pycom02g09920

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000393
Physical Location & Seq
Forward (+)
6861000 .. 6863169
2170 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g09920.4

Sequence Viewer

Length: 870 bp
ATGAACTTCCTCTCCCCAAAACTTCTTCCTTCATCATCTAAATCTTCTCTCGAACTTATAACGATCACCATATTCTTATTCTTGTACCGCAATGCTGCTGCTGTAGACCTACCGAACAACGAAAAGATTCCGGCAGTGATTGTTTTTGGAGATTCAATAGTGGATCCGGGAAACAACAACAATATTGGCACGATAGTCAAATGCAATTTCCCACCTTATGGGAGGGACTTTGTAGGAAAAAGGCCTACTGGAAGGTTTAGCAATGGCAGAGTCCCCTCAGACTTGATAGCTGAATCAGTTGGAGTGAAGAAGATTTTGCCAGCTTATTTGGACTCAAATCTGAAGATTCAAGACCTACTTACTGGTGTAAGTTTTGCCTCAGGTGGCTCAGGATATGATCCCCTCACTCCCAGAATAGTGGAACTATATGGACTCGGAGCAAGAAGAATTGGAGTTGTAAGTCTGCCAGCCATCGGGTGTGTACCGTCACAGAGAACGCTGAGTGGAGGCATAGACAGAGGGTGCTCGGAGACTGCCAACCAAGCAGCAAGTCTCTTCAACTCCAAGCTCTCTGCCCAAATAGATGCCTTCAATAGGAGGCTGCCAGAAGCAAGGCTTGTCTACCTCGATATCTATAACACATTGCTTTCCCTCGTCCAAAACCCTACTCAATATGGATTTGAAGTGATCAATCGAGGATGCTGTGGGACGGGAAAATTTGAGGTTAGCATTCTGTGTACACGTTACTCTCCGGGAACCTGCAATGATTCTTCGAAATACATATTCTGGGACAGCTACCATCCTTCAGAGAAAGGATATGAAGTCATCACCTCTCTGGTTTTCGATTCGCAAGTTCATAAGTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

290

Amino Acids

31.51

Weight (kDa)

8.61

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 59
Acc36I ACCTGC 1 cut(s) 767
AccB7I CCANNNNNTGG 1 cut(s) 218
AccI GTMKAC 2 cut(s) 105, 621
AciI CCGC 1 cut(s) 88
AclWI GGATC 3 cut(s) 158, 171, 392
AcsI RAATTY 1 cut(s) 716
AcuI CTGAAG 2 cut(s) 362, 789
AfaI GTAC 3 cut(s) 86, 483, 739
AfiI CCNNNNNNNGG 3 cut(s) 218, 473, 594
AflIII ACRYGT 1 cut(s) 740
AgsI TTSAA 5 cut(s) 156, 350, 559, 592, 683
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 290, 323, 568, 795
AluI AGCT 4 cut(s) 290, 323, 568, 795
Alw21I GWGCWC 1 cut(s) 527
Alw26I GTCTC 2 cut(s) 524, 557
AlwI GGATC 3 cut(s) 158, 171, 392
AoxI GGCC 1 cut(s) 242
ApeKI GCWGC 4 cut(s) 95, 98, 545, 601
ApoI RAATTY 1 cut(s) 716
Asp700I GAANNNNTTC 1 cut(s) 126
AsuC2I CCSGG 2 cut(s) 168, 753
AsuHPI GGTGA 2 cut(s) 58, 820
AsuII TTCGAA 1 cut(s) 773
AxyI CCTNAGG 1 cut(s) 379
BamHI GGATCC 1 cut(s) 163
Bbv12I GWGCWC 1 cut(s) 527
BbvI GCAGC 4 cut(s) 82, 85, 557, 588
BccI CCATC 2 cut(s) 479, 807
BclI TGATCA 1 cut(s) 687
BcnI CCSGG 2 cut(s) 168, 753
BcoDI GTCTC 2 cut(s) 524, 557
BfmI CTRYAG 1 cut(s) 102
BfuAI ACCTGC 1 cut(s) 767
BisI GCNGC 4 cut(s) 96, 99, 546, 602
BlsI GCNGC 4 cut(s) 97, 100, 547, 603
Bme1390I CCNGG 2 cut(s) 168, 753
BmiI GGNNCC 2 cut(s) 165, 757
BmrFI CCNGG 2 cut(s) 168, 753
BmsI GCATC 2 cut(s) 574, 689
Bpu10I CCTNAGC 1 cut(s) 388
Bpu14I TTCGAA 1 cut(s) 773
BpuMI CCSGG 2 cut(s) 168, 753
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 3 cut(s) 218, 473, 594
Bse1I ACTGG 2 cut(s) 253, 367
Bse21I CCTNAGG 1 cut(s) 379
Bse3DI GCAATG 4 cut(s) 97, 268, 641, 769
BseGI GGATG 2 cut(s) 704, 799
BseLI CCNNNNNNNGG 3 cut(s) 218, 473, 594
BseMI GCAATG 4 cut(s) 97, 268, 641, 769
BseMII CTCAG 4 cut(s) 291, 393, 402, 491
BseNI ACTGG 2 cut(s) 253, 367
BseXI GCAGC 4 cut(s) 82, 85, 557, 588
BshFI GGCC 1 cut(s) 244
BsiHKAI GWGCWC 1 cut(s) 527
BsiSI CCGG 3 cut(s) 131, 167, 752
BslFI GGGAC 4 cut(s) 239, 257, 721, 803
BslI CCNNNNNNNGG 3 cut(s) 218, 473, 594
BsmAI GTCTC 2 cut(s) 524, 557
BsmFI GGGAC 4 cut(s) 239, 257, 721, 803
BsmI GAATGC 1 cut(s) 729
BsnI GGCC 1 cut(s) 244
Bsp119I TTCGAA 1 cut(s) 773
Bsp1286I GDGCHC 1 cut(s) 527
Bsp1407I TGTACA 1 cut(s) 737
Bsp143I GATC 4 cut(s) 63, 163, 397, 687
BspACI CCGC 1 cut(s) 88
BspANI GGCC 1 cut(s) 244
BspCNI CTCAG 4 cut(s) 290, 392, 401, 492
BspLI GGNNCC 2 cut(s) 165, 757
BspMI ACCTGC 1 cut(s) 767
BspPI GGATC 3 cut(s) 158, 171, 392
BspT104I TTCGAA 1 cut(s) 773
BsrDI GCAATG 4 cut(s) 97, 268, 641, 769
BsrGI TGTACA 1 cut(s) 737
BsrI ACTGG 2 cut(s) 253, 367
BssMI GATC 4 cut(s) 63, 163, 397, 687
Bst4CI ACNGT 1 cut(s) 486
Bst6I CTCTTC 1 cut(s) 560
BstAUI TGTACA 1 cut(s) 737
BstBI TTCGAA 1 cut(s) 773
BstC8I GCNNGC 2 cut(s) 321, 468
BstDEI CTNAG 4 cut(s) 277, 379, 388, 500
BstENI CCTNNNNNAGG 1 cut(s) 592
BstF5I GGATG 2 cut(s) 704, 799
BstKTI GATC 4 cut(s) 66, 166, 400, 690
BstMAI GTCTC 2 cut(s) 524, 557
BstMBI GATC 4 cut(s) 63, 163, 397, 687
BstMWI GCNNNNNNNGC 1 cut(s) 542
BstSCI CCNGG 2 cut(s) 166, 751
BstSFI CTRYAG 1 cut(s) 102
BstV1I GCAGC 4 cut(s) 82, 85, 557, 588
BstX2I RGATCY 1 cut(s) 163
BstXI CCANNNNNNTGG 1 cut(s) 418
BstYI RGATCY 1 cut(s) 163
Bsu36I CCTNAGG 1 cut(s) 379
BsuRI GGCC 1 cut(s) 244
BtsCI GGATG 2 cut(s) 704, 799
BtsI GCAGTG 1 cut(s) 141
BtsIMutI CAGTG 1 cut(s) 141
BveI ACCTGC 1 cut(s) 767
Cac8I GCNNGC 2 cut(s) 321, 468
Csp6I GTAC 3 cut(s) 85, 482, 738
CviJI RGCY 9 cut(s) 244, 290, 323, 387, 470, 568, 601, 616, 795
CviKI_1 RGCY 9 cut(s) 244, 290, 323, 387, 470, 568, 601, 616, 795
CviQI GTAC 3 cut(s) 85, 482, 738
DdeI CTNAG 4 cut(s) 277, 379, 388, 500
DpnI GATC 4 cut(s) 65, 165, 399, 689
DpnII GATC 4 cut(s) 63, 163, 397, 687
Eam1104I CTCTTC 1 cut(s) 560
EarI CTCTTC 1 cut(s) 560
Eco147I AGGCCT 1 cut(s) 244
Eco32I GATATC 1 cut(s) 631
Eco57I CTGAAG 2 cut(s) 362, 789
Eco81I CCTNAGG 1 cut(s) 379
EcoNI CCTNNNNNAGG 1 cut(s) 592
EcoRV GATATC 1 cut(s) 631
FalI AAGNNNNNCTT 4 cut(s) 342, 374, 600, 632
FaqI GGGAC 4 cut(s) 239, 257, 721, 803
FbaI TGATCA 1 cut(s) 687
FblI GTMKAC 2 cut(s) 105, 621
Fnu4HI GCNGC 4 cut(s) 96, 99, 546, 602
FokI GGATG 2 cut(s) 711, 786
Fsp4HI GCNGC 4 cut(s) 96, 99, 546, 602
GluI GCNGC 4 cut(s) 96, 99, 546, 602
HaeIII GGCC 1 cut(s) 244
HapII CCGG 3 cut(s) 131, 167, 752
HinfI GANTC 9 cut(s) 127, 152, 270, 293, 332, 346, 432, 767, 845
HpaII CCGG 3 cut(s) 131, 167, 752
HphI GGTGA 2 cut(s) 58, 820
Hpy166II GTNNAC 5 cut(s) 106, 482, 622, 738, 740
Hpy188I TCNGA 6 cut(s) 280, 342, 437, 529, 808, 869
Hpy188III TCNNGA 3 cut(s) 50, 350, 390
Hpy8I GTNNAC 5 cut(s) 106, 482, 622, 738, 740
HpyAV CCTTC 4 cut(s) 39, 246, 598, 813
HpyCH4III ACNGT 1 cut(s) 486
HpyCH4IV ACGT 1 cut(s) 742
HpyCH4V TGCA 2 cut(s) 204, 762
HpyF10VI GCNNNNNNNGC 1 cut(s) 542
HpyF3I CTNAG 4 cut(s) 277, 379, 388, 500
HpySE526I ACGT 1 cut(s) 742
Ksp22I TGATCA 1 cut(s) 687
Kzo9I GATC 4 cut(s) 63, 163, 397, 687
LmnI GCTCC 1 cut(s) 437
Lsp1109I GCAGC 4 cut(s) 82, 85, 557, 588
LweI GCATC 2 cut(s) 574, 689
MaeII ACGT 1 cut(s) 742
MaeIII GTNAC 2 cut(s) 486, 743
MalI GATC 4 cut(s) 65, 165, 399, 689
MboI GATC 4 cut(s) 63, 163, 397, 687
MboII GAAGA 9 cut(s) 17, 36, 319, 322, 355, 456, 547, 762, 856
MflI RGATCY 1 cut(s) 163
MhlI GDGCHC 1 cut(s) 527
MluCI AATT 3 cut(s) 205, 447, 716
MlyI GAGTC 3 cut(s) 279, 326, 426
MmeI TCCRAC 1 cut(s) 280
MroXI GAANNNNTTC 1 cut(s) 126
MspI CCGG 3 cut(s) 131, 167, 752
MspR9I CCNGG 2 cut(s) 168, 753
Mva1269I GAATGC 1 cut(s) 729
MwoI GCNNNNNNNGC 1 cut(s) 542
NciI CCSGG 2 cut(s) 168, 753
NdeII GATC 4 cut(s) 63, 163, 397, 687
NlaIV GGNNCC 2 cut(s) 165, 757
NmuCI GTSAC 1 cut(s) 486
NspV TTCGAA 1 cut(s) 773
PceI AGGCCT 1 cut(s) 244
PctI GAATGC 1 cut(s) 729
PdmI GAANNNNTTC 1 cut(s) 126
PfeI GAWTC 6 cut(s) 127, 152, 293, 346, 767, 845
PflMI CCANNNNNTGG 1 cut(s) 218
PfoI TCCNGGA 2 cut(s) 166, 751
PkrI GCNGC 4 cut(s) 97, 100, 547, 603
PleI GAGTC 3 cut(s) 278, 326, 426
PpsI GAGTC 3 cut(s) 278, 326, 426
PsiI TTATAA 1 cut(s) 59
PspN4I GGNNCC 2 cut(s) 165, 757
PsuI RGATCY 1 cut(s) 163
RsaI GTAC 3 cut(s) 86, 483, 739
RsaNI GTAC 3 cut(s) 85, 482, 738
SatI GCNGC 4 cut(s) 96, 99, 546, 602
Sau3AI GATC 4 cut(s) 63, 163, 397, 687
SchI GAGTC 3 cut(s) 279, 326, 426
ScrFI CCNGG 2 cut(s) 168, 753
SduI GDGCHC 1 cut(s) 527
SfaNI GCATC 2 cut(s) 574, 689
SfcI CTRYAG 1 cut(s) 102
SfuI TTCGAA 1 cut(s) 773
Sse9I AATT 3 cut(s) 205, 447, 716
SseBI AGGCCT 1 cut(s) 244
SsiI CCGC 1 cut(s) 88
SspI AATATT 1 cut(s) 184
StuI AGGCCT 1 cut(s) 244
StyD4I CCNGG 2 cut(s) 166, 751
TaaI ACNGT 1 cut(s) 486
TaiI ACGT 1 cut(s) 745
TaqI TCGA 5 cut(s) 51, 627, 694, 773, 843
TasI AATT 3 cut(s) 205, 447, 716
TatI WGTACW 1 cut(s) 737
TfiI GAWTC 6 cut(s) 127, 152, 293, 346, 767, 845
TscAI CASTG 1 cut(s) 141
TseFI GTSAC 1 cut(s) 486
TseI GCWGC 4 cut(s) 95, 98, 545, 601
Tsp45I GTSAC 1 cut(s) 486
TspDTI ATGAA 4 cut(s) 17, 21, 834, 845
TspRI CASTG 1 cut(s) 141
Van91I CCANNNNNTGG 1 cut(s) 218
XagI CCTNNNNNAGG 1 cut(s) 592
XapI RAATTY 1 cut(s) 716
XmiI GTMKAC 2 cut(s) 105, 621
XmnI GAANNNNTTC 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.