Rroxscaffold_7G00164780

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0000393
Physical Location & Seq
Reverse (-)
6677310 .. 6678005
696 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00164780.1

Sequence Viewer

Length: 483 bp
ATGACTTACAATGTCCTATGGTTGTTTCTTTCTCTGGATCTGATATTTACCAGTGCCGTAGCTATTGTTAAGCTACCGAAAAATGCAACGGTTCCTGCTGTTTTTATGTTCGGAGATTCGATAGTCGACACAGGCAACAATAACAACCTAACAACCATTATAAAAAGCAATTTCCCTCCCTACGGAAGAGACTTTCCCGGAGGAGTTCCAACCGGAAGATTTAGCAACGGCAAGGTTGCATCCGACTTCGTTGTGGAGGAACTGGGGGTCAAAGAATACTTGCCAGCCTATCTTGATCCAGATCTACAAGAGACGGACTTGCTTACTGGAGTAAACTTTGCTTCAAGCGGCTCAGGGTATGATCCCTTGACATCTACAATTATGAATGCTATGCCATTATCAAAACAGTTAGACATGTTGAAGGAATGCATTGAGAAGCTGAACAAGTATGTCGGAGAAGAGAGAAGGCGAACAGCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

160

Amino Acids

17.59

Weight (kDa)

4.8

Isoelectric Point (pI)

38.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 133 5.3e-09 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 161, 481
AccI GTMKAC 1 cut(s) 126
AciI CCGC 1 cut(s) 348
AclWI GGATC 3 cut(s) 45, 290, 356
AfiI CCNNNNNNNGG 1 cut(s) 182
AflIII ACRYGT 1 cut(s) 414
AgsI TTSAA 2 cut(s) 345, 421
AluBI AGCT 3 cut(s) 62, 73, 439
AluI AGCT 3 cut(s) 62, 73, 439
Alw26I GTCTC 2 cut(s) 183, 305
AlwI GGATC 3 cut(s) 45, 290, 356
AsuC2I CCSGG 1 cut(s) 198
BceAI ACGGC 2 cut(s) 41, 244
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 183, 305
BglII AGATCT 1 cut(s) 301
BisI GCNGC 1 cut(s) 349
BlsI GCNGC 1 cut(s) 350
Bme1390I CCNGG 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 93
BmrFI CCNGG 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 1 cut(s) 248
BmuI ACTGGG 1 cut(s) 272
BpmI CTGGAG 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 198
BsaBI GATNNNNATC 1 cut(s) 300
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 1 cut(s) 182
Bse1I ACTGG 3 cut(s) 51, 267, 331
Bse8I GATNNNNATC 1 cut(s) 300
BseGI GGATG 1 cut(s) 239
BseJI GATNNNNATC 1 cut(s) 300
BseLI CCNNNNNNNGG 1 cut(s) 182
BseMII CTCAG 1 cut(s) 366
BseNI ACTGG 3 cut(s) 51, 267, 331
BseRI GAGGAG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 198, 213
BslI CCNNNNNNNGG 1 cut(s) 182
BsmAI GTCTC 2 cut(s) 183, 305
BsmBI CGTCTC 1 cut(s) 305
BsmI GAATGC 2 cut(s) 391, 431
Bsp143I GATC 4 cut(s) 37, 295, 301, 361
BspACI CCGC 1 cut(s) 348
BspCNI CTCAG 1 cut(s) 365
BspLI GGNNCC 1 cut(s) 93
BspPI GGATC 3 cut(s) 45, 290, 356
BsrI ACTGG 3 cut(s) 51, 267, 331
BssMI GATC 4 cut(s) 37, 295, 301, 361
Bst4CI ACNGT 2 cut(s) 91, 408
Bst6I CTCTTC 2 cut(s) 181, 453
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 1 cut(s) 352
BstF5I GGATG 1 cut(s) 239
BstKTI GATC 4 cut(s) 40, 298, 304, 364
BstMAI GTCTC 2 cut(s) 183, 305
BstMBI GATC 4 cut(s) 37, 295, 301, 361
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 1 cut(s) 196
BstX2I RGATCY 2 cut(s) 37, 301
BstYI RGATCY 2 cut(s) 37, 301
BtsCI GGATG 1 cut(s) 239
BtsIMutI CAGTG 1 cut(s) 58
Cac8I GCNNGC 1 cut(s) 285
CviAII CATG 1 cut(s) 415
CviJI RGCY 5 cut(s) 62, 73, 287, 351, 439
CviKI_1 RGCY 5 cut(s) 62, 73, 287, 351, 439
DdeI CTNAG 1 cut(s) 352
DpnI GATC 4 cut(s) 39, 297, 303, 363
DpnII GATC 4 cut(s) 37, 295, 301, 361
Eam1104I CTCTTC 2 cut(s) 181, 453
EarI CTCTTC 2 cut(s) 181, 453
EcoT22I ATGCAT 1 cut(s) 431
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 1 cut(s) 418
FaiI YATR 9 cut(s) 19, 107, 161, 360, 383, 392, 416, 450, 481
FatI CATG 1 cut(s) 414
FblI GTMKAC 1 cut(s) 126
Fnu4HI GCNGC 1 cut(s) 349
FokI GGATG 1 cut(s) 226
Fsp4HI GCNGC 1 cut(s) 349
GluI GCNGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 348
HapII CCGG 2 cut(s) 198, 213
Hin1II CATG 1 cut(s) 418
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 1 cut(s) 116
HpaII CCGG 2 cut(s) 198, 213
Hpy166II GTNNAC 2 cut(s) 127, 334
Hpy188I TCNGA 4 cut(s) 42, 113, 244, 455
Hpy188III TCNNGA 3 cut(s) 35, 293, 299
Hpy8I GTNNAC 2 cut(s) 127, 334
HpyAV CCTTC 2 cut(s) 415, 459
HpyCH4III ACNGT 2 cut(s) 91, 408
HpyCH4V TGCA 3 cut(s) 86, 239, 429
HpyF3I CTNAG 1 cut(s) 352
Hsp92II CATG 1 cut(s) 418
Kzo9I GATC 4 cut(s) 37, 295, 301, 361
LweI GCATC 1 cut(s) 248
MalI GATC 4 cut(s) 39, 297, 303, 363
MboI GATC 4 cut(s) 37, 295, 301, 361
MboII GAAGA 3 cut(s) 198, 228, 470
MflI RGATCY 2 cut(s) 37, 301
MluCI AATT 2 cut(s) 169, 378
MmeI TCCRAC 3 cut(s) 233, 267, 433
MnlI CCTC 3 cut(s) 186, 194, 250
Mph1103I ATGCAT 1 cut(s) 431
MseI TTAA 1 cut(s) 69
MspI CCGG 2 cut(s) 198, 213
MspR9I CCNGG 1 cut(s) 198
Mva1269I GAATGC 2 cut(s) 391, 431
NciI CCSGG 1 cut(s) 198
NdeII GATC 4 cut(s) 37, 295, 301, 361
NlaIII CATG 1 cut(s) 418
NlaIV GGNNCC 1 cut(s) 93
NsiI ATGCAT 1 cut(s) 431
NspI RCATGY 1 cut(s) 418
PciI ACATGT 1 cut(s) 414
PctI GAATGC 2 cut(s) 391, 431
PfeI GAWTC 1 cut(s) 116
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 1 cut(s) 350
PscI ACATGT 1 cut(s) 414
PsiI TTATAA 2 cut(s) 161, 481
PspN4I GGNNCC 1 cut(s) 93
PsuI RGATCY 2 cut(s) 37, 301
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 1 cut(s) 349
Sau3AI GATC 4 cut(s) 37, 295, 301, 361
ScrFI CCNGG 1 cut(s) 198
SetI ASST 5 cut(s) 64, 75, 150, 237, 441
SfaNI GCATC 1 cut(s) 248
Sse9I AATT 2 cut(s) 169, 378
SsiI CCGC 1 cut(s) 348
StyD4I CCNGG 1 cut(s) 196
TaaI ACNGT 2 cut(s) 91, 408
TaqI TCGA 2 cut(s) 119, 126
TasI AATT 2 cut(s) 169, 378
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 1 cut(s) 116
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 1 cut(s) 58
TspDTI ATGAA 1 cut(s) 398
TspGWI ACGGA 2 cut(s) 198, 329
TspRI CASTG 1 cut(s) 58
XceI RCATGY 1 cut(s) 418
XmiI GTMKAC 1 cut(s) 126
Zsp2I ATGCAT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.