Rw2G027650

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000393
Physical Location & Seq
Forward (+)
43649767 .. 43654816
5050 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027650.1

Sequence Viewer

Length: 1068 bp
ATGATCTTATGTATATTCCTCTCCCATAACGTTGCTGCTGTAACACCTCCCATAAATGAAACAACTCGGGCAGTCTTTACTTTCGGAGATTCAACATTAGATACCAGCAATAATGACTACATCATTTGTATAATCAAAAGCAATTTCTCACCTTATGGGAGAGACTTTATGGGAGGAAAGCCTATCGGAAGATTTAGCAATGGAAGAGTCCCATCAGACTTCATTGGTGAAGGATTTGGATTGAAGAAGATTTTGCCGGCTTATCTGGATCCGAATTTGCAGCTTCAAGACCTACTTACTGGTGTATGTTTTGCCTCAGCAGGTTCAGGATATGACCCTCTTACTGCCAAATCACTGGTACACACACACACACATACATACATATATATAACATATCATATCGTATCATATCATACATCCTTGGTTTCTCTTAATTTCATTTGTATCAGTTTAAAAAAAAAAAAATCAGATCAATTAAATTTGTTCAAAGAATACAAAAGCAAGATAAACGCAGTAGTTAGGAATGAGAGAACAGCCACTCTACTATCAAAAAGCTTATTTGTTCTTTCGATAGGAAGCAATGGACTTGCACTTAATTATTTTTCTACACCACTGAGGAGCGCTCACTATGATATTCCAGCTTATACTGAACTTTATGCACTGGAAGCAAGAGTGATTGAAGTAGCAAGTATGCCGCCAATTGGGTGTGTACCAGCACAGAGAACACTAGGTGGTGCCATAGAGAGAGCCTGCGATGAGACTGAGAACCAAGTAGCAATCCTCTTTAACTCGAAGCTCTCCGCCCTTATAAACTCCCTCAATAAGAGACTTCCAGAAGCACAACTCATTTACGTTGACATATATAACCCTTTGTTGTCCATTATCCAGGAGCCTGCTCAAAATGTGGTGGATAGAGGATGTTGTGGAACAGGAACTGTTGAGTTCGGGTCTCTGTGTAACAAAGAGACCCAGAGTACTTGCAACGATGCGTCCAAGTACATATTCTGGGATGGCCTTCATCCTATAGAAATAGCTTACAGGATCCTCACCTATGCAATATTAAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

39.21

Weight (kDa)

7.54

Isoelectric Point (pI)

34.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 25 - 350 1.2e-23 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 809
Acc36I ACCTGC 1 cut(s) 311
AccB1I GGYRCC 1 cut(s) 734
AciI CCGC 2 cut(s) 695, 801
AclI AACGTT 1 cut(s) 30
AclWI GGATC 4 cut(s) 263, 276, 1036, 1049
AcsI RAATTY 2 cut(s) 274, 478
AdeI CACNNNGTG 1 cut(s) 731
AfaI GTAC 4 cut(s) 360, 711, 976, 998
AfeI AGCGCT 1 cut(s) 622
AfiI CCNNNNNNNGG 1 cut(s) 701
AgsI TTSAA 5 cut(s) 93, 244, 287, 487, 680
AjnI CCWGG 1 cut(s) 885
AjuI GAANNNNNNNTTGG 2 cut(s) 986, 1018
AluBI AGCT 5 cut(s) 283, 555, 641, 796, 1034
AluI AGCT 5 cut(s) 283, 555, 641, 796, 1034
Alw26I GTCTC 5 cut(s) 156, 752, 820, 954, 959
AlwI GGATC 4 cut(s) 263, 276, 1036, 1049
AlwNI CAGNNNCTG 1 cut(s) 935
Ama87I CYCGRG 1 cut(s) 66
Aor51HI AGCGCT 1 cut(s) 622
AoxI GGCC 1 cut(s) 1012
ApeKI GCWGC 2 cut(s) 35, 280
ApoI RAATTY 2 cut(s) 274, 478
AspLEI GCGC 1 cut(s) 623
AsuHPI GGTGA 3 cut(s) 141, 239, 1039
AvaI CYCGRG 1 cut(s) 66
BamHI GGATCC 2 cut(s) 268, 1041
BanI GGYRCC 1 cut(s) 734
BbvCI CCTCAGC 1 cut(s) 316
BbvI GCAGC 2 cut(s) 22, 292
BccI CCATC 2 cut(s) 220, 1004
BciT130I CCWGG 1 cut(s) 887
BcoDI GTCTC 5 cut(s) 156, 752, 820, 954, 959
BfaI CTAG 1 cut(s) 728
BfmI CTRYAG 1 cut(s) 1023
BfoI RGCGCY 1 cut(s) 624
BfuAI ACCTGC 1 cut(s) 311
BisI GCNGC 3 cut(s) 36, 281, 695
BlsI GCNGC 3 cut(s) 37, 282, 696
BmcAI AGTACT 1 cut(s) 976
Bme1390I CCNGG 1 cut(s) 887
BmeT110I CYCGRG 1 cut(s) 66
BmiI GGNNCC 4 cut(s) 270, 736, 891, 1043
BmrFI CCNGG 1 cut(s) 887
BmsI GCATC 1 cut(s) 976
BplI GAGNNNNNCTC 2 cut(s) 607, 639
Bpu10I CCTNAGC 1 cut(s) 316
BsaI GGTCTC 2 cut(s) 954, 959
BsaJI CCNNGG 1 cut(s) 420
Bsc4I CCNNNNNNNGG 1 cut(s) 701
Bse118I RCCGGY 1 cut(s) 256
Bse1I ACTGG 3 cut(s) 304, 360, 666
Bse3DI GCAATG 2 cut(s) 205, 586
BseBI CCWGG 1 cut(s) 887
BseDI CCNNGG 1 cut(s) 420
BseGI GGATG 4 cut(s) 416, 923, 1015, 1018
BseLI CCNNNNNNNGG 1 cut(s) 701
BseMI GCAATG 2 cut(s) 205, 586
BseMII CTCAG 3 cut(s) 330, 605, 753
BseNI ACTGG 3 cut(s) 304, 360, 666
BseRI GAGGAG 1 cut(s) 631
BseXI GCAGC 2 cut(s) 22, 292
BshFI GGCC 1 cut(s) 1014
BshNI GGYRCC 1 cut(s) 734
BsiHKCI CYCGRG 1 cut(s) 66
BsiSI CCGG 1 cut(s) 257
BslFI GGGAC 1 cut(s) 194
BslI CCNNNNNNNGG 1 cut(s) 701
BsmAI GTCTC 5 cut(s) 156, 752, 820, 954, 959
BsmFI GGGAC 1 cut(s) 194
BsnI GGCC 1 cut(s) 1014
Bso31I GGTCTC 2 cut(s) 954, 959
BsoBI CYCGRG 1 cut(s) 66
Bsp143I GATC 4 cut(s) 3, 268, 469, 1041
BspACI CCGC 2 cut(s) 695, 801
BspANI GGCC 1 cut(s) 1014
BspCNI CTCAG 3 cut(s) 329, 606, 754
BspLI GGNNCC 4 cut(s) 270, 736, 891, 1043
BspMI ACCTGC 1 cut(s) 311
BspPI GGATC 4 cut(s) 263, 276, 1036, 1049
BspT107I GGYRCC 1 cut(s) 734
BspTNI GGTCTC 2 cut(s) 954, 959
BsrDI GCAATG 2 cut(s) 205, 586
BsrFI RCCGGY 1 cut(s) 256
BsrI ACTGG 3 cut(s) 304, 360, 666
BssAI RCCGGY 1 cut(s) 256
BssECI CCNNGG 1 cut(s) 420
BssMI GATC 4 cut(s) 3, 268, 469, 1041
BssT1I CCWWGG 1 cut(s) 420
Bst2UI CCWGG 1 cut(s) 887
Bst4CI ACNGT 1 cut(s) 937
Bst6I CTCTTC 1 cut(s) 199
BstC8I GCNNGC 3 cut(s) 258, 751, 894
BstDEI CTNAG 3 cut(s) 316, 614, 762
BstF5I GGATG 4 cut(s) 416, 923, 1015, 1018
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 4 cut(s) 6, 271, 472, 1044
BstMAI GTCTC 5 cut(s) 156, 752, 820, 954, 959
BstMBI GATC 4 cut(s) 3, 268, 469, 1041
BstMWI GCNNNNNNNGC 1 cut(s) 665
BstNI CCWGG 1 cut(s) 887
BstSCI CCNGG 1 cut(s) 885
BstSFI CTRYAG 1 cut(s) 1023
BstV1I GCAGC 2 cut(s) 22, 292
BstX2I RGATCY 2 cut(s) 268, 1041
BstXI CCANNNNNNTGG 1 cut(s) 355
BstYI RGATCY 2 cut(s) 268, 1041
BsuRI GGCC 1 cut(s) 1014
BtgZI GCGATG 1 cut(s) 768
BtsCI GGATG 4 cut(s) 416, 923, 1015, 1018
BtsIMutI CAGTG 3 cut(s) 353, 611, 659
BveI ACCTGC 1 cut(s) 311
Cac8I GCNNGC 3 cut(s) 258, 751, 894
CaiI CAGNNNCTG 1 cut(s) 935
CfoI GCGC 1 cut(s) 623
Cfr10I RCCGGY 1 cut(s) 256
CseI GACGC 1 cut(s) 978
Csp6I GTAC 4 cut(s) 359, 710, 975, 997
CviQI GTAC 4 cut(s) 359, 710, 975, 997
DdeI CTNAG 3 cut(s) 316, 614, 762
DpnI GATC 4 cut(s) 5, 270, 471, 1043
DpnII GATC 4 cut(s) 3, 268, 469, 1041
DraI TTTAAA 1 cut(s) 453
DraIII CACNNNGTG 1 cut(s) 731
Eam1104I CTCTTC 1 cut(s) 199
EarI CTCTTC 1 cut(s) 199
EciI GGCGGA 1 cut(s) 790
Eco130I CCWWGG 1 cut(s) 420
Eco31I GGTCTC 2 cut(s) 954, 959
Eco47III AGCGCT 1 cut(s) 622
Eco88I CYCGRG 1 cut(s) 66
EcoRII CCWGG 1 cut(s) 885
EcoT14I CCWWGG 1 cut(s) 420
ErhI CCWWGG 1 cut(s) 420
FalI AAGNNNNNCTT 2 cut(s) 279, 311
FaqI GGGAC 1 cut(s) 194
Fnu4HI GCNGC 3 cut(s) 36, 281, 695
FokI GGATG 4 cut(s) 403, 930, 1005, 1022
Fsp4HI GCNGC 3 cut(s) 36, 281, 695
FspBI CTAG 1 cut(s) 728
GlaI GCGC 1 cut(s) 622
GluI GCNGC 3 cut(s) 36, 281, 695
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 1 cut(s) 1014
HapII CCGG 1 cut(s) 257
HgaI GACGC 1 cut(s) 978
HhaI GCGC 1 cut(s) 623
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HincII GTYRAC 1 cut(s) 856
HindII GTYRAC 1 cut(s) 856
HindIII AAGCTT 1 cut(s) 553
HinfI GANTC 2 cut(s) 89, 207
HpaII CCGG 1 cut(s) 257
HphI GGTGA 3 cut(s) 141, 239, 1039
Hpy166II GTNNAC 3 cut(s) 361, 710, 856
Hpy188I TCNGA 5 cut(s) 86, 188, 217, 273, 469
Hpy188III TCNNGA 4 cut(s) 266, 287, 327, 833
Hpy8I GTNNAC 3 cut(s) 361, 710, 856
HpyAV CCTTC 2 cut(s) 224, 1025
HpyCH4III ACNGT 1 cut(s) 937
HpyCH4IV ACGT 2 cut(s) 30, 852
HpyCH4V TGCA 5 cut(s) 280, 590, 659, 981, 1055
HpyF10VI GCNNNNNNNGC 1 cut(s) 665
HpyF3I CTNAG 3 cut(s) 316, 614, 762
HpySE526I ACGT 2 cut(s) 30, 852
HspAI GCGC 1 cut(s) 621
KroI GCCGGC 1 cut(s) 256
KroNI GCCGGC 1 cut(s) 258
Kzo9I GATC 4 cut(s) 3, 268, 469, 1041
LmnI GCTCC 2 cut(s) 618, 889
Lsp1109I GCAGC 2 cut(s) 22, 292
LweI GCATC 1 cut(s) 976
MaeI CTAG 1 cut(s) 728
MaeII ACGT 2 cut(s) 30, 852
MaeIII GTNAC 2 cut(s) 40, 956
MalI GATC 4 cut(s) 5, 270, 471, 1043
MboI GATC 4 cut(s) 3, 268, 469, 1041
MboII GAAGA 4 cut(s) 201, 216, 256, 259
MfeI CAATTG 1 cut(s) 699
MflI RGATCY 2 cut(s) 268, 1041
MluCI AATT 7 cut(s) 142, 274, 433, 473, 478, 595, 699
MlyI GAGTC 1 cut(s) 216
MroNI GCCGGC 1 cut(s) 256
MseI TTAA 6 cut(s) 432, 452, 476, 594, 786, 1061
MspI CCGG 1 cut(s) 257
MspR9I CCNGG 1 cut(s) 887
MunI CAATTG 1 cut(s) 699
MvaI CCWGG 1 cut(s) 887
MwoI GCNNNNNNNGC 1 cut(s) 665
NaeI GCCGGC 1 cut(s) 258
NdeII GATC 4 cut(s) 3, 268, 469, 1041
NgoMIV GCCGGC 1 cut(s) 256
NlaIV GGNNCC 4 cut(s) 270, 736, 891, 1043
PdiI GCCGGC 1 cut(s) 258
PfeI GAWTC 1 cut(s) 89
PfoI TCCNGGA 1 cut(s) 885
PkrI GCNGC 3 cut(s) 37, 282, 696
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
PsiI TTATAA 1 cut(s) 809
Psp1406I AACGTT 1 cut(s) 30
Psp6I CCWGG 1 cut(s) 885
PspGI CCWGG 1 cut(s) 885
PspN4I GGNNCC 4 cut(s) 270, 736, 891, 1043
PstNI CAGNNNCTG 1 cut(s) 935
PsuI RGATCY 2 cut(s) 268, 1041
RsaI GTAC 4 cut(s) 360, 711, 976, 998
RsaNI GTAC 4 cut(s) 359, 710, 975, 997
SaqAI TTAA 6 cut(s) 432, 452, 476, 594, 786, 1061
SatI GCNGC 3 cut(s) 36, 281, 695
Sau3AI GATC 4 cut(s) 3, 268, 469, 1041
ScaI AGTACT 1 cut(s) 976
SchI GAGTC 1 cut(s) 216
ScrFI CCNGG 1 cut(s) 887
SfaNI GCATC 1 cut(s) 976
SfcI CTRYAG 1 cut(s) 1023
Sse9I AATT 7 cut(s) 142, 274, 433, 473, 478, 595, 699
SsiI CCGC 2 cut(s) 695, 801
SspI AATATT 1 cut(s) 1059
SspMI CTAG 1 cut(s) 728
StyD4I CCNGG 1 cut(s) 885
StyI CCWWGG 1 cut(s) 420
TaaI ACNGT 1 cut(s) 937
TaiI ACGT 2 cut(s) 33, 855
TaqI TCGA 2 cut(s) 569, 791
TasI AATT 7 cut(s) 142, 274, 433, 473, 478, 595, 699
TatI WGTACW 2 cut(s) 974, 996
TauI GCSGC 1 cut(s) 697
TfiI GAWTC 1 cut(s) 89
Tru1I TTAA 6 cut(s) 432, 452, 476, 594, 786, 1061
Tru9I TTAA 6 cut(s) 432, 452, 476, 594, 786, 1061
TscAI CASTG 3 cut(s) 360, 618, 666
TseI GCWGC 2 cut(s) 35, 280
TspDTI ATGAA 4 cut(s) 72, 211, 427, 1007
TspRI CASTG 3 cut(s) 360, 618, 666
XapI RAATTY 2 cut(s) 274, 478
XspI CTAG 1 cut(s) 728
ZrmI AGTACT 1 cut(s) 976
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.