pycom15g00720

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0000393
Physical Location & Seq
Reverse (-)
394075 .. 395560
1486 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g00720.4

Sequence Viewer

Length: 1080 bp
ATGACCAGTTTATTTTTCTTTTTGCTTGGTTCTCTAGACCTGATATTCTCTACTGCCCAAGCAACTGTGAAGCTACCAAAAAATGTTACAATTCCTGCTGTTTTTATGTTCGGGGATTCAATCGTTGATACAGGCAACAACAACAACCTTGTAACAATTATTAAAAGCAATTTTCCCCCATATGGAAGAGATTTTGTGGGAGGAGTGGCTACCGGAAGATTTGGCAATGGCAAGGTGCCCTCAGACATCGTTGTGGAGGAACTGGGAATCAAAAAACTTTTGCCGGCGTATCTTGATCCAAGTCTCCAAGAGAAGGACTTTGCCACCGGAGTAAACTTTGCTTCAGGCGCTTCAGGATTCGATCCATTAACATCTGAGATGATGTCTGTTATACCGCTATCGGAACAATTACTACTGCTGGAAGAATACAAAGAGAAGCTGAAAAATTATGTTGGAGGAAAGAGAGCAAAGAGCATTGTAAGCAAGAGCCTACACTTTGTGGTAACAGGCAGTGACGACCTAGTGAATACCTACTTTCATACACCTGCACGCTCCTTGCAATACAATATAGATGCCTACATTGATTTTATGGTGGCCGAGGCTTCAGCCTTCGTGCGGGAATTATATGCATTGGGAGCACGGAGGATTGTCATTTCTGGCCTACCACCGGTTGGATGTTTGCCATCAATGAGAACTGTAGACGGAGGTTCAGAAAGAAATTGTGTTGCGAGATACAATCAAGCAGCAGAGTTGTTCAACTCCAAGCTATCCGTGGAGGTGGACCGCCTTAACAAGCAGCTATTCCATGCCAAAGTGGTGGTTTTGATGGATGTCTACGGTCCATTCATGGATATCATCCTCAAGCCACAAAAATATGGATTTAAAGTTGAAGACAAAGGTTGTTGTGGAACAGGAAGAATAGAGGTTGTCAGATTGTGCAATCGATTGTCTCCAAACACATGCAACAATACCAGAGAGTACGTTTTCTGGGACAGTTATCATCCAACTGAAAGAGTATACAAGTTATTTGTCCCAAAGTTACTCCAAAAGTGCATTAACGACTTATTCAGCCAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

360

Amino Acids

39.94

Weight (kDa)

8.54

Isoelectric Point (pI)

25.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 553
Acc36I ACCTGC 1 cut(s) 553
AccB1I GGYRCC 1 cut(s) 235
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 3 cut(s) 699, 834, 1017
AciI CCGC 3 cut(s) 395, 616, 784
AclWI GGATC 2 cut(s) 290, 356
AcoI YGGCCR 1 cut(s) 594
AcuI CTGAAG 3 cut(s) 327, 336, 588
AdeI CACNNNGTG 1 cut(s) 499
AfaI GTAC 1 cut(s) 980
AfiI CCNNNNNNNGG 5 cut(s) 182, 313, 615, 667, 671
AgeI ACCGGT 1 cut(s) 667
AgsI TTSAA 3 cut(s) 120, 757, 890
AluBI AGCT 4 cut(s) 73, 439, 766, 799
AluI AGCT 4 cut(s) 73, 439, 766, 799
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 2 cut(s) 308, 954
AlwI GGATC 2 cut(s) 290, 356
AoxI GGCC 2 cut(s) 594, 658
ApeKI GCWGC 2 cut(s) 743, 796
AsiGI ACCGGT 1 cut(s) 667
AspLEI GCGC 1 cut(s) 350
AspS9I GGNCC 2 cut(s) 781, 839
AvaII GGWCC 2 cut(s) 781, 839
BaeGI GKGCMC 1 cut(s) 240
BanI GGYRCC 1 cut(s) 235
BbsI GAAGAC 1 cut(s) 897
Bbv12I GWGCWC 1 cut(s) 640
BbvI GCAGC 2 cut(s) 755, 808
BccI CCATC 2 cut(s) 691, 820
BcoDI GTCTC 2 cut(s) 308, 954
BfaI CTAG 2 cut(s) 35, 521
BfmI CTRYAG 1 cut(s) 696
BfoI RGCGCY 1 cut(s) 351
BfuAI ACCTGC 1 cut(s) 553
BisI GCNGC 2 cut(s) 744, 797
BlsI GCNGC 2 cut(s) 745, 798
Bme18I GGWCC 2 cut(s) 781, 839
BmgT120I GGNCC 2 cut(s) 781, 839
BmiI GGNNCC 1 cut(s) 237
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 1 cut(s) 562
BmuI ACTGGG 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 897
BpuEI CTTGAG 1 cut(s) 845
Bsa29I ATCGAT 1 cut(s) 943
BsaJI CCNNGG 2 cut(s) 597, 771
BsaWI WCCGGW 3 cut(s) 212, 326, 667
Bsc4I CCNNNNNNNGG 5 cut(s) 182, 313, 615, 667, 671
Bse118I RCCGGY 2 cut(s) 283, 667
Bse1I ACTGG 2 cut(s) 6, 267
Bse3DI GCAATG 1 cut(s) 232
BseCI ATCGAT 1 cut(s) 943
BseDI CCNNGG 2 cut(s) 597, 771
BseGI GGATG 4 cut(s) 680, 835, 855, 1000
BseLI CCNNNNNNNGG 5 cut(s) 182, 313, 615, 667, 671
BseMI GCAATG 1 cut(s) 232
BseMII CTCAG 2 cut(s) 255, 366
BseNI ACTGG 2 cut(s) 6, 267
BseRI GAGGAG 1 cut(s) 216
BseSI GKGCMC 1 cut(s) 240
BseXI GCAGC 2 cut(s) 755, 808
BsgI GTGCAG 1 cut(s) 531
BshFI GGCC 2 cut(s) 596, 660
BshNI GGYRCC 1 cut(s) 235
BshTI ACCGGT 1 cut(s) 667
BshVI ATCGAT 1 cut(s) 943
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 4 cut(s) 213, 284, 327, 668
BslFI GGGAC 2 cut(s) 1004, 1016
BslI CCNNNNNNNGG 5 cut(s) 182, 313, 615, 667, 671
BsmAI GTCTC 2 cut(s) 308, 954
BsmFI GGGAC 2 cut(s) 1004, 1016
BsnI GGCC 2 cut(s) 596, 660
Bsp1286I GDGCHC 2 cut(s) 240, 640
Bsp143I GATC 2 cut(s) 295, 361
BspACI CCGC 3 cut(s) 395, 616, 784
BspANI GGCC 2 cut(s) 596, 660
BspCNI CTCAG 2 cut(s) 254, 367
BspDI ATCGAT 1 cut(s) 943
BspLI GGNNCC 1 cut(s) 237
BspMI ACCTGC 1 cut(s) 553
BspPI GGATC 2 cut(s) 290, 356
BspT107I GGYRCC 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 232
BsrFI RCCGGY 2 cut(s) 283, 667
BsrI ACTGG 2 cut(s) 6, 267
BssAI RCCGGY 2 cut(s) 283, 667
BssECI CCNNGG 2 cut(s) 597, 771
BssMI GATC 2 cut(s) 295, 361
BssNAI GTATAC 1 cut(s) 1018
Bst1107I GTATAC 1 cut(s) 1018
Bst4CI ACNGT 4 cut(s) 67, 697, 839, 995
Bst6I CTCTTC 1 cut(s) 181
BstC8I GCNNGC 2 cut(s) 285, 550
BstDEI CTNAG 2 cut(s) 241, 375
BstDSI CCRYGG 1 cut(s) 771
BstF5I GGATG 4 cut(s) 680, 835, 855, 1000
BstH2I RGCGCY 1 cut(s) 351
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 2 cut(s) 298, 364
BstMAI GTCTC 2 cut(s) 308, 954
BstMBI GATC 2 cut(s) 295, 361
BstMWI GCNNNNNNNGC 3 cut(s) 347, 480, 635
BstNSI RCATGY 1 cut(s) 963
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 240
BstV1I GCAGC 2 cut(s) 755, 808
BstV2I GAAGAC 1 cut(s) 897
BstXI CCANNNNNNTGG 1 cut(s) 817
BstZ17I GTATAC 1 cut(s) 1018
Bsu15I ATCGAT 1 cut(s) 943
BsuRI GGCC 2 cut(s) 596, 660
BsuTUI ATCGAT 1 cut(s) 943
BtgI CCRYGG 1 cut(s) 771
BtsCI GGATG 4 cut(s) 680, 835, 855, 1000
BtsI GCAGTG 1 cut(s) 517
BtsIMutI CAGTG 1 cut(s) 517
BveI ACCTGC 1 cut(s) 553
Cac8I GCNNGC 2 cut(s) 285, 550
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 2 cut(s) 283, 667
Cfr13I GGNCC 2 cut(s) 781, 839
ClaI ATCGAT 1 cut(s) 943
Csp6I GTAC 1 cut(s) 979
CspAI ACCGGT 1 cut(s) 667
CviAII CATG 3 cut(s) 806, 847, 960
CviQI GTAC 1 cut(s) 979
DdeI CTNAG 2 cut(s) 241, 375
DpnI GATC 2 cut(s) 297, 363
DpnII GATC 2 cut(s) 295, 361
DraI TTTAAA 1 cut(s) 883
DraIII CACNNNGTG 1 cut(s) 499
EaeI YGGCCR 1 cut(s) 594
Eam1104I CTCTTC 1 cut(s) 181
EarI CTCTTC 1 cut(s) 181
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 2 cut(s) 781, 839
Eco57I CTGAAG 3 cut(s) 327, 336, 588
EcoRV GATATC 1 cut(s) 853
EcoT22I ATGCAT 1 cut(s) 631
FaeI CATG 3 cut(s) 809, 850, 963
FaqI GGGAC 2 cut(s) 1004, 1016
FatI CATG 3 cut(s) 805, 846, 959
FauI CCCGC 1 cut(s) 609
FauNDI CATATG 1 cut(s) 181
FblI GTMKAC 3 cut(s) 699, 834, 1017
Fnu4HI GCNGC 2 cut(s) 744, 797
FokI GGATG 4 cut(s) 687, 842, 842, 987
Fsp4HI GCNGC 2 cut(s) 744, 797
FspBI CTAG 2 cut(s) 35, 521
GlaI GCGC 1 cut(s) 349
GluI GCNGC 2 cut(s) 744, 797
HaeII RGCGCY 1 cut(s) 351
HaeIII GGCC 2 cut(s) 596, 660
HapII CCGG 4 cut(s) 213, 284, 327, 668
HhaI GCGC 1 cut(s) 350
Hin1II CATG 3 cut(s) 809, 850, 963
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HinfI GANTC 3 cut(s) 116, 267, 357
HpaII CCGG 4 cut(s) 213, 284, 327, 668
Hpy166II GTNNAC 5 cut(s) 334, 700, 781, 835, 1018
Hpy188I TCNGA 5 cut(s) 244, 376, 403, 712, 932
Hpy188III TCNNGA 3 cut(s) 35, 293, 354
Hpy8I GTNNAC 5 cut(s) 334, 700, 781, 835, 1018
HpyAV CCTTC 2 cut(s) 307, 619
HpyCH4III ACNGT 4 cut(s) 67, 697, 839, 995
HpyCH4IV ACGT 1 cut(s) 981
HpyCH4V TGCA 6 cut(s) 548, 559, 629, 939, 963, 1053
HpyF10VI GCNNNNNNNGC 3 cut(s) 347, 480, 635
HpyF3I CTNAG 2 cut(s) 241, 375
HpySE526I ACGT 1 cut(s) 981
Hsp92II CATG 3 cut(s) 809, 850, 963
HspAI GCGC 1 cut(s) 348
KroI GCCGGC 1 cut(s) 283
KroNI GCCGGC 1 cut(s) 285
Kzo9I GATC 2 cut(s) 295, 361
LmnI GCTCC 2 cut(s) 557, 635
Lsp1109I GCAGC 2 cut(s) 755, 808
LweI GCATC 1 cut(s) 562
MaeI CTAG 2 cut(s) 35, 521
MaeII ACGT 1 cut(s) 981
MaeIII GTNAC 5 cut(s) 85, 151, 502, 512, 1038
MalI GATC 2 cut(s) 297, 363
MboI GATC 2 cut(s) 295, 361
MboII GAAGA 5 cut(s) 198, 228, 434, 902, 927
MhlI GDGCHC 2 cut(s) 240, 640
MluCI AATT 7 cut(s) 90, 156, 169, 407, 445, 620, 718
MmeI TCCRAC 3 cut(s) 433, 652, 1028
Mph1103I ATGCAT 1 cut(s) 631
MroNI GCCGGC 1 cut(s) 283
MseI TTAA 5 cut(s) 162, 368, 789, 882, 1056
MslI CAYNNNNRTG 1 cut(s) 251
MspI CCGG 4 cut(s) 213, 284, 327, 668
MwoI GCNNNNNNNGC 3 cut(s) 347, 480, 635
NaeI GCCGGC 1 cut(s) 285
NdeI CATATG 1 cut(s) 181
NdeII GATC 2 cut(s) 295, 361
NgoMIV GCCGGC 1 cut(s) 283
NlaIII CATG 3 cut(s) 809, 850, 963
NlaIV GGNNCC 1 cut(s) 237
NmeAIII GCCGAG 1 cut(s) 622
NmuCI GTSAC 1 cut(s) 512
NsiI ATGCAT 1 cut(s) 631
NspI RCATGY 1 cut(s) 963
PaqCI CACCTGC 1 cut(s) 553
PdiI GCCGGC 1 cut(s) 285
PfeI GAWTC 3 cut(s) 116, 267, 357
PflMI CCANNNNNTGG 1 cut(s) 671
PinAI ACCGGT 1 cut(s) 667
PkrI GCNGC 2 cut(s) 745, 798
PspN4I GGNNCC 1 cut(s) 237
PspPI GGNCC 2 cut(s) 781, 839
PsrI GAACNNNNNNTAC 2 cut(s) 396, 428
RsaI GTAC 1 cut(s) 980
RsaNI GTAC 1 cut(s) 979
RseI CAYNNNNRTG 1 cut(s) 251
SaqAI TTAA 5 cut(s) 162, 368, 789, 882, 1056
SatI GCNGC 2 cut(s) 744, 797
Sau3AI GATC 2 cut(s) 295, 361
Sau96I GGNCC 2 cut(s) 781, 839
SduI GDGCHC 2 cut(s) 240, 640
SfaNI GCATC 1 cut(s) 562
SfcI CTRYAG 1 cut(s) 696
SinI GGWCC 2 cut(s) 781, 839
SmiMI CAYNNNNRTG 1 cut(s) 251
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
Sse9I AATT 7 cut(s) 90, 156, 169, 407, 445, 620, 718
SsiI CCGC 3 cut(s) 395, 616, 784
SspMI CTAG 2 cut(s) 35, 521
TaaI ACNGT 4 cut(s) 67, 697, 839, 995
TaiI ACGT 1 cut(s) 984
TaqI TCGA 2 cut(s) 360, 943
TasI AATT 7 cut(s) 90, 156, 169, 407, 445, 620, 718
TfiI GAWTC 3 cut(s) 116, 267, 357
Tru1I TTAA 5 cut(s) 162, 368, 789, 882, 1056
Tru9I TTAA 5 cut(s) 162, 368, 789, 882, 1056
TscAI CASTG 1 cut(s) 517
TseFI GTSAC 1 cut(s) 512
TseI GCWGC 2 cut(s) 743, 796
Tsp45I GTSAC 1 cut(s) 512
TspDTI ATGAA 2 cut(s) 527, 835
TspGWI ACGGA 3 cut(s) 655, 717, 760
TspRI CASTG 1 cut(s) 517
Van91I CCANNNNNTGG 1 cut(s) 671
VpaK11BI GGWCC 2 cut(s) 781, 839
XbaI TCTAGA 1 cut(s) 34
XceI RCATGY 1 cut(s) 963
XcmI CCANNNNNNNNNTGG 1 cut(s) 769
XmiI GTMKAC 3 cut(s) 699, 834, 1017
XspI CTAG 2 cut(s) 35, 521
Zsp2I ATGCAT 1 cut(s) 631
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.