RLG00000019482

EPIDERMAL PATTERNING FACTOR-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0006184
Physical Location & Seq
Reverse (-)
52586755 .. 52587430
676 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000019482

Sequence Viewer

Length: 351 bp
ATGGCTCCATCCACCAGAAACTATCCAGTAAATGGCCTCAAAGTAGCAAACCTCACTGCGGCTTTCATCTTTTTCTTCTTCACAATTCTATTTTCCAATTCAGTGGATTCCAAACATTCGAGTCATAGTGAAAGCAGTGATATGCTTCAGCAAAGAAAAATGGTTCTGGGTTCAAGGCCTCCTGGATGTGAGAACAAGTGTTTGAATTGCAGGCCTTGCCTTGCTACTCTGGTCATTCCACCTCATCATAAAACAAAGCTTCCCAGTTTGTCATCTCATAATGATCAAGATGATAGCTATTATCTACTCTCATGGAAATGCAAATGTGGACAGAAGCTATTTCAACCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

117

Amino Acids

13.05

Weight (kDa)

9.15

Isoelectric Point (pI)

57.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 55 - 116 2.1e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 32
AciI CCGC 1 cut(s) 59
AcuI CTGAAG 1 cut(s) 131
AfiI CCNNNNNNNGG 2 cut(s) 32, 58
AgsI TTSAA 3 cut(s) 174, 205, 344
AjnI CCWGG 1 cut(s) 181
AluBI AGCT 3 cut(s) 259, 297, 337
AluI AGCT 3 cut(s) 259, 297, 337
AoxI GGCC 3 cut(s) 34, 176, 212
BccI CCATC 1 cut(s) 16
BciT130I CCWGG 1 cut(s) 183
BclI TGATCA 1 cut(s) 283
BisI GCNGC 1 cut(s) 60
BlsI GCNGC 1 cut(s) 61
Bme1390I CCNGG 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 183
BmrI ACTGGG 1 cut(s) 258
BmuI ACTGGG 1 cut(s) 258
Bsc4I CCNNNNNNNGG 2 cut(s) 32, 58
Bse1I ACTGG 2 cut(s) 26, 264
BseBI CCWGG 1 cut(s) 183
BseGI GGATG 2 cut(s) 8, 191
BseLI CCNNNNNNNGG 2 cut(s) 32, 58
BseNI ACTGG 2 cut(s) 26, 264
BshFI GGCC 3 cut(s) 36, 178, 214
BslI CCNNNNNNNGG 2 cut(s) 32, 58
BsnI GGCC 3 cut(s) 36, 178, 214
Bsp143I GATC 1 cut(s) 283
BspACI CCGC 1 cut(s) 59
BspANI GGCC 3 cut(s) 36, 178, 214
BspLI GGNNCC 1 cut(s) 6
BsrI ACTGG 2 cut(s) 26, 264
BssMI GATC 1 cut(s) 283
Bst2UI CCWGG 1 cut(s) 183
BstAPI GCANNNNNTGC 1 cut(s) 216
BstC8I GCNNGC 1 cut(s) 212
BstF5I GGATG 2 cut(s) 8, 191
BstKTI GATC 1 cut(s) 286
BstMBI GATC 1 cut(s) 283
BstMWI GCNNNNNNNGC 1 cut(s) 216
BstNI CCWGG 1 cut(s) 183
BstSCI CCNGG 1 cut(s) 181
BstXI CCANNNNNNTGG 1 cut(s) 103
BsuRI GGCC 3 cut(s) 36, 178, 214
BtsCI GGATG 2 cut(s) 8, 191
BtsI GCAGTG 2 cut(s) 54, 142
BtsIMutI CAGTG 3 cut(s) 54, 108, 142
Cac8I GCNNGC 1 cut(s) 212
CviAII CATG 2 cut(s) 312, 348
CviJI RGCY 8 cut(s) 5, 36, 62, 178, 214, 259, 297, 337
CviKI_1 RGCY 8 cut(s) 5, 36, 62, 178, 214, 259, 297, 337
DpnI GATC 1 cut(s) 285
DpnII GATC 1 cut(s) 283
Eco147I AGGCCT 2 cut(s) 178, 214
Eco57I CTGAAG 1 cut(s) 131
EcoRII CCWGG 1 cut(s) 181
FaeI CATG 2 cut(s) 315, 351
FaiI YATR 6 cut(s) 126, 143, 249, 279, 313, 349
FatI CATG 2 cut(s) 311, 347
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 1 cut(s) 60
FokI GGATG 1 cut(s) 198
Fsp4HI GCNGC 1 cut(s) 60
GluI GCNGC 1 cut(s) 60
HaeIII GGCC 3 cut(s) 36, 178, 214
Hin1II CATG 2 cut(s) 315, 351
HindIII AAGCTT 1 cut(s) 257
HinfI GANTC 2 cut(s) 107, 121
Hpy166II GTNNAC 1 cut(s) 329
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 1 cut(s) 329
HpyCH4V TGCA 2 cut(s) 210, 321
HpyF10VI GCNNNNNNNGC 1 cut(s) 216
Hsp92II CATG 2 cut(s) 315, 351
Ksp22I TGATCA 1 cut(s) 283
Kzo9I GATC 1 cut(s) 283
LmnI GCTCC 1 cut(s) 10
LpnPI CCDG 8 cut(s) 28, 39, 152, 168, 195, 196, 215, 277
MalI GATC 1 cut(s) 285
MboI GATC 1 cut(s) 283
MboII GAAGA 2 cut(s) 67, 70
MluCI AATT 3 cut(s) 84, 97, 205
MlyI GAGTC 1 cut(s) 130
MnlI CCTC 4 cut(s) 47, 62, 189, 252
MslI CAYNNNNRTG 1 cut(s) 316
MspR9I CCNGG 1 cut(s) 183
MvaI CCWGG 1 cut(s) 183
MwoI GCNNNNNNNGC 1 cut(s) 216
NdeII GATC 1 cut(s) 283
NlaIII CATG 2 cut(s) 315, 351
NlaIV GGNNCC 1 cut(s) 6
PceI AGGCCT 2 cut(s) 178, 214
PfeI GAWTC 1 cut(s) 107
PflMI CCANNNNNTGG 1 cut(s) 32
PfoI TCCNGGA 1 cut(s) 181
PkrI GCNGC 1 cut(s) 61
PleI GAGTC 1 cut(s) 129
PpsI GAGTC 1 cut(s) 129
Psp6I CCWGG 1 cut(s) 181
PspGI CCWGG 1 cut(s) 181
PspN4I GGNNCC 1 cut(s) 6
RseI CAYNNNNRTG 1 cut(s) 316
SatI GCNGC 1 cut(s) 60
Sau3AI GATC 1 cut(s) 283
SchI GAGTC 1 cut(s) 130
ScrFI CCNGG 1 cut(s) 183
SetI ASST 5 cut(s) 54, 244, 261, 299, 339
SmiMI CAYNNNNRTG 1 cut(s) 316
Sse9I AATT 3 cut(s) 84, 97, 205
SseBI AGGCCT 2 cut(s) 178, 214
SsiI CCGC 1 cut(s) 59
StuI AGGCCT 2 cut(s) 178, 214
StyD4I CCNGG 1 cut(s) 181
TaqI TCGA 1 cut(s) 119
TasI AATT 3 cut(s) 84, 97, 205
TauI GCSGC 1 cut(s) 62
TfiI GAWTC 1 cut(s) 107
TscAI CASTG 3 cut(s) 61, 108, 142
TspDTI ATGAA 1 cut(s) 55
TspRI CASTG 3 cut(s) 61, 108, 142
Van91I CCANNNNNTGG 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.