Rw2G009550

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000393
Physical Location & Seq
Reverse (-)
9738539 .. 9740966
2428 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009550.1

Sequence Viewer

Length: 1083 bp
ATGAATTTTCTCACCCGAAAACTTCCTCTCTTTTTTCCAGTCATCATTGTTACATTCATCATCTACCATAGTGCTGCTGCTTTAACACTACCAAAAAATGTAACGTTTCCGGCAGTGATTGTTTGGGGAGATTCAATAGTGGATCCCGGAAACAACAACCAAGTCAACACTATAGTAAAATGCAACTTTGAACCTTATGGGAGGGACTACGCTGGAGGAGTGCCTACCGGAAGGTTTAGCAACGGAAGAGTCCCCTCAGACTTGATAGCTGAAGAACTTGGAATCAAGAAGATTTTACCGGCTTATCTGGATCCGAACCTGCAGCTTGAAGACCTACTTACTGGTGTAAGTTTTGCCTCTGGTGGTTCTGGTTATGATCCCCTCACTCCCAAAATAGTGAATGTGTTATCATTATCAGATCAATTAGAGTTGTTCAAAGAGTACAAAAGCAAAATAGATGCAGCAGTTGGGGTGGAAAGAAGAGAAATTATAGTGTCAAAAGGAGTACACGTTGTGTGCATAGGAAGTGATGACATTGCAAACACTTACCTCTCTACACCATTCAGGAGCCCTCACTACGATATTCCAGCCTATACAGATCTCATGGCCAATTCAGCTTCAAAGTTCTTTCAGGGGCTTTATGGAGTAGGAGCAAGAAAGATCGGAGTAGTAGGTATACCAGCAATTGGGTGTGTGCCATCACAGAGGACAATGAGTGGAGACATAAAGAGAGGGTGTTCAGCCAAAGCCAATCGAGCAGCTAGCCTCTTCAACTCCAAGCTCTCTTCCCTATTAGAGTCCCTCAATAAGAAGCTTCCAGAAGCAAAGTTTGTCTACTTTGATATCTATAATCCATTGGTTTCCATCATCCAAAATCCTTCTAAATATGGATTTGAAGTGGTGAATAAAGGGTGCTGCGGAACTGGAAACATAGAGGTCAGCATTCTTTGTACTCGTTTCTCTCTGGGCACTTGCAGCGATCCATCGAAATACATATTCTGGGACAGTTACCATCCCACTGAGAAGGCATACAAGACCCTTGTCCCCATGGCTTTCGATAAGGAAATTCACAAATTGTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

39.42

Weight (kDa)

8.62

Isoelectric Point (pI)

34.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 41 - 347 5.3e-31 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 327
AccB7I CCANNNNNTGG 1 cut(s) 686
AccI GTMKAC 2 cut(s) 676, 834
AciI CCGC 1 cut(s) 918
AclI AACGTT 1 cut(s) 104
AclWI GGATC 6 cut(s) 137, 150, 305, 318, 371, 974
AcoI YGGCCR 1 cut(s) 606
AcsI RAATTY 2 cut(s) 4, 1065
AcuI CTGAAG 1 cut(s) 291
AdeI CACNNNGTG 1 cut(s) 514
AfaI GTAC 3 cut(s) 443, 507, 952
AfiI CCNNNNNNNGG 1 cut(s) 686
AflIII ACRYGT 1 cut(s) 508
AgsI TTSAA 7 cut(s) 135, 191, 329, 436, 621, 772, 896
AluBI AGCT 6 cut(s) 269, 325, 617, 761, 781, 814
AluI AGCT 6 cut(s) 269, 325, 617, 761, 781, 814
Alw26I GTCTC 1 cut(s) 714
AlwI GGATC 6 cut(s) 137, 150, 305, 318, 371, 974
AoxI GGCC 1 cut(s) 606
ApeKI GCWGC 7 cut(s) 74, 77, 322, 461, 758, 915, 975
ApoI RAATTY 2 cut(s) 4, 1065
AsuC2I CCSGG 1 cut(s) 147
AsuHPI GGTGA 2 cut(s) 4, 913
AsuNHI GCTAGC 1 cut(s) 761
BaeGI GKGCMC 1 cut(s) 971
BalI TGGCCA 1 cut(s) 608
BamHI GGATCC 2 cut(s) 142, 310
BanII GRGCYC 1 cut(s) 572
BbsI GAAGAC 1 cut(s) 336
BbvI GCAGC 7 cut(s) 61, 64, 334, 473, 770, 902, 987
BccI CCATC 4 cut(s) 706, 872, 991, 1020
BcnI CCSGG 1 cut(s) 147
BcoDI GTCTC 1 cut(s) 714
BfaI CTAG 1 cut(s) 762
BfmI CTRYAG 2 cut(s) 171, 320
BfuAI ACCTGC 1 cut(s) 327
BglII AGATCT 1 cut(s) 598
BisI GCNGC 7 cut(s) 75, 78, 323, 462, 759, 916, 976
BlsI GCNGC 7 cut(s) 76, 79, 324, 463, 760, 917, 977
Bme1390I CCNGG 1 cut(s) 147
BmiI GGNNCC 3 cut(s) 144, 312, 569
BmrFI CCNGG 1 cut(s) 147
BmsI GCATC 1 cut(s) 448
BmtI GCTAGC 1 cut(s) 765
BoxI GACNNNNGTC 1 cut(s) 1040
BpiI GAAGAC 1 cut(s) 336
BpmI CTGGAG 1 cut(s) 234
BpuMI CCSGG 1 cut(s) 147
BsaJI CCNNGG 1 cut(s) 1047
BsaWI WCCGGW 1 cut(s) 227
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 1 cut(s) 686
Bse118I RCCGGY 1 cut(s) 298
Bse1I ACTGG 3 cut(s) 38, 346, 928
Bse3DI GCAATG 1 cut(s) 534
BseDI CCNNGG 1 cut(s) 1047
BseGI GGATG 2 cut(s) 867, 1012
BseLI CCNNNNNNNGG 1 cut(s) 686
BseMI GCAATG 1 cut(s) 534
BseMII CTCAG 2 cut(s) 270, 1011
BseNI ACTGG 3 cut(s) 38, 346, 928
BseRI GAGGAG 1 cut(s) 231
BseSI GKGCMC 1 cut(s) 971
BseXI GCAGC 7 cut(s) 61, 64, 334, 473, 770, 902, 987
BshFI GGCC 1 cut(s) 608
BsiSI CCGG 4 cut(s) 110, 147, 228, 299
BslFI GGGAC 5 cut(s) 218, 236, 784, 1016, 1028
BslI CCNNNNNNNGG 1 cut(s) 686
BsmAI GTCTC 1 cut(s) 714
BsmFI GGGAC 5 cut(s) 218, 236, 784, 1016, 1028
BsmI GAATGC 1 cut(s) 942
BsnI GGCC 1 cut(s) 608
Bsp1286I GDGCHC 2 cut(s) 572, 971
Bsp143I GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
Bsp19I CCATGG 1 cut(s) 1047
BspACI CCGC 1 cut(s) 918
BspANI GGCC 1 cut(s) 608
BspCNI CTCAG 2 cut(s) 269, 1012
BspLI GGNNCC 3 cut(s) 144, 312, 569
BspMAI CTGCAG 1 cut(s) 324
BspMI ACCTGC 1 cut(s) 327
BspOI GCTAGC 1 cut(s) 765
BspPI GGATC 6 cut(s) 137, 150, 305, 318, 371, 974
BsrDI GCAATG 1 cut(s) 534
BsrFI RCCGGY 1 cut(s) 298
BsrI ACTGG 3 cut(s) 38, 346, 928
BssAI RCCGGY 1 cut(s) 298
BssECI CCNNGG 1 cut(s) 1047
BssMI GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
BssNAI GTATAC 1 cut(s) 677
BssT1I CCWWGG 1 cut(s) 1047
Bst1107I GTATAC 1 cut(s) 677
Bst4CI ACNGT 1 cut(s) 1007
Bst6I CTCTTC 4 cut(s) 241, 475, 773, 790
BstC8I GCNNGC 1 cut(s) 763
BstDEI CTNAG 2 cut(s) 256, 1020
BstDSI CCRYGG 1 cut(s) 1047
BstF5I GGATG 2 cut(s) 867, 1012
BstKTI GATC 7 cut(s) 145, 313, 379, 421, 601, 663, 982
BstMAI GTCTC 1 cut(s) 714
BstMBI GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
BstMWI GCNNNNNNNGC 3 cut(s) 614, 755, 975
BstPAI GACNNNNGTC 1 cut(s) 1040
BstSCI CCNGG 1 cut(s) 145
BstSFI CTRYAG 2 cut(s) 171, 320
BstSLI GKGCMC 1 cut(s) 971
BstV1I GCAGC 7 cut(s) 61, 64, 334, 473, 770, 902, 987
BstV2I GAAGAC 1 cut(s) 336
BstX2I RGATCY 3 cut(s) 142, 310, 598
BstYI RGATCY 3 cut(s) 142, 310, 598
BstZ17I GTATAC 1 cut(s) 677
BsuRI GGCC 1 cut(s) 608
BtgI CCRYGG 1 cut(s) 1047
BtsCI GGATG 2 cut(s) 867, 1012
BtsI GCAGTG 1 cut(s) 120
BtsIMutI CAGTG 2 cut(s) 120, 1017
BveI ACCTGC 1 cut(s) 327
Cac8I GCNNGC 1 cut(s) 763
Cfr10I RCCGGY 1 cut(s) 298
Csp6I GTAC 3 cut(s) 442, 506, 951
CviAII CATG 2 cut(s) 604, 1048
CviQI GTAC 3 cut(s) 442, 506, 951
DdeI CTNAG 2 cut(s) 256, 1020
DpnI GATC 7 cut(s) 144, 312, 378, 420, 600, 662, 981
DpnII GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
DraIII CACNNNGTG 1 cut(s) 514
EaeI YGGCCR 1 cut(s) 606
Eam1104I CTCTTC 4 cut(s) 241, 475, 773, 790
EarI CTCTTC 4 cut(s) 241, 475, 773, 790
Eco130I CCWWGG 1 cut(s) 1047
Eco24I GRGCYC 1 cut(s) 572
Eco32I GATATC 1 cut(s) 844
Eco57I CTGAAG 1 cut(s) 291
EcoRV GATATC 1 cut(s) 844
EcoT14I CCWWGG 1 cut(s) 1047
EcoT38I GRGCYC 1 cut(s) 572
ErhI CCWWGG 1 cut(s) 1047
FaeI CATG 2 cut(s) 607, 1051
FalI AAGNNNNNCTT 2 cut(s) 321, 353
FaqI GGGAC 5 cut(s) 218, 236, 784, 1016, 1028
FatI CATG 2 cut(s) 603, 1047
FblI GTMKAC 2 cut(s) 676, 834
Fnu4HI GCNGC 7 cut(s) 75, 78, 323, 462, 759, 916, 976
FokI GGATG 2 cut(s) 854, 999
FriOI GRGCYC 1 cut(s) 572
Fsp4HI GCNGC 7 cut(s) 75, 78, 323, 462, 759, 916, 976
FspBI CTAG 1 cut(s) 762
GluI GCNGC 7 cut(s) 75, 78, 323, 462, 759, 916, 976
GsuI CTGGAG 1 cut(s) 234
HaeIII GGCC 1 cut(s) 608
HapII CCGG 4 cut(s) 110, 147, 228, 299
Hin1II CATG 2 cut(s) 607, 1051
HincII GTYRAC 1 cut(s) 166
HindII GTYRAC 1 cut(s) 166
HindIII AAGCTT 1 cut(s) 812
HinfI GANTC 4 cut(s) 131, 249, 282, 797
HpaII CCGG 4 cut(s) 110, 147, 228, 299
HphI GGTGA 2 cut(s) 4, 913
Hpy166II GTNNAC 4 cut(s) 166, 508, 677, 835
Hpy188I TCNGA 5 cut(s) 259, 315, 418, 665, 1082
Hpy188III TCNNGA 4 cut(s) 286, 308, 565, 818
Hpy8I GTNNAC 4 cut(s) 166, 508, 677, 835
HpyAV CCTTC 3 cut(s) 225, 888, 1018
HpyCH4III ACNGT 1 cut(s) 1007
HpyCH4IV ACGT 2 cut(s) 104, 510
HpyCH4V TGCA 6 cut(s) 183, 322, 461, 519, 539, 975
HpyF10VI GCNNNNNNNGC 3 cut(s) 614, 755, 975
HpyF3I CTNAG 2 cut(s) 256, 1020
HpySE526I ACGT 2 cut(s) 104, 510
Hsp92II CATG 2 cut(s) 607, 1051
Kzo9I GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
LmnI GCTCC 2 cut(s) 567, 650
Lsp1109I GCAGC 7 cut(s) 61, 64, 334, 473, 770, 902, 987
LweI GCATC 1 cut(s) 448
MaeI CTAG 1 cut(s) 762
MaeII ACGT 2 cut(s) 104, 510
MaeIII GTNAC 3 cut(s) 49, 100, 1007
MalI GATC 7 cut(s) 144, 312, 378, 420, 600, 662, 981
MboI GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
MboII GAAGA 7 cut(s) 258, 284, 301, 341, 492, 760, 777
MfeI CAATTG 1 cut(s) 684
MflI RGATCY 3 cut(s) 142, 310, 598
MhlI GDGCHC 2 cut(s) 572, 971
MlsI TGGCCA 1 cut(s) 608
MluCI AATT 7 cut(s) 4, 422, 486, 610, 684, 1065, 1073
MluNI TGGCCA 1 cut(s) 608
MlyI GAGTC 2 cut(s) 258, 806
Mox20I TGGCCA 1 cut(s) 608
MscI TGGCCA 1 cut(s) 608
MseI TTAA 1 cut(s) 83
Msp20I TGGCCA 1 cut(s) 608
MspI CCGG 4 cut(s) 110, 147, 228, 299
MspR9I CCNGG 1 cut(s) 147
MunI CAATTG 1 cut(s) 684
Mva1269I GAATGC 1 cut(s) 942
MwoI GCNNNNNNNGC 3 cut(s) 614, 755, 975
NciI CCSGG 1 cut(s) 147
NcoI CCATGG 1 cut(s) 1047
NdeII GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
NheI GCTAGC 1 cut(s) 761
NlaIII CATG 2 cut(s) 607, 1051
NlaIV GGNNCC 3 cut(s) 144, 312, 569
PctI GAATGC 1 cut(s) 942
PfeI GAWTC 2 cut(s) 131, 282
PflMI CCANNNNNTGG 1 cut(s) 686
PfoI TCCNGGA 1 cut(s) 145
PkrI GCNGC 7 cut(s) 76, 79, 324, 463, 760, 917, 977
PleI GAGTC 2 cut(s) 257, 805
PpsI GAGTC 2 cut(s) 257, 805
PshAI GACNNNNGTC 1 cut(s) 1040
Psp1406I AACGTT 1 cut(s) 104
PspN4I GGNNCC 3 cut(s) 144, 312, 569
PstI CTGCAG 1 cut(s) 324
PsuI RGATCY 3 cut(s) 142, 310, 598
RsaI GTAC 3 cut(s) 443, 507, 952
RsaNI GTAC 3 cut(s) 442, 506, 951
SaqAI TTAA 1 cut(s) 83
SatI GCNGC 7 cut(s) 75, 78, 323, 462, 759, 916, 976
Sau3AI GATC 7 cut(s) 142, 310, 376, 418, 598, 660, 979
SchI GAGTC 2 cut(s) 258, 806
ScrFI CCNGG 1 cut(s) 147
SduI GDGCHC 2 cut(s) 572, 971
SfaNI GCATC 1 cut(s) 448
SfcI CTRYAG 2 cut(s) 171, 320
Sse9I AATT 7 cut(s) 4, 422, 486, 610, 684, 1065, 1073
SsiI CCGC 1 cut(s) 918
SspMI CTAG 1 cut(s) 762
StyD4I CCNGG 1 cut(s) 145
StyI CCWWGG 1 cut(s) 1047
TaaI ACNGT 1 cut(s) 1007
TaiI ACGT 2 cut(s) 107, 513
TaqI TCGA 3 cut(s) 754, 986, 1056
TasI AATT 7 cut(s) 4, 422, 486, 610, 684, 1065, 1073
TatI WGTACW 3 cut(s) 441, 505, 950
TfiI GAWTC 2 cut(s) 131, 282
Tru1I TTAA 1 cut(s) 83
Tru9I TTAA 1 cut(s) 83
TscAI CASTG 2 cut(s) 120, 1024
TseI GCWGC 7 cut(s) 74, 77, 322, 461, 758, 915, 975
TspDTI ATGAA 2 cut(s) 17, 46
TspGWI ACGGA 1 cut(s) 258
TspRI CASTG 2 cut(s) 120, 1024
Van91I CCANNNNNTGG 1 cut(s) 686
XapI RAATTY 2 cut(s) 4, 1065
XmiI GTMKAC 2 cut(s) 676, 834
XspI CTAG 1 cut(s) 762
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.