Rw2G009510

GDSL esterase lipase EXL3-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0000393
Physical Location & Seq
Reverse (-)
9640962 .. 9642796
1835 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G009510.1

Sequence Viewer

Length: 951 bp
ATGGCTTTTCTCTCCCCAAAAATTCTTCCTTCAGCTTCATCTGTCAGAGTTTCTGTGATTATCTTATTTATATTGCTCTCCCATAATGTTGCTGCTATAACACTACCCAAAAATGGAACAACTCCAGCGGTGTTTGCTTTTGGAGATTCAATATTGGATACTGGCAATAACGATTACCTCATTTCTATGACCAAAAGCAATTTCCCACCTTATGGGAGAGACTTTATGGGAGGAATGCCTACAGGAAGGTTTAGCAATGGAAGAGTCCCCTCAGACTTCTCTGGCATTTCATTATCAGATCAATTAGACTTCTTCAAAGAATACAAAAGGAGGATAACTACAGCAGTTGGGGAGGAAAGAACAGCCACTATAATATCAAAAAGTGTATACGTTGTCTCCGTTGGAAGCAATGACATTGCAAATACTTATTTCTCTACACCACTGAGGAGCTCTCACTATGACATTCCAGCTTATACAGATCTCCTGCTCGAATCAGCAATAAATTTTGTTCAGGAACTTTATGCACTGGGAGCGAGAGTCATTGGAGTATTCAATTTTCCACCACTTGGGTGCTTGCCATCACAGAGAACACAGCATGGAGGCATAGTGAGGGCGTGCTACGAGGCCGAGAACCAAGCAGCAATCCTCTTCAACTCTAAGCTCTCCCCCCAAATAGATTCCCTCAATAAGAGACTTCCAGAAGCAGATGTCATTTACCTTGATATCTATACCCCTTTGCTTTCCATGATCCAAAACCCTGCTCAATATGGATTTGAAGTGGTGGATAAAGGATGCTGTGGAACAGGATATATTGAGGCTGGTCCTTTGTGTAACAAAGACTCCCCAGGTACTTGCAGTGACCCGTCAAAGTACCTATTCTGGGATGCTTACCATCCCACGGAGACAGGATACAGGTTCCTCACCTCTATGATTTCCAAACAATCATTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

316

Amino Acids

34.64

Weight (kDa)

5.83

Isoelectric Point (pI)

39.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 44 - 307 4.6e-28 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 949
AccB7I CCANNNNNTGG 2 cut(s) 212, 566
AccI GTMKAC 1 cut(s) 387
AciI CCGC 1 cut(s) 128
AclWI GGATC 1 cut(s) 742
AcsI RAATTY 2 cut(s) 21, 502
AcuI CTGAAG 1 cut(s) 15
AfaI GTAC 2 cut(s) 850, 872
AfiI CCNNNNNNNGG 5 cut(s) 113, 212, 566, 880, 898
AgsI TTSAA 5 cut(s) 150, 316, 553, 652, 776
AjnI CCWGG 1 cut(s) 844
AjuI GAANNNNNNNTTGG 2 cut(s) 185, 217
AleI CACNNNNGTG 1 cut(s) 568
AluBI AGCT 4 cut(s) 35, 450, 470, 661
AluI AGCT 4 cut(s) 35, 450, 470, 661
Alw21I GWGCWC 1 cut(s) 452
Alw26I GTCTC 4 cut(s) 213, 400, 685, 896
AlwI GGATC 1 cut(s) 742
AoxI GGCC 1 cut(s) 624
ApeKI GCWGC 2 cut(s) 92, 638
ApoI RAATTY 2 cut(s) 21, 502
AspS9I GGNCC 1 cut(s) 821
AsuHPI GGTGA 1 cut(s) 913
AvaII GGWCC 1 cut(s) 821
BanII GRGCYC 1 cut(s) 452
Bbv12I GWGCWC 1 cut(s) 452
BbvI GCAGC 2 cut(s) 79, 650
BccI CCATC 2 cut(s) 586, 900
BciT130I CCWGG 1 cut(s) 846
BciVI GTATCC 2 cut(s) 151, 902
BcoDI GTCTC 4 cut(s) 213, 400, 685, 896
BfmI CTRYAG 2 cut(s) 240, 339
BfuI GTATCC 2 cut(s) 151, 902
BglII AGATCT 1 cut(s) 478
BisI GCNGC 2 cut(s) 93, 639
BlsI GCNGC 2 cut(s) 94, 640
Bme1390I CCNGG 1 cut(s) 846
Bme18I GGWCC 1 cut(s) 821
BmgT120I GGNCC 1 cut(s) 821
BmiI GGNNCC 1 cut(s) 917
BmrFI CCNGG 1 cut(s) 846
BmrI ACTGGG 1 cut(s) 536
BmsI GCATC 2 cut(s) 782, 874
BmuI ACTGGG 1 cut(s) 536
BplI GAGNNNNNCTC 2 cut(s) 436, 468
BpmI CTGGAG 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 844, 897
BsaXI ACNNNNNCTCC 8 cut(s) 380, 410, 522, 552, 591, 621, 824, 854
Bsc4I CCNNNNNNNGG 5 cut(s) 113, 212, 566, 880, 898
Bse1I ACTGG 2 cut(s) 166, 531
Bse3DI GCAATG 3 cut(s) 262, 414, 415
BseBI CCWGG 1 cut(s) 846
BseDI CCNNGG 2 cut(s) 844, 897
BseGI GGATG 3 cut(s) 797, 889, 892
BseLI CCNNNNNNNGG 5 cut(s) 113, 212, 566, 880, 898
BseMI GCAATG 3 cut(s) 262, 414, 415
BseMII CTCAG 2 cut(s) 285, 434
BseNI ACTGG 2 cut(s) 166, 531
BseRI GAGGAG 1 cut(s) 460
BseXI GCAGC 2 cut(s) 79, 650
BshFI GGCC 1 cut(s) 626
BsiHKAI GWGCWC 1 cut(s) 452
BslFI GGGAC 1 cut(s) 251
BslI CCNNNNNNNGG 5 cut(s) 113, 212, 566, 880, 898
BsmAI GTCTC 4 cut(s) 213, 400, 685, 896
BsmFI GGGAC 1 cut(s) 251
BsmI GAATGC 1 cut(s) 240
BsnI GGCC 1 cut(s) 626
Bsp1286I GDGCHC 1 cut(s) 452
Bsp143I GATC 3 cut(s) 298, 478, 747
BspACI CCGC 1 cut(s) 128
BspANI GGCC 1 cut(s) 626
BspCNI CTCAG 2 cut(s) 284, 435
BspLI GGNNCC 1 cut(s) 917
BspPI GGATC 1 cut(s) 742
BsrDI GCAATG 3 cut(s) 262, 414, 415
BsrI ACTGG 2 cut(s) 166, 531
BssECI CCNNGG 2 cut(s) 844, 897
BssMI GATC 3 cut(s) 298, 478, 747
BssNAI GTATAC 1 cut(s) 388
Bst1107I GTATAC 1 cut(s) 388
Bst2UI CCWGG 1 cut(s) 846
Bst6I CTCTTC 2 cut(s) 256, 653
BstC8I GCNNGC 2 cut(s) 575, 616
BstDEI CTNAG 3 cut(s) 271, 443, 657
BstDSI CCRYGG 1 cut(s) 897
BstF5I GGATG 3 cut(s) 797, 889, 892
BstKTI GATC 3 cut(s) 301, 481, 750
BstMAI GTCTC 4 cut(s) 213, 400, 685, 896
BstMBI GATC 3 cut(s) 298, 478, 747
BstMWI GCNNNNNNNGC 2 cut(s) 134, 530
BstNI CCWGG 1 cut(s) 846
BstSCI CCNGG 1 cut(s) 844
BstSFI CTRYAG 2 cut(s) 240, 339
BstV1I GCAGC 2 cut(s) 79, 650
BstX2I RGATCY 1 cut(s) 478
BstYI RGATCY 1 cut(s) 478
BstZ17I GTATAC 1 cut(s) 388
BsuI GTATCC 2 cut(s) 151, 902
BsuRI GGCC 1 cut(s) 626
BtgI CCRYGG 1 cut(s) 897
BtsCI GGATG 3 cut(s) 797, 889, 892
BtsI GCAGTG 1 cut(s) 862
BtsIMutI CAGTG 3 cut(s) 440, 524, 862
Cac8I GCNNGC 2 cut(s) 575, 616
Cfr13I GGNCC 1 cut(s) 821
Csp6I GTAC 2 cut(s) 849, 871
CviAII CATG 2 cut(s) 596, 745
CviJI RGCY 8 cut(s) 5, 35, 365, 450, 470, 626, 661, 818
CviKI_1 RGCY 8 cut(s) 5, 35, 365, 450, 470, 626, 661, 818
CviQI GTAC 2 cut(s) 849, 871
DdeI CTNAG 3 cut(s) 271, 443, 657
DpnI GATC 3 cut(s) 300, 480, 749
DpnII GATC 3 cut(s) 298, 478, 747
Eam1104I CTCTTC 2 cut(s) 256, 653
EarI CTCTTC 2 cut(s) 256, 653
Ecl136II GAGCTC 1 cut(s) 450
Eco24I GRGCYC 1 cut(s) 452
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 1 cut(s) 821
Eco53kI GAGCTC 1 cut(s) 450
Eco57I CTGAAG 1 cut(s) 15
EcoICRI GAGCTC 1 cut(s) 450
EcoRII CCWGG 1 cut(s) 844
EcoRV GATATC 1 cut(s) 724
EcoT38I GRGCYC 1 cut(s) 452
FaeI CATG 2 cut(s) 599, 748
FaqI GGGAC 1 cut(s) 251
FatI CATG 2 cut(s) 595, 744
FblI GTMKAC 1 cut(s) 387
Fnu4HI GCNGC 2 cut(s) 93, 639
FokI GGATG 3 cut(s) 804, 879, 896
FriOI GRGCYC 1 cut(s) 452
Fsp4HI GCNGC 2 cut(s) 93, 639
GluI GCNGC 2 cut(s) 93, 639
GsuI CTGGAG 1 cut(s) 108
HaeIII GGCC 1 cut(s) 626
Hin1II CATG 2 cut(s) 599, 748
HinfI GANTC 6 cut(s) 146, 264, 491, 537, 677, 839
HphI GGTGA 1 cut(s) 913
Hpy166II GTNNAC 1 cut(s) 388
Hpy188I TCNGA 3 cut(s) 47, 274, 298
Hpy188III TCNNGA 2 cut(s) 512, 698
Hpy8I GTNNAC 1 cut(s) 388
HpyAV CCTTC 2 cut(s) 39, 240
HpyCH4IV ACGT 1 cut(s) 390
HpyCH4V TGCA 3 cut(s) 419, 524, 855
HpyF10VI GCNNNNNNNGC 2 cut(s) 134, 530
HpyF3I CTNAG 3 cut(s) 271, 443, 657
HpySE526I ACGT 1 cut(s) 390
Hsp92II CATG 2 cut(s) 599, 748
Kzo9I GATC 3 cut(s) 298, 478, 747
LmnI GCTCC 2 cut(s) 447, 530
Lsp1109I GCAGC 2 cut(s) 79, 650
LweI GCATC 2 cut(s) 782, 874
MaeII ACGT 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 830, 857
MalI GATC 3 cut(s) 300, 480, 749
MboI GATC 3 cut(s) 298, 478, 747
MboII GAAGA 4 cut(s) 17, 273, 304, 640
MflI RGATCY 1 cut(s) 478
MhlI GDGCHC 1 cut(s) 452
MluCI AATT 5 cut(s) 21, 199, 302, 502, 553
MlyI GAGTC 3 cut(s) 273, 546, 833
MmeI TCCRAC 1 cut(s) 382
MslI CAYNNNNRTG 3 cut(s) 185, 568, 926
MspA1I CMGCKG 1 cut(s) 128
MspR9I CCNGG 1 cut(s) 846
Mva1269I GAATGC 1 cut(s) 240
MvaI CCWGG 1 cut(s) 846
MwoI GCNNNNNNNGC 2 cut(s) 134, 530
NdeII GATC 3 cut(s) 298, 478, 747
NlaIII CATG 2 cut(s) 599, 748
NlaIV GGNNCC 1 cut(s) 917
NmeAIII GCCGAG 1 cut(s) 652
NmuCI GTSAC 1 cut(s) 857
OliI CACNNNNGTG 1 cut(s) 568
PcsI WCGNNNNNNNCGW 1 cut(s) 396
PctI GAATGC 1 cut(s) 240
PfeI GAWTC 3 cut(s) 146, 491, 677
PflMI CCANNNNNTGG 2 cut(s) 212, 566
PkrI GCNGC 2 cut(s) 94, 640
PleI GAGTC 3 cut(s) 272, 545, 833
PpsI GAGTC 3 cut(s) 272, 545, 833
PsiI TTATAA 1 cut(s) 949
Psp124BI GAGCTC 1 cut(s) 452
Psp6I CCWGG 1 cut(s) 844
PspGI CCWGG 1 cut(s) 844
PspN4I GGNNCC 1 cut(s) 917
PspPI GGNCC 1 cut(s) 821
PsuI RGATCY 1 cut(s) 478
RsaI GTAC 2 cut(s) 850, 872
RsaNI GTAC 2 cut(s) 849, 871
RseI CAYNNNNRTG 3 cut(s) 185, 568, 926
SacI GAGCTC 1 cut(s) 452
SatI GCNGC 2 cut(s) 93, 639
Sau3AI GATC 3 cut(s) 298, 478, 747
Sau96I GGNCC 1 cut(s) 821
SchI GAGTC 3 cut(s) 273, 546, 833
ScrFI CCNGG 1 cut(s) 846
SduI GDGCHC 1 cut(s) 452
SfaNI GCATC 2 cut(s) 782, 874
SfcI CTRYAG 2 cut(s) 240, 339
SinI GGWCC 1 cut(s) 821
SmiMI CAYNNNNRTG 3 cut(s) 185, 568, 926
Sse9I AATT 5 cut(s) 21, 199, 302, 502, 553
SsiI CCGC 1 cut(s) 128
SspI AATATT 1 cut(s) 153
SstI GAGCTC 1 cut(s) 452
StyD4I CCNGG 1 cut(s) 844
TaiI ACGT 1 cut(s) 393
TaqI TCGA 1 cut(s) 489
TasI AATT 5 cut(s) 21, 199, 302, 502, 553
TfiI GAWTC 3 cut(s) 146, 491, 677
TscAI CASTG 3 cut(s) 447, 531, 862
TseFI GTSAC 1 cut(s) 857
TseI GCWGC 2 cut(s) 92, 638
Tsp45I GTSAC 1 cut(s) 857
TspDTI ATGAA 2 cut(s) 27, 279
TspGWI ACGGA 2 cut(s) 388, 914
TspRI CASTG 3 cut(s) 447, 531, 862
Van91I CCANNNNNTGG 2 cut(s) 212, 566
VpaK11BI GGWCC 1 cut(s) 821
XapI RAATTY 2 cut(s) 21, 502
XmiI GTMKAC 1 cut(s) 387
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.