MD02G1126900.v1.1

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000393
Physical Location & Seq
Forward (+)
10261526 .. 10263477
1952 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1126900.v1.1.491

Sequence Viewer

Length: 1101 bp
ATGAACTTTCTCTCCCAAAAACTTCTTCCTTCATCTAAGTCTTCTCTCAAATTTCTTTCAGTCGTTATAGTTTTGTTCTGCTACTGCCATGCTGCTGCAGTGAAACTACCGAACAATGAAAAAATTCCGGCACTGATTGTTTTCGGAGATTCAATAGTGGATCCCGGAAACAACAACAATATTGGCACTATAGTCAAATGCAACTTCCCACCTTATGGGAGGGACTTCATAGGAAAACGGCCTACTGGAAGGTTTAGCAATGGCAGAGTTCCCTCAGACTTGATAGCTGAATCAGTTGGAGTGAAAAATATTTTACCAGCTTATTTGGATCCAAATCTGAAGATTGAAGACCTACTTACTGGTGTAAGTTTTGCCTCGGGTGGTTCAGGATATGATCCTCTCACTCCCCAAATCGTGTCTGTCCTATCATTATCAGATCAACTAGACCTGTTCAAAAATTACATACGCAAGATAACTGCAGCAGCGGGGGAGGAGAGGGCGGCAACTATAGTATCAAAAAGCATATACATTGTGTGCATAGGAAGCGACGACATTGCAAATACTTATTTATCTACACCAGTGAGGAGACCTTACTATGACATTCCAGCGTATACCGATCTCATGATTAACTCGGCTTCAAGTTTCTTACAGGAACTTTATGGACTCGGAGCAAGAAGAATTGGAGTAATAAATCTGCCGGCAATCGGGTGTGTGCCGTCACAGAGAACAGTGGGTGGAGGCATAAATAGGGATTGCTCCGAGACTGCTAACCAAGCGGCCCGCCTCTTCAACTCCAAGCTCTCGGCCAACATAGATGCCTTCAATAAGAGGCTTCCAGAAGCGAGGGTTGTCTACCTTGATATCTACTACACATTGCTTTCCCTCATCCAAAACCCTACTCAATATGGATTTGAAGTGGCAAATAAGGGCTGTTGTGGAACAGGAAACATCGAGGTTAGCGTCCTGTGTACTCGTTACTCTCCGGGAACCTGCAATGATTCATCGAAATACATATTCTGGGACAGCTATCATCCCACGGAGAAGGCATATGAGACCCTTGTCCCTCTGGTTTTCGATACGCAAATTCGTAAATTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

367

Amino Acids

40.09

Weight (kDa)

8.42

Isoelectric Point (pI)

37.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 46 - 353 3e-34 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 998
AccB7I CCANNNNNTGG 1 cut(s) 215
AccI GTMKAC 2 cut(s) 611, 852
AciI CCGC 4 cut(s) 485, 500, 776, 781
AclWI GGATC 5 cut(s) 155, 168, 323, 336, 389
AcoI YGGCCR 1 cut(s) 804
AcsI RAATTY 4 cut(s) 50, 123, 1083, 1091
AcuI CTGAAG 1 cut(s) 359
AfaI GTAC 1 cut(s) 970
AfiI CCNNNNNNNGG 2 cut(s) 215, 704
AgsI TTSAA 7 cut(s) 153, 347, 454, 639, 790, 823, 914
AjuI GAANNNNNNNTTGG 2 cut(s) 9, 41
AloI GAACNNNNNNTCC 1 cut(s) 28
AluBI AGCT 4 cut(s) 287, 320, 799, 1026
AluI AGCT 4 cut(s) 287, 320, 799, 1026
Alw26I GTCTC 3 cut(s) 580, 755, 1046
AlwI GGATC 5 cut(s) 155, 168, 323, 336, 389
Ama87I CYCGRG 1 cut(s) 376
AoxI GGCC 3 cut(s) 239, 777, 804
ApeKI GCWGC 4 cut(s) 92, 95, 479, 482
ApoI RAATTY 4 cut(s) 50, 123, 1083, 1091
Asp700I GAANNNNTTC 1 cut(s) 123
AspS9I GGNCC 1 cut(s) 778
AsuC2I CCSGG 2 cut(s) 165, 984
AvaI CYCGRG 1 cut(s) 376
BaeI ACNNNNGTAYC 2 cut(s) 495, 528
BamHI GGATCC 2 cut(s) 160, 328
BbsI GAAGAC 2 cut(s) 33, 354
BbvI GCAGC 4 cut(s) 79, 82, 491, 494
BceAI ACGGC 2 cut(s) 254, 700
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BcnI CCSGG 2 cut(s) 165, 984
BcoDI GTCTC 3 cut(s) 580, 755, 1046
BfaI CTAG 1 cut(s) 443
BfmI CTRYAG 4 cut(s) 96, 189, 477, 507
BfuAI ACCTGC 1 cut(s) 998
BisI GCNGC 6 cut(s) 93, 96, 480, 483, 501, 777
BlsI GCNGC 6 cut(s) 94, 97, 481, 484, 502, 778
Bme1390I CCNGG 2 cut(s) 165, 984
BmeT110I CYCGRG 1 cut(s) 376
BmgT120I GGNCC 1 cut(s) 778
BmiI GGNNCC 3 cut(s) 162, 330, 988
BmrFI CCNGG 2 cut(s) 165, 984
BmsI GCATC 1 cut(s) 805
BoxI GACNNNNGTC 1 cut(s) 1058
BpiI GAAGAC 2 cut(s) 33, 354
BpuMI CCSGG 2 cut(s) 165, 984
BsaBI GATNNNNATC 1 cut(s) 333
BsaI GGTCTC 2 cut(s) 580, 1046
BsaJI CCNNGG 2 cut(s) 375, 1035
BsaXI ACNNNNNCTCC 1 cut(s) 26
Bsc4I CCNNNNNNNGG 2 cut(s) 215, 704
Bse118I RCCGGY 1 cut(s) 697
Bse1I ACTGG 3 cut(s) 250, 364, 578
Bse3DI GCAATG 4 cut(s) 265, 552, 872, 1000
Bse8I GATNNNNATC 1 cut(s) 333
BseDI CCNNGG 2 cut(s) 375, 1035
BseGI GGATG 2 cut(s) 885, 1030
BseJI GATNNNNATC 1 cut(s) 333
BseLI CCNNNNNNNGG 2 cut(s) 215, 704
BseMI GCAATG 4 cut(s) 265, 552, 872, 1000
BseMII CTCAG 1 cut(s) 288
BseNI ACTGG 3 cut(s) 250, 364, 578
BseRI GAGGAG 2 cut(s) 506, 598
BseXI GCAGC 4 cut(s) 79, 82, 491, 494
BshFI GGCC 3 cut(s) 241, 779, 806
BsiHKCI CYCGRG 1 cut(s) 376
BsiSI CCGG 4 cut(s) 128, 165, 698, 983
BslFI GGGAC 3 cut(s) 236, 1034, 1046
BslI CCNNNNNNNGG 2 cut(s) 215, 704
BsmAI GTCTC 3 cut(s) 580, 755, 1046
BsmFI GGGAC 3 cut(s) 236, 1034, 1046
BsnI GGCC 3 cut(s) 241, 779, 806
Bso31I GGTCTC 2 cut(s) 580, 1046
BsoBI CYCGRG 1 cut(s) 376
Bsp143I GATC 5 cut(s) 160, 328, 394, 436, 616
BspACI CCGC 4 cut(s) 485, 500, 776, 781
BspANI GGCC 3 cut(s) 241, 779, 806
BspCNI CTCAG 1 cut(s) 287
BspHI TCATGA 1 cut(s) 621
BspLI GGNNCC 3 cut(s) 162, 330, 988
BspMAI CTGCAG 2 cut(s) 100, 481
BspMI ACCTGC 1 cut(s) 998
BspPI GGATC 5 cut(s) 155, 168, 323, 336, 389
BspTNI GGTCTC 2 cut(s) 580, 1046
BsrDI GCAATG 4 cut(s) 265, 552, 872, 1000
BsrFI RCCGGY 1 cut(s) 697
BsrI ACTGG 3 cut(s) 250, 364, 578
BssAI RCCGGY 1 cut(s) 697
BssECI CCNNGG 2 cut(s) 375, 1035
BssMI GATC 5 cut(s) 160, 328, 394, 436, 616
BssNAI GTATAC 1 cut(s) 612
Bst1107I GTATAC 1 cut(s) 612
Bst4CI ACNGT 1 cut(s) 730
Bst6I CTCTTC 1 cut(s) 791
BstC8I GCNNGC 2 cut(s) 699, 781
BstDEI CTNAG 2 cut(s) 36, 274
BstDSI CCRYGG 1 cut(s) 1035
BstF5I GGATG 2 cut(s) 885, 1030
BstKTI GATC 5 cut(s) 163, 331, 397, 439, 619
BstMAI GTCTC 3 cut(s) 580, 755, 1046
BstMBI GATC 5 cut(s) 160, 328, 394, 436, 616
BstMWI GCNNNNNNNGC 2 cut(s) 543, 773
BstPAI GACNNNNGTC 1 cut(s) 1058
BstSCI CCNGG 2 cut(s) 163, 982
BstSFI CTRYAG 4 cut(s) 96, 189, 477, 507
BstV1I GCAGC 4 cut(s) 79, 82, 491, 494
BstV2I GAAGAC 2 cut(s) 33, 354
BstX2I RGATCY 2 cut(s) 160, 328
BstYI RGATCY 2 cut(s) 160, 328
BstZ17I GTATAC 1 cut(s) 612
BsuRI GGCC 3 cut(s) 241, 779, 806
BtgI CCRYGG 1 cut(s) 1035
BtsCI GGATG 2 cut(s) 885, 1030
BtsI GCAGTG 1 cut(s) 105
BtsIMutI CAGTG 4 cut(s) 105, 131, 585, 735
BveI ACCTGC 1 cut(s) 998
Cac8I GCNNGC 2 cut(s) 699, 781
CciI TCATGA 1 cut(s) 621
Cfr10I RCCGGY 1 cut(s) 697
Cfr13I GGNCC 1 cut(s) 778
CseI GACGC 1 cut(s) 949
Csp6I GTAC 1 cut(s) 969
CviAII CATG 2 cut(s) 89, 622
CviQI GTAC 1 cut(s) 969
DdeI CTNAG 2 cut(s) 36, 274
DpnI GATC 5 cut(s) 162, 330, 396, 438, 618
DpnII GATC 5 cut(s) 160, 328, 394, 436, 616
EaeI YGGCCR 1 cut(s) 804
Eam1104I CTCTTC 1 cut(s) 791
EarI CTCTTC 1 cut(s) 791
Eco31I GGTCTC 2 cut(s) 580, 1046
Eco32I GATATC 1 cut(s) 862
Eco57I CTGAAG 1 cut(s) 359
Eco88I CYCGRG 1 cut(s) 376
EcoRV GATATC 1 cut(s) 862
FaeI CATG 2 cut(s) 92, 625
FalI AAGNNNNNCTT 2 cut(s) 339, 371
FaqI GGGAC 3 cut(s) 236, 1034, 1046
FatI CATG 2 cut(s) 88, 621
FauI CCCGC 2 cut(s) 478, 788
FauNDI CATATG 1 cut(s) 1048
FblI GTMKAC 2 cut(s) 611, 852
Fnu4HI GCNGC 6 cut(s) 93, 96, 480, 483, 501, 777
FokI GGATG 2 cut(s) 872, 1017
Fsp4HI GCNGC 6 cut(s) 93, 96, 480, 483, 501, 777
FspBI CTAG 1 cut(s) 443
GluI GCNGC 6 cut(s) 93, 96, 480, 483, 501, 777
HaeIII GGCC 3 cut(s) 241, 779, 806
HapII CCGG 4 cut(s) 128, 165, 698, 983
HgaI GACGC 1 cut(s) 949
Hin1II CATG 2 cut(s) 92, 625
HinfI GANTC 4 cut(s) 149, 290, 663, 998
HpaII CCGG 4 cut(s) 128, 165, 698, 983
Hpy166II GTNNAC 3 cut(s) 612, 853, 969
Hpy188I TCNGA 7 cut(s) 146, 277, 339, 436, 668, 760, 1100
Hpy188III TCNNGA 3 cut(s) 387, 622, 836
Hpy8I GTNNAC 3 cut(s) 612, 853, 969
Hpy99I CGWCG 1 cut(s) 551
HpyAV CCTTC 4 cut(s) 39, 243, 829, 1036
HpyCH4III ACNGT 1 cut(s) 730
HpyCH4V TGCA 6 cut(s) 98, 201, 479, 537, 557, 993
HpyF10VI GCNNNNNNNGC 2 cut(s) 543, 773
HpyF3I CTNAG 2 cut(s) 36, 274
Hsp92II CATG 2 cut(s) 92, 625
KroI GCCGGC 1 cut(s) 697
KroNI GCCGGC 1 cut(s) 699
Kzo9I GATC 5 cut(s) 160, 328, 394, 436, 616
LmnI GCTCC 2 cut(s) 668, 761
Lsp1109I GCAGC 4 cut(s) 79, 82, 491, 494
LweI GCATC 1 cut(s) 805
MaeI CTAG 1 cut(s) 443
MaeIII GTNAC 2 cut(s) 717, 974
MalI GATC 5 cut(s) 162, 330, 396, 438, 618
MboI GATC 5 cut(s) 160, 328, 394, 436, 616
MboII GAAGA 7 cut(s) 17, 33, 352, 359, 687, 778, 1087
MflI RGATCY 2 cut(s) 160, 328
MluCI AATT 6 cut(s) 50, 123, 457, 678, 1083, 1091
MlyI GAGTC 1 cut(s) 657
MmeI TCCRAC 1 cut(s) 277
MroNI GCCGGC 1 cut(s) 697
MroXI GAANNNNTTC 1 cut(s) 123
MseI TTAA 1 cut(s) 627
MspA1I CMGCKG 1 cut(s) 485
MspI CCGG 4 cut(s) 128, 165, 698, 983
MspR9I CCNGG 2 cut(s) 165, 984
MwoI GCNNNNNNNGC 2 cut(s) 543, 773
NaeI GCCGGC 1 cut(s) 699
NciI CCSGG 2 cut(s) 165, 984
NdeI CATATG 1 cut(s) 1048
NdeII GATC 5 cut(s) 160, 328, 394, 436, 616
NgoMIV GCCGGC 1 cut(s) 697
NlaIII CATG 2 cut(s) 92, 625
NlaIV GGNNCC 3 cut(s) 162, 330, 988
NmeAIII GCCGAG 2 cut(s) 611, 782
NmuCI GTSAC 1 cut(s) 717
PagI TCATGA 1 cut(s) 621
PcsI WCGNNNNNNNCGW 1 cut(s) 957
PdiI GCCGGC 1 cut(s) 699
PdmI GAANNNNTTC 1 cut(s) 123
PfeI GAWTC 3 cut(s) 149, 290, 998
PflMI CCANNNNNTGG 1 cut(s) 215
PfoI TCCNGGA 2 cut(s) 163, 982
PkrI GCNGC 6 cut(s) 94, 97, 481, 484, 502, 778
PleI GAGTC 1 cut(s) 657
PpsI GAGTC 1 cut(s) 657
PshAI GACNNNNGTC 1 cut(s) 1058
PspN4I GGNNCC 3 cut(s) 162, 330, 988
PspPI GGNCC 1 cut(s) 778
PstI CTGCAG 2 cut(s) 100, 481
PsuI RGATCY 2 cut(s) 160, 328
RsaI GTAC 1 cut(s) 970
RsaNI GTAC 1 cut(s) 969
SaqAI TTAA 1 cut(s) 627
SatI GCNGC 6 cut(s) 93, 96, 480, 483, 501, 777
Sau3AI GATC 5 cut(s) 160, 328, 394, 436, 616
Sau96I GGNCC 1 cut(s) 778
SchI GAGTC 1 cut(s) 657
ScrFI CCNGG 2 cut(s) 165, 984
SfaNI GCATC 1 cut(s) 805
SfcI CTRYAG 4 cut(s) 96, 189, 477, 507
Sse9I AATT 6 cut(s) 50, 123, 457, 678, 1083, 1091
SsiI CCGC 4 cut(s) 485, 500, 776, 781
SspI AATATT 2 cut(s) 181, 310
SspMI CTAG 1 cut(s) 443
StyD4I CCNGG 2 cut(s) 163, 982
TaaI ACNGT 1 cut(s) 730
TaqI TCGA 3 cut(s) 951, 1004, 1074
TasI AATT 6 cut(s) 50, 123, 457, 678, 1083, 1091
TatI WGTACW 1 cut(s) 968
TauI GCSGC 2 cut(s) 503, 779
TfiI GAWTC 3 cut(s) 149, 290, 998
Tru1I TTAA 1 cut(s) 627
Tru9I TTAA 1 cut(s) 627
TscAI CASTG 4 cut(s) 105, 138, 585, 735
TseFI GTSAC 1 cut(s) 717
TseI GCWGC 4 cut(s) 92, 95, 479, 482
Tsp45I GTSAC 1 cut(s) 717
TspDTI ATGAA 5 cut(s) 17, 21, 132, 217, 990
TspGWI ACGGA 1 cut(s) 1052
TspRI CASTG 4 cut(s) 105, 138, 585, 735
Van91I CCANNNNNTGG 1 cut(s) 215
XapI RAATTY 4 cut(s) 50, 123, 1083, 1091
XmiI GTMKAC 2 cut(s) 611, 852
XmnI GAANNNNTTC 1 cut(s) 123
XspI CTAG 1 cut(s) 443
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.