Rw2G027660

50S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0010038
Physical Location & Seq
Reverse (-)
43677479 .. 43682466
4988 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G027660.1

Sequence Viewer

Length: 1203 bp
ATGGCAGCCCTGAAGCTCCTCCTCTCCTCGGCTCGCCGTCACTGCTCCCATCTCCACCCGTCTCCATTTCTAAGGTCCCTCACCTCCCAATCAGATCAAATTCCCTCTCCTCCCGAACCCCTTAAACCCCAACCCGCTCCGATCCAACCCGTCTCCTACGCTCCCAAACCCAAACAACACTCCTCCTCACCCGACCCGGACCCGGACCCGGCTCCGCAATCAGCACAAACACAACCGCCGCCACCGTCGCACGCGCCTCCTCACGACTCGCCGTGGACCCGAGAAGACGCCCGGTTCGTGAAGGACGGGCCTTCCATAACCCCGGTGTCGTACCCGGCCCGAGTGGCTCCGTTGCCGGAAGATAAGGTCTCGGAGGAAGGCGGCGACGCGAGGCAGGGTGAGCTGGAGAAGGAGAAGCAGAGGATCGAGCAGTCGAGAAGGAGGGTTTTTAGGGTAGAGGAGAATGTGGTGGTTCCTTTCCCCACGTTTCTTAAGGTGGAGAAGAAGGAAAGGAAGCCCATTCTTGATTTGATGGAAGCAATTCGACAAGTCAAGGCTACTGCCAAGTGCAACTTTGATGAAACTGTTGAAGCTCACGTGCAATTGGGTATTGATGCCAAGCGAAATGCAGTTCGTGGCAACATGACCTTGCCCCATGGTAGCGGAAAGGTAGTCAGGGTTGCTTTCTTTGCTGAAGGTGCAGATGCAGATGAAGCTAGAGCTGCTGGAGCAGATATTGTTGGCGGTGTTGACCTTGTGGAGGAAATTGCAAGCAGTCGTAAACTCAATGTTGACAAGTGTTTTGCAACAAATGAAATGGTTATGCGTCTAGCGAAGATAGCAAGGATTCTTAGAGAGCGTGGTTTAATGCCTGACCGAAAACTAGGTACTGTGACTAACGACATTGCTGGGGCACTACAAAAAGTAAGACAAGGTCATATTGAGTATAAAATGGACAGAACATCAATAGTTCATGTTGGGCTCGGAAAGGTAAGCTTTACAGAGGAGTATCTGCGTGAGAATGTTGGTGCATTTATGAGTTCTCTTTTGCTGGCAAAGCCTACGGGTTTAAAAAAGTCTTCCAAATATGCTGGGTATATCAACTCTTTCCACATATGTAGCACAATGGGCTCAGGATTTCCAGTTTCAATACAGTCATTATCCAAAGCTGCAGATAACTACAATAAAATGCACCTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_L1

Protein Analysis

400

Amino Acids

43.98

Weight (kDa)

9.31

Isoelectric Point (pI)

58.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 184 - 377 7.3e-46 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 137
AccII CGCG 2 cut(s) 254, 389
AciI CCGC 7 cut(s) 135, 215, 236, 239, 381, 663, 744
AclWI GGATC 2 cut(s) 136, 431
AcsI RAATTY 1 cut(s) 99
AcuI CTGAAG 2 cut(s) 32, 714
AcvI CACGTG 1 cut(s) 598
AcyI GRCGYC 1 cut(s) 288
AfaI GTAC 2 cut(s) 332, 889
AfiI CCNNNNNNNGG 4 cut(s) 28, 202, 208, 760
AflII CTTAAG 1 cut(s) 491
AgsI TTSAA 2 cut(s) 590, 1149
AluBI AGCT 7 cut(s) 16, 403, 593, 716, 722, 996, 1169
AluI AGCT 7 cut(s) 16, 403, 593, 716, 722, 996, 1169
Alw26I GTCTC 3 cut(s) 66, 157, 373
AlwI GGATC 2 cut(s) 136, 431
Ama87I CYCGRG 2 cut(s) 279, 339
AoxI GGCC 2 cut(s) 308, 336
ApeKI GCWGC 3 cut(s) 5, 722, 1169
ApoI RAATTY 1 cut(s) 99
ArsI GACNNNNNNTTYG 4 cut(s) 278, 310, 785, 817
Asp700I GAANNNNTTC 1 cut(s) 540
AspLEI GCGC 1 cut(s) 256
AspS9I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
AsuC2I CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
AsuHPI GGTGA 3 cut(s) 73, 180, 410
AvaI CYCGRG 2 cut(s) 279, 339
AvaII GGWCC 4 cut(s) 75, 199, 205, 276
BaeGI GKGCMC 1 cut(s) 916
BanII GRGCYC 2 cut(s) 984, 1133
BbrPI CACGTG 1 cut(s) 598
BbsI GAAGAC 2 cut(s) 291, 1071
BbvI GCAGC 3 cut(s) 17, 709, 1156
BccI CCATC 2 cut(s) 57, 526
BceAI ACGGC 2 cut(s) 21, 256
BcnI CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
BcoDI GTCTC 3 cut(s) 66, 157, 373
BfaI CTAG 3 cut(s) 717, 830, 884
BfmI CTRYAG 1 cut(s) 1170
BfrI CTTAAG 1 cut(s) 491
BglI GCCNNNNNGGC 1 cut(s) 344
BisI GCNGC 5 cut(s) 6, 239, 382, 723, 1170
BlsI GCNGC 5 cut(s) 7, 240, 383, 724, 1171
Bme1390I CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
Bme18I GGWCC 4 cut(s) 75, 199, 205, 276
BmeT110I CYCGRG 2 cut(s) 279, 339
BmgT120I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
BmiI GGNNCC 7 cut(s) 77, 201, 207, 213, 278, 348, 474
BmrFI CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
BmsI GCATC 2 cut(s) 604, 694
BpiI GAAGAC 2 cut(s) 291, 1071
BpmI CTGGAG 2 cut(s) 425, 747
Bpu10I CCTNAGC 1 cut(s) 1132
BpuEI CTTGAG 1 cut(s) 1181
BpuMI CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
BsaAI YACGTR 1 cut(s) 598
BsaHI GRCGYC 1 cut(s) 288
BsaI GGTCTC 1 cut(s) 373
BsaJI CCNNGG 4 cut(s) 27, 272, 321, 655
Bsc4I CCNNNNNNNGG 4 cut(s) 28, 202, 208, 760
Bse1I ACTGG 1 cut(s) 1142
Bse3DI GCAATG 1 cut(s) 903
BseDI CCNNGG 4 cut(s) 27, 272, 321, 655
BseLI CCNNNNNNNGG 4 cut(s) 28, 202, 208, 760
BseMI GCAATG 1 cut(s) 903
BseMII CTCAG 1 cut(s) 1146
BseNI ACTGG 1 cut(s) 1142
BseRI GAGGAG 9 cut(s) 8, 11, 16, 99, 172, 175, 249, 473, 1019
BseSI GKGCMC 1 cut(s) 916
BseXI GCAGC 3 cut(s) 17, 709, 1156
BseYI CCCAGC 2 cut(s) 908, 1091
BsgI GTGCAG 1 cut(s) 720
Bsh1236I CGCG 2 cut(s) 254, 389
BshFI GGCC 2 cut(s) 310, 338
BsiHKCI CYCGRG 2 cut(s) 279, 339
BsiSI CCGG 7 cut(s) 197, 203, 209, 292, 323, 335, 356
BslFI GGGAC 1 cut(s) 61
BslI CCNNNNNNNGG 4 cut(s) 28, 202, 208, 760
BsmAI GTCTC 3 cut(s) 66, 157, 373
BsmBI CGTCTC 2 cut(s) 66, 157
BsmFI GGGAC 1 cut(s) 61
BsnI GGCC 2 cut(s) 310, 338
Bso31I GGTCTC 1 cut(s) 373
BsoBI CYCGRG 2 cut(s) 279, 339
Bsp1286I GDGCHC 3 cut(s) 916, 984, 1133
Bsp143I GATC 3 cut(s) 94, 141, 423
Bsp19I CCATGG 1 cut(s) 655
BspACI CCGC 7 cut(s) 135, 215, 236, 239, 381, 663, 744
BspANI GGCC 2 cut(s) 310, 338
BspCNI CTCAG 1 cut(s) 1145
BspFNI CGCG 2 cut(s) 254, 389
BspLI GGNNCC 7 cut(s) 77, 201, 207, 213, 278, 348, 474
BspMAI CTGCAG 1 cut(s) 1174
BspPI GGATC 2 cut(s) 136, 431
BspTI CTTAAG 1 cut(s) 491
BspTNI GGTCTC 1 cut(s) 373
BsrBI CCGCTC 1 cut(s) 137
BsrDI GCAATG 1 cut(s) 903
BsrI ACTGG 1 cut(s) 1142
BssECI CCNNGG 4 cut(s) 27, 272, 321, 655
BssMI GATC 3 cut(s) 94, 141, 423
BssNI GRCGYC 1 cut(s) 288
BssT1I CCWWGG 1 cut(s) 655
Bst4CI ACNGT 4 cut(s) 246, 586, 892, 1155
BstACI GRCGYC 1 cut(s) 288
BstAFI CTTAAG 1 cut(s) 491
BstBAI YACGTR 1 cut(s) 598
BstC8I GCNNGC 4 cut(s) 34, 252, 772, 1053
BstDEI CTNAG 3 cut(s) 71, 851, 1132
BstDSI CCRYGG 2 cut(s) 272, 655
BstENI CCTNNNNNAGG 1 cut(s) 758
BstFNI CGCG 2 cut(s) 254, 389
BstHHI GCGC 1 cut(s) 256
BstKTI GATC 3 cut(s) 97, 144, 426
BstMAI GTCTC 3 cut(s) 66, 157, 373
BstMBI GATC 3 cut(s) 94, 141, 423
BstSCI CCNGG 6 cut(s) 195, 201, 207, 290, 321, 333
BstSFI CTRYAG 1 cut(s) 1170
BstSLI GKGCMC 1 cut(s) 916
BstUI CGCG 2 cut(s) 254, 389
BstV1I GCAGC 3 cut(s) 17, 709, 1156
BstV2I GAAGAC 2 cut(s) 291, 1071
BsuRI GGCC 2 cut(s) 310, 338
BtgI CCRYGG 2 cut(s) 272, 655
BtsI GCAGTG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 40
Cac8I GCNNGC 4 cut(s) 34, 252, 772, 1053
CfoI GCGC 1 cut(s) 256
Cfr13I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
CseI GACGC 3 cut(s) 296, 395, 815
Csp6I GTAC 2 cut(s) 331, 888
CviAII CATG 3 cut(s) 643, 656, 974
CviQI GTAC 2 cut(s) 331, 888
DdeI CTNAG 3 cut(s) 71, 851, 1132
DpnI GATC 3 cut(s) 96, 143, 425
DpnII GATC 3 cut(s) 94, 141, 423
DraI TTTAAA 1 cut(s) 1071
Eco130I CCWWGG 1 cut(s) 655
Eco24I GRGCYC 2 cut(s) 984, 1133
Eco31I GGTCTC 1 cut(s) 373
Eco47I GGWCC 4 cut(s) 75, 199, 205, 276
Eco57I CTGAAG 2 cut(s) 32, 714
Eco72I CACGTG 1 cut(s) 598
Eco88I CYCGRG 2 cut(s) 279, 339
EcoNI CCTNNNNNAGG 1 cut(s) 758
EcoO109I RGGNCCY 1 cut(s) 75
EcoT14I CCWWGG 1 cut(s) 655
EcoT38I GRGCYC 2 cut(s) 984, 1133
ErhI CCWWGG 1 cut(s) 655
Esp3I CGTCTC 2 cut(s) 66, 157
FaeI CATG 3 cut(s) 646, 659, 977
FalI AAGNNNNNCTT 4 cut(s) 557, 589, 980, 1012
FaqI GGGAC 1 cut(s) 61
FatI CATG 3 cut(s) 642, 655, 973
FauI CCCGC 1 cut(s) 142
FauNDI CATATG 1 cut(s) 1115
Fnu4HI GCNGC 5 cut(s) 6, 239, 382, 723, 1170
FriOI GRGCYC 2 cut(s) 984, 1133
Fsp4HI GCNGC 5 cut(s) 6, 239, 382, 723, 1170
FspBI CTAG 3 cut(s) 717, 830, 884
GlaI GCGC 1 cut(s) 255
GluI GCNGC 5 cut(s) 6, 239, 382, 723, 1170
GsaI CCCAGC 2 cut(s) 912, 1095
GsuI CTGGAG 2 cut(s) 425, 747
HaeIII GGCC 2 cut(s) 310, 338
HapII CCGG 7 cut(s) 197, 203, 209, 292, 323, 335, 356
HgaI GACGC 3 cut(s) 296, 395, 815
HhaI GCGC 1 cut(s) 256
Hin1I GRCGYC 1 cut(s) 288
Hin1II CATG 3 cut(s) 646, 659, 977
Hin6I GCGC 1 cut(s) 254
HinP1I GCGC 1 cut(s) 254
HincII GTYRAC 2 cut(s) 751, 793
HindII GTYRAC 2 cut(s) 751, 793
HindIII AAGCTT 1 cut(s) 994
HinfI GANTC 2 cut(s) 266, 847
HpaII CCGG 7 cut(s) 197, 203, 209, 292, 323, 335, 356
HphI GGTGA 3 cut(s) 73, 180, 410
Hpy166II GTNNAC 4 cut(s) 276, 751, 782, 793
Hpy188I TCNGA 4 cut(s) 94, 141, 373, 986
Hpy188III TCNNGA 6 cut(s) 113, 263, 298, 435, 524, 1134
Hpy8I GTNNAC 4 cut(s) 276, 751, 782, 793
Hpy99I CGWCG 2 cut(s) 250, 389
HpyAV CCTTC 7 cut(s) 295, 321, 371, 403, 432, 499, 689
HpyCH4III ACNGT 4 cut(s) 246, 586, 892, 1155
HpyCH4IV ACGT 2 cut(s) 485, 597
HpyF3I CTNAG 3 cut(s) 71, 851, 1132
HpySE526I ACGT 2 cut(s) 485, 597
Hsp92I GRCGYC 1 cut(s) 288
Hsp92II CATG 3 cut(s) 646, 659, 977
HspAI GCGC 1 cut(s) 254
Kzo9I GATC 3 cut(s) 94, 141, 423
LmnI GCTCC 7 cut(s) 21, 50, 142, 166, 217, 352, 728
Lsp1109I GCAGC 3 cut(s) 17, 709, 1156
LweI GCATC 2 cut(s) 604, 694
MaeI CTAG 3 cut(s) 717, 830, 884
MaeII ACGT 2 cut(s) 485, 597
MaeIII GTNAC 2 cut(s) 38, 892
MalI GATC 3 cut(s) 96, 143, 425
MbiI CCGCTC 1 cut(s) 137
MboI GATC 3 cut(s) 94, 141, 423
MboII GAAGA 5 cut(s) 296, 371, 514, 847, 1071
MfeI CAATTG 1 cut(s) 602
MhlI GDGCHC 3 cut(s) 916, 984, 1133
MluCI AATT 4 cut(s) 99, 540, 602, 765
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 169
MroXI GAANNNNTTC 1 cut(s) 540
MseI TTAA 4 cut(s) 123, 492, 866, 1070
MspCI CTTAAG 1 cut(s) 491
MspI CCGG 7 cut(s) 197, 203, 209, 292, 323, 335, 356
MspR9I CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
MunI CAATTG 1 cut(s) 602
MvnI CGCG 2 cut(s) 254, 389
NciI CCSGG 6 cut(s) 197, 203, 209, 292, 323, 335
NcoI CCATGG 1 cut(s) 655
NdeI CATATG 1 cut(s) 1115
NdeII GATC 3 cut(s) 94, 141, 423
NlaIII CATG 3 cut(s) 646, 659, 977
NlaIV GGNNCC 7 cut(s) 77, 201, 207, 213, 278, 348, 474
NmeAIII GCCGAG 1 cut(s) 8
NmuCI GTSAC 2 cut(s) 38, 892
PcsI WCGNNNNNNNCGW 1 cut(s) 294
PdmI GAANNNNTTC 1 cut(s) 540
PfeI GAWTC 1 cut(s) 847
PflFI GACNNNGTC 1 cut(s) 933
PkrI GCNGC 5 cut(s) 7, 240, 383, 724, 1171
PleI GAGTC 1 cut(s) 260
PmaCI CACGTG 1 cut(s) 598
PmlI CACGTG 1 cut(s) 598
PpsI GAGTC 1 cut(s) 260
Ppu21I YACGTR 1 cut(s) 598
PpuMI RGGWCCY 1 cut(s) 75
Psp5II RGGWCCY 1 cut(s) 75
PspCI CACGTG 1 cut(s) 598
PspFI CCCAGC 2 cut(s) 908, 1091
PspN4I GGNNCC 7 cut(s) 77, 201, 207, 213, 278, 348, 474
PspPI GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
PspPPI RGGWCCY 1 cut(s) 75
PstI CTGCAG 1 cut(s) 1174
PsyI GACNNNGTC 1 cut(s) 933
RsaI GTAC 2 cut(s) 332, 889
RsaNI GTAC 2 cut(s) 331, 888
SaqAI TTAA 4 cut(s) 123, 492, 866, 1070
SatI GCNGC 5 cut(s) 6, 239, 382, 723, 1170
Sau3AI GATC 3 cut(s) 94, 141, 423
Sau96I GGNCC 6 cut(s) 75, 199, 205, 276, 308, 337
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 6 cut(s) 197, 203, 209, 292, 323, 335
SduI GDGCHC 3 cut(s) 916, 984, 1133
SfaNI GCATC 2 cut(s) 604, 694
SfcI CTRYAG 1 cut(s) 1170
SinI GGWCC 4 cut(s) 75, 199, 205, 276
SmlI CTYRAG 2 cut(s) 491, 1196
SmoI CTYRAG 2 cut(s) 491, 1196
Sse9I AATT 4 cut(s) 99, 540, 602, 765
SsiI CCGC 7 cut(s) 135, 215, 236, 239, 381, 663, 744
SspMI CTAG 3 cut(s) 717, 830, 884
StyD4I CCNGG 6 cut(s) 195, 201, 207, 290, 321, 333
StyI CCWWGG 1 cut(s) 655
TaaI ACNGT 4 cut(s) 246, 586, 892, 1155
TaiI ACGT 2 cut(s) 488, 600
TaqI TCGA 3 cut(s) 426, 434, 544
TaqII GACCGA 1 cut(s) 891
TasI AATT 4 cut(s) 99, 540, 602, 765
TauI GCSGC 2 cut(s) 241, 384
TfiI GAWTC 1 cut(s) 847
Tru1I TTAA 4 cut(s) 123, 492, 866, 1070
Tru9I TTAA 4 cut(s) 123, 492, 866, 1070
TscAI CASTG 1 cut(s) 47
TseFI GTSAC 2 cut(s) 38, 892
TseI GCWGC 3 cut(s) 5, 722, 1169
Tsp45I GTSAC 2 cut(s) 38, 892
TspDTI ATGAA 4 cut(s) 594, 726, 828, 962
TspGWI ACGGA 1 cut(s) 339
TspRI CASTG 1 cut(s) 47
Tth111I GACNNNGTC 1 cut(s) 933
Vha464I CTTAAG 1 cut(s) 491
VpaK11BI GGWCC 4 cut(s) 75, 199, 205, 276
XagI CCTNNNNNAGG 1 cut(s) 758
XapI RAATTY 1 cut(s) 99
XmnI GAANNNNTTC 1 cut(s) 540
XspI CTAG 3 cut(s) 717, 830, 884
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.