MD15G1008700.v1.1

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000393
Physical Location & Seq
Reverse (-)
455418 .. 456898
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1008700.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGACCAGTTTATTTTTCTTTTTGCTTGGTTCTCTAGACCTGATATTCTCTACTGCCCAAGCAACTGTGAAGCTACCAAAAAATGTTACAATTCCTGCTGTTTTTATGTTCGGGGATTCAACCGTTGATACAGGCAACAACAACAACCTTGTAACAATTATTAAAAGCAATTTTCCCCCATATGGAAGAGATTTTATGGGAGGAGTAGCTACCGGAAGATTTGGCAATGGCAAGGTGCCCTCAGACATCTTTGTGGAGGAACTGGGAATCAAAAAACTTTTGCCGGCGTATCTTGATCCAAGTCTCCAAAAGAAGGACTTTCCCACCGGAGTAAGCTTTGCTTCAGGCGCTTCAGGATTTGATCCATTAACATCTGAGATGATGTATGATAAAACAAATGATCATATTCAGTCTGTTATACCGCTATCAGAACAATTACTACTGCTGAAAGAATACAAAGAGAAGCTGAAAAATTATGTTGGAGGAAAGAGAGCAAAGAGCATTGTAAGCAAGAGCCTACACTTTGTGGTAACAGGCAGTGACGACCTAGTGAATACCTACTTTCATACACCTGCACGCTCCTTGCAATACGATATAGATGCCTACACTGATTTTATGGTGGTCGAGGCTTCAGCCTTCCTGCAGGAATTATATGCATCGAGAGCACGGAGGATTGTCATTTCTGGGCTACCGCCGGTTGGATGTTTGCCATCAATGAGAACTGTAGACGGAGGTTCAGAAAGAAATTGTGTTGCGAGATACAATCAAGCAGCAGAGTTGTTCAACTCCAAGCTATCCGCGGAGGTGGACCGCCTTAACAAGCAGCTATTCCATGCCAAAGTGGTGGTTTTGATGGATGTCTACCGTCCACTCATGGATATCATCCTCAAGCCACAAAAATATGGATTTAAAGTTGAAGACAAAGGTTGTTGTGGAACAGGAAGAATAGAGGTAGTCAGATTGTGCAATCGATTGTCACCAAACACATGCAACAATACCAGAGAGTACGTTTTCTGGGACAGTTATCATCCAACTGAAAGAGTTTACAAGTTATTAGTCCCAAAGTTACTCCAAAAGTGCATTAACGACTTATTCAGCCAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

369

Amino Acids

41.2

Weight (kDa)

8.89

Isoelectric Point (pI)

28.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 352 2.5e-26 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 580
Acc36I ACCTGC 1 cut(s) 580
AccB1I GGYRCC 1 cut(s) 235
AccI GTMKAC 2 cut(s) 726, 861
AccII CGCG 1 cut(s) 800
AciI CCGC 5 cut(s) 422, 692, 798, 800, 811
AclWI GGATC 2 cut(s) 290, 356
AcuI CTGAAG 3 cut(s) 327, 336, 615
AdeI CACNNNGTG 1 cut(s) 526
AfaI GTAC 1 cut(s) 1007
AfiI CCNNNNNNNGG 3 cut(s) 182, 313, 698
AgsI TTSAA 3 cut(s) 120, 784, 917
AluBI AGCT 6 cut(s) 73, 209, 336, 466, 793, 826
AluI AGCT 6 cut(s) 73, 209, 336, 466, 793, 826
Alw21I GWGCWC 1 cut(s) 667
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 2 cut(s) 290, 356
ApeKI GCWGC 2 cut(s) 770, 823
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 1 cut(s) 808
AsuHPI GGTGA 1 cut(s) 969
AvaII GGWCC 1 cut(s) 808
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 924
Bbv12I GWGCWC 1 cut(s) 667
BbvI GCAGC 2 cut(s) 782, 835
BccI CCATC 2 cut(s) 718, 847
BcgI CGANNNNNNTGC 2 cut(s) 581, 615
BclI TGATCA 1 cut(s) 400
BcoDI GTCTC 1 cut(s) 308
BfaI CTAG 2 cut(s) 35, 548
BfmI CTRYAG 2 cut(s) 641, 723
BfoI RGCGCY 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 580
BisI GCNGC 2 cut(s) 771, 824
BlsI GCNGC 2 cut(s) 772, 825
Bme18I GGWCC 1 cut(s) 808
BmgT120I GGNCC 1 cut(s) 808
BmiI GGNNCC 1 cut(s) 237
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 2 cut(s) 589, 665
BmuI ACTGGG 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 924
BpuEI CTTGAG 1 cut(s) 872
Bsa29I ATCGAT 1 cut(s) 970
BsaJI CCNNGG 1 cut(s) 798
BsaWI WCCGGW 2 cut(s) 212, 326
Bsc4I CCNNNNNNNGG 3 cut(s) 182, 313, 698
Bse118I RCCGGY 2 cut(s) 283, 694
Bse1I ACTGG 2 cut(s) 6, 267
Bse3DI GCAATG 1 cut(s) 232
BseCI ATCGAT 1 cut(s) 970
BseDI CCNNGG 1 cut(s) 798
BseGI GGATG 4 cut(s) 707, 862, 882, 1027
BseLI CCNNNNNNNGG 3 cut(s) 182, 313, 698
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 2 cut(s) 255, 366
BseNI ACTGG 2 cut(s) 6, 267
BseRI GAGGAG 1 cut(s) 216
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 2 cut(s) 782, 835
BsgI GTGCAG 1 cut(s) 558
Bsh1236I CGCG 1 cut(s) 800
BshNI GGYRCC 1 cut(s) 235
BshVI ATCGAT 1 cut(s) 970
BsiHKAI GWGCWC 1 cut(s) 667
BsiSI CCGG 4 cut(s) 213, 284, 327, 695
BslFI GGGAC 2 cut(s) 1031, 1043
BslI CCNNNNNNNGG 3 cut(s) 182, 313, 698
BsmAI GTCTC 1 cut(s) 308
BsmFI GGGAC 2 cut(s) 1031, 1043
Bsp1286I GDGCHC 2 cut(s) 240, 667
Bsp143I GATC 3 cut(s) 295, 361, 400
BspACI CCGC 5 cut(s) 422, 692, 798, 800, 811
BspCNI CTCAG 2 cut(s) 254, 367
BspDI ATCGAT 1 cut(s) 970
BspFNI CGCG 1 cut(s) 800
BspLI GGNNCC 1 cut(s) 237
BspMAI CTGCAG 1 cut(s) 645
BspMI ACCTGC 1 cut(s) 580
BspPI GGATC 2 cut(s) 290, 356
BspT107I GGYRCC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 232
BsrFI RCCGGY 2 cut(s) 283, 694
BsrI ACTGG 2 cut(s) 6, 267
BssAI RCCGGY 2 cut(s) 283, 694
BssECI CCNNGG 1 cut(s) 798
BssMI GATC 3 cut(s) 295, 361, 400
Bst4CI ACNGT 5 cut(s) 67, 124, 724, 866, 1022
Bst6I CTCTTC 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 285, 577
BstDEI CTNAG 2 cut(s) 241, 375
BstDSI CCRYGG 1 cut(s) 798
BstF5I GGATG 4 cut(s) 707, 862, 882, 1027
BstFNI CGCG 1 cut(s) 800
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 3 cut(s) 298, 364, 403
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 3 cut(s) 295, 361, 400
BstMWI GCNNNNNNNGC 3 cut(s) 347, 507, 662
BstNSI RCATGY 1 cut(s) 990
BstSFI CTRYAG 2 cut(s) 641, 723
BstSLI GKGCMC 1 cut(s) 240
BstUI CGCG 1 cut(s) 800
BstV1I GCAGC 2 cut(s) 782, 835
BstV2I GAAGAC 1 cut(s) 924
BstXI CCANNNNNNTGG 1 cut(s) 844
Bsu15I ATCGAT 1 cut(s) 970
BsuTUI ATCGAT 1 cut(s) 970
BtgI CCRYGG 1 cut(s) 798
BtsCI GGATG 4 cut(s) 707, 862, 882, 1027
BtsI GCAGTG 1 cut(s) 544
BtsIMutI CAGTG 2 cut(s) 544, 606
BveI ACCTGC 1 cut(s) 580
Cac8I GCNNGC 2 cut(s) 285, 577
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 2 cut(s) 283, 694
Cfr13I GGNCC 1 cut(s) 808
Cfr42I CCGCGG 1 cut(s) 801
ClaI ATCGAT 1 cut(s) 970
Csp6I GTAC 1 cut(s) 1006
CviAII CATG 3 cut(s) 833, 874, 987
CviQI GTAC 1 cut(s) 1006
DdeI CTNAG 2 cut(s) 241, 375
DpnI GATC 3 cut(s) 297, 363, 402
DpnII GATC 3 cut(s) 295, 361, 400
DraI TTTAAA 1 cut(s) 910
DraIII CACNNNGTG 1 cut(s) 526
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
Eco32I GATATC 1 cut(s) 880
Eco47I GGWCC 1 cut(s) 808
Eco57I CTGAAG 3 cut(s) 327, 336, 615
EcoRV GATATC 1 cut(s) 880
EcoT22I ATGCAT 1 cut(s) 658
FaeI CATG 3 cut(s) 836, 877, 990
FalI AAGNNNNNCTT 4 cut(s) 302, 334, 325, 357
FaqI GGGAC 2 cut(s) 1031, 1043
FatI CATG 3 cut(s) 832, 873, 986
FauNDI CATATG 1 cut(s) 181
FbaI TGATCA 1 cut(s) 400
FblI GTMKAC 2 cut(s) 726, 861
Fnu4HI GCNGC 2 cut(s) 771, 824
FokI GGATG 4 cut(s) 714, 869, 869, 1014
Fsp4HI GCNGC 2 cut(s) 771, 824
FspBI CTAG 2 cut(s) 35, 548
GlaI GCGC 1 cut(s) 349
GluI GCNGC 2 cut(s) 771, 824
HaeII RGCGCY 1 cut(s) 351
HapII CCGG 4 cut(s) 213, 284, 327, 695
HhaI GCGC 1 cut(s) 350
Hin1II CATG 3 cut(s) 836, 877, 990
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HindIII AAGCTT 1 cut(s) 334
HinfI GANTC 2 cut(s) 116, 267
HpaII CCGG 4 cut(s) 213, 284, 327, 695
HphI GGTGA 1 cut(s) 969
Hpy166II GTNNAC 5 cut(s) 727, 808, 862, 869, 1045
Hpy188I TCNGA 5 cut(s) 244, 376, 430, 739, 959
Hpy188III TCNNGA 4 cut(s) 35, 293, 354, 660
Hpy8I GTNNAC 5 cut(s) 727, 808, 862, 869, 1045
HpyAV CCTTC 2 cut(s) 307, 646
HpyCH4III ACNGT 5 cut(s) 67, 124, 724, 866, 1022
HpyCH4IV ACGT 1 cut(s) 1008
HpyCH4V TGCA 7 cut(s) 575, 586, 643, 656, 966, 990, 1080
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 507, 662
HpyF3I CTNAG 2 cut(s) 241, 375
HpySE526I ACGT 1 cut(s) 1008
Hsp92II CATG 3 cut(s) 836, 877, 990
HspAI GCGC 1 cut(s) 348
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Ksp22I TGATCA 1 cut(s) 400
KspI CCGCGG 1 cut(s) 801
Kzo9I GATC 3 cut(s) 295, 361, 400
LmnI GCTCC 1 cut(s) 584
Lsp1109I GCAGC 2 cut(s) 782, 835
LweI GCATC 2 cut(s) 589, 665
MaeI CTAG 2 cut(s) 35, 548
MaeII ACGT 1 cut(s) 1008
MaeIII GTNAC 6 cut(s) 85, 151, 529, 539, 975, 1065
MalI GATC 3 cut(s) 297, 363, 402
MboI GATC 3 cut(s) 295, 361, 400
MboII GAAGA 4 cut(s) 198, 228, 929, 954
MhlI GDGCHC 2 cut(s) 240, 667
MluCI AATT 7 cut(s) 90, 156, 169, 434, 472, 647, 745
MmeI TCCRAC 3 cut(s) 460, 679, 1055
Mph1103I ATGCAT 1 cut(s) 658
MroNI GCCGGC 1 cut(s) 283
MseI TTAA 5 cut(s) 162, 368, 816, 909, 1083
MslI CAYNNNNRTG 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 800
MspI CCGG 4 cut(s) 213, 284, 327, 695
MvnI CGCG 1 cut(s) 800
MwoI GCNNNNNNNGC 3 cut(s) 347, 507, 662
NaeI GCCGGC 1 cut(s) 285
NdeI CATATG 1 cut(s) 181
NdeII GATC 3 cut(s) 295, 361, 400
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 3 cut(s) 836, 877, 990
NlaIV GGNNCC 1 cut(s) 237
NmuCI GTSAC 2 cut(s) 539, 975
NsiI ATGCAT 1 cut(s) 658
NspI RCATGY 1 cut(s) 990
PaqCI CACCTGC 1 cut(s) 580
PdiI GCCGGC 1 cut(s) 285
PfeI GAWTC 2 cut(s) 116, 267
PkrI GCNGC 2 cut(s) 772, 825
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 1 cut(s) 808
PsrI GAACNNNNNNTAC 2 cut(s) 423, 455
PstI CTGCAG 1 cut(s) 645
RsaI GTAC 1 cut(s) 1007
RsaNI GTAC 1 cut(s) 1006
RseI CAYNNNNRTG 1 cut(s) 251
SacII CCGCGG 1 cut(s) 801
SaqAI TTAA 5 cut(s) 162, 368, 816, 909, 1083
SatI GCNGC 2 cut(s) 771, 824
Sau3AI GATC 3 cut(s) 295, 361, 400
Sau96I GGNCC 1 cut(s) 808
SbfI CCTGCAGG 1 cut(s) 645
SdaI CCTGCAGG 1 cut(s) 645
SduI GDGCHC 2 cut(s) 240, 667
SfaNI GCATC 2 cut(s) 589, 665
SfcI CTRYAG 2 cut(s) 641, 723
Sfr303I CCGCGG 1 cut(s) 801
SgrBI CCGCGG 1 cut(s) 801
SinI GGWCC 1 cut(s) 808
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 887
SmoI CTYRAG 1 cut(s) 887
Sse8387I CCTGCAGG 1 cut(s) 645
Sse9I AATT 7 cut(s) 90, 156, 169, 434, 472, 647, 745
SsiI CCGC 5 cut(s) 422, 692, 798, 800, 811
SspMI CTAG 2 cut(s) 35, 548
TaaI ACNGT 5 cut(s) 67, 124, 724, 866, 1022
TaiI ACGT 1 cut(s) 1011
TaqI TCGA 3 cut(s) 624, 659, 970
TasI AATT 7 cut(s) 90, 156, 169, 434, 472, 647, 745
TfiI GAWTC 2 cut(s) 116, 267
Tru1I TTAA 5 cut(s) 162, 368, 816, 909, 1083
Tru9I TTAA 5 cut(s) 162, 368, 816, 909, 1083
TscAI CASTG 2 cut(s) 544, 613
TseFI GTSAC 2 cut(s) 539, 975
TseI GCWGC 2 cut(s) 770, 823
Tsp45I GTSAC 2 cut(s) 539, 975
TspDTI ATGAA 1 cut(s) 554
TspGWI ACGGA 2 cut(s) 682, 744
TspRI CASTG 2 cut(s) 544, 613
VpaK11BI GGWCC 1 cut(s) 808
XbaI TCTAGA 1 cut(s) 34
XceI RCATGY 1 cut(s) 990
XmiI GTMKAC 2 cut(s) 726, 861
XspI CTAG 2 cut(s) 35, 548
Zsp2I ATGCAT 1 cut(s) 658
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.