MD17G1265500.v1.1

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0010038
Physical Location & Seq
Reverse (-)
32599867 .. 32604342
4476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1265500.v1.1.491

Sequence Viewer

Length: 1290 bp
ATGGCCGCCCTGAAGCTCTTCCTCTCATCCGCTCGCCGTCACTGCCTCTTCCAACCTCACTCCCGTCTCCACCCGCCTCCATTTCTAAGCTATCACAGATCCCTAAGCTCCGAATCCGACCCAAACCCGGATCAGAACCCTCCGCAACCTCCTAGACGCCAACCAGTGCCGGTCGAACCCGTTTCATACACTCTCAAACCCAAGGAGCAATCCCTACCTGAACCAGATGCGACTCCCCAAGCAGCACAAACATCACCGCCGCCGCCACAAAGCTCCAGTGACTCGCTCAACCCATCGAGTCAGGACACTCGCGTCACCTGGACCCGTGAAGAGGCGCGTTTCATCAGGGACGTGCCATCTATATCTCCGGTATCGTACCCGGTGAAAGTTGCTCCGTTACCGGAAGATCGGGTCGCGGAGGAGAGCGGAGGTGGCGAAGACGGAGCTGGAAGGGATGTGGATGGCGTGAAGGAGTTGGACAGAGAGAAGCGGAGGATCGAGGCCGATGGTCGGATGAGAAGGAGGCTTTTTAGGGTTCCTGAGGAGGAGGTGGCAGTTCCTTTTCCCGCGTTGATTAAGGTTGAGAAGAAGGAGAGCAAGCCCATTCTCGATGTCATGGACGCAATTCGCCAAGTCAAGGCTAATGCCAAGTGCAATTTTGATGAAACTGTTGAAGCTCATGTACAATTGGGTATTGATGCGAAGCGAAATGCAGTTCGTGGTAACATGACTTTGCCGCATGGTAGTGGGAAGGTTGTCAGGGTTGCTTTCTTTGCTGAAGGCTCAGATGCAGAAGAAGCTAGAGCTGCAGGAGCAGATATTGTTGGCGGTGTTGAACTGGTGGAGGAAATTGCAAGCAGTCATAAGCTCAATGTTGACAAGTGTTTTGCAACAAATGAAATGATTATGCGTCTGGCAAAGATTGCAAGAATTCTTAGAGAGCGTGGTTTGATGCCTGACCGTAAACTAGGTACTGTCACCAATGACATTACTGGAGCACTACAGAAAGTAAGACAAGGTCATATTGAGTATAGAATGGACAGAACATCAATTGTACATGTGGGACTTGGAAAGGTGAGCTTTACAGAGGATTATCTCCGTGAGAACATCGGAGCATTTATGAGTTCTCTTTTGCTGGCAAAGCCTACGGGTTTGAAAAAGTCTTCCAAATATGCGGGGTATATTAACTCCTTCCATATATGTAGCACGATGGGTCTAGGATTTCCGGTTTCAATACAGTCGTTATCCAAAGCTGCAGATCACTACAACAAAGTGCACCTTAAGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

430

Amino Acids

47.36

Weight (kDa)

8.98

Isoelectric Point (pI)

58.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 212 - 405 4.3e-46 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 311
AccBSI CCGCTC 2 cut(s) 32, 426
AccII CGCG 4 cut(s) 312, 337, 416, 569
AclWI GGATC 3 cut(s) 93, 138, 503
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 930
AcuI CTGAAG 2 cut(s) 32, 798
AcyI GRCGYC 1 cut(s) 157
AfaI GTAC 4 cut(s) 377, 684, 973, 1056
AfiI CCNNNNNNNGG 4 cut(s) 127, 331, 510, 637
AflII CTTAAG 1 cut(s) 1280
AflIII ACRYGT 1 cut(s) 1057
AgsI TTSAA 4 cut(s) 674, 836, 1156, 1233
AjiI CACGTC 1 cut(s) 352
AjnI CCWGG 1 cut(s) 317
Alw21I GWGCWC 2 cut(s) 1000, 1278
Alw26I GTCTC 1 cut(s) 71
Alw44I GTGCAC 1 cut(s) 1274
AlwI GGATC 3 cut(s) 93, 138, 503
AoxI GGCC 2 cut(s) 3, 501
ApaLI GTGCAC 1 cut(s) 1274
ApeKI GCWGC 3 cut(s) 242, 806, 1253
ApoI RAATTY 1 cut(s) 930
ArsI GACNNNNNNTTYG 2 cut(s) 869, 901
Asp700I GAANNNNTTC 1 cut(s) 17
AspLEI GCGC 1 cut(s) 337
AspS9I GGNCC 1 cut(s) 321
AsuC2I CCSGG 2 cut(s) 128, 380
AsuHPI GGTGA 5 cut(s) 246, 307, 394, 970, 1087
AvaII GGWCC 1 cut(s) 321
AxyI CCTNAGG 1 cut(s) 540
BaeGI GKGCMC 1 cut(s) 1278
BarI GAAGNNNNNNTAC 2 cut(s) 666, 698
BbsI GAAGAC 2 cut(s) 444, 1155
Bbv12I GWGCWC 2 cut(s) 1000, 1278
BbvI GCAGC 3 cut(s) 254, 793, 1240
BccI CCATC 5 cut(s) 301, 364, 455, 500, 1204
BceAI ACGGC 1 cut(s) 21
BciT130I CCWGG 1 cut(s) 319
BcnI CCSGG 2 cut(s) 128, 380
BcoDI GTCTC 1 cut(s) 71
BfaI CTAG 4 cut(s) 153, 801, 968, 1217
BfmI CTRYAG 3 cut(s) 807, 1001, 1254
BfrI CTTAAG 1 cut(s) 1280
BisI GCNGC 7 cut(s) 6, 243, 260, 263, 737, 807, 1254
BlsI GCNGC 7 cut(s) 7, 244, 261, 264, 738, 808, 1255
Bme1390I CCNGG 3 cut(s) 128, 319, 380
Bme18I GGWCC 1 cut(s) 321
BmgBI CACGTC 1 cut(s) 352
BmgT120I GGNCC 1 cut(s) 321
BmiI GGNNCC 2 cut(s) 323, 537
BmrFI CCNGG 3 cut(s) 128, 319, 380
BmsI GCATC 4 cut(s) 217, 688, 778, 942
BpiI GAAGAC 2 cut(s) 444, 1155
BpmI CTGGAG 2 cut(s) 259, 1014
Bpu10I CCTNAGC 1 cut(s) 104
BpuMI CCSGG 2 cut(s) 128, 380
BsaHI GRCGYC 1 cut(s) 157
BsaJI CCNNGG 1 cut(s) 201
BsaWI WCCGGW 3 cut(s) 367, 400, 1225
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 331, 510, 637
Bse118I RCCGGY 1 cut(s) 169
Bse1I ACTGG 4 cut(s) 164, 276, 843, 997
Bse21I CCTNAGG 1 cut(s) 540
BseBI CCWGG 1 cut(s) 319
BseDI CCNNGG 1 cut(s) 201
BseGI GGATG 4 cut(s) 26, 460, 466, 519
BseLI CCNNNNNNNGG 4 cut(s) 127, 331, 510, 637
BseMII CTCAG 2 cut(s) 531, 798
BseNI ACTGG 4 cut(s) 164, 276, 843, 997
BseRI GAGGAG 3 cut(s) 434, 557, 560
BseSI GKGCMC 1 cut(s) 1278
BseXI GCAGC 3 cut(s) 254, 793, 1240
Bsh1236I CGCG 4 cut(s) 312, 337, 416, 569
Bsh1285I CGRYCG 1 cut(s) 174
BshFI GGCC 2 cut(s) 5, 503
BsiEI CGRYCG 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 1000, 1278
BsiSI CCGG 6 cut(s) 128, 170, 368, 380, 401, 1226
BslFI GGGAC 2 cut(s) 362, 1077
BslI CCNNNNNNNGG 4 cut(s) 127, 331, 510, 637
BsmAI GTCTC 1 cut(s) 71
BsmBI CGTCTC 1 cut(s) 71
BsmFI GGGAC 2 cut(s) 362, 1077
BsnI GGCC 2 cut(s) 5, 503
Bsp1286I GDGCHC 2 cut(s) 1000, 1278
Bsp1407I TGTACA 2 cut(s) 682, 1054
Bsp143I GATC 5 cut(s) 98, 130, 406, 495, 1258
BspANI GGCC 2 cut(s) 5, 503
BspCNI CTCAG 2 cut(s) 532, 797
BspFNI CGCG 4 cut(s) 312, 337, 416, 569
BspLI GGNNCC 2 cut(s) 323, 537
BspMAI CTGCAG 2 cut(s) 811, 1258
BspPI GGATC 3 cut(s) 93, 138, 503
BspQI GCTCTTC 1 cut(s) 23
BspTI CTTAAG 1 cut(s) 1280
BsrBI CCGCTC 2 cut(s) 32, 426
BsrFI RCCGGY 1 cut(s) 169
BsrGI TGTACA 2 cut(s) 682, 1054
BsrI ACTGG 4 cut(s) 164, 276, 843, 997
BssAI RCCGGY 1 cut(s) 169
BssECI CCNNGG 1 cut(s) 201
BssMI GATC 5 cut(s) 98, 130, 406, 495, 1258
BssNI GRCGYC 1 cut(s) 157
BssT1I CCWWGG 1 cut(s) 201
Bst2UI CCWGG 1 cut(s) 319
Bst4CI ACNGT 4 cut(s) 670, 962, 976, 1239
Bst6I CTCTTC 3 cut(s) 23, 53, 324
BstACI GRCGYC 1 cut(s) 157
BstAFI CTTAAG 1 cut(s) 1280
BstAPI GCANNNNNTGC 1 cut(s) 923
BstAUI TGTACA 2 cut(s) 682, 1054
BstC8I GCNNGC 4 cut(s) 34, 599, 856, 1137
BstDEI CTNAG 5 cut(s) 86, 104, 540, 784, 935
BstF5I GGATG 4 cut(s) 26, 460, 466, 519
BstFNI CGCG 4 cut(s) 312, 337, 416, 569
BstHHI GCGC 1 cut(s) 337
BstKTI GATC 5 cut(s) 101, 133, 409, 498, 1261
BstMAI GTCTC 1 cut(s) 71
BstMBI GATC 5 cut(s) 98, 130, 406, 495, 1258
BstMCI CGRYCG 1 cut(s) 174
BstMWI GCNNNNNNNGC 8 cut(s) 42, 432, 773, 797, 806, 812, 923, 1141
BstNI CCWGG 1 cut(s) 319
BstNSI RCATGY 1 cut(s) 1061
BstSCI CCNGG 3 cut(s) 126, 317, 378
BstSFI CTRYAG 3 cut(s) 807, 1001, 1254
BstSLI GKGCMC 1 cut(s) 1278
BstUI CGCG 4 cut(s) 312, 337, 416, 569
BstV1I GCAGC 3 cut(s) 254, 793, 1240
BstV2I GAAGAC 2 cut(s) 444, 1155
BstX2I RGATCY 1 cut(s) 98
BstYI RGATCY 1 cut(s) 98
Bsu36I CCTNAGG 1 cut(s) 540
BsuRI GGCC 2 cut(s) 5, 503
BtrI CACGTC 1 cut(s) 352
BtsCI GGATG 4 cut(s) 26, 460, 466, 519
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 3 cut(s) 40, 171, 283
Cac8I GCNNGC 4 cut(s) 34, 599, 856, 1137
CfoI GCGC 1 cut(s) 337
Cfr10I RCCGGY 1 cut(s) 169
Cfr13I GGNCC 1 cut(s) 321
CseI GACGC 4 cut(s) 165, 301, 629, 899
Csp6I GTAC 4 cut(s) 376, 683, 972, 1055
CviAII CATG 5 cut(s) 616, 680, 727, 740, 1058
CviQI GTAC 4 cut(s) 376, 683, 972, 1055
DdeI CTNAG 5 cut(s) 86, 104, 540, 784, 935
DpnI GATC 5 cut(s) 100, 132, 408, 497, 1260
DpnII GATC 5 cut(s) 98, 130, 406, 495, 1258
DrdI GACNNNNNNGTC 1 cut(s) 311
DseDI GACNNNNNNGTC 1 cut(s) 311
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 23, 53, 324
EarI CTCTTC 3 cut(s) 23, 53, 324
Eco130I CCWWGG 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 321
Eco57I CTGAAG 2 cut(s) 32, 798
Eco81I CCTNAGG 1 cut(s) 540
EcoRI GAATTC 1 cut(s) 930
EcoRII CCWGG 1 cut(s) 317
EcoT14I CCWWGG 1 cut(s) 201
ErhI CCWWGG 1 cut(s) 201
Esp3I CGTCTC 1 cut(s) 71
FaeI CATG 5 cut(s) 619, 683, 730, 743, 1061
FalI AAGNNNNNCTT 3 cut(s) 1064, 1096, 1263
FaqI GGGAC 2 cut(s) 362, 1077
FatI CATG 5 cut(s) 615, 679, 726, 739, 1057
FauI CCCGC 3 cut(s) 81, 574, 1168
Fnu4HI GCNGC 7 cut(s) 6, 243, 260, 263, 737, 807, 1254
FokI GGATG 4 cut(s) 13, 467, 473, 526
Fsp4HI GCNGC 7 cut(s) 6, 243, 260, 263, 737, 807, 1254
FspBI CTAG 4 cut(s) 153, 801, 968, 1217
GlaI GCGC 1 cut(s) 336
GluI GCNGC 7 cut(s) 6, 243, 260, 263, 737, 807, 1254
GsuI CTGGAG 2 cut(s) 259, 1014
HaeIII GGCC 2 cut(s) 5, 503
HapII CCGG 6 cut(s) 128, 170, 368, 380, 401, 1226
HgaI GACGC 4 cut(s) 165, 301, 629, 899
HhaI GCGC 1 cut(s) 337
Hin1I GRCGYC 1 cut(s) 157
Hin1II CATG 5 cut(s) 619, 683, 730, 743, 1061
Hin6I GCGC 1 cut(s) 335
HinP1I GCGC 1 cut(s) 335
HincII GTYRAC 1 cut(s) 877
HindII GTYRAC 1 cut(s) 877
HinfI GANTC 4 cut(s) 113, 232, 281, 298
HpaII CCGG 6 cut(s) 128, 170, 368, 380, 401, 1226
HphI GGTGA 5 cut(s) 246, 307, 394, 970, 1087
Hpy166II GTNNAC 3 cut(s) 877, 965, 1276
Hpy188I TCNGA 6 cut(s) 112, 118, 135, 513, 787, 1112
Hpy188III TCNNGA 3 cut(s) 302, 539, 608
Hpy8I GTNNAC 3 cut(s) 877, 965, 1276
HpyAV CCTTC 7 cut(s) 444, 463, 513, 583, 745, 773, 1201
HpyCH4III ACNGT 4 cut(s) 670, 962, 976, 1239
HpyCH4IV ACGT 1 cut(s) 351
HpyCH4V TGCA 9 cut(s) 654, 713, 791, 809, 854, 890, 926, 1256, 1276
HpyF10VI GCNNNNNNNGC 8 cut(s) 42, 432, 773, 797, 806, 812, 923, 1141
HpyF3I CTNAG 5 cut(s) 86, 104, 540, 784, 935
HpySE526I ACGT 1 cut(s) 351
Hsp92I GRCGYC 1 cut(s) 157
Hsp92II CATG 5 cut(s) 619, 683, 730, 743, 1061
HspAI GCGC 1 cut(s) 335
Kzo9I GATC 5 cut(s) 98, 130, 406, 495, 1258
LguI GCTCTTC 1 cut(s) 23
LmnI GCTCC 8 cut(s) 113, 205, 278, 397, 443, 812, 995, 1112
Lsp1109I GCAGC 3 cut(s) 254, 793, 1240
LweI GCATC 4 cut(s) 217, 688, 778, 942
MaeI CTAG 4 cut(s) 153, 801, 968, 1217
MaeII ACGT 1 cut(s) 351
MaeIII GTNAC 6 cut(s) 38, 278, 313, 396, 722, 976
MalI GATC 5 cut(s) 100, 132, 408, 497, 1260
MbiI CCGCTC 2 cut(s) 32, 426
MboI GATC 5 cut(s) 98, 130, 406, 495, 1258
MboII GAAGA 8 cut(s) 10, 40, 341, 416, 449, 598, 806, 1155
MfeI CAATTG 2 cut(s) 686, 1050
MflI RGATCY 1 cut(s) 98
MhlI GDGCHC 2 cut(s) 1000, 1278
MluCI AATT 6 cut(s) 624, 655, 686, 849, 930, 1050
MlyI GAGTC 3 cut(s) 226, 275, 307
MmeI TCCRAC 4 cut(s) 76, 141, 456, 491
MroXI GAANNNNTTC 1 cut(s) 17
MseI TTAA 4 cut(s) 576, 1185, 1281, 1288
MslI CAYNNNNRTG 1 cut(s) 744
MspCI CTTAAG 1 cut(s) 1280
MspI CCGG 6 cut(s) 128, 170, 368, 380, 401, 1226
MspR9I CCNGG 3 cut(s) 128, 319, 380
MunI CAATTG 2 cut(s) 686, 1050
MvaI CCWGG 1 cut(s) 319
MvnI CGCG 4 cut(s) 312, 337, 416, 569
MwoI GCNNNNNNNGC 8 cut(s) 42, 432, 773, 797, 806, 812, 923, 1141
NciI CCSGG 2 cut(s) 128, 380
NdeII GATC 5 cut(s) 98, 130, 406, 495, 1258
NlaIII CATG 5 cut(s) 619, 683, 730, 743, 1061
NlaIV GGNNCC 2 cut(s) 323, 537
NmuCI GTSAC 4 cut(s) 38, 278, 313, 976
NspI RCATGY 1 cut(s) 1061
PciI ACATGT 1 cut(s) 1057
PciSI GCTCTTC 1 cut(s) 23
PdmI GAANNNNTTC 1 cut(s) 17
PfeI GAWTC 1 cut(s) 113
PflFI GACNNNGTC 1 cut(s) 1017
PkrI GCNGC 7 cut(s) 7, 244, 261, 264, 738, 808, 1255
PleI GAGTC 3 cut(s) 226, 275, 306
PpsI GAGTC 3 cut(s) 226, 275, 306
PscI ACATGT 1 cut(s) 1057
Psp6I CCWGG 1 cut(s) 317
PspGI CCWGG 1 cut(s) 317
PspN4I GGNNCC 2 cut(s) 323, 537
PspPI GGNCC 1 cut(s) 321
PstI CTGCAG 2 cut(s) 811, 1258
PsuI RGATCY 1 cut(s) 98
PsyI GACNNNGTC 1 cut(s) 1017
RsaI GTAC 4 cut(s) 377, 684, 973, 1056
RsaNI GTAC 4 cut(s) 376, 683, 972, 1055
RseI CAYNNNNRTG 1 cut(s) 744
SapI GCTCTTC 1 cut(s) 23
SaqAI TTAA 4 cut(s) 576, 1185, 1281, 1288
SatI GCNGC 7 cut(s) 6, 243, 260, 263, 737, 807, 1254
Sau3AI GATC 5 cut(s) 98, 130, 406, 495, 1258
Sau96I GGNCC 1 cut(s) 321
SchI GAGTC 3 cut(s) 226, 275, 307
ScrFI CCNGG 3 cut(s) 128, 319, 380
SduI GDGCHC 2 cut(s) 1000, 1278
SfaNI GCATC 4 cut(s) 217, 688, 778, 942
SfcI CTRYAG 3 cut(s) 807, 1001, 1254
SinI GGWCC 1 cut(s) 321
SmiMI CAYNNNNRTG 1 cut(s) 744
SmlI CTYRAG 1 cut(s) 1280
SmoI CTYRAG 1 cut(s) 1280
Sse9I AATT 6 cut(s) 624, 655, 686, 849, 930, 1050
SspMI CTAG 4 cut(s) 153, 801, 968, 1217
StyD4I CCNGG 3 cut(s) 126, 317, 378
StyI CCWWGG 1 cut(s) 201
TaaI ACNGT 4 cut(s) 670, 962, 976, 1239
TaiI ACGT 1 cut(s) 354
TaqI TCGA 4 cut(s) 174, 296, 498, 609
TasI AATT 6 cut(s) 624, 655, 686, 849, 930, 1050
TatI WGTACW 2 cut(s) 682, 1054
TauI GCSGC 4 cut(s) 8, 262, 265, 739
TfiI GAWTC 1 cut(s) 113
Tru1I TTAA 4 cut(s) 576, 1185, 1281, 1288
Tru9I TTAA 4 cut(s) 576, 1185, 1281, 1288
TscAI CASTG 3 cut(s) 47, 171, 283
TseFI GTSAC 4 cut(s) 38, 278, 313, 976
TseI GCWGC 3 cut(s) 242, 806, 1253
Tsp45I GTSAC 4 cut(s) 38, 278, 313, 976
TspDTI ATGAA 4 cut(s) 174, 331, 678, 912
TspGWI ACGGA 3 cut(s) 384, 456, 1088
TspRI CASTG 3 cut(s) 47, 171, 283
Tth111I GACNNNGTC 1 cut(s) 1017
Vha464I CTTAAG 1 cut(s) 1280
VneI GTGCAC 1 cut(s) 1274
VpaK11BI GGWCC 1 cut(s) 321
XapI RAATTY 1 cut(s) 930
XceI RCATGY 1 cut(s) 1061
XmnI GAANNNNTTC 1 cut(s) 17
XspI CTAG 4 cut(s) 153, 801, 968, 1217
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.