Rw6G037700

GDSL esterase lipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0000393
Physical Location & Seq
Forward (+)
62006934 .. 62008873
1940 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G037700.1

Sequence Viewer

Length: 1068 bp
ATGACTTACAATGTCCTATGGTTGTTTCTTTCTCTGGATCTGATATTTACCAGTGCCCTAGCTATTGTTAAGCTACCGAAAAATGCAACAGTTCCTGCTGTTTTTATGTTCGGAGATTCGATAGTCGACACAGGCAACAATAACAACCTAACAACCATTATAAAAAGCAATTTCCCTCCCTACGGAAGAGACTTTCCCGGAGGAGTTCCAACCGGAAGATTTAGCAACGGCAAGGTTGCATCCGACTTCGTTGTGGAGGAACTGGGGATCAAAGAATACTTGCCAGCCTATCTTGATCCAGATCTACAAGAGACAGAATTGCTTACTGGAGTAAACTTTGCTTCAAGCGGCTCAGGGTATGATCCCTTGACATCTACAATTATGAATGCTATGCCATTATCAAAACAGTTAGACATGTTGAAGGAATGCATTGAGAAGCTGAACAAGTATGTCGGAGAAGAGAAGGCGAACAGCATTATAAGCAACAGCTTTTTCATGGTGGAAACTGGAAGTGATGACCTTGTGAATACCTACTATCGTACACCTACACGTTTCTTTCAGTATGATATTTATGCCTACACTGATCTCCTTCTCACCGAGGCTTCGGCATTTGTGCAGAAATTATATGAATTGGGAGCACGGAGGATAGGGATCATAAGTCTGCCACCAGTTGGATGTATACCGTCACAGAGAACATTAGCAGGAGGTACAGAAAGAAGGTGCGTCGAGAAGTACAACGAAGCGACTGAGCTATTCAACACCAAGTTATCAGCGATGGCGGATTTTCAGACCAGTAACCTACCAGATGCCAGAGTCGTCTTCATGGATGCATACAATCCTTTCCTTCATATCCTCCAACACATACACAAATATGGATTTGAAGTTGTGGATAAAGGGTGTTGTGGAACAGGGATGATAGAGGTGATCTTTCTTTGTAATAGATGGGCTCCCAACACCTGTACAAACGTGTCGAGGTATGTGTTTTGGGACAGTTATCATCCTACGGAAAAAGCATATCAGATTCATACCCATCAAGTACTCCAAAACTCCATCCATTCTTTCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

355

Amino Acids

39.92

Weight (kDa)

5.31

Isoelectric Point (pI)

35.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipase_GDSL PF00657 34 - 342 1.2e-32 GDSL-like Lipase/Acylhydrolase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000393)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G20120 AT1G20132
fragaria_vesca FvH4_1g11330 FvH4_1g11340 FvH4_1g11341 FvH4_1g11342 FvH4_1g11390 FvH4_1g11390 FvH4_2g38111 FvH4_2g38111 FvH4_2g38111 FvH4_5g34670
malus_domestica MD02G1126900.v1.1 MD02G1127000.v1.1 MD02G1127100.v1.1 MD15G1008700.v1.1 MD15G1241600.v1.1 MD15G1241700.v1.1
prunus_persica Prupe.1G361600_v2.0.a1 Prupe.1G361700_v2.0.a1 Prupe.7G172300_v2.0.a1
pyrus_communis pycom02g09900 pycom02g09910 pycom02g09920 pycom15g00720 pycom15g21320
rosa_chinensis RchiOBHm_Chr2g0098851 RchiOBHm_Chr2g0098861 RchiOBHm_Chr2g0098871 RchiOBHm_Chr2g0098881 RchiOBHm_Chr2g0098921 RchiOBHm_Chr2g0130201 RchiOBHm_Chr6g0303431 RchiOBHm_Chr7g0235831
rosa_laevigata RLG00000001135 RLG00000011086 RLG00000016830 RLG00000016834 RLG00000019130
rosa_multiflora Rmu_co8160810.1_g000001 Rmu_co8282213.1_g000001 Rmu_co8476509.1_g000001 Rmu_sc0007686.1_g000001 Rmu_sc0008564.1_g000006 Rmu_sc0008564.1_g000009 Rmu_sc0008564.1_g000011 Rmu_sc0008564.1_g000016 Rmu_sc0008940.1_g000006 Rmu_sc0008941.1_g000009 Rmu_sc0010460.1_g000019 Rmu_sc0027015.1_g000001 Rmu_sc0027115.1_g000001 Rmu_sc0039572.1_g000001 Rmu_ssc0000114.1_g000053
rosa_roxburghii Rroxscaffold_2G00143590 Rroxscaffold_2G00143660 Rroxscaffold_2G00143670 Rroxscaffold_2G00143680 Rroxscaffold_7G00164780
rosa_rugosa Rorug02G0075000 Rorug02G0075100 Rorug02G0075200 Rorug02G0291100 Rorug02G0291200 Rorug02G0291300
rosa_samantha Rh2AG123000 Rh2AG123100 Rh2AG123200 Rh2AG123400 Rh2AG123800 Rh2AG123900 Rh2AG124200 Rh2AG124400 Rh2AG343000 Rh2BG126600 Rh2BG126700 Rh2BG126900 Rh2BG127100 Rh2BG127400 Rh2BG127600 Rh2BG350800 Rh2CG127600 Rh2CG127700 Rh2CG127800 Rh2CG128000 Rh2CG128300 Rh2CG128500 Rh2CG330100 Rh6AG435600 Rh6CG447600 Rh6DG434300 Rh7AG447900 Rh7BG420100 Rh7CG468400
rosa_wichuraiana Rw0G004290 Rw2G009510 Rw2G009550 Rw2G027630 Rw2G027650 Rw6G037700 Rw7G037230

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 161, 479
AccB7I CCANNNNNTGG 1 cut(s) 671
AccI GTMKAC 2 cut(s) 126, 679
AciI CCGC 2 cut(s) 348, 779
AclWI GGATC 5 cut(s) 45, 275, 290, 356, 659
AfaI GTAC 5 cut(s) 541, 709, 734, 961, 1038
AfiI CCNNNNNNNGG 2 cut(s) 182, 671
AflIII ACRYGT 3 cut(s) 414, 548, 966
AgsI TTSAA 4 cut(s) 345, 421, 757, 881
AluBI AGCT 5 cut(s) 62, 73, 439, 489, 751
AluI AGCT 5 cut(s) 62, 73, 439, 489, 751
Alw21I GWGCWC 1 cut(s) 640
Alw26I GTCTC 2 cut(s) 183, 305
AlwI GGATC 5 cut(s) 45, 275, 290, 356, 659
AlwNI CAGNNNCTG 1 cut(s) 95
AsuC2I CCSGG 1 cut(s) 198
AsuHPI GGTGA 2 cut(s) 586, 934
BaeGI GKGCMC 1 cut(s) 58
BanII GRGCYC 1 cut(s) 949
BbsI GAAGAC 1 cut(s) 811
Bbv12I GWGCWC 1 cut(s) 640
BccI CCATC 4 cut(s) 769, 936, 1038, 1058
BceAI ACGGC 1 cut(s) 244
BcnI CCSGG 1 cut(s) 198
BcoDI GTCTC 2 cut(s) 183, 305
BfaI CTAG 1 cut(s) 59
BglII AGATCT 1 cut(s) 301
BisI GCNGC 1 cut(s) 349
BlsI GCNGC 1 cut(s) 350
BmcAI AGTACT 1 cut(s) 1038
Bme1390I CCNGG 1 cut(s) 198
BmiI GGNNCC 1 cut(s) 948
BmrFI CCNGG 1 cut(s) 198
BmrI ACTGGG 1 cut(s) 272
BmsI GCATC 3 cut(s) 248, 796, 817
BmuI ACTGGG 1 cut(s) 272
BpiI GAAGAC 1 cut(s) 811
BpmI CTGGAG 1 cut(s) 348
Bpu10I CCTNAGC 1 cut(s) 352
BpuMI CCSGG 1 cut(s) 198
BsaBI GATNNNNATC 2 cut(s) 300, 650
BsaJI CCNNGG 1 cut(s) 597
BsaWI WCCGGW 1 cut(s) 212
Bsc4I CCNNNNNNNGG 2 cut(s) 182, 671
Bse1I ACTGG 6 cut(s) 51, 267, 331, 511, 668, 792
Bse8I GATNNNNATC 2 cut(s) 300, 650
BseDI CCNNGG 1 cut(s) 597
BseGI GGATG 6 cut(s) 239, 680, 832, 918, 997, 1050
BseJI GATNNNNATC 2 cut(s) 300, 650
BseLI CCNNNNNNNGG 2 cut(s) 182, 671
BseMII CTCAG 2 cut(s) 366, 738
BseNI ACTGG 6 cut(s) 51, 267, 331, 511, 668, 792
BseRI GAGGAG 1 cut(s) 216
BseSI GKGCMC 1 cut(s) 58
BsgI GTGCAG 1 cut(s) 635
BsiHKAI GWGCWC 1 cut(s) 640
BsiSI CCGG 2 cut(s) 198, 213
BslFI GGGAC 1 cut(s) 1001
BslI CCNNNNNNNGG 2 cut(s) 182, 671
BsmAI GTCTC 2 cut(s) 183, 305
BsmFI GGGAC 1 cut(s) 1001
BsmI GAATGC 2 cut(s) 391, 431
Bsp1286I GDGCHC 3 cut(s) 58, 640, 949
Bsp1407I TGTACA 1 cut(s) 959
Bsp143I GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
BspACI CCGC 2 cut(s) 348, 779
BspCNI CTCAG 2 cut(s) 365, 739
BspLI GGNNCC 1 cut(s) 948
BspPI GGATC 5 cut(s) 45, 275, 290, 356, 659
BsrGI TGTACA 1 cut(s) 959
BsrI ACTGG 6 cut(s) 51, 267, 331, 511, 668, 792
BssECI CCNNGG 1 cut(s) 597
BssMI GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
BssNAI GTATAC 1 cut(s) 680
Bst1107I GTATAC 1 cut(s) 680
Bst4CI ACNGT 4 cut(s) 91, 408, 684, 992
Bst6I CTCTTC 2 cut(s) 181, 453
BstAUI TGTACA 1 cut(s) 959
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 352, 747
BstF5I GGATG 6 cut(s) 239, 680, 832, 918, 997, 1050
BstKTI GATC 8 cut(s) 40, 270, 298, 304, 364, 586, 654, 927
BstMAI GTCTC 2 cut(s) 183, 305
BstMBI GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
BstMWI GCNNNNNNNGC 1 cut(s) 480
BstNSI RCATGY 1 cut(s) 418
BstSCI CCNGG 1 cut(s) 196
BstSLI GKGCMC 1 cut(s) 58
BstV2I GAAGAC 1 cut(s) 811
BstX2I RGATCY 2 cut(s) 37, 301
BstYI RGATCY 2 cut(s) 37, 301
BstZ17I GTATAC 1 cut(s) 680
BtgZI GCGATG 1 cut(s) 788
BtsCI GGATG 6 cut(s) 239, 680, 832, 918, 997, 1050
BtsIMutI CAGTG 2 cut(s) 58, 579
Cac8I GCNNGC 1 cut(s) 285
CaiI CAGNNNCTG 1 cut(s) 95
CseI GACGC 1 cut(s) 712
Csp6I GTAC 5 cut(s) 540, 708, 733, 960, 1037
CviAII CATG 3 cut(s) 415, 496, 823
CviJI RGCY 9 cut(s) 62, 73, 287, 351, 439, 489, 602, 751, 947
CviKI_1 RGCY 9 cut(s) 62, 73, 287, 351, 439, 489, 602, 751, 947
CviQI GTAC 5 cut(s) 540, 708, 733, 960, 1037
DdeI CTNAG 2 cut(s) 352, 747
DpnI GATC 8 cut(s) 39, 269, 297, 303, 363, 585, 653, 926
DpnII GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
Eam1104I CTCTTC 2 cut(s) 181, 453
EarI CTCTTC 2 cut(s) 181, 453
EciI GGCGGA 1 cut(s) 794
Eco24I GRGCYC 1 cut(s) 949
EcoT22I ATGCAT 2 cut(s) 431, 832
EcoT38I GRGCYC 1 cut(s) 949
FaeI CATG 3 cut(s) 418, 499, 826
FaqI GGGAC 1 cut(s) 1001
FatI CATG 3 cut(s) 414, 495, 822
FblI GTMKAC 2 cut(s) 126, 679
Fnu4HI GCNGC 1 cut(s) 349
FokI GGATG 6 cut(s) 226, 687, 839, 925, 984, 1037
FriOI GRGCYC 1 cut(s) 949
Fsp4HI GCNGC 1 cut(s) 349
FspBI CTAG 1 cut(s) 59
GluI GCNGC 1 cut(s) 349
GsuI CTGGAG 1 cut(s) 348
HapII CCGG 2 cut(s) 198, 213
HgaI GACGC 1 cut(s) 712
Hin1II CATG 3 cut(s) 418, 499, 826
HincII GTYRAC 1 cut(s) 127
HindII GTYRAC 1 cut(s) 127
HinfI GANTC 3 cut(s) 116, 813, 1021
HpaII CCGG 2 cut(s) 198, 213
HphI GGTGA 2 cut(s) 586, 934
Hpy166II GTNNAC 4 cut(s) 127, 334, 542, 680
Hpy188I TCNGA 7 cut(s) 42, 113, 244, 455, 789, 1020, 1067
Hpy188III TCNNGA 4 cut(s) 35, 293, 299, 727
Hpy8I GTNNAC 4 cut(s) 127, 334, 542, 680
Hpy99I CGWCG 1 cut(s) 728
HpyAV CCTTC 5 cut(s) 415, 457, 599, 711, 854
HpyCH4III ACNGT 4 cut(s) 91, 408, 684, 992
HpyCH4IV ACGT 2 cut(s) 550, 966
HpyCH4V TGCA 5 cut(s) 86, 239, 429, 616, 830
HpyF10VI GCNNNNNNNGC 1 cut(s) 480
HpyF3I CTNAG 2 cut(s) 352, 747
HpySE526I ACGT 2 cut(s) 550, 966
Hsp92II CATG 3 cut(s) 418, 499, 826
Kzo9I GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
LmnI GCTCC 2 cut(s) 635, 952
LweI GCATC 3 cut(s) 248, 796, 817
MaeI CTAG 1 cut(s) 59
MaeII ACGT 2 cut(s) 550, 966
MaeIII GTNAC 2 cut(s) 684, 794
MalI GATC 8 cut(s) 39, 269, 297, 303, 363, 585, 653, 926
MboI GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
MboII GAAGA 5 cut(s) 198, 228, 470, 811, 1054
MflI RGATCY 2 cut(s) 37, 301
MhlI GDGCHC 3 cut(s) 58, 640, 949
MluCI AATT 5 cut(s) 169, 317, 378, 620, 629
MlyI GAGTC 1 cut(s) 822
MmeI TCCRAC 5 cut(s) 233, 267, 433, 652, 880
MnlI CCTC 9 cut(s) 186, 194, 250, 592, 636, 698, 863, 913, 966
Mph1103I ATGCAT 2 cut(s) 431, 832
MseI TTAA 1 cut(s) 69
MslI CAYNNNNRTG 1 cut(s) 870
MspI CCGG 2 cut(s) 198, 213
MspR9I CCNGG 1 cut(s) 198
Mva1269I GAATGC 2 cut(s) 391, 431
MwoI GCNNNNNNNGC 1 cut(s) 480
NciI CCSGG 1 cut(s) 198
NdeII GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
NlaIII CATG 3 cut(s) 418, 499, 826
NlaIV GGNNCC 1 cut(s) 948
NmuCI GTSAC 1 cut(s) 684
NsiI ATGCAT 2 cut(s) 431, 832
NspI RCATGY 1 cut(s) 418
PciI ACATGT 1 cut(s) 414
PctI GAATGC 2 cut(s) 391, 431
PfeI GAWTC 2 cut(s) 116, 1021
PflMI CCANNNNNTGG 1 cut(s) 671
PfoI TCCNGGA 1 cut(s) 196
PkrI GCNGC 1 cut(s) 350
PleI GAGTC 1 cut(s) 821
PpsI GAGTC 1 cut(s) 821
PscI ACATGT 1 cut(s) 414
PsiI TTATAA 2 cut(s) 161, 479
PspN4I GGNNCC 1 cut(s) 948
PstNI CAGNNNCTG 1 cut(s) 95
PsuI RGATCY 2 cut(s) 37, 301
RsaI GTAC 5 cut(s) 541, 709, 734, 961, 1038
RsaNI GTAC 5 cut(s) 540, 708, 733, 960, 1037
RseI CAYNNNNRTG 1 cut(s) 870
SalI GTCGAC 1 cut(s) 125
SaqAI TTAA 1 cut(s) 69
SatI GCNGC 1 cut(s) 349
Sau3AI GATC 8 cut(s) 37, 267, 295, 301, 361, 583, 651, 924
ScaI AGTACT 1 cut(s) 1038
SchI GAGTC 1 cut(s) 822
ScrFI CCNGG 1 cut(s) 198
SduI GDGCHC 3 cut(s) 58, 640, 949
SfaNI GCATC 3 cut(s) 248, 796, 817
SmiMI CAYNNNNRTG 1 cut(s) 870
Sse9I AATT 5 cut(s) 169, 317, 378, 620, 629
SsiI CCGC 2 cut(s) 348, 779
SspMI CTAG 1 cut(s) 59
StyD4I CCNGG 1 cut(s) 196
TaaI ACNGT 4 cut(s) 91, 408, 684, 992
TaiI ACGT 2 cut(s) 553, 969
TaqI TCGA 4 cut(s) 119, 126, 726, 971
TasI AATT 5 cut(s) 169, 317, 378, 620, 629
TatI WGTACW 3 cut(s) 732, 959, 1036
TauI GCSGC 1 cut(s) 351
TfiI GAWTC 2 cut(s) 116, 1021
Tru1I TTAA 1 cut(s) 69
Tru9I TTAA 1 cut(s) 69
TscAI CASTG 2 cut(s) 58, 586
TseFI GTSAC 1 cut(s) 684
Tsp45I GTSAC 1 cut(s) 684
TspDTI ATGAA 6 cut(s) 398, 484, 642, 811, 836, 1013
TspGWI ACGGA 3 cut(s) 198, 655, 1019
TspRI CASTG 2 cut(s) 58, 586
Van91I CCANNNNNTGG 1 cut(s) 671
XceI RCATGY 1 cut(s) 418
XmiI GTMKAC 2 cut(s) 126, 679
XspI CTAG 1 cut(s) 59
ZrmI AGTACT 1 cut(s) 1038
Zsp2I ATGCAT 2 cut(s) 431, 832
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.