Rh2AG342900

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0010038
Physical Location & Seq
Forward (+)
50190107 .. 50195155
5049 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG342900.1

Sequence Viewer

Length: 909 bp
ATGGCAGCCCTGAAGCTCCTCTTCTCCTCGGCTCGCCGTCACTGCTCCCATCTCTACCCGTCTCCATTTCTAAGGTCCCTCACCTCCCAATCAGATCAAATTCCCTCTCCTCCCGAACCCCTTAAACCCCAACCCGTCTCCTACGCTCCCAAACCCAAACAACACTCCTCCTCACCCGACCCGGACCCGGACCCGGCTCCGCAATCAGCACAAACACAACCGCCGCCACCGTCGCACGCGCCTCCTCTCGACTCTCCCGACTCGCCGTGGACCCGAGAAGACGCCCGGTTCGTGAAGGACGGGCCTTCCATAACCCCGGTGTCGTACCCGGCCCGAGTGGCTCCGTTGCCGGAAGATAAGGTCTCGGAGGAAGGCGGCGACGCGAGGCAGGGTGAGCTGGAGAAGGAGAAGCAGAGGATCGAGCAGTCGAGAAGGAGGGTTTTTAGGGTAGAGGAGAATGTGGTGGTTCCTTTCCCCACGTTTCTTAAGGTGGAGAAGAAGGAAAGGAAGCCCATTCTTGATTTGATGGAAGCAATTCGACAAGTCAAGGCTACTGCCAAGTGCAACTTTGATGAAACTGTTGAAGCTCACGTGCAATTGGGTATTGATGCCAAGCGAAATGCAGTTCGTGGCAACATGACCTTGCCCCATGGTAGCGGAAAGCTAGGTACTGTGACTAACGACATTGCTGGGGCACTACAAAAAGTAAGACAAGGTCATATTGAGTATAAAATGGACAGAACATCAATAGTTCATGTTGGGCTCGGAAAGGTAAACTTTACAGAGGAGTATCTGCGTGAGAATGTTGGTGCATTTATGAGTTCTCTTTTGCTGGCAAAGCCTACGGGTTTAAAAAAGTCTTCCAAATATGCTGGGTATATCAACTCTTTCCACATATGTAGCACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

302

Amino Acids

33.41

Weight (kDa)

9.2

Isoelectric Point (pI)

67.78

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 222 - 302 4.5e-14 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 239, 383
AciI CCGC 5 cut(s) 200, 221, 224, 375, 657
AclWI GGATC 1 cut(s) 425
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 1 cut(s) 32
AcvI CACGTG 1 cut(s) 592
AcyI GRCGYC 1 cut(s) 282
AfaI GTAC 2 cut(s) 326, 670
AfiI CCNNNNNNNGG 2 cut(s) 187, 193
AflII CTTAAG 1 cut(s) 485
AgsI TTSAA 1 cut(s) 584
AluBI AGCT 4 cut(s) 16, 397, 587, 664
AluI AGCT 4 cut(s) 16, 397, 587, 664
Alw26I GTCTC 3 cut(s) 66, 142, 367
AlwI GGATC 1 cut(s) 425
Ama87I CYCGRG 2 cut(s) 273, 333
AoxI GGCC 2 cut(s) 302, 330
ApeKI GCWGC 1 cut(s) 5
ApoI RAATTY 1 cut(s) 99
ArsI GACNNNNNNTTYG 2 cut(s) 272, 304
Asp700I GAANNNNTTC 1 cut(s) 534
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 6 cut(s) 75, 184, 190, 270, 302, 331
AsuC2I CCSGG 6 cut(s) 182, 188, 194, 286, 317, 329
AsuHPI GGTGA 3 cut(s) 73, 165, 404
AvaI CYCGRG 2 cut(s) 273, 333
AvaII GGWCC 4 cut(s) 75, 184, 190, 270
BaeGI GKGCMC 1 cut(s) 697
BanII GRGCYC 1 cut(s) 765
BbrPI CACGTG 1 cut(s) 592
BbsI GAAGAC 2 cut(s) 285, 852
BbvI GCAGC 1 cut(s) 17
BccI CCATC 2 cut(s) 57, 520
BceAI ACGGC 2 cut(s) 21, 250
BcnI CCSGG 6 cut(s) 182, 188, 194, 286, 317, 329
BcoDI GTCTC 3 cut(s) 66, 142, 367
BfaI CTAG 1 cut(s) 665
BfrI CTTAAG 1 cut(s) 485
BglI GCCNNNNNGGC 1 cut(s) 338
BisI GCNGC 3 cut(s) 6, 224, 376
BlsI GCNGC 3 cut(s) 7, 225, 377
Bme1390I CCNGG 6 cut(s) 182, 188, 194, 286, 317, 329
Bme18I GGWCC 4 cut(s) 75, 184, 190, 270
BmeT110I CYCGRG 2 cut(s) 273, 333
BmgT120I GGNCC 6 cut(s) 75, 184, 190, 270, 302, 331
BmiI GGNNCC 7 cut(s) 77, 186, 192, 198, 272, 342, 468
BmrFI CCNGG 6 cut(s) 182, 188, 194, 286, 317, 329
BmsI GCATC 1 cut(s) 598
BpiI GAAGAC 2 cut(s) 285, 852
BpmI CTGGAG 1 cut(s) 419
BpuMI CCSGG 6 cut(s) 182, 188, 194, 286, 317, 329
BsaAI YACGTR 1 cut(s) 592
BsaHI GRCGYC 1 cut(s) 282
BsaI GGTCTC 1 cut(s) 367
BsaJI CCNNGG 4 cut(s) 27, 266, 315, 649
Bsc4I CCNNNNNNNGG 2 cut(s) 187, 193
Bse3DI GCAATG 1 cut(s) 684
BseDI CCNNGG 4 cut(s) 27, 266, 315, 649
BseLI CCNNNNNNNGG 2 cut(s) 187, 193
BseMI GCAATG 1 cut(s) 684
BseRI GAGGAG 8 cut(s) 8, 16, 99, 157, 160, 234, 467, 800
BseSI GKGCMC 1 cut(s) 697
BseXI GCAGC 1 cut(s) 17
BseYI CCCAGC 2 cut(s) 689, 872
Bsh1236I CGCG 2 cut(s) 239, 383
BshFI GGCC 2 cut(s) 304, 332
BsiHKCI CYCGRG 2 cut(s) 273, 333
BsiSI CCGG 7 cut(s) 182, 188, 194, 286, 317, 329, 350
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 2 cut(s) 187, 193
BsmAI GTCTC 3 cut(s) 66, 142, 367
BsmBI CGTCTC 2 cut(s) 66, 142
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 304, 332
Bso31I GGTCTC 1 cut(s) 367
BsoBI CYCGRG 2 cut(s) 273, 333
Bsp1286I GDGCHC 2 cut(s) 697, 765
Bsp143I GATC 2 cut(s) 94, 417
Bsp19I CCATGG 1 cut(s) 649
BspACI CCGC 5 cut(s) 200, 221, 224, 375, 657
BspANI GGCC 2 cut(s) 304, 332
BspFNI CGCG 2 cut(s) 239, 383
BspLI GGNNCC 7 cut(s) 77, 186, 192, 198, 272, 342, 468
BspPI GGATC 1 cut(s) 425
BspTI CTTAAG 1 cut(s) 485
BspTNI GGTCTC 1 cut(s) 367
BsrDI GCAATG 1 cut(s) 684
BssECI CCNNGG 4 cut(s) 27, 266, 315, 649
BssMI GATC 2 cut(s) 94, 417
BssNI GRCGYC 1 cut(s) 282
BssT1I CCWWGG 1 cut(s) 649
Bst4CI ACNGT 3 cut(s) 231, 580, 673
Bst6I CTCTTC 1 cut(s) 26
BstACI GRCGYC 1 cut(s) 282
BstAFI CTTAAG 1 cut(s) 485
BstBAI YACGTR 1 cut(s) 592
BstC8I GCNNGC 3 cut(s) 34, 237, 834
BstDEI CTNAG 1 cut(s) 71
BstDSI CCRYGG 2 cut(s) 266, 649
BstFNI CGCG 2 cut(s) 239, 383
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 2 cut(s) 97, 420
BstMAI GTCTC 3 cut(s) 66, 142, 367
BstMBI GATC 2 cut(s) 94, 417
BstMWI GCNNNNNNNGC 5 cut(s) 42, 232, 338, 394, 838
BstSCI CCNGG 6 cut(s) 180, 186, 192, 284, 315, 327
BstSLI GKGCMC 1 cut(s) 697
BstUI CGCG 2 cut(s) 239, 383
BstV1I GCAGC 1 cut(s) 17
BstV2I GAAGAC 2 cut(s) 285, 852
BsuRI GGCC 2 cut(s) 304, 332
BtgI CCRYGG 2 cut(s) 266, 649
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 3 cut(s) 34, 237, 834
CfoI GCGC 1 cut(s) 241
Cfr13I GGNCC 6 cut(s) 75, 184, 190, 270, 302, 331
CseI GACGC 2 cut(s) 290, 389
Csp6I GTAC 2 cut(s) 325, 669
CviAII CATG 4 cut(s) 637, 650, 755, 906
CviQI GTAC 2 cut(s) 325, 669
DdeI CTNAG 1 cut(s) 71
DpnI GATC 2 cut(s) 96, 419
DpnII GATC 2 cut(s) 94, 417
DraI TTTAAA 1 cut(s) 852
Eam1104I CTCTTC 1 cut(s) 26
EarI CTCTTC 1 cut(s) 26
Eco130I CCWWGG 1 cut(s) 649
Eco24I GRGCYC 1 cut(s) 765
Eco31I GGTCTC 1 cut(s) 367
Eco47I GGWCC 4 cut(s) 75, 184, 190, 270
Eco57I CTGAAG 1 cut(s) 32
Eco72I CACGTG 1 cut(s) 592
Eco88I CYCGRG 2 cut(s) 273, 333
EcoO109I RGGNCCY 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 649
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 1 cut(s) 649
Esp3I CGTCTC 2 cut(s) 66, 142
FaeI CATG 4 cut(s) 640, 653, 758, 909
FalI AAGNNNNNCTT 5 cut(s) 37, 551, 583, 761, 793
FaqI GGGAC 1 cut(s) 61
FatI CATG 4 cut(s) 636, 649, 754, 905
FauNDI CATATG 1 cut(s) 896
Fnu4HI GCNGC 3 cut(s) 6, 224, 376
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 3 cut(s) 6, 224, 376
FspBI CTAG 1 cut(s) 665
GlaI GCGC 1 cut(s) 240
GluI GCNGC 3 cut(s) 6, 224, 376
GsaI CCCAGC 2 cut(s) 693, 876
GsuI CTGGAG 1 cut(s) 419
HaeIII GGCC 2 cut(s) 304, 332
HapII CCGG 7 cut(s) 182, 188, 194, 286, 317, 329, 350
HgaI GACGC 2 cut(s) 290, 389
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 282
Hin1II CATG 4 cut(s) 640, 653, 758, 909
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HinfI GANTC 2 cut(s) 251, 260
HpaII CCGG 7 cut(s) 182, 188, 194, 286, 317, 329, 350
HphI GGTGA 3 cut(s) 73, 165, 404
Hpy166II GTNNAC 2 cut(s) 270, 775
Hpy188I TCNGA 3 cut(s) 94, 367, 767
Hpy188III TCNNGA 6 cut(s) 113, 248, 257, 292, 429, 518
Hpy8I GTNNAC 2 cut(s) 270, 775
Hpy99I CGWCG 2 cut(s) 235, 383
HpyAV CCTTC 6 cut(s) 289, 315, 365, 397, 426, 493
HpyCH4III ACNGT 3 cut(s) 231, 580, 673
HpyCH4IV ACGT 2 cut(s) 479, 591
HpyCH4V TGCA 4 cut(s) 564, 595, 623, 812
HpyF10VI GCNNNNNNNGC 5 cut(s) 42, 232, 338, 394, 838
HpyF3I CTNAG 1 cut(s) 71
HpySE526I ACGT 2 cut(s) 479, 591
Hsp92I GRCGYC 1 cut(s) 282
Hsp92II CATG 4 cut(s) 640, 653, 758, 909
HspAI GCGC 1 cut(s) 239
Kzo9I GATC 2 cut(s) 94, 417
LmnI GCTCC 5 cut(s) 21, 50, 151, 202, 346
Lsp1109I GCAGC 1 cut(s) 17
LweI GCATC 1 cut(s) 598
MaeI CTAG 1 cut(s) 665
MaeII ACGT 2 cut(s) 479, 591
MaeIII GTNAC 2 cut(s) 38, 673
MalI GATC 2 cut(s) 96, 419
MboI GATC 2 cut(s) 94, 417
MboII GAAGA 5 cut(s) 13, 290, 365, 508, 852
MfeI CAATTG 1 cut(s) 596
MhlI GDGCHC 2 cut(s) 697, 765
MluCI AATT 3 cut(s) 99, 534, 596
MlyI GAGTC 2 cut(s) 245, 254
MroXI GAANNNNTTC 1 cut(s) 534
MseI TTAA 3 cut(s) 123, 486, 851
MspCI CTTAAG 1 cut(s) 485
MspI CCGG 7 cut(s) 182, 188, 194, 286, 317, 329, 350
MspR9I CCNGG 6 cut(s) 182, 188, 194, 286, 317, 329
MunI CAATTG 1 cut(s) 596
MvnI CGCG 2 cut(s) 239, 383
MwoI GCNNNNNNNGC 5 cut(s) 42, 232, 338, 394, 838
NciI CCSGG 6 cut(s) 182, 188, 194, 286, 317, 329
NcoI CCATGG 1 cut(s) 649
NdeI CATATG 1 cut(s) 896
NdeII GATC 2 cut(s) 94, 417
NlaIII CATG 4 cut(s) 640, 653, 758, 909
NlaIV GGNNCC 7 cut(s) 77, 186, 192, 198, 272, 342, 468
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 38, 673
PcsI WCGNNNNNNNCGW 2 cut(s) 255, 288
PdmI GAANNNNTTC 1 cut(s) 534
PflFI GACNNNGTC 1 cut(s) 714
PkrI GCNGC 3 cut(s) 7, 225, 377
PleI GAGTC 2 cut(s) 245, 254
PmaCI CACGTG 1 cut(s) 592
PmlI CACGTG 1 cut(s) 592
PpsI GAGTC 2 cut(s) 245, 254
Ppu21I YACGTR 1 cut(s) 592
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
PspCI CACGTG 1 cut(s) 592
PspFI CCCAGC 2 cut(s) 689, 872
PspN4I GGNNCC 7 cut(s) 77, 186, 192, 198, 272, 342, 468
PspPI GGNCC 6 cut(s) 75, 184, 190, 270, 302, 331
PspPPI RGGWCCY 1 cut(s) 75
PsyI GACNNNGTC 1 cut(s) 714
RsaI GTAC 2 cut(s) 326, 670
RsaNI GTAC 2 cut(s) 325, 669
SaqAI TTAA 3 cut(s) 123, 486, 851
SatI GCNGC 3 cut(s) 6, 224, 376
Sau3AI GATC 2 cut(s) 94, 417
Sau96I GGNCC 6 cut(s) 75, 184, 190, 270, 302, 331
SchI GAGTC 2 cut(s) 245, 254
ScrFI CCNGG 6 cut(s) 182, 188, 194, 286, 317, 329
SduI GDGCHC 2 cut(s) 697, 765
SfaNI GCATC 1 cut(s) 598
SinI GGWCC 4 cut(s) 75, 184, 190, 270
SmlI CTYRAG 1 cut(s) 485
SmoI CTYRAG 1 cut(s) 485
Sse9I AATT 3 cut(s) 99, 534, 596
SsiI CCGC 5 cut(s) 200, 221, 224, 375, 657
SspMI CTAG 1 cut(s) 665
StyD4I CCNGG 6 cut(s) 180, 186, 192, 284, 315, 327
StyI CCWWGG 1 cut(s) 649
TaaI ACNGT 3 cut(s) 231, 580, 673
TaiI ACGT 2 cut(s) 482, 594
TaqI TCGA 4 cut(s) 249, 420, 428, 538
TasI AATT 3 cut(s) 99, 534, 596
TauI GCSGC 2 cut(s) 226, 378
Tru1I TTAA 3 cut(s) 123, 486, 851
Tru9I TTAA 3 cut(s) 123, 486, 851
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 38, 673
TseI GCWGC 1 cut(s) 5
Tsp45I GTSAC 2 cut(s) 38, 673
TspDTI ATGAA 2 cut(s) 588, 743
TspGWI ACGGA 1 cut(s) 333
TspRI CASTG 1 cut(s) 47
Tth111I GACNNNGTC 1 cut(s) 714
Vha464I CTTAAG 1 cut(s) 485
VpaK11BI GGWCC 4 cut(s) 75, 184, 190, 270
XapI RAATTY 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 534
XspI CTAG 1 cut(s) 665
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.