FvH4_2g38111

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb2
OG0000393
Physical Location & Seq
Reverse (-)
27609278 .. 27611191
1914 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_2g38111.t2

Sequence Viewer

Length: 1071 bp
ATGAAATCCAACTTTCTATGGTTGCTTCCTTCTCTGGATCTGATATTTACTTGTGCCCTAGCTATTGTTAAGCTACCGGAAAATGTAACAGTTCCGGCTGTTTTTATGTTCGGAGATTCGATAGTCGACACAGGCAACAATAACAACCTAACAAGGACCTTTCTGAAGAGCAACTTCCCTCCCTATGGAAGAGACTTTCTTGGAGGGGTTCCAACCGGAAGATTTTGCAACGGCAAGGTTCCATCGGACTTTGTCGTGGAGGAACTGGGGATCAAAGAGTACTTACCAGCCTATCTTGATCCAGATCTTCAAGAGACGGACTTGCTTACTGGAGTAAACTTTGCTTTCAGCGGCTCGGGGTTTGATCCCTTGACATCTATAGTTATGCAAGTGATGCCATTATCAGAACAGTTAGACATGTTTAAGGAATGCATTGAGAGGCTGAACAAGTTCGTCGGAGATGAGAAGGCTAACAACATTATAAGCAACAGCCTTTTCATGTTGGAATCAGGAAGTGATGACCTCGTGAATACCTACTATCATACACCTACACGTTTCTTTCAGTACGATATTCATGCCTACACTGATCTCCTTCTCAGGGAGGCTTCATCATTTGTGCAGAAATTATATGAATTGGGGGCACGGAGGATAGGGATCATAGGTCTGCCACCAGTTGGATGTGTGCCATCACAGAGAACATTAGCAGGAGGTAAAGAAAGACGGTGCGCCGACAAGTACAACAAAGCGGCTGAGCTACTCAACACCAAGCTTTCAGCTATGGCGGATTTCCAGACCAGTAACCTAACAGATGCCAGAGTCGTCTACATGGATGCATACTATCCTTTCCTTGATATCATTCAACACATACACAAATATGGATTTGAAGTGGTGAATAAAGGGTGTTGTGGAACAGGGATGTTAGAGGTGACCATTCTTTGTAATAAATGGTCACCGAAAACATGTTCAAACGCGTCGACATATGTGTTTTGGGACAGTTATCATCCTACGGAAAGAGCATATCAGATCCTTAGCCATCAAGTACTTCAAAACACAATTCATTCTTTCTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

357

Amino Acids

40.07

Weight (kDa)

5.42

Isoelectric Point (pI)

37.04

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 293 2.1e-22 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 482
AccB7I CCANNNNNTGG 1 cut(s) 674
AccI GTMKAC 3 cut(s) 126, 822, 974
AccII CGCG 1 cut(s) 971
AciI CCGC 3 cut(s) 351, 746, 782
AclWI GGATC 6 cut(s) 45, 278, 293, 359, 662, 1018
AcuI CTGAAG 1 cut(s) 185
AfaI GTAC 4 cut(s) 281, 566, 737, 1041
AfiI CCNNNNNNNGG 3 cut(s) 185, 598, 674
AflIII ACRYGT 4 cut(s) 417, 551, 959, 969
AgsI TTSAA 5 cut(s) 311, 860, 884, 966, 1046
AluBI AGCT 5 cut(s) 62, 73, 754, 769, 776
AluI AGCT 5 cut(s) 62, 73, 754, 769, 776
Alw26I GTCTC 2 cut(s) 186, 308
AlwI GGATC 6 cut(s) 45, 278, 293, 359, 662, 1018
Ama87I CYCGRG 1 cut(s) 355
Asp700I GAANNNNTTC 1 cut(s) 449
AspLEI GCGC 1 cut(s) 728
AspS9I GGNCC 1 cut(s) 156
AsuHPI GGTGA 3 cut(s) 901, 937, 942
AvaI CYCGRG 1 cut(s) 355
AvaII GGWCC 1 cut(s) 156
BaeGI GKGCMC 2 cut(s) 58, 643
BauI CACGAG 1 cut(s) 524
BccI CCATC 3 cut(s) 250, 694, 1041
BceAI ACGGC 1 cut(s) 247
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BcoDI GTCTC 2 cut(s) 186, 308
BfaI CTAG 1 cut(s) 59
BfmI CTRYAG 1 cut(s) 378
BglII AGATCT 1 cut(s) 304
BisI GCNGC 2 cut(s) 352, 747
BlpI GCTNAGC 1 cut(s) 750
BlsI GCNGC 2 cut(s) 353, 748
BmcAI AGTACT 2 cut(s) 281, 1041
Bme18I GGWCC 1 cut(s) 156
BmeT110I CYCGRG 1 cut(s) 355
BmgT120I GGNCC 1 cut(s) 156
BmiI GGNNCC 2 cut(s) 210, 240
BmrI ACTGGG 1 cut(s) 275
BmsI GCATC 3 cut(s) 384, 799, 820
BmuI ACTGGG 1 cut(s) 275
BpmI CTGGAG 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 1028
Bpu1102I GCTNAGC 1 cut(s) 750
BsaBI GATNNNNATC 2 cut(s) 303, 653
BsaWI WCCGGW 2 cut(s) 76, 215
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 598, 674
Bse1I ACTGG 4 cut(s) 270, 334, 671, 795
Bse8I GATNNNNATC 2 cut(s) 303, 653
BseGI GGATG 4 cut(s) 683, 835, 921, 1000
BseJI GATNNNNATC 2 cut(s) 303, 653
BseLI CCNNNNNNNGG 3 cut(s) 185, 598, 674
BseMII CTCAG 2 cut(s) 610, 741
BseNI ACTGG 4 cut(s) 270, 334, 671, 795
BseSI GKGCMC 2 cut(s) 58, 643
BsgI GTGCAG 1 cut(s) 638
Bsh1236I CGCG 1 cut(s) 971
BsiHKCI CYCGRG 1 cut(s) 355
BsiSI CCGG 3 cut(s) 77, 95, 216
BslFI GGGAC 1 cut(s) 1004
BslI CCNNNNNNNGG 3 cut(s) 185, 598, 674
BsmAI GTCTC 2 cut(s) 186, 308
BsmBI CGTCTC 1 cut(s) 308
BsmFI GGGAC 1 cut(s) 1004
BsmI GAATGC 1 cut(s) 434
BsoBI CYCGRG 1 cut(s) 355
Bsp1286I GDGCHC 2 cut(s) 58, 643
Bsp143I GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
Bsp1720I GCTNAGC 1 cut(s) 750
BspACI CCGC 3 cut(s) 351, 746, 782
BspCNI CTCAG 2 cut(s) 609, 742
BspFNI CGCG 1 cut(s) 971
BspLI GGNNCC 2 cut(s) 210, 240
BspPI GGATC 6 cut(s) 45, 278, 293, 359, 662, 1018
BspQI GCTCTTC 1 cut(s) 161
BsrI ACTGG 4 cut(s) 270, 334, 671, 795
BssMI GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
BssSI CACGAG 1 cut(s) 524
Bst2BI CACGAG 1 cut(s) 524
Bst4CI ACNGT 4 cut(s) 91, 411, 723, 995
Bst6I CTCTTC 2 cut(s) 161, 184
BstAPI GCANNNNNTGC 1 cut(s) 394
BstDEI CTNAG 3 cut(s) 596, 750, 1028
BstEII GGTNACC 2 cut(s) 925, 948
BstF5I GGATG 4 cut(s) 683, 835, 921, 1000
BstFNI CGCG 1 cut(s) 971
BstHHI GCGC 1 cut(s) 728
BstKTI GATC 8 cut(s) 40, 273, 301, 307, 367, 589, 657, 1026
BstMAI GTCTC 2 cut(s) 186, 308
BstMBI GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
BstMWI GCNNNNNNNGC 1 cut(s) 394
BstNSI RCATGY 2 cut(s) 421, 963
BstPI GGTNACC 2 cut(s) 925, 948
BstSFI CTRYAG 1 cut(s) 378
BstSLI GKGCMC 2 cut(s) 58, 643
BstUI CGCG 1 cut(s) 971
BstX2I RGATCY 3 cut(s) 37, 304, 1023
BstYI RGATCY 3 cut(s) 37, 304, 1023
BtsCI GGATG 4 cut(s) 683, 835, 921, 1000
BtsIMutI CAGTG 1 cut(s) 582
CfoI GCGC 1 cut(s) 728
Cfr13I GGNCC 1 cut(s) 156
CseI GACGC 1 cut(s) 960
Csp6I GTAC 4 cut(s) 280, 565, 736, 1040
CviAII CATG 5 cut(s) 418, 499, 575, 826, 960
CviQI GTAC 4 cut(s) 280, 565, 736, 1040
DdeI CTNAG 3 cut(s) 596, 750, 1028
DpnI GATC 8 cut(s) 39, 272, 300, 306, 366, 588, 656, 1025
DpnII GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
Eam1104I CTCTTC 2 cut(s) 161, 184
EarI CTCTTC 2 cut(s) 161, 184
EciI GGCGGA 1 cut(s) 797
Eco32I GATATC 1 cut(s) 853
Eco47I GGWCC 1 cut(s) 156
Eco57I CTGAAG 1 cut(s) 185
Eco88I CYCGRG 1 cut(s) 355
Eco91I GGTNACC 2 cut(s) 925, 948
EcoO109I RGGNCCY 1 cut(s) 156
EcoO65I GGTNACC 2 cut(s) 925, 948
EcoRV GATATC 1 cut(s) 853
EcoT22I ATGCAT 2 cut(s) 434, 835
Esp3I CGTCTC 1 cut(s) 308
FaeI CATG 5 cut(s) 421, 502, 578, 829, 963
FalI AAGNNNNNCTT 2 cut(s) 158, 190
FaqI GGGAC 1 cut(s) 1004
FatI CATG 5 cut(s) 417, 498, 574, 825, 959
FauNDI CATATG 1 cut(s) 979
FblI GTMKAC 3 cut(s) 126, 822, 974
Fnu4HI GCNGC 2 cut(s) 352, 747
FokI GGATG 4 cut(s) 690, 842, 928, 987
Fsp4HI GCNGC 2 cut(s) 352, 747
FspBI CTAG 1 cut(s) 59
GlaI GCGC 1 cut(s) 727
GluI GCNGC 2 cut(s) 352, 747
GsuI CTGGAG 1 cut(s) 351
HapII CCGG 3 cut(s) 77, 95, 216
HgaI GACGC 1 cut(s) 960
HhaI GCGC 1 cut(s) 728
Hin1II CATG 5 cut(s) 421, 502, 578, 829, 963
Hin6I GCGC 1 cut(s) 726
HinP1I GCGC 1 cut(s) 726
HincII GTYRAC 2 cut(s) 127, 975
HindII GTYRAC 2 cut(s) 127, 975
HindIII AAGCTT 1 cut(s) 767
HinfI GANTC 3 cut(s) 116, 506, 816
HpaII CCGG 3 cut(s) 77, 95, 216
HphI GGTGA 3 cut(s) 901, 937, 942
Hpy166II GTNNAC 4 cut(s) 127, 337, 823, 975
Hpy188I TCNGA 7 cut(s) 42, 113, 165, 247, 406, 458, 1023
Hpy188III TCNNGA 7 cut(s) 35, 296, 302, 311, 510, 526, 790
Hpy8I GTNNAC 4 cut(s) 127, 337, 823, 975
Hpy99I CGWCG 2 cut(s) 458, 976
HpyAV CCTTC 3 cut(s) 39, 460, 602
HpyCH4III ACNGT 4 cut(s) 91, 411, 723, 995
HpyCH4IV ACGT 1 cut(s) 553
HpyCH4V TGCA 5 cut(s) 228, 388, 432, 619, 833
HpyF10VI GCNNNNNNNGC 1 cut(s) 394
HpyF3I CTNAG 3 cut(s) 596, 750, 1028
HpySE526I ACGT 1 cut(s) 553
Hsp92II CATG 5 cut(s) 421, 502, 578, 829, 963
HspAI GCGC 1 cut(s) 726
Kzo9I GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
LguI GCTCTTC 1 cut(s) 161
LweI GCATC 3 cut(s) 384, 799, 820
MaeI CTAG 1 cut(s) 59
MaeII ACGT 1 cut(s) 553
MaeIII GTNAC 4 cut(s) 85, 797, 925, 948
MalI GATC 8 cut(s) 39, 272, 300, 306, 366, 588, 656, 1025
MboI GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
MboII GAAGA 5 cut(s) 178, 201, 231, 299, 1057
MflI RGATCY 3 cut(s) 37, 304, 1023
MhlI GDGCHC 2 cut(s) 58, 643
MluCI AATT 3 cut(s) 623, 632, 1053
MluI ACGCGT 1 cut(s) 969
MlyI GAGTC 1 cut(s) 825
MmeI TCCRAC 5 cut(s) 33, 236, 436, 483, 655
MnlI CCTC 9 cut(s) 189, 197, 253, 432, 533, 595, 639, 701, 916
Mph1103I ATGCAT 2 cut(s) 434, 835
MroXI GAANNNNTTC 1 cut(s) 449
MseI TTAA 2 cut(s) 69, 423
MslI CAYNNNNRTG 1 cut(s) 873
MspA1I CMGCKG 1 cut(s) 351
MspI CCGG 3 cut(s) 77, 95, 216
Mva1269I GAATGC 1 cut(s) 434
MvnI CGCG 1 cut(s) 971
MwoI GCNNNNNNNGC 1 cut(s) 394
NdeI CATATG 1 cut(s) 979
NdeII GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
NlaIII CATG 5 cut(s) 421, 502, 578, 829, 963
NlaIV GGNNCC 2 cut(s) 210, 240
NmuCI GTSAC 2 cut(s) 925, 948
NsiI ATGCAT 2 cut(s) 434, 835
NspI RCATGY 2 cut(s) 421, 963
PciI ACATGT 2 cut(s) 417, 959
PciSI GCTCTTC 1 cut(s) 161
PctI GAATGC 1 cut(s) 434
PdmI GAANNNNTTC 1 cut(s) 449
PfeI GAWTC 2 cut(s) 116, 506
PflFI GACNNNGTC 1 cut(s) 251
PflMI CCANNNNNTGG 1 cut(s) 674
PkrI GCNGC 2 cut(s) 353, 748
PleI GAGTC 1 cut(s) 824
PpsI GAGTC 1 cut(s) 824
PpuMI RGGWCCY 1 cut(s) 156
PscI ACATGT 2 cut(s) 417, 959
PsiI TTATAA 1 cut(s) 482
Psp5II RGGWCCY 1 cut(s) 156
PspEI GGTNACC 2 cut(s) 925, 948
PspN4I GGNNCC 2 cut(s) 210, 240
PspPI GGNCC 1 cut(s) 156
PspPPI RGGWCCY 1 cut(s) 156
PsuI RGATCY 3 cut(s) 37, 304, 1023
PsyI GACNNNGTC 1 cut(s) 251
RsaI GTAC 4 cut(s) 281, 566, 737, 1041
RsaNI GTAC 4 cut(s) 280, 565, 736, 1040
RseI CAYNNNNRTG 1 cut(s) 873
SalI GTCGAC 2 cut(s) 125, 973
SapI GCTCTTC 1 cut(s) 161
SaqAI TTAA 2 cut(s) 69, 423
SatI GCNGC 2 cut(s) 352, 747
Sau3AI GATC 8 cut(s) 37, 270, 298, 304, 364, 586, 654, 1023
Sau96I GGNCC 1 cut(s) 156
ScaI AGTACT 2 cut(s) 281, 1041
SchI GAGTC 1 cut(s) 825
SduI GDGCHC 2 cut(s) 58, 643
SfaNI GCATC 3 cut(s) 384, 799, 820
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 1 cut(s) 156
SmiMI CAYNNNNRTG 1 cut(s) 873
Sse9I AATT 3 cut(s) 623, 632, 1053
SsiI CCGC 3 cut(s) 351, 746, 782
SspMI CTAG 1 cut(s) 59
TaaI ACNGT 4 cut(s) 91, 411, 723, 995
TaiI ACGT 1 cut(s) 556
TaqI TCGA 3 cut(s) 119, 126, 974
TasI AATT 3 cut(s) 623, 632, 1053
TatI WGTACW 3 cut(s) 279, 735, 1039
TauI GCSGC 2 cut(s) 354, 749
TfiI GAWTC 2 cut(s) 116, 506
Tru1I TTAA 2 cut(s) 69, 423
Tru9I TTAA 2 cut(s) 69, 423
TscAI CASTG 1 cut(s) 589
TseFI GTSAC 2 cut(s) 925, 948
Tsp45I GTSAC 2 cut(s) 925, 948
TspDTI ATGAA 6 cut(s) 17, 487, 563, 597, 645, 1046
TspGWI ACGGA 3 cut(s) 332, 658, 1022
TspRI CASTG 1 cut(s) 589
Tth111I GACNNNGTC 1 cut(s) 251
Van91I CCANNNNNTGG 1 cut(s) 674
VpaK11BI GGWCC 1 cut(s) 156
XceI RCATGY 2 cut(s) 421, 963
XmiI GTMKAC 3 cut(s) 126, 822, 974
XmnI GAANNNNTTC 1 cut(s) 449
XspI CTAG 1 cut(s) 59
ZrmI AGTACT 2 cut(s) 281, 1041
Zsp2I ATGCAT 2 cut(s) 434, 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.