Rh2CG128500

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0000393
Physical Location & Seq
Reverse (-)
11466457 .. 11469145
2689 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG128500.1

Sequence Viewer

Length: 981 bp
ATGAATTTTCTCACCCAAAAACTTCCTCTCTTTTTTCCAGTCATCATTGTTACATTCATCATCTACCATAGTGCTGCTGCTTTAACACTACCAAAAAATGTAACGTTTCCGGCAGTGATTGTTTGGGGAGATTCAATAGTGGATCCCGGAAACAACAACGTAGTCAACACTATAGTAAAATGCAACTTTGAACCTTATGGGAGGGACTATGCCGGAGGAGTGCCTACCGGAAGGTTTAGCAACGGAAGAGTCCCCTCAGACTTGATAGCTGAAGAACTTGGAATCAAGAAGATTTTACCGGCTTATCTGGATCCGAACCTGCAGCTTGAAGACCTACTTACTGGTGTAAGTTTTGCCTCTGGTGGTTCTGGTTATGATCCCCTCACTCCCAAAATAGTGAATGTGTTATCATTATCAGATCAATTAGAGTTGTTCAAAGAGTACAAAAGCAAAATAGATGCAGCAGTTGGGGTGGAAAGAAGAGAAATTATAGTGTCAAAAGGAATACACATTGTGTGCATAGGAAGTGATGACATTGCAAACACTTACCTCTCTACACCATTCAGGAGCCCTCACTACGATATTCCAGCCTATACAGATCTCATGGCCAATTCAGCTTCAAAGTTCTTTCAGGGGCTTTATGGAGTAGGAGCAAGAAAGATCGGAGTAGTAGGTATACCAGCAATTGGGTGTGTGCCATCACAGAGGACAATGAGTGGAGACATAAAGAGAGGGTGTTCAGCCAAAGCCAATCGAGCAGCTAGCCTCTTCAACTCCAAGCTCTCTTCCCTAATAGAGTCCCTCAATAAGAAGCTTCCAGAAGCAAAGTTTGTCTACTTTGATATCTATAATCCACTGGTTTCCATCATCCAAAATCCTTCTAAATATGGTAATCAATCATCATCATCATCATATATTTTCATTATCTTTGACCAATATATTATTAGAGGCACCAACTATTATCCGGACTTTACTTGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

326

Amino Acids

35.67

Weight (kDa)

8.44

Isoelectric Point (pI)

33.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 295 2.1e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccB1I GGYRCC 1 cut(s) 950
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 2 cut(s) 676, 834
AccIII TCCGGA 1 cut(s) 964
AclI AACGTT 1 cut(s) 104
AclWI GGATC 5 cut(s) 137, 150, 305, 318, 371
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 1 cut(s) 4
AcuI CTGAAG 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 514
AfaI GTAC 1 cut(s) 443
AfiI CCNNNNNNNGG 1 cut(s) 686
AgsI TTSAA 6 cut(s) 135, 191, 329, 436, 621, 772
AluBI AGCT 6 cut(s) 269, 325, 617, 761, 781, 814
AluI AGCT 6 cut(s) 269, 325, 617, 761, 781, 814
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 5 cut(s) 137, 150, 305, 318, 371
Aor13HI TCCGGA 1 cut(s) 964
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 5 cut(s) 74, 77, 322, 461, 758
ApoI RAATTY 1 cut(s) 4
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 1 cut(s) 4
AsuNHI GCTAGC 1 cut(s) 761
BalI TGGCCA 1 cut(s) 608
BamHI GGATCC 2 cut(s) 142, 310
BanI GGYRCC 1 cut(s) 950
BanII GRGCYC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 5 cut(s) 61, 64, 334, 473, 770
BccI CCATC 2 cut(s) 706, 872
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 2 cut(s) 762, 979
BfmI CTRYAG 2 cut(s) 171, 320
BfuAI ACCTGC 1 cut(s) 327
BglII AGATCT 1 cut(s) 598
BisI GCNGC 5 cut(s) 75, 78, 323, 462, 759
BlsI GCNGC 5 cut(s) 76, 79, 324, 463, 760
Bme1390I CCNGG 1 cut(s) 147
BmiI GGNNCC 4 cut(s) 144, 312, 569, 952
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 448
BmtI GCTAGC 1 cut(s) 765
BpiI GAAGAC 1 cut(s) 336
BpuMI CCSGG 1 cut(s) 147
BsaWI WCCGGW 2 cut(s) 227, 964
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 3 cut(s) 38, 346, 861
Bse3DI GCAATG 1 cut(s) 534
BseAI TCCGGA 1 cut(s) 964
BseGI GGATG 1 cut(s) 867
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 1 cut(s) 270
BseNI ACTGG 3 cut(s) 38, 346, 861
BseRI GAGGAG 1 cut(s) 231
BseXI GCAGC 5 cut(s) 61, 64, 334, 473, 770
BshFI GGCC 1 cut(s) 608
BshNI GGYRCC 1 cut(s) 950
BsiSI CCGG 6 cut(s) 110, 147, 213, 228, 299, 965
BslFI GGGAC 3 cut(s) 218, 236, 784
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 3 cut(s) 218, 236, 784
BsnI GGCC 1 cut(s) 608
Bsp1286I GDGCHC 1 cut(s) 572
Bsp13I TCCGGA 1 cut(s) 964
Bsp143I GATC 6 cut(s) 142, 310, 376, 418, 598, 660
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 1 cut(s) 269
BspEI TCCGGA 1 cut(s) 964
BspLI GGNNCC 4 cut(s) 144, 312, 569, 952
BspMAI CTGCAG 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 327
BspOI GCTAGC 1 cut(s) 765
BspPI GGATC 5 cut(s) 137, 150, 305, 318, 371
BspT107I GGYRCC 1 cut(s) 950
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 3 cut(s) 38, 346, 861
BssAI RCCGGY 1 cut(s) 298
BssMI GATC 6 cut(s) 142, 310, 376, 418, 598, 660
BssNAI GTATAC 1 cut(s) 677
Bst1107I GTATAC 1 cut(s) 677
Bst6I CTCTTC 4 cut(s) 241, 475, 773, 790
BstC8I GCNNGC 1 cut(s) 763
BstDEI CTNAG 1 cut(s) 256
BstF5I GGATG 1 cut(s) 867
BstKTI GATC 6 cut(s) 145, 313, 379, 421, 601, 663
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 6 cut(s) 142, 310, 376, 418, 598, 660
BstMWI GCNNNNNNNGC 2 cut(s) 614, 755
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 2 cut(s) 171, 320
BstV1I GCAGC 5 cut(s) 61, 64, 334, 473, 770
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 3 cut(s) 142, 310, 598
BstYI RGATCY 3 cut(s) 142, 310, 598
BstZ17I GTATAC 1 cut(s) 677
BsuRI GGCC 1 cut(s) 608
BtsCI GGATG 1 cut(s) 867
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 120, 854
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 298
Csp6I GTAC 1 cut(s) 442
CviAII CATG 1 cut(s) 604
CviQI GTAC 1 cut(s) 442
DdeI CTNAG 1 cut(s) 256
DpnI GATC 6 cut(s) 144, 312, 378, 420, 600, 662
DpnII GATC 6 cut(s) 142, 310, 376, 418, 598, 660
DraIII CACNNNGTG 1 cut(s) 514
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 4 cut(s) 241, 475, 773, 790
EarI CTCTTC 4 cut(s) 241, 475, 773, 790
Eco24I GRGCYC 1 cut(s) 572
Eco32I GATATC 1 cut(s) 844
Eco57I CTGAAG 1 cut(s) 291
EcoRV GATATC 1 cut(s) 844
EcoT38I GRGCYC 1 cut(s) 572
FaeI CATG 1 cut(s) 607
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 3 cut(s) 218, 236, 784
FatI CATG 1 cut(s) 603
FblI GTMKAC 2 cut(s) 676, 834
Fnu4HI GCNGC 5 cut(s) 75, 78, 323, 462, 759
FokI GGATG 1 cut(s) 854
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 5 cut(s) 75, 78, 323, 462, 759
FspBI CTAG 2 cut(s) 762, 979
GluI GCNGC 5 cut(s) 75, 78, 323, 462, 759
HaeIII GGCC 1 cut(s) 608
HapII CCGG 6 cut(s) 110, 147, 213, 228, 299, 965
Hin1II CATG 1 cut(s) 607
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 812
HinfI GANTC 4 cut(s) 131, 249, 282, 797
HpaII CCGG 6 cut(s) 110, 147, 213, 228, 299, 965
HphI GGTGA 1 cut(s) 4
Hpy166II GTNNAC 3 cut(s) 166, 677, 835
Hpy188I TCNGA 4 cut(s) 259, 315, 418, 665
Hpy188III TCNNGA 5 cut(s) 286, 308, 565, 818, 965
Hpy8I GTNNAC 3 cut(s) 166, 677, 835
HpyAV CCTTC 2 cut(s) 225, 888
HpyCH4IV ACGT 2 cut(s) 104, 159
HpyCH4V TGCA 5 cut(s) 183, 322, 461, 519, 539
HpyF10VI GCNNNNNNNGC 2 cut(s) 614, 755
HpyF3I CTNAG 1 cut(s) 256
HpySE526I ACGT 2 cut(s) 104, 159
Hsp92II CATG 1 cut(s) 607
Kpn2I TCCGGA 1 cut(s) 964
Kzo9I GATC 6 cut(s) 142, 310, 376, 418, 598, 660
LmnI GCTCC 2 cut(s) 567, 650
Lsp1109I GCAGC 5 cut(s) 61, 64, 334, 473, 770
LweI GCATC 1 cut(s) 448
MaeI CTAG 2 cut(s) 762, 979
MaeII ACGT 2 cut(s) 104, 159
MaeIII GTNAC 2 cut(s) 49, 100
MalI GATC 6 cut(s) 144, 312, 378, 420, 600, 662
MboI GATC 6 cut(s) 142, 310, 376, 418, 598, 660
MboII GAAGA 7 cut(s) 258, 284, 301, 341, 492, 760, 777
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 3 cut(s) 142, 310, 598
MhlI GDGCHC 1 cut(s) 572
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 5 cut(s) 4, 422, 486, 610, 684
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 2 cut(s) 258, 806
Mox20I TGGCCA 1 cut(s) 608
MroI TCCGGA 1 cut(s) 964
MscI TGGCCA 1 cut(s) 608
MseI TTAA 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 608
MspI CCGG 6 cut(s) 110, 147, 213, 228, 299, 965
MspR9I CCNGG 1 cut(s) 147
MunI CAATTG 1 cut(s) 684
MwoI GCNNNNNNNGC 2 cut(s) 614, 755
NciI CCSGG 1 cut(s) 147
NdeII GATC 6 cut(s) 142, 310, 376, 418, 598, 660
NheI GCTAGC 1 cut(s) 761
NlaIII CATG 1 cut(s) 607
NlaIV GGNNCC 4 cut(s) 144, 312, 569, 952
PfeI GAWTC 2 cut(s) 131, 282
PflMI CCANNNNNTGG 1 cut(s) 686
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 5 cut(s) 76, 79, 324, 463, 760
PleI GAGTC 2 cut(s) 257, 805
PpsI GAGTC 2 cut(s) 257, 805
Psp1406I AACGTT 1 cut(s) 104
PspN4I GGNNCC 4 cut(s) 144, 312, 569, 952
PstI CTGCAG 1 cut(s) 324
PsuI RGATCY 3 cut(s) 142, 310, 598
RsaI GTAC 1 cut(s) 443
RsaNI GTAC 1 cut(s) 442
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 5 cut(s) 75, 78, 323, 462, 759
Sau3AI GATC 6 cut(s) 142, 310, 376, 418, 598, 660
SchI GAGTC 2 cut(s) 258, 806
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 1 cut(s) 572
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 2 cut(s) 171, 320
Sse9I AATT 5 cut(s) 4, 422, 486, 610, 684
SspMI CTAG 2 cut(s) 762, 979
StyD4I CCNGG 1 cut(s) 145
TaiI ACGT 2 cut(s) 107, 162
TaqI TCGA 1 cut(s) 754
TasI AATT 5 cut(s) 4, 422, 486, 610, 684
TatI WGTACW 1 cut(s) 441
TfiI GAWTC 2 cut(s) 131, 282
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 2 cut(s) 120, 861
TseI GCWGC 5 cut(s) 74, 77, 322, 461, 758
TspDTI ATGAA 3 cut(s) 17, 46, 910
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 120, 861
Van91I CCANNNNNTGG 1 cut(s) 686
XapI RAATTY 1 cut(s) 4
XmiI GTMKAC 2 cut(s) 676, 834
XspI CTAG 2 cut(s) 762, 979
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.