTNNAC 2 cut(s) 81, 1045
Hpy99I CGWCG 3 cut(s) 149, 160, 991
HpyAV CCTTC 1 cut(s) 119
HpyCH4III ACNGT 4 cut(s) 454, 560, 747, 976
HpyCH4IV ACGT 2 cut(s) 986, 1041
HpyCH4V TGCA 8 cut(s) 81, 230, 240, 793, 908, 999, 1031, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 352
HpyF3I CTNAG 2 cut(s) 5, 931
HpySE526I ACGT 2 cut(s) 986, 1041
Hsp92I GRCGYC 2 cut(s) 155, 986
Hsp92II CATG 2 cut(s) 350, 1023
HspAI GCGC 1 cut(s) 647
Ksp22I TGATCA 1 cut(s) 1110
Kzo9I GATC 5 cut(s) 388, 433, 475, 1086, 1110
Lsp1109I GCAGC 2 cut(s) 242, 631
LweI GCATC 6 cut(s) 285, 455, 728, 856, 1040, 1042
MaeI CTAG 4 cut(s) 225, 365, 770, 875
MaeII ACGT 2 cut(s) 986, 1041
MaeIII GTNAC 1 cut(s) 982
MalI GATC 5 cut(s) 390, 435, 477, 1088, 1112
MboI GATC 5 cut(s) 388, 433, 475, 1086, 1110
MboII GAAGA 7 cut(s) 270, 300, 398, 481, 777, 820, 893
MflI RGATCY 1 cut(s) 388
MhlI GDGCHC 3 cut(s) 83, 703, 1078
MluCI AATT 7 cut(s) 21, 241, 306, 479, 817, 836, 1106
MmeI TCCRAC 1 cut(s) 729
MnlI CCTC 8 cut(s) 94, 230, 266, 447, 618, 755, 909, 933
Mph1103I ATGCAT 1 cut(s) 1033
MroXI GAANNNNTTC 1 cut(s) 812
MseI TTAA 2 cut(s) 464, 677
MslI CAYNNNNRTG 3 cut(s) 639, 736, 1018
MspI CCGG 1 cut(s) 285
MwoI GCNNNNNNNGC 1 cut(s) 352
NdeI CATATG 1 cut(s) 640
NdeII GATC 5 cut(s) 388, 433, 475, 1086, 1110
NlaIII CATG 2 cut(s) 350, 1023
NlaIV GGNNCC 1 cut(s) 959
NmuCI GTSAC 1 cut(s) 982
NsiI ATGCAT 1 cut(s) 1033
NspI RCATGY 2 cut(s) 350, 1023
PciI ACATGT 1 cut(s) 1019
PdmI GAANNNNTTC 1 cut(s) 812
PfeI GAWTC 3 cut(s) 188, 530, 708
PflMI CCANNNNNTGG 1 cut(s) 254
PkrI GCNGC 2 cut(s) 232, 646
Ppu21I YACGTR 1 cut(s) 1042
PscI ACATGT 1 cut(s) 1019
PsiI TTATAA 1 cut(s) 65
Psp124BI GAGCTC 1 cut(s) 1078
PspN4I GGNNCC 1 cut(s) 959
PsrI GAACNNNNNNTAC 2 cut(s) 33, 65
PsuI RGATCY 1 cut(s) 388
RsaI GTAC 3 cut(s) 42, 103, 1012
RsaNI GTAC 3 cut(s) 41, 102, 1011
RseI CAYNNNNRTG 3 cut(s) 639, 736, 1018
SacI GAGCTC 1 cut(s) 1078
SaqAI TTAA 2 cut(s) 464, 677
SatI GCNGC 2 cut(s) 231, 645
Sau3AI GATC 5 cut(s) 388, 433, 475, 1086, 1110
ScaI AGTACT 1 cut(s) 42
SduI GDGCHC 3 cut(s) 83, 703, 1078
SetI ASST 8 cut(s) 86, 222, 378, 662, 876, 989, 1044, 1078
SfaNI GCATC 6 cut(s) 285, 455, 728, 856, 1040, 1042
SfcI CTRYAG 3 cut(s) 105, 450, 499
SmiMI CAYNNNNRTG 3 cut(s) 639, 736, 1018
SnaBI TACGTA 1 cut(s) 1042
Sse9I AATT 7 cut(s) 21, 241, 306, 479, 817, 836, 1106
SspMI CTAG 4 cut(s) 225, 365, 770, 875
SstI GAGCTC 1 cut(s) 1078
StyI CCWWGG 1 cut(s) 333
TaaI ACNGT 4 cut(s) 454, 560, 747, 976
TaiI ACGT 2 cut(s) 989, 1044
TaqI TCGA 1 cut(s) 144
TasI AATT 7 cut(s) 21, 241, 306, 479, 817, 836, 1106
TatI WGTACW 2 cut(s) 40, 1010
TfiI GAWTC 3 cut(s) 188, 530, 708
Tru1I TTAA 2 cut(s) 464, 677
Tru9I TTAA 2 cut(s) 464, 677
TscAI CASTG 2 cut(s) 459, 973
TseFI GTSAC 1 cut(s) 982
TseI GCWGC 2 cut(s) 230, 644
Tsp45I GTSAC 1 cut(s) 982
TspDTI ATGAA 3 cut(s) 256, 564, 1049
TspGWI ACGGA 1 cut(s) 162
TspRI CASTG 2 cut(s) 459, 973
Van91I CCANNNNNTGG 1 cut(s) 254
VneI GTGCAC 1 cut(s) 79
XapI RAATTY 2 cut(s) 21, 306
XbaI TCTAGA 1 cut(s) 364
XceI RCATGY 2 cut(s) 350, 1023
XmiI GTMKAC 1 cut(s) 1044
XmnI GAANNNNTTC 1 cut(s) 812
XspI CTAG 4 cut(s) 225, 365, 770, 875
ZraI GACGTC 1 cut(s) 987
ZrmI AGTACT 1 cut(s) 42
Zsp2I ATGCAT 1 cut(s) 1033
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.