Rh2BG350600

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0010038
Physical Location & Seq
Forward (+)
47598387 .. 47603379
4993 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG350600.1

Sequence Viewer

Length: 990 bp
ATGGCAGCCCTGAAGCTCCTCCTCTCCTCGGCTCGCCGTCACTGCTCCCATCTCCACCCGTCTCCATTTCTAAGGTCCCTCACCTCCCAATCAGATCAAATTCCCTCTCCTCCCGAACCCCTTAAACCCCAACCCGCTCCGATCCAACCCGTCTCCTACGCTCCCAAACCCAAACAACACTCCTCCTCACCCGACCCGGACCCGGACCCGGCTCCGCAATCAGCACAAACACAACCGCCGCCACCGTCGCACGCGCCTCCTCACGACTCGCCGTGGACCCGAGAAGACGCCCGGTTCGTGAAGGACGGGCCTTCCATAACCCCGGTGTCGTACCCGGCCCGAGTGGCTCCGTTGCCGGAAGATAAGGTCTCGGAGGAAGGCGGCGACGCGAGGCAGGGTGAGCTGGAGAAGGAGAAGCAGAGGATCGAGCAGTCGAGAAGGAGGGTTTTTAGGGTAGAGGAGAATGTGGTGGTTCCTTTCCCCACGTTTCTTAAGGTGGAGAAGAAGGAAAGGAAGCCCATTCTTGATTTGATGGAAGCAATTCGACAAGTCAAGGCTACTGCCAAGTGCAACTTTGATGAAACTGTGGAAGCTCATGTGCAATTGGGTATTGATGCCAAGCGAAATGCAGTTCGTGGCAACATGACCTTGCCCCATGGTAGCGGAAAGCTAGGTACTGTGACTAACGACATTGCTGGGGCACTACAAAAAGTAAGACAAGGTCATATTGAGTATAAAATGGACAGAACATCAATAGTTCATGTTGGGCTCGGAAAGGTAAGCTTTACAGAGGAGTATCTGCGTGAGAATGTTGGTGCATTTATGAGTTCTCTTTTGCTGGCAAAGCCTACGGGTTTAAAAAAGTCTTCCAAATATGCTGGGTATATCAACTCTTTCCACATATGTAGCACAATGGGCTCCGGATTTCCAGTTTCAATACAGTCATTATCCAAAGCTGCAGATAACTACAATAAAATGCACCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

329

Amino Acids

36.26

Weight (kDa)

9.41

Isoelectric Point (pI)

68.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 224 - 306 6e-16 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 137
AccII CGCG 2 cut(s) 254, 389
AccIII TCCGGA 1 cut(s) 920
AciI CCGC 6 cut(s) 135, 215, 236, 239, 381, 663
AclWI GGATC 2 cut(s) 136, 431
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 32
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 332, 676
AfiI CCNNNNNNNGG 3 cut(s) 28, 202, 208
AflII CTTAAG 1 cut(s) 491
AgsI TTSAA 1 cut(s) 936
AluBI AGCT 6 cut(s) 16, 403, 593, 670, 783, 956
AluI AGCT 6 cut(s) 16, 403, 593, 670, 783, 956
Alw26I GTCTC 3 cut(s) 66, 157, 373
AlwI GGATC 2 cut(s) 136, 431
Ama87I CYCGRG 2 cut(s) 279, 339
Aor13HI TCCGGA 1 cut(s) 920
AoxI GGCC 2 cut(s) 308, 336
ApeKI GCWGC 2 cut(s) 5, 956
ApoI RAATTY 1 cut(s) 99
ArsI GACNNNNNNTTYG 2 cut(s) 278, 310
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
AsuC2I CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
AsuHPI GGTGA 3 cut(s) 73, 180, 410
AvaI CYCGRG 2 cut(s) 279, 339
AvaII GGWCC 4 cut(s) 75, 199, 205, 276
BaeGI GKGCMC 1 cut(s) 703
BanII GRGCYC 2 cut(s) 771, 920
BbsI GAAGAC 2 cut(s) 291, 858
BbvI GCAGC 2 cut(s) 17, 943
BccI CCATC 2 cut(s) 57, 526
BceAI ACGGC 2 cut(s) 21, 256
BcnI CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
BcoDI GTCTC 3 cut(s) 66, 157, 373
BfaI CTAG 1 cut(s) 671
BfmI CTRYAG 1 cut(s) 957
BfrI CTTAAG 1 cut(s) 491
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 4 cut(s) 6, 239, 382, 957
BlsI GCNGC 4 cut(s) 7, 240, 383, 958
Bme1390I CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
Bme18I GGWCC 4 cut(s) 75, 199, 205, 276
BmeT110I CYCGRG 2 cut(s) 279, 339
BmgT120I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
BmiI GGNNCC 8 cut(s) 77, 201, 207, 213, 278, 348, 474, 919
BmrFI CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
BmsI GCATC 1 cut(s) 604
BpiI GAAGAC 2 cut(s) 291, 858
BpmI CTGGAG 1 cut(s) 425
BpuEI CTTGAG 1 cut(s) 968
BpuMI CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 4 cut(s) 27, 272, 321, 655
BsaWI WCCGGW 1 cut(s) 920
Bsc4I CCNNNNNNNGG 3 cut(s) 28, 202, 208
Bse1I ACTGG 1 cut(s) 929
Bse3DI GCAATG 1 cut(s) 690
BseAI TCCGGA 1 cut(s) 920
BseDI CCNNGG 4 cut(s) 27, 272, 321, 655
BseLI CCNNNNNNNGG 3 cut(s) 28, 202, 208
BseMI GCAATG 1 cut(s) 690
BseNI ACTGG 1 cut(s) 929
BseRI GAGGAG 9 cut(s) 8, 11, 16, 99, 172, 175, 249, 473, 806
BseSI GKGCMC 1 cut(s) 703
BseXI GCAGC 2 cut(s) 17, 943
BseYI CCCAGC 2 cut(s) 695, 878
Bsh1236I CGCG 2 cut(s) 254, 389
BshFI GGCC 2 cut(s) 310, 338
BsiHKCI CYCGRG 2 cut(s) 279, 339
BsiSI CCGG 8 cut(s) 197, 203, 209, 292, 323, 335, 356, 921
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 3 cut(s) 28, 202, 208
BsmAI GTCTC 3 cut(s) 66, 157, 373
BsmBI CGTCTC 2 cut(s) 66, 157
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 310, 338
Bso31I GGTCTC 1 cut(s) 373
BsoBI CYCGRG 2 cut(s) 279, 339
Bsp1286I GDGCHC 3 cut(s) 703, 771, 920
Bsp13I TCCGGA 1 cut(s) 920
Bsp143I GATC 3 cut(s) 94, 141, 423
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 6 cut(s) 135, 215, 236, 239, 381, 663
BspANI GGCC 2 cut(s) 310, 338
BspEI TCCGGA 1 cut(s) 920
BspFNI CGCG 2 cut(s) 254, 389
BspLI GGNNCC 8 cut(s) 77, 201, 207, 213, 278, 348, 474, 919
BspMAI CTGCAG 1 cut(s) 961
BspPI GGATC 2 cut(s) 136, 431
BspTI CTTAAG 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 690
BsrI ACTGG 1 cut(s) 929
BssECI CCNNGG 4 cut(s) 27, 272, 321, 655
BssMI GATC 3 cut(s) 94, 141, 423
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 655
Bst4CI ACNGT 4 cut(s) 246, 586, 679, 942
BstACI GRCGYC 1 cut(s) 288
BstAFI CTTAAG 1 cut(s) 491
BstC8I GCNNGC 3 cut(s) 34, 252, 840
BstDEI CTNAG 1 cut(s) 71
BstDSI CCRYGG 2 cut(s) 272, 655
BstFNI CGCG 2 cut(s) 254, 389
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 97, 144, 426
BstMAI GTCTC 3 cut(s) 66, 157, 373
BstMBI GATC 3 cut(s) 94, 141, 423
BstMWI GCNNNNNNNGC 6 cut(s) 42, 247, 344, 400, 844, 915
BstSCI CCNGG 6 cut(s) 195, 201, 207, 290, 321, 333
BstSFI CTRYAG 1 cut(s) 957
BstSLI GKGCMC 1 cut(s) 703
BstUI CGCG 2 cut(s) 254, 389
BstV1I GCAGC 2 cut(s) 17, 943
BstV2I GAAGAC 2 cut(s) 291, 858
BsuRI GGCC 2 cut(s) 310, 338
BtgI CCRYGG 2 cut(s) 272, 655
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 34, 252, 840
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
CseI GACGC 2 cut(s) 296, 395
Csp6I GTAC 2 cut(s) 331, 675
CviAII CATG 4 cut(s) 596, 643, 656, 761
CviQI GTAC 2 cut(s) 331, 675
DdeI CTNAG 1 cut(s) 71
DpnI GATC 3 cut(s) 96, 143, 425
DpnII GATC 3 cut(s) 94, 141, 423
DraI TTTAAA 1 cut(s) 858
Eco130I CCWWGG 1 cut(s) 655
Eco24I GRGCYC 2 cut(s) 771, 920
Eco31I GGTCTC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 75, 199, 205, 276
Eco57I CTGAAG 1 cut(s) 32
Eco88I CYCGRG 2 cut(s) 279, 339
EcoO109I RGGNCCY 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 655
EcoT38I GRGCYC 2 cut(s) 771, 920
ErhI CCWWGG 1 cut(s) 655
Esp3I CGTCTC 2 cut(s) 66, 157
FaeI CATG 4 cut(s) 599, 646, 659, 764
FalI AAGNNNNNCTT 4 cut(s) 557, 589, 767, 799
FaqI GGGAC 1 cut(s) 61
FatI CATG 4 cut(s) 595, 642, 655, 760
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 1 cut(s) 902
Fnu4HI GCNGC 4 cut(s) 6, 239, 382, 957
FriOI GRGCYC 2 cut(s) 771, 920
Fsp4HI GCNGC 4 cut(s) 6, 239, 382, 957
FspBI CTAG 1 cut(s) 671
GlaI GCGC 1 cut(s) 255
GluI GCNGC 4 cut(s) 6, 239, 382, 957
GsaI CCCAGC 2 cut(s) 699, 882
GsuI CTGGAG 1 cut(s) 425
HaeIII GGCC 2 cut(s) 310, 338
HapII CCGG 8 cut(s) 197, 203, 209, 292, 323, 335, 356, 921
HgaI GACGC 2 cut(s) 296, 395
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 4 cut(s) 599, 646, 659, 764
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HindIII AAGCTT 1 cut(s) 781
HinfI GANTC 1 cut(s) 266
HpaII CCGG 8 cut(s) 197, 203, 209, 292, 323, 335, 356, 921
HphI GGTGA 3 cut(s) 73, 180, 410
Hpy166II GTNNAC 1 cut(s) 276
Hpy188I TCNGA 4 cut(s) 94, 141, 373, 773
Hpy188III TCNNGA 6 cut(s) 113, 263, 298, 435, 524, 921
Hpy8I GTNNAC 1 cut(s) 276
Hpy99I CGWCG 2 cut(s) 250, 389
HpyAV CCTTC 6 cut(s) 295, 321, 371, 403, 432, 499
HpyCH4III ACNGT 4 cut(s) 246, 586, 679, 942
HpyCH4IV ACGT 1 cut(s) 485
HpyCH4V TGCA 6 cut(s) 570, 601, 629, 818, 959, 979
HpyF10VI GCNNNNNNNGC 6 cut(s) 42, 247, 344, 400, 844, 915
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 1 cut(s) 485
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 4 cut(s) 599, 646, 659, 764
HspAI GCGC 1 cut(s) 254
Kpn2I TCCGGA 1 cut(s) 920
Kzo9I GATC 3 cut(s) 94, 141, 423
LmnI GCTCC 7 cut(s) 21, 50, 142, 166, 217, 352, 923
Lsp1109I GCAGC 2 cut(s) 17, 943
LweI GCATC 1 cut(s) 604
MaeI CTAG 1 cut(s) 671
MaeII ACGT 1 cut(s) 485
MaeIII GTNAC 2 cut(s) 38, 679
MalI GATC 3 cut(s) 96, 143, 425
MbiI CCGCTC 1 cut(s) 137
MboI GATC 3 cut(s) 94, 141, 423
MboII GAAGA 4 cut(s) 296, 371, 514, 858
MfeI CAATTG 1 cut(s) 602
MhlI GDGCHC 3 cut(s) 703, 771, 920
MluCI AATT 3 cut(s) 99, 540, 602
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 169
MroI TCCGGA 1 cut(s) 920
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 3 cut(s) 123, 492, 857
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 8 cut(s) 197, 203, 209, 292, 323, 335, 356, 921
MspR9I CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
MunI CAATTG 1 cut(s) 602
MvnI CGCG 2 cut(s) 254, 389
MwoI GCNNNNNNNGC 6 cut(s) 42, 247, 344, 400, 844, 915
NciI CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
NcoI CCATGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 902
NdeII GATC 3 cut(s) 94, 141, 423
NlaIII CATG 4 cut(s) 599, 646, 659, 764
NlaIV GGNNCC 8 cut(s) 77, 201, 207, 213, 278, 348, 474, 919
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 38, 679
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 540
PflFI GACNNNGTC 1 cut(s) 720
PkrI GCNGC 4 cut(s) 7, 240, 383, 958
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
PspFI CCCAGC 2 cut(s) 695, 878
PspN4I GGNNCC 8 cut(s) 77, 201, 207, 213, 278, 348, 474, 919
PspPI GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
PspPPI RGGWCCY 1 cut(s) 75
PstI CTGCAG 1 cut(s) 961
PsyI GACNNNGTC 1 cut(s) 720
RsaI GTAC 2 cut(s) 332, 676
RsaNI GTAC 2 cut(s) 331, 675
SaqAI TTAA 3 cut(s) 123, 492, 857
SatI GCNGC 4 cut(s) 6, 239, 382, 957
Sau3AI GATC 3 cut(s) 94, 141, 423
Sau96I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
SduI GDGCHC 3 cut(s) 703, 771, 920
SfaNI GCATC 1 cut(s) 604
SfcI CTRYAG 1 cut(s) 957
SinI GGWCC 4 cut(s) 75, 199, 205, 276
SmlI CTYRAG 2 cut(s) 491, 983
SmoI CTYRAG 2 cut(s) 491, 983
Sse9I AATT 3 cut(s) 99, 540, 602
SsiI CCGC 6 cut(s) 135, 215, 236, 239, 381, 663
SspMI CTAG 1 cut(s) 671
StyD4I CCNGG 6 cut(s) 195, 201, 207, 290, 321, 333
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 4 cut(s) 246, 586, 679, 942
TaiI ACGT 1 cut(s) 488
TaqI TCGA 3 cut(s) 426, 434, 544
TasI AATT 3 cut(s) 99, 540, 602
TauI GCSGC 2 cut(s) 241, 384
Tru1I TTAA 3 cut(s) 123, 492, 857
Tru9I TTAA 3 cut(s) 123, 492, 857
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 38, 679
TseI GCWGC 2 cut(s) 5, 956
Tsp45I GTSAC 2 cut(s) 38, 679
TspDTI ATGAA 2 cut(s) 594, 749
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 1 cut(s) 47
Tth111I GACNNNGTC 1 cut(s) 720
Vha464I CTTAAG 1 cut(s) 491
VpaK11BI GGWCC 4 cut(s) 75, 199, 205, 276
XapI RAATTY 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 1 cut(s) 671
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.