FvH4_6g29870

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006184
Physical Location & Seq
Reverse (-)
23088757 .. 23089385
629 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g29870.t1

Sequence Viewer

Length: 354 bp
ATGGCTCCATCCACCAGAAACTACCCAGTAAATGGCCTCAAAATAGCAATCCTCACTGTGGCTTTCATCTTTTTCTTCTTCTTCACAATTCTACTTTCCAAATCAGTGGATTCCGAACATTCGATTCACAGTGAGAGCAGTACTGATCAGCTGCAACAAAGAAAAATGGTTCTGGGTTCAAGGCCTCCTCAGTGTGAGAACAAGTGTTTGAACTGCAGGCCTTGCTTTGCTACTTTGGTCATTCCATCTCATCACAAGACAAAGGTTTACAGTTCATCTCATAATGATGGAGATGATCGCTATTATCTACTCTCATGGAAATGCAAATGTGGACAGAAGCTATTTCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

118

Amino Acids

13.37

Weight (kDa)

9.13

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 57 - 117 1.6e-19 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 32
AfaI GTAC 1 cut(s) 142
AfiI CCNNNNNNNGG 2 cut(s) 32, 58
AgsI TTSAA 3 cut(s) 180, 211, 347
AluBI AGCT 2 cut(s) 151, 340
AluI AGCT 2 cut(s) 151, 340
AoxI GGCC 3 cut(s) 34, 182, 218
ApeKI GCWGC 1 cut(s) 151
BbvI GCAGC 1 cut(s) 138
BccI CCATC 3 cut(s) 16, 253, 281
BclI TGATCA 1 cut(s) 145
BfmI CTRYAG 1 cut(s) 214
BisI GCNGC 1 cut(s) 152
BlsI GCNGC 1 cut(s) 153
BmcAI AGTACT 1 cut(s) 142
BmiI GGNNCC 1 cut(s) 6
BmrI ACTGGG 1 cut(s) 20
BmuI ACTGGG 1 cut(s) 20
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 58
Bse1I ACTGG 1 cut(s) 26
BseGI GGATG 1 cut(s) 8
BseLI CCNNNNNNNGG 2 cut(s) 32, 58
BseMII CTCAG 1 cut(s) 203
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 177
BseXI GCAGC 1 cut(s) 138
BshFI GGCC 3 cut(s) 36, 184, 220
BslI CCNNNNNNNGG 2 cut(s) 32, 58
BsnI GGCC 3 cut(s) 36, 184, 220
Bsp143I GATC 2 cut(s) 145, 295
BspANI GGCC 3 cut(s) 36, 184, 220
BspCNI CTCAG 1 cut(s) 202
BspLI GGNNCC 1 cut(s) 6
BspMAI CTGCAG 1 cut(s) 218
BsrI ACTGG 1 cut(s) 26
BssMI GATC 2 cut(s) 145, 295
Bst4CI ACNGT 3 cut(s) 58, 131, 272
BstAPI GCANNNNNTGC 1 cut(s) 222
BstC8I GCNNGC 1 cut(s) 218
BstDEI CTNAG 1 cut(s) 189
BstF5I GGATG 1 cut(s) 8
BstKTI GATC 2 cut(s) 148, 298
BstMBI GATC 2 cut(s) 145, 295
BstMWI GCNNNNNNNGC 1 cut(s) 222
BstSFI CTRYAG 1 cut(s) 214
BstV1I GCAGC 1 cut(s) 138
BstXI CCANNNNNNTGG 1 cut(s) 106
BsuRI GGCC 3 cut(s) 36, 184, 220
BtsCI GGATG 1 cut(s) 8
BtsIMutI CAGTG 4 cut(s) 54, 111, 136, 197
Cac8I GCNNGC 1 cut(s) 218
Csp6I GTAC 1 cut(s) 141
CviAII CATG 2 cut(s) 315, 351
CviJI RGCY 7 cut(s) 5, 36, 62, 151, 184, 220, 340
CviKI_1 RGCY 7 cut(s) 5, 36, 62, 151, 184, 220, 340
CviQI GTAC 1 cut(s) 141
DdeI CTNAG 1 cut(s) 189
DpnI GATC 2 cut(s) 147, 297
DpnII GATC 2 cut(s) 145, 295
Eco147I AGGCCT 2 cut(s) 184, 220
FaeI CATG 2 cut(s) 318, 354
FaiI YATR 3 cut(s) 282, 316, 352
FatI CATG 2 cut(s) 314, 350
FbaI TGATCA 1 cut(s) 145
Fnu4HI GCNGC 1 cut(s) 152
Fsp4HI GCNGC 1 cut(s) 152
GluI GCNGC 1 cut(s) 152
HaeIII GGCC 3 cut(s) 36, 184, 220
Hin1II CATG 2 cut(s) 318, 354
HinfI GANTC 2 cut(s) 110, 124
Hpy166II GTNNAC 2 cut(s) 268, 332
Hpy188I TCNGA 1 cut(s) 115
Hpy8I GTNNAC 2 cut(s) 268, 332
HpyCH4III ACNGT 3 cut(s) 58, 131, 272
HpyCH4V TGCA 3 cut(s) 154, 216, 324
HpyF10VI GCNNNNNNNGC 1 cut(s) 222
HpyF3I CTNAG 1 cut(s) 189
Hsp92II CATG 2 cut(s) 318, 354
Ksp22I TGATCA 1 cut(s) 145
Kzo9I GATC 2 cut(s) 145, 295
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 4 cut(s) 28, 39, 158, 202
Lsp1109I GCAGC 1 cut(s) 138
MalI GATC 2 cut(s) 147, 297
MboI GATC 2 cut(s) 145, 295
MboII GAAGA 3 cut(s) 67, 70, 73
MluCI AATT 1 cut(s) 87
MnlI CCTC 4 cut(s) 47, 62, 195, 198
MslI CAYNNNNRTG 2 cut(s) 285, 319
MspA1I CMGCKG 1 cut(s) 151
MwoI GCNNNNNNNGC 1 cut(s) 222
NdeII GATC 2 cut(s) 145, 295
NlaIII CATG 2 cut(s) 318, 354
NlaIV GGNNCC 1 cut(s) 6
PceI AGGCCT 2 cut(s) 184, 220
PfeI GAWTC 2 cut(s) 110, 124
PflMI CCANNNNNTGG 1 cut(s) 32
PkrI GCNGC 1 cut(s) 153
PspN4I GGNNCC 1 cut(s) 6
PstI CTGCAG 1 cut(s) 218
PvuII CAGCTG 1 cut(s) 151
RsaI GTAC 1 cut(s) 142
RsaNI GTAC 1 cut(s) 141
RseI CAYNNNNRTG 2 cut(s) 285, 319
SatI GCNGC 1 cut(s) 152
Sau3AI GATC 2 cut(s) 145, 295
ScaI AGTACT 1 cut(s) 142
SetI ASST 3 cut(s) 153, 267, 342
SfcI CTRYAG 1 cut(s) 214
SgeI CNNG 9 cut(s) 27, 38, 185, 192, 214, 229, 234, 268, 327
SmiMI CAYNNNNRTG 2 cut(s) 285, 319
Sse9I AATT 1 cut(s) 87
SseBI AGGCCT 2 cut(s) 184, 220
StuI AGGCCT 2 cut(s) 184, 220
TaaI ACNGT 3 cut(s) 58, 131, 272
TaqI TCGA 1 cut(s) 122
TasI AATT 1 cut(s) 87
TatI WGTACW 1 cut(s) 140
TfiI GAWTC 2 cut(s) 110, 124
TscAI CASTG 4 cut(s) 61, 111, 136, 197
TseI GCWGC 1 cut(s) 151
TspDTI ATGAA 2 cut(s) 55, 264
TspRI CASTG 4 cut(s) 61, 111, 136, 197
Van91I CCANNNNNTGG 1 cut(s) 32
ZrmI AGTACT 1 cut(s) 142
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.