MD02G1127100.v1.1

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000393
Physical Location & Seq
Forward (+)
10283098 .. 10285207
2110 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1127100.v1.1.491

Sequence Viewer

Length: 1050 bp
ATGAACTTCCTCTCCCCAAAACTTCTTCCTTCATCATCTAAATCTTCTCTCGAACTTATAACGATCGCCATAGTCTTGTATGCTGCTGCTGTAGAGCTAGCGAACAACGAAACGATTCCGGCAGTGATTGTTTTTGGAGATTCAATAGTGGATCCGGGAAACAACAACAATATTGGCACGATAGTCAAATGCAATTTCCCACCTTATGGAAGGGACTTTGTAAGAAAAAGGCCTACCGGAAGGTTTAGCAATGGCAGAATTTTGCCAGCTTATTTGGACTCAAATCTGAAGATTCAAGACCTACTTACTGGTGTAAGTTTTGCCTCAGGTGGCTCAGGATATGATCCCCTCACTCCCAAAATAGTGTCTGTCTTATCTCTATCGGATCAACTGGATTTGTTCAAAAAATACATAAGCAAGATAAATGCAGTCGTTGGGAAGGAGAGAACAGCGACTATAGTATCGAAAATCATATACATTGTGTGCACAGGGAGTAACGACATTCCGAATACTTATTTATCTACACCACTAAGAAGATCTCACTATGACATTCCAGCGTATACCGATCTCATGGTTAAATCGGCTTCAAGTTTCTTTCAGGAACTATATGGACTCGGAGCAAGAAGAATTGGAGTTGTAAGTCTGCCAGCCATCGGGTGTGTACCGTCACAGAGAACACTGAGTGGAGGCTTAGACAGAGGGTGCTCGGAGACTGCCAACCAAGCAGCAAGTCTCTTCAACTCCAAGCTCTCCGCCCAAATAGATGCCTTCAATAGGAGGCTGCCAGAAGCAAGGCTTGTCTACCTCGATATCTATAACACATTGCTTTCCCTCATCCAAAACCCTACTCAATATGGATTTGAAGTGATCAATCGAGGATGTTGTGGGACGGGAAACTTTGAGGTTAGCATTCTGTGTACACGTTATTCTCCGGGAACCTGCAATGATTCGTCGAAATACATATTCTGGGACAGCTACCATCCTTCAGAGAAAGGATATGAAGTCATCACCTCTCTGGTTTTCGATTCGCAAGTTCATAAGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

350

Amino Acids

38.23

Weight (kDa)

8.67

Isoelectric Point (pI)

33.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 42 - 338 1.3e-33 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 947
AccB7I CCANNNNNTGG 1 cut(s) 206
AccI GTMKAC 2 cut(s) 560, 801
AciI CCGC 1 cut(s) 753
AclWI GGATC 4 cut(s) 146, 159, 338, 393
AcsI RAATTY 1 cut(s) 258
AcuI CTGAAG 2 cut(s) 308, 969
AdeI CACNNNGTG 1 cut(s) 683
AfaI GTAC 2 cut(s) 663, 919
AfiI CCNNNNNNNGG 3 cut(s) 206, 653, 774
AflIII ACRYGT 1 cut(s) 920
AgsI TTSAA 7 cut(s) 144, 296, 403, 588, 739, 772, 863
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 97, 269, 748, 975
AluI AGCT 4 cut(s) 97, 269, 748, 975
Alw21I GWGCWC 2 cut(s) 488, 707
Alw26I GTCTC 2 cut(s) 704, 737
Alw44I GTGCAC 1 cut(s) 484
AlwI GGATC 4 cut(s) 146, 159, 338, 393
AoxI GGCC 1 cut(s) 230
ApaLI GTGCAC 1 cut(s) 484
ApeKI GCWGC 4 cut(s) 83, 86, 725, 781
ApoI RAATTY 1 cut(s) 258
Asp700I GAANNNNTTC 1 cut(s) 114
AsuC2I CCSGG 2 cut(s) 156, 933
AsuHPI GGTGA 1 cut(s) 1000
AsuNHI GCTAGC 1 cut(s) 97
AxyI CCTNAGG 1 cut(s) 325
BaeGI GKGCMC 1 cut(s) 488
BaeI ACNNNNGTAYC 2 cut(s) 444, 477
BamHI GGATCC 1 cut(s) 151
Bbv12I GWGCWC 2 cut(s) 488, 707
BbvI GCAGC 4 cut(s) 70, 73, 737, 768
BccI CCATC 2 cut(s) 659, 987
BclI TGATCA 1 cut(s) 867
BcnI CCSGG 2 cut(s) 156, 933
BcoDI GTCTC 2 cut(s) 704, 737
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 2 cut(s) 90, 456
BfuAI ACCTGC 1 cut(s) 947
BglII AGATCT 1 cut(s) 536
BisI GCNGC 4 cut(s) 84, 87, 726, 782
BlsI GCNGC 4 cut(s) 85, 88, 727, 783
Bme1390I CCNGG 2 cut(s) 156, 933
BmiI GGNNCC 2 cut(s) 153, 937
BmrFI CCNGG 2 cut(s) 156, 933
BmsI GCATC 1 cut(s) 754
BmtI GCTAGC 1 cut(s) 101
Bpu10I CCTNAGC 1 cut(s) 334
BpuMI CCSGG 2 cut(s) 156, 933
BsaWI WCCGGW 1 cut(s) 236
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 206, 653, 774
Bse1I ACTGG 2 cut(s) 313, 396
Bse21I CCTNAGG 1 cut(s) 325
Bse3DI GCAATG 3 cut(s) 256, 821, 949
BseGI GGATG 3 cut(s) 834, 884, 979
BseLI CCNNNNNNNGG 3 cut(s) 206, 653, 774
BseMI GCAATG 3 cut(s) 256, 821, 949
BseMII CTCAG 3 cut(s) 339, 348, 671
BseNI ACTGG 2 cut(s) 313, 396
BseSI GKGCMC 1 cut(s) 488
BseXI GCAGC 4 cut(s) 70, 73, 737, 768
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 1 cut(s) 232
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 2 cut(s) 488, 707
BsiSI CCGG 4 cut(s) 119, 155, 237, 932
BslFI GGGAC 3 cut(s) 227, 901, 983
BslI CCNNNNNNNGG 3 cut(s) 206, 653, 774
BsmAI GTCTC 2 cut(s) 704, 737
BsmFI GGGAC 3 cut(s) 227, 901, 983
BsmI GAATGC 1 cut(s) 909
BsnI GGCC 1 cut(s) 232
Bsp1286I GDGCHC 2 cut(s) 488, 707
Bsp1407I TGTACA 1 cut(s) 917
Bsp143I GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
BspACI CCGC 1 cut(s) 753
BspANI GGCC 1 cut(s) 232
BspCNI CTCAG 3 cut(s) 338, 347, 672
BspLI GGNNCC 2 cut(s) 153, 937
BspMI ACCTGC 1 cut(s) 947
BspOI GCTAGC 1 cut(s) 101
BspPI GGATC 4 cut(s) 146, 159, 338, 393
BsrDI GCAATG 3 cut(s) 256, 821, 949
BsrGI TGTACA 1 cut(s) 917
BsrI ACTGG 2 cut(s) 313, 396
BssMI GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
BssNAI GTATAC 1 cut(s) 561
Bst1107I GTATAC 1 cut(s) 561
Bst4CI ACNGT 1 cut(s) 666
Bst6I CTCTTC 1 cut(s) 740
BstAUI TGTACA 1 cut(s) 917
BstC8I GCNNGC 3 cut(s) 99, 267, 648
BstDEI CTNAG 5 cut(s) 325, 334, 530, 680, 691
BstENI CCTNNNNNAGG 1 cut(s) 772
BstF5I GGATG 3 cut(s) 834, 884, 979
BstKTI GATC 7 cut(s) 66, 154, 346, 388, 539, 568, 870
BstMAI GTCTC 2 cut(s) 704, 737
BstMBI GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 1 cut(s) 722
BstSCI CCNGG 2 cut(s) 154, 931
BstSFI CTRYAG 2 cut(s) 90, 456
BstSLI GKGCMC 1 cut(s) 488
BstV1I GCAGC 4 cut(s) 70, 73, 737, 768
BstX2I RGATCY 2 cut(s) 151, 536
BstYI RGATCY 2 cut(s) 151, 536
BstZ17I GTATAC 1 cut(s) 561
Bsu36I CCTNAGG 1 cut(s) 325
BsuRI GGCC 1 cut(s) 232
BtsCI GGATG 3 cut(s) 834, 884, 979
BtsI GCAGTG 1 cut(s) 129
BtsIMutI CAGTG 2 cut(s) 129, 677
BveI ACCTGC 1 cut(s) 947
Cac8I GCNNGC 3 cut(s) 99, 267, 648
Csp6I GTAC 2 cut(s) 662, 918
CviAII CATG 1 cut(s) 571
CviQI GTAC 2 cut(s) 662, 918
DdeI CTNAG 5 cut(s) 325, 334, 530, 680, 691
DpnI GATC 7 cut(s) 65, 153, 345, 387, 538, 567, 869
DpnII GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
DraIII CACNNNGTG 1 cut(s) 683
Eam1104I CTCTTC 1 cut(s) 740
EarI CTCTTC 1 cut(s) 740
EciI GGCGGA 1 cut(s) 742
Eco147I AGGCCT 1 cut(s) 232
Eco32I GATATC 1 cut(s) 811
Eco57I CTGAAG 2 cut(s) 308, 969
Eco81I CCTNAGG 1 cut(s) 325
EcoNI CCTNNNNNAGG 1 cut(s) 772
EcoRV GATATC 1 cut(s) 811
FaeI CATG 1 cut(s) 574
FalI AAGNNNNNCTT 4 cut(s) 288, 320, 780, 812
FaqI GGGAC 3 cut(s) 227, 901, 983
FatI CATG 1 cut(s) 570
FbaI TGATCA 1 cut(s) 867
FblI GTMKAC 2 cut(s) 560, 801
Fnu4HI GCNGC 4 cut(s) 84, 87, 726, 782
FokI GGATG 3 cut(s) 821, 891, 966
Fsp4HI GCNGC 4 cut(s) 84, 87, 726, 782
FspBI CTAG 1 cut(s) 98
GluI GCNGC 4 cut(s) 84, 87, 726, 782
HaeIII GGCC 1 cut(s) 232
HapII CCGG 4 cut(s) 119, 155, 237, 932
Hin1II CATG 1 cut(s) 574
HinfI GANTC 7 cut(s) 115, 140, 278, 292, 612, 947, 1025
HpaII CCGG 4 cut(s) 119, 155, 237, 932
HphI GGTGA 1 cut(s) 1000
Hpy166II GTNNAC 6 cut(s) 486, 561, 662, 802, 918, 920
Hpy188I TCNGA 7 cut(s) 288, 385, 507, 617, 709, 988, 1049
Hpy188III TCNNGA 4 cut(s) 50, 296, 336, 599
Hpy8I GTNNAC 6 cut(s) 486, 561, 662, 802, 918, 920
Hpy99I CGWCG 1 cut(s) 955
HpyAV CCTTC 6 cut(s) 39, 204, 234, 433, 778, 993
HpyCH4III ACNGT 1 cut(s) 666
HpyCH4IV ACGT 1 cut(s) 922
HpyCH4V TGCA 4 cut(s) 192, 428, 486, 942
HpyF10VI GCNNNNNNNGC 1 cut(s) 722
HpyF3I CTNAG 5 cut(s) 325, 334, 530, 680, 691
HpySE526I ACGT 1 cut(s) 922
Hsp92II CATG 1 cut(s) 574
Ksp22I TGATCA 1 cut(s) 867
Kzo9I GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
LmnI GCTCC 1 cut(s) 617
Lsp1109I GCAGC 4 cut(s) 70, 73, 737, 768
LweI GCATC 1 cut(s) 754
MaeI CTAG 1 cut(s) 98
MaeII ACGT 1 cut(s) 922
MaeIII GTNAC 2 cut(s) 494, 666
MalI GATC 7 cut(s) 65, 153, 345, 387, 538, 567, 869
MboI GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
MboII GAAGA 7 cut(s) 17, 36, 301, 546, 636, 727, 1036
MflI RGATCY 2 cut(s) 151, 536
MhlI GDGCHC 2 cut(s) 488, 707
MluCI AATT 3 cut(s) 193, 258, 627
MlyI GAGTC 2 cut(s) 272, 606
MroXI GAANNNNTTC 1 cut(s) 114
MseI TTAA 1 cut(s) 576
MspI CCGG 4 cut(s) 119, 155, 237, 932
MspR9I CCNGG 2 cut(s) 156, 933
Mva1269I GAATGC 1 cut(s) 909
MwoI GCNNNNNNNGC 1 cut(s) 722
NciI CCSGG 2 cut(s) 156, 933
NdeII GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
NheI GCTAGC 1 cut(s) 97
NlaIII CATG 1 cut(s) 574
NlaIV GGNNCC 2 cut(s) 153, 937
NmuCI GTSAC 1 cut(s) 666
PceI AGGCCT 1 cut(s) 232
PctI GAATGC 1 cut(s) 909
PdmI GAANNNNTTC 1 cut(s) 114
PfeI GAWTC 5 cut(s) 115, 140, 292, 947, 1025
PflMI CCANNNNNTGG 1 cut(s) 206
PfoI TCCNGGA 2 cut(s) 154, 931
PkrI GCNGC 4 cut(s) 85, 88, 727, 783
Ple19I CGATCG 1 cut(s) 66
PleI GAGTC 2 cut(s) 272, 606
PpsI GAGTC 2 cut(s) 272, 606
PsiI TTATAA 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 153, 937
PsuI RGATCY 2 cut(s) 151, 536
PvuI CGATCG 1 cut(s) 66
RsaI GTAC 2 cut(s) 663, 919
RsaNI GTAC 2 cut(s) 662, 918
SaqAI TTAA 1 cut(s) 576
SatI GCNGC 4 cut(s) 84, 87, 726, 782
Sau3AI GATC 7 cut(s) 63, 151, 343, 385, 536, 565, 867
SchI GAGTC 2 cut(s) 272, 606
ScrFI CCNGG 2 cut(s) 156, 933
SduI GDGCHC 2 cut(s) 488, 707
SfaNI GCATC 1 cut(s) 754
SfcI CTRYAG 2 cut(s) 90, 456
Sse9I AATT 3 cut(s) 193, 258, 627
SseBI AGGCCT 1 cut(s) 232
SsiI CCGC 1 cut(s) 753
SspI AATATT 1 cut(s) 172
SspMI CTAG 1 cut(s) 98
StuI AGGCCT 1 cut(s) 232
StyD4I CCNGG 2 cut(s) 154, 931
TaaI ACNGT 1 cut(s) 666
TaiI ACGT 1 cut(s) 925
TaqI TCGA 6 cut(s) 51, 464, 807, 874, 953, 1023
TasI AATT 3 cut(s) 193, 258, 627
TatI WGTACW 1 cut(s) 917
TfiI GAWTC 5 cut(s) 115, 140, 292, 947, 1025
Tru1I TTAA 1 cut(s) 576
Tru9I TTAA 1 cut(s) 576
TscAI CASTG 2 cut(s) 129, 684
TseFI GTSAC 1 cut(s) 666
TseI GCWGC 4 cut(s) 83, 86, 725, 781
Tsp45I GTSAC 1 cut(s) 666
TspDTI ATGAA 4 cut(s) 17, 21, 1014, 1025
TspRI CASTG 2 cut(s) 129, 684
Van91I CCANNNNNTGG 1 cut(s) 206
VneI GTGCAC 1 cut(s) 484
XagI CCTNNNNNAGG 1 cut(s) 772
XapI RAATTY 1 cut(s) 258
XmiI GTMKAC 2 cut(s) 560, 801
XmnI GAANNNNTTC 1 cut(s) 114
XspI CTAG 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.