AT1G20132

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
1
OG0000393
Physical Location & Seq
Forward (+)
6981264 .. 6983627
2364 bp
Loading structure...
UTR
Exon/CDS
Intron
AT1G20132.1

Sequence Viewer

Length: 1152 bp
ATGAAATCGATATTAATTGGATTTGTTTTCTTTCTTTTATCATCATTTCGTAGCTTCTTCGTGACCACTACCTATTCCCAAGTGATCCATCACCGACGTCTACGGCCATGGCCACCGCCAGAATCTGGGTCCGGCCCATCACCTGGCCCATCTCCATCACCACATAACAAAACCACACCGGCAGTGTTTTTCTTTGGGGATTCGATCATAGATACAGGAAATAATAATAATCTAACAACAGAAATGAAATGTAATTTTAGTCCTTATGGTAAGGATTTTCCACTCGGAGTTGCAACTGGTAGATTCAGCAACGGAAAGGTCGTTTCCGATTATATATCTGAATATTTGGGAGTAAAACCAATTGTACCAGCATATTTTGACCCCAATGTACAACTAGAAGATCTTCTCACGGGTGTATCATTTGCTTCAGGTGGTAGTGGCTATTACCATTTGACTCCCAAAATATCCAGAGTAAAGTCAATGTTGGAGCAATTGACATATTTCCAACGACACATAGCGAGAGTAAAGAGGTTAGTAGGGGAAGAGAAGACGGATCAACTGTTAGCCAAGGGCTTATCCGTTGTGGTCGCAGGCAGCAACGATTTGGCTATTACATATTATGGTCATGGTGCTCAATTGCTCAAAGATGATATCCACTACTTTACTTCAAAGATGGCAAATTCCGCGGCAAGTTTTGTTATGCAATTATACGAATATGGAGCAAGACAAATAGCAGTTCTAGGAACACCACCACTTGGCTGCGTACCAATACTAAGAACTCTAAAAGGAGGTCTTCGTAGAGAGTGTGCTCAAGATATAAACTATGCTTCTCAGCTTTTCAACGTCAAACTCTCCAACATTTTGGACCAATTGGCAAAAAACCTACCAAATTCCAATCTCATTTACATTGACATATACTCTGCTTTCAGTCACATCTTAGAAAATTCAGCAGATTATGGGTTTGAAGAAATAAAGAGAGGTTGTTGTGGAACTGGATTTGTGGAAGCTGGTCCACTTTGCAATCGATTCACAACATTTGTGTGCTCCAATGTATCTGCTTACATGTTTTGGGATAGTCTCCATCCTACCCAAAGATTCTATAAAATCTTAACCAAAATACTTTTTGAGAAATACATCCACAATTTAAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

383

Amino Acids

42.7

Weight (kDa)

8.9

Isoelectric Point (pI)

45.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 62 - 372 6.5e-36 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 100
AccB7I CCANNNNNTGG 3 cut(s) 125, 143, 755
AccI GTMKAC 1 cut(s) 100
AccII CGCG 1 cut(s) 686
AciI CCGC 3 cut(s) 116, 684, 686
AclWI GGATC 2 cut(s) 79, 561
AcoI YGGCCR 2 cut(s) 104, 110
AcsI RAATTY 3 cut(s) 679, 889, 943
AcuI CTGAAG 1 cut(s) 411
AcyI GRCGYC 1 cut(s) 97
AfaI GTAC 3 cut(s) 366, 390, 765
AfiI CCNNNNNNNGG 3 cut(s) 125, 143, 755
AflIII ACRYGT 1 cut(s) 1062
AgsI TTSAA 3 cut(s) 669, 841, 965
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 3 cut(s) 54, 835, 1007
AluI AGCT 3 cut(s) 54, 835, 1007
Alw21I GWGCWC 3 cut(s) 634, 811, 1046
Alw26I GTCTC 1 cut(s) 1082
AlwI GGATC 2 cut(s) 79, 561
AlwNI CAGNNNCTG 1 cut(s) 125
AoxI GGCC 4 cut(s) 104, 110, 133, 145
ApeKI GCWGC 2 cut(s) 594, 759
ApoI RAATTY 3 cut(s) 679, 889, 943
AseI ATTAAT 1 cut(s) 14
Asp700I GAANNNNTTC 1 cut(s) 402
AspS9I GGNCC 5 cut(s) 129, 134, 146, 865, 1010
AsuHPI GGTGA 3 cut(s) 83, 132, 150
AvaII GGWCC 3 cut(s) 129, 865, 1010
BaeI ACNNNNGTAYC 2 cut(s) 399, 432
BalI TGGCCA 1 cut(s) 112
BbsI GAAGAC 2 cut(s) 554, 785
Bbv12I GWGCWC 3 cut(s) 634, 811, 1046
BbvI GCAGC 2 cut(s) 606, 746
BccI CCATC 6 cut(s) 96, 145, 157, 163, 667, 1089
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 144
BcoDI GTCTC 1 cut(s) 1082
BfaI CTAG 2 cut(s) 395, 740
BglII AGATCT 1 cut(s) 400
BisI GCNGC 3 cut(s) 595, 687, 760
BlsI GCNGC 3 cut(s) 596, 688, 761
Bme1390I CCNGG 1 cut(s) 144
Bme18I GGWCC 3 cut(s) 129, 865, 1010
BmgT120I GGNCC 5 cut(s) 129, 134, 146, 865, 1010
BmiI GGNNCC 1 cut(s) 130
BmrFI CCNGG 1 cut(s) 144
BpiI GAAGAC 2 cut(s) 554, 785
BpuEI CTTGAG 1 cut(s) 795
Bsa29I ATCGAT 2 cut(s) 8, 1024
BsaHI GRCGYC 1 cut(s) 97
BsaJI CCNNGG 3 cut(s) 107, 567, 684
Bsc4I CCNNNNNNNGG 3 cut(s) 125, 143, 755
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 2 cut(s) 301, 997
BseBI CCWGG 1 cut(s) 144
BseCI ATCGAT 2 cut(s) 8, 1024
BseDI CCNNGG 3 cut(s) 107, 567, 684
BseGI GGATG 2 cut(s) 1081, 1134
BseLI CCNNNNNNNGG 3 cut(s) 125, 143, 755
BseMII CTCAG 1 cut(s) 845
BseNI ACTGG 2 cut(s) 301, 997
BseXI GCAGC 2 cut(s) 606, 746
Bsh1236I CGCG 1 cut(s) 686
BshFI GGCC 4 cut(s) 106, 112, 135, 147
BshVI ATCGAT 2 cut(s) 8, 1024
BsiHKAI GWGCWC 3 cut(s) 634, 811, 1046
BsiSI CCGG 2 cut(s) 132, 179
BslI CCNNNNNNNGG 3 cut(s) 125, 143, 755
BsmAI GTCTC 1 cut(s) 1082
BsnI GGCC 4 cut(s) 106, 112, 135, 147
Bsp1286I GDGCHC 3 cut(s) 634, 811, 1046
Bsp1407I TGTACA 1 cut(s) 388
Bsp143I GATC 4 cut(s) 84, 204, 400, 553
Bsp19I CCATGG 1 cut(s) 107
BspACI CCGC 3 cut(s) 116, 684, 686
BspANI GGCC 4 cut(s) 106, 112, 135, 147
BspCNI CTCAG 1 cut(s) 844
BspDI ATCGAT 2 cut(s) 8, 1024
BspFNI CGCG 1 cut(s) 686
BspLI GGNNCC 1 cut(s) 130
BspPI GGATC 2 cut(s) 79, 561
BsrFI RCCGGY 1 cut(s) 178
BsrGI TGTACA 1 cut(s) 388
BsrI ACTGG 2 cut(s) 301, 997
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 3 cut(s) 107, 567, 684
BssMI GATC 4 cut(s) 84, 204, 400, 553
BssNI GRCGYC 1 cut(s) 97
BssT1I CCWWGG 2 cut(s) 107, 567
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 1 cut(s) 561
Bst6I CTCTTC 1 cut(s) 537
BstACI GRCGYC 1 cut(s) 97
BstAUI TGTACA 1 cut(s) 388
BstC8I GCNNGC 1 cut(s) 592
BstDEI CTNAG 3 cut(s) 773, 831, 937
BstDSI CCRYGG 2 cut(s) 107, 684
BstF5I GGATG 2 cut(s) 1081, 1134
BstFNI CGCG 1 cut(s) 686
BstKTI GATC 4 cut(s) 87, 207, 403, 556
BstMAI GTCTC 1 cut(s) 1082
BstMBI GATC 4 cut(s) 84, 204, 400, 553
BstMWI GCNNNNNNNGC 1 cut(s) 683
BstNI CCWGG 1 cut(s) 144
BstNSI RCATGY 1 cut(s) 1066
BstSCI CCNGG 1 cut(s) 142
BstUI CGCG 1 cut(s) 686
BstV1I GCAGC 2 cut(s) 606, 746
BstV2I GAAGAC 2 cut(s) 554, 785
BstX2I RGATCY 1 cut(s) 400
BstXI CCANNNNNNTGG 1 cut(s) 862
BstYI RGATCY 1 cut(s) 400
Bsu15I ATCGAT 2 cut(s) 8, 1024
BsuRI GGCC 4 cut(s) 106, 112, 135, 147
BsuTUI ATCGAT 2 cut(s) 8, 1024
BtgI CCRYGG 2 cut(s) 107, 684
BtsCI GGATG 2 cut(s) 1081, 1134
BtsI GCAGTG 1 cut(s) 189
BtsIMutI CAGTG 1 cut(s) 189
Cac8I GCNNGC 1 cut(s) 592
CaiI CAGNNNCTG 1 cut(s) 125
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 5 cut(s) 129, 134, 146, 865, 1010
Cfr42I CCGCGG 1 cut(s) 687
ClaI ATCGAT 2 cut(s) 8, 1024
Csp6I GTAC 3 cut(s) 365, 389, 764
CviAII CATG 3 cut(s) 108, 626, 1063
CviQI GTAC 3 cut(s) 365, 389, 764
DdeI CTNAG 3 cut(s) 773, 831, 937
DpnI GATC 4 cut(s) 86, 206, 402, 555
DpnII GATC 4 cut(s) 84, 204, 400, 553
DraI TTTAAA 1 cut(s) 1146
EaeI YGGCCR 2 cut(s) 104, 110
Eam1104I CTCTTC 1 cut(s) 537
EarI CTCTTC 1 cut(s) 537
Eco130I CCWWGG 2 cut(s) 107, 567
Eco32I GATATC 1 cut(s) 652
Eco47I GGWCC 3 cut(s) 129, 865, 1010
Eco57I CTGAAG 1 cut(s) 411
EcoRII CCWGG 1 cut(s) 142
EcoRV GATATC 1 cut(s) 652
EcoT14I CCWWGG 2 cut(s) 107, 567
ErhI CCWWGG 2 cut(s) 107, 567
FaeI CATG 3 cut(s) 111, 629, 1066
FalI AAGNNNNNCTT 2 cut(s) 777, 809
FatI CATG 3 cut(s) 107, 625, 1062
FblI GTMKAC 1 cut(s) 100
Fnu4HI GCNGC 3 cut(s) 595, 687, 760
FokI GGATG 2 cut(s) 1068, 1121
Fsp4HI GCNGC 3 cut(s) 595, 687, 760
FspBI CTAG 2 cut(s) 395, 740
GluI GCNGC 3 cut(s) 595, 687, 760
HaeIII GGCC 4 cut(s) 106, 112, 135, 147
HapII CCGG 2 cut(s) 132, 179
Hin1I GRCGYC 1 cut(s) 97
Hin1II CATG 3 cut(s) 111, 629, 1066
HinfI GANTC 6 cut(s) 122, 200, 303, 454, 1026, 1095
HpaII CCGG 2 cut(s) 132, 179
HphI GGTGA 3 cut(s) 83, 132, 150
Hpy166II GTNNAC 2 cut(s) 101, 1013
Hpy188I TCNGA 3 cut(s) 287, 328, 340
Hpy188III TCNNGA 3 cut(s) 61, 468, 812
Hpy8I GTNNAC 2 cut(s) 101, 1013
Hpy99I CGWCG 1 cut(s) 99
HpyCH4III ACNGT 1 cut(s) 561
HpyCH4IV ACGT 2 cut(s) 97, 843
HpyCH4V TGCA 3 cut(s) 293, 703, 1020
HpyF10VI GCNNNNNNNGC 1 cut(s) 683
HpyF3I CTNAG 3 cut(s) 773, 831, 937
HpySE526I ACGT 2 cut(s) 97, 843
Hsp92I GRCGYC 1 cut(s) 97
Hsp92II CATG 3 cut(s) 111, 629, 1066
KspI CCGCGG 1 cut(s) 687
Kzo9I GATC 4 cut(s) 84, 204, 400, 553
LmnI GCTCC 3 cut(s) 487, 719, 1049
Lsp1109I GCAGC 2 cut(s) 606, 746
MaeI CTAG 2 cut(s) 395, 740
MaeII ACGT 2 cut(s) 97, 843
MaeIII GTNAC 2 cut(s) 61, 929
MalI GATC 4 cut(s) 86, 206, 402, 555
MboI GATC 4 cut(s) 84, 204, 400, 553
MboII GAAGA 7 cut(s) 49, 395, 410, 554, 559, 785, 977
MfeI CAATTG 4 cut(s) 360, 491, 635, 869
MflI RGATCY 1 cut(s) 400
MhlI GDGCHC 3 cut(s) 634, 811, 1046
MlsI TGGCCA 1 cut(s) 112
MluNI TGGCCA 1 cut(s) 112
MlyI GAGTC 1 cut(s) 448
MmeI TCCRAC 3 cut(s) 465, 529, 879
MnlI CCTC 3 cut(s) 522, 782, 971
Mox20I TGGCCA 1 cut(s) 112
MroXI GAANNNNTTC 1 cut(s) 402
MscI TGGCCA 1 cut(s) 112
MseI TTAA 3 cut(s) 14, 1109, 1145
MslI CAYNNNNRTG 1 cut(s) 1039
Msp20I TGGCCA 1 cut(s) 112
MspA1I CMGCKG 1 cut(s) 686
MspI CCGG 2 cut(s) 132, 179
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 4 cut(s) 360, 491, 635, 869
MvaI CCWGG 1 cut(s) 144
MvnI CGCG 1 cut(s) 686
MwoI GCNNNNNNNGC 1 cut(s) 683
NcoI CCATGG 1 cut(s) 107
NdeII GATC 4 cut(s) 84, 204, 400, 553
NlaIII CATG 3 cut(s) 111, 629, 1066
NlaIV GGNNCC 1 cut(s) 130
NmuCI GTSAC 2 cut(s) 61, 929
NspI RCATGY 1 cut(s) 1066
PciI ACATGT 1 cut(s) 1062
PcsI WCGNNNNNNNCGW 1 cut(s) 318
PdmI GAANNNNTTC 1 cut(s) 402
PfeI GAWTC 5 cut(s) 122, 200, 303, 1026, 1095
PflMI CCANNNNNTGG 3 cut(s) 125, 143, 755
PkrI GCNGC 3 cut(s) 596, 688, 761
PleI GAGTC 1 cut(s) 448
PpsI GAGTC 1 cut(s) 448
PscI ACATGT 1 cut(s) 1062
PshBI ATTAAT 1 cut(s) 14
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 130
PspPI GGNCC 5 cut(s) 129, 134, 146, 865, 1010
PstNI CAGNNNCTG 1 cut(s) 125
PsuI RGATCY 1 cut(s) 400
RsaI GTAC 3 cut(s) 366, 390, 765
RsaNI GTAC 3 cut(s) 365, 389, 764
RseI CAYNNNNRTG 1 cut(s) 1039
SacII CCGCGG 1 cut(s) 687
SaqAI TTAA 3 cut(s) 14, 1109, 1145
SatI GCNGC 3 cut(s) 595, 687, 760
Sau3AI GATC 4 cut(s) 84, 204, 400, 553
Sau96I GGNCC 5 cut(s) 129, 134, 146, 865, 1010
SchI GAGTC 1 cut(s) 448
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 3 cut(s) 634, 811, 1046
Sfr303I CCGCGG 1 cut(s) 687
SgrBI CCGCGG 1 cut(s) 687
SinI GGWCC 3 cut(s) 129, 865, 1010
SmiI ATTTAAAT 1 cut(s) 1146
SmiMI CAYNNNNRTG 1 cut(s) 1039
SmlI CTYRAG 1 cut(s) 810
SmoI CTYRAG 1 cut(s) 810
SsiI CCGC 3 cut(s) 116, 684, 686
SspI AATATT 1 cut(s) 344
SspMI CTAG 2 cut(s) 395, 740
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 2 cut(s) 107, 567
SwaI ATTTAAAT 1 cut(s) 1146
TaaI ACNGT 1 cut(s) 561
TaiI ACGT 2 cut(s) 100, 846
TaqI TCGA 3 cut(s) 8, 203, 1024
TatI WGTACW 1 cut(s) 388
TauI GCSGC 1 cut(s) 689
TfiI GAWTC 5 cut(s) 122, 200, 303, 1026, 1095
Tru1I TTAA 3 cut(s) 14, 1109, 1145
Tru9I TTAA 3 cut(s) 14, 1109, 1145
TscAI CASTG 1 cut(s) 189
TseFI GTSAC 2 cut(s) 61, 929
TseI GCWGC 2 cut(s) 594, 759
Tsp45I GTSAC 2 cut(s) 61, 929
TspDTI ATGAA 2 cut(s) 17, 260
TspGWI ACGGA 3 cut(s) 327, 566, 568
TspRI CASTG 1 cut(s) 189
Van91I CCANNNNNTGG 3 cut(s) 125, 143, 755
VpaK11BI GGWCC 3 cut(s) 129, 865, 1010
VspI ATTAAT 1 cut(s) 14
XapI RAATTY 3 cut(s) 679, 889, 943
XceI RCATGY 1 cut(s) 1066
XmiI GTMKAC 1 cut(s) 100
XmnI GAANNNNTTC 1 cut(s) 402
XspI CTAG 2 cut(s) 395, 740
ZraI GACGTC 1 cut(s) 98
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.