MD11G1004300.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0010038
Physical Location & Seq
Reverse (-)
372929 .. 378061
5133 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1004300.v1.1.491

Sequence Viewer

Length: 1284 bp
ATGGCCGCCCTGAAGCTCCTCCTCTCCTCCGCTCGCCGTCACTGCCTCTCAAACCCTCACTCCCGTCTCCACCCACCTCCATTTCTAAGCTGCCACAGATCCCTAAGCTCCGAATCCGACCCAAACCCGAATCAGAACCCTCCGCAGCCTCCTAGACGCCAACCCGTGCCGGTCGAACCCGTTTCATACACTCTCAAACCCAAAGAGCAGTCCCTACCCGATCCAGATGCGACTCCCCAAGCAACATCACCGCCGCCTCCACAAAGCCCCGGTGACTCGCCCAACTCATCGAGTCAGGACACTCGCGCCACCTGGACCCGTGAAGAGGCGCGTTTCATCAGGGACGTTCCATCTATATCTCCGGTATCGTACCCGGTGAAAGTTGCTCCCTTACCGGAAGATAGGGTCGCGGAGGAGAGCGGAGGTGGCGAAGGCGGAGCTGAAAAGGATGTGGATGGCGTGAAGGACTTGGAGAGAGGGAAGAGAAGGATCGAGGCCGATGGTCGGATGAGGAGGAGGCTTTTTAGGGTTCCTGAGGAGGAGGTGGTGGTTCCTTTTCCCGCGTTGATTAAGGTTGAGAAGAAGGAGAGCAAATCTATTCTCGAGGTCATGGACGCAATTCGCCAAGTCAAGGCTAATGCCCAGTGCAAATTTGATGAAACTGTTGAAGCTCATGTACAATTGGGTATTGATGCGAAGCGAAATGCGGTTCGTGGTAACATGACTTTGCCGCATGGTAGTGGGAAGGTTGTCAGGGTTGCTTTCTTTGCTGAAGGCTCAGATGCAGAAGAAGCTAGAGCTGCAGGAGCAGATATTGTTGGCGGTGTTGAACTGGTTGAGGAAATTGCAAGCAGTCATAAGCTCAATGTTGACAAGTGTTTTGCTACAAATGAAATGATTATGCGCCTGGCAAAGATTGCAAGAATTCTTAGAGAGCGTGGTTTGATGCCTGACCGTAAACTAGGTACTGTCACCAATGACATTACTGGAGCACTGCAGAAGGTAAGACAAGGTCATATTGAGTATAGAATGGACAGAACATCAATTGTACATGTGGGTCTTGGAAAGGTGAGCTTCACAGAGGAGTATCTGCAAGAGAACATTGGTGCATTTATGAGTTCTCTTTTGCTGGCAAAGCCTACAGGTTTAAAAAAGTCTTCCAAATATGCGGGGTATATTAACTCGTTCCATATAAGTAGCACGATGGGTCCAGGATTCCCGGTTTCAATACAGTCATTGTCCAAAGCTGCAGATCACTACAATAAAGTGCACCTCAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

428

Amino Acids

46.82

Weight (kDa)

9.08

Isoelectric Point (pI)

61.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 211 - 404 3.7e-47 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 32, 420
AccII CGCG 4 cut(s) 306, 331, 410, 563
AclWI GGATC 3 cut(s) 93, 215, 497
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 2 cut(s) 650, 924
AcuI CTGAAG 2 cut(s) 32, 792
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 4 cut(s) 371, 678, 967, 1050
AfiI CCNNNNNNNGG 3 cut(s) 325, 504, 631
AflIII ACRYGT 1 cut(s) 1051
AgsI TTSAA 3 cut(s) 668, 830, 1227
AjnI CCWGG 3 cut(s) 311, 906, 1210
Alw21I GWGCWC 2 cut(s) 994, 1272
Alw26I GTCTC 1 cut(s) 71
Alw44I GTGCAC 1 cut(s) 1268
AlwI GGATC 3 cut(s) 93, 215, 497
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 2 cut(s) 3, 495
ApaLI GTGCAC 1 cut(s) 1268
ApeKI GCWGC 4 cut(s) 90, 145, 800, 1247
ApoI RAATTY 2 cut(s) 650, 924
ArsI GACNNNNNNTTYG 2 cut(s) 863, 895
AspLEI GCGC 3 cut(s) 308, 331, 906
AspS9I GGNCC 2 cut(s) 315, 1208
AsuC2I CCSGG 3 cut(s) 270, 374, 1220
AsuHPI GGTGA 5 cut(s) 240, 284, 388, 964, 1081
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 2 cut(s) 315, 1208
AxyI CCTNAGG 1 cut(s) 534
BaeGI GKGCMC 1 cut(s) 1272
BarI GAAGNNNNNNTAC 2 cut(s) 660, 692
BbsI GAAGAC 1 cut(s) 1149
Bbv12I GWGCWC 2 cut(s) 994, 1272
BbvI GCAGC 4 cut(s) 77, 157, 787, 1234
BccI CCATC 4 cut(s) 358, 449, 494, 1198
BceAI ACGGC 1 cut(s) 21
BcgI CGANNNNNNTGC 2 cut(s) 209, 243
BciT130I CCWGG 3 cut(s) 313, 908, 1212
BcnI CCSGG 3 cut(s) 270, 374, 1220
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 3 cut(s) 153, 795, 962
BfmI CTRYAG 4 cut(s) 801, 995, 1140, 1248
BisI GCNGC 7 cut(s) 6, 91, 146, 254, 731, 801, 1248
BlsI GCNGC 7 cut(s) 7, 92, 147, 255, 732, 802, 1249
Bme1390I CCNGG 6 cut(s) 270, 313, 374, 908, 1212, 1220
Bme18I GGWCC 2 cut(s) 315, 1208
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 315, 1208
BmiI GGNNCC 4 cut(s) 317, 531, 552, 1209
BmrFI CCNGG 6 cut(s) 270, 313, 374, 908, 1212, 1220
BmrI ACTGGG 1 cut(s) 637
BmsI GCATC 4 cut(s) 217, 682, 772, 936
BmuI ACTGGG 1 cut(s) 637
BpiI GAAGAC 1 cut(s) 1149
BpmI CTGGAG 1 cut(s) 1008
Bpu10I CCTNAGC 1 cut(s) 104
BpuEI CTTGAG 1 cut(s) 1259
BpuMI CCSGG 3 cut(s) 270, 374, 1220
BsaHI GRCGYC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 268
BsaWI WCCGGW 2 cut(s) 361, 394
BsaXI ACNNNNNCTCC 2 cut(s) 44, 74
Bsc4I CCNNNNNNNGG 3 cut(s) 325, 504, 631
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 3 cut(s) 643, 837, 991
Bse21I CCTNAGG 1 cut(s) 534
BseBI CCWGG 3 cut(s) 313, 908, 1212
BseDI CCNNGG 1 cut(s) 268
BseGI GGATG 3 cut(s) 454, 460, 513
BseLI CCNNNNNNNGG 3 cut(s) 325, 504, 631
BseMII CTCAG 2 cut(s) 525, 792
BseNI ACTGG 3 cut(s) 643, 837, 991
BseRI GAGGAG 9 cut(s) 8, 11, 16, 428, 526, 529, 551, 554, 1097
BseSI GKGCMC 1 cut(s) 1272
BseXI GCAGC 4 cut(s) 77, 157, 787, 1234
Bsh1236I CGCG 4 cut(s) 306, 331, 410, 563
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 2 cut(s) 5, 497
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 994, 1272
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 6 cut(s) 170, 270, 362, 374, 395, 1220
BslFI GGGAC 2 cut(s) 196, 356
BslI CCNNNNNNNGG 3 cut(s) 325, 504, 631
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 2 cut(s) 196, 356
BsnI GGCC 2 cut(s) 5, 497
BsoBI CYCGRG 1 cut(s) 602
Bsp1286I GDGCHC 2 cut(s) 994, 1272
Bsp1407I TGTACA 2 cut(s) 676, 1048
Bsp143I GATC 4 cut(s) 98, 220, 489, 1252
BspANI GGCC 2 cut(s) 5, 497
BspCNI CTCAG 2 cut(s) 526, 791
BspFNI CGCG 4 cut(s) 306, 331, 410, 563
BspLI GGNNCC 4 cut(s) 317, 531, 552, 1209
BspMAI CTGCAG 3 cut(s) 805, 999, 1252
BspPI GGATC 3 cut(s) 93, 215, 497
BsrBI CCGCTC 2 cut(s) 32, 420
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 2 cut(s) 676, 1048
BsrI ACTGG 3 cut(s) 643, 837, 991
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 268
BssMI GATC 4 cut(s) 98, 220, 489, 1252
BssNI GRCGYC 1 cut(s) 157
Bst2UI CCWGG 3 cut(s) 313, 908, 1212
Bst4CI ACNGT 4 cut(s) 664, 956, 970, 1233
Bst6I CTCTTC 2 cut(s) 318, 476
BstACI GRCGYC 1 cut(s) 157
BstAPI GCANNNNNTGC 1 cut(s) 917
BstAUI TGTACA 2 cut(s) 676, 1048
BstC8I GCNNGC 3 cut(s) 34, 850, 1131
BstDEI CTNAG 5 cut(s) 86, 104, 534, 778, 929
BstF5I GGATG 3 cut(s) 454, 460, 513
BstFNI CGCG 4 cut(s) 306, 331, 410, 563
BstHHI GCGC 3 cut(s) 308, 331, 906
BstKTI GATC 4 cut(s) 101, 223, 492, 1255
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 4 cut(s) 98, 220, 489, 1252
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 8 cut(s) 42, 426, 767, 791, 800, 806, 917, 1135
BstNI CCWGG 3 cut(s) 313, 908, 1212
BstNSI RCATGY 1 cut(s) 1055
BstSCI CCNGG 6 cut(s) 268, 311, 372, 906, 1210, 1218
BstSFI CTRYAG 4 cut(s) 801, 995, 1140, 1248
BstSLI GKGCMC 1 cut(s) 1272
BstUI CGCG 4 cut(s) 306, 331, 410, 563
BstV1I GCAGC 4 cut(s) 77, 157, 787, 1234
BstV2I GAAGAC 1 cut(s) 1149
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 534
BsuRI GGCC 2 cut(s) 5, 497
BtsCI GGATG 3 cut(s) 454, 460, 513
BtsI GCAGTG 2 cut(s) 40, 992
BtsIMutI CAGTG 3 cut(s) 40, 650, 992
Cac8I GCNNGC 3 cut(s) 34, 850, 1131
CfoI GCGC 3 cut(s) 308, 331, 906
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 315, 1208
CseI GACGC 2 cut(s) 165, 623
Csp6I GTAC 4 cut(s) 370, 677, 966, 1049
CviAII CATG 5 cut(s) 610, 674, 721, 734, 1052
CviQI GTAC 4 cut(s) 370, 677, 966, 1049
DdeI CTNAG 5 cut(s) 86, 104, 534, 778, 929
DpnI GATC 4 cut(s) 100, 222, 491, 1254
DpnII GATC 4 cut(s) 98, 220, 489, 1252
DraI TTTAAA 1 cut(s) 1149
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 2 cut(s) 318, 476
EarI CTCTTC 2 cut(s) 318, 476
EciI GGCGGA 1 cut(s) 450
Eco47I GGWCC 2 cut(s) 315, 1208
Eco57I CTGAAG 2 cut(s) 32, 792
Eco81I CCTNAGG 1 cut(s) 534
Eco88I CYCGRG 1 cut(s) 602
EcoRI GAATTC 1 cut(s) 924
EcoRII CCWGG 3 cut(s) 311, 906, 1210
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 5 cut(s) 613, 677, 724, 737, 1055
FalI AAGNNNNNCTT 2 cut(s) 1058, 1090
FaqI GGGAC 2 cut(s) 196, 356
FatI CATG 5 cut(s) 609, 673, 720, 733, 1051
FauI CCCGC 2 cut(s) 568, 1162
Fnu4HI GCNGC 7 cut(s) 6, 91, 146, 254, 731, 801, 1248
FokI GGATG 3 cut(s) 461, 467, 520
Fsp4HI GCNGC 7 cut(s) 6, 91, 146, 254, 731, 801, 1248
FspBI CTAG 3 cut(s) 153, 795, 962
GlaI GCGC 3 cut(s) 307, 330, 905
GluI GCNGC 7 cut(s) 6, 91, 146, 254, 731, 801, 1248
GsuI CTGGAG 1 cut(s) 1008
HaeIII GGCC 2 cut(s) 5, 497
HapII CCGG 6 cut(s) 170, 270, 362, 374, 395, 1220
HgaI GACGC 2 cut(s) 165, 623
HhaI GCGC 3 cut(s) 308, 331, 906
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 5 cut(s) 613, 677, 724, 737, 1055
Hin6I GCGC 3 cut(s) 306, 329, 904
HinP1I GCGC 3 cut(s) 306, 329, 904
HincII GTYRAC 1 cut(s) 871
HindII GTYRAC 1 cut(s) 871
HinfI GANTC 6 cut(s) 113, 130, 232, 275, 292, 1215
HpaII CCGG 6 cut(s) 170, 270, 362, 374, 395, 1220
HphI GGTGA 5 cut(s) 240, 284, 388, 964, 1081
Hpy166II GTNNAC 3 cut(s) 871, 959, 1270
Hpy188I TCNGA 5 cut(s) 112, 118, 135, 507, 781
Hpy188III TCNNGA 4 cut(s) 224, 296, 533, 602
Hpy8I GTNNAC 3 cut(s) 871, 959, 1270
HpyAV CCTTC 7 cut(s) 425, 457, 480, 577, 739, 767, 994
HpyCH4III ACNGT 4 cut(s) 664, 956, 970, 1233
HpyCH4IV ACGT 1 cut(s) 345
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 426, 767, 791, 800, 806, 917, 1135
HpyF3I CTNAG 5 cut(s) 86, 104, 534, 778, 929
HpySE526I ACGT 1 cut(s) 345
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 5 cut(s) 613, 677, 724, 737, 1055
HspAI GCGC 3 cut(s) 306, 329, 904
Kzo9I GATC 4 cut(s) 98, 220, 489, 1252
LmnI GCTCC 6 cut(s) 21, 113, 391, 437, 806, 989
Lsp1109I GCAGC 4 cut(s) 77, 157, 787, 1234
LweI GCATC 4 cut(s) 217, 682, 772, 936
MaeI CTAG 3 cut(s) 153, 795, 962
MaeII ACGT 1 cut(s) 345
MaeIII GTNAC 4 cut(s) 38, 272, 716, 970
MalI GATC 4 cut(s) 100, 222, 491, 1254
MbiI CCGCTC 2 cut(s) 32, 420
MboI GATC 4 cut(s) 98, 220, 489, 1252
MboII GAAGA 6 cut(s) 335, 410, 493, 592, 800, 1149
MfeI CAATTG 2 cut(s) 680, 1044
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 994, 1272
MluCI AATT 6 cut(s) 618, 650, 680, 843, 924, 1044
MlyI GAGTC 3 cut(s) 226, 269, 301
MmeI TCCRAC 2 cut(s) 141, 485
MseI TTAA 4 cut(s) 570, 1148, 1179, 1282
MslI CAYNNNNRTG 1 cut(s) 738
MspI CCGG 6 cut(s) 170, 270, 362, 374, 395, 1220
MspR9I CCNGG 6 cut(s) 270, 313, 374, 908, 1212, 1220
MunI CAATTG 2 cut(s) 680, 1044
MvaI CCWGG 3 cut(s) 313, 908, 1212
MvnI CGCG 4 cut(s) 306, 331, 410, 563
MwoI GCNNNNNNNGC 8 cut(s) 42, 426, 767, 791, 800, 806, 917, 1135
NciI CCSGG 3 cut(s) 270, 374, 1220
NdeII GATC 4 cut(s) 98, 220, 489, 1252
NlaIII CATG 5 cut(s) 613, 677, 724, 737, 1055
NlaIV GGNNCC 4 cut(s) 317, 531, 552, 1209
NmuCI GTSAC 3 cut(s) 38, 272, 970
NspI RCATGY 1 cut(s) 1055
PaeR7I CTCGAG 1 cut(s) 602
PciI ACATGT 1 cut(s) 1051
PfeI GAWTC 3 cut(s) 113, 130, 1215
PflFI GACNNNGTC 1 cut(s) 1011
PfoI TCCNGGA 1 cut(s) 1210
PkrI GCNGC 7 cut(s) 7, 92, 147, 255, 732, 802, 1249
PleI GAGTC 3 cut(s) 226, 269, 300
PpsI GAGTC 3 cut(s) 226, 269, 300
PscI ACATGT 1 cut(s) 1051
Psp6I CCWGG 3 cut(s) 311, 906, 1210
PspGI CCWGG 3 cut(s) 311, 906, 1210
PspN4I GGNNCC 4 cut(s) 317, 531, 552, 1209
PspPI GGNCC 2 cut(s) 315, 1208
PstI CTGCAG 3 cut(s) 805, 999, 1252
PsuI RGATCY 1 cut(s) 98
PsyI GACNNNGTC 1 cut(s) 1011
RsaI GTAC 4 cut(s) 371, 678, 967, 1050
RsaNI GTAC 4 cut(s) 370, 677, 966, 1049
RseI CAYNNNNRTG 1 cut(s) 738
SaqAI TTAA 4 cut(s) 570, 1148, 1179, 1282
SatI GCNGC 7 cut(s) 6, 91, 146, 254, 731, 801, 1248
Sau3AI GATC 4 cut(s) 98, 220, 489, 1252
Sau96I GGNCC 2 cut(s) 315, 1208
SchI GAGTC 3 cut(s) 226, 269, 301
ScrFI CCNGG 6 cut(s) 270, 313, 374, 908, 1212, 1220
SduI GDGCHC 2 cut(s) 994, 1272
SfaNI GCATC 4 cut(s) 217, 682, 772, 936
SfcI CTRYAG 4 cut(s) 801, 995, 1140, 1248
Sfr274I CTCGAG 1 cut(s) 602
SinI GGWCC 2 cut(s) 315, 1208
SlaI CTCGAG 1 cut(s) 602
SmiMI CAYNNNNRTG 1 cut(s) 738
SmlI CTYRAG 2 cut(s) 602, 1274
SmoI CTYRAG 2 cut(s) 602, 1274
Sse9I AATT 6 cut(s) 618, 650, 680, 843, 924, 1044
SspMI CTAG 3 cut(s) 153, 795, 962
StyD4I CCNGG 6 cut(s) 268, 311, 372, 906, 1210, 1218
TaaI ACNGT 4 cut(s) 664, 956, 970, 1233
TaiI ACGT 1 cut(s) 348
TaqI TCGA 4 cut(s) 174, 290, 492, 603
TasI AATT 6 cut(s) 618, 650, 680, 843, 924, 1044
TatI WGTACW 2 cut(s) 676, 1048
TauI GCSGC 3 cut(s) 8, 256, 733
TfiI GAWTC 3 cut(s) 113, 130, 1215
Tru1I TTAA 4 cut(s) 570, 1148, 1179, 1282
Tru9I TTAA 4 cut(s) 570, 1148, 1179, 1282
TscAI CASTG 3 cut(s) 47, 650, 999
TseFI GTSAC 3 cut(s) 38, 272, 970
TseI GCWGC 4 cut(s) 90, 145, 800, 1247
Tsp45I GTSAC 3 cut(s) 38, 272, 970
TspDTI ATGAA 4 cut(s) 174, 325, 672, 906
TspRI CASTG 3 cut(s) 47, 650, 999
Tth111I GACNNNGTC 1 cut(s) 1011
VneI GTGCAC 1 cut(s) 1268
VpaK11BI GGWCC 2 cut(s) 315, 1208
XapI RAATTY 2 cut(s) 650, 924
XceI RCATGY 1 cut(s) 1055
XhoI CTCGAG 1 cut(s) 602
XspI CTAG 3 cut(s) 153, 795, 962
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.