MD02G1030200.v1.1

Epidermal patterning factor proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006184
Physical Location & Seq
Reverse (-)
2333786 .. 2334687
902 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1030200.v1.1.491

Sequence Viewer

Length: 336 bp
ATGGCTTCTTCAAGAAATCACCCCAATGGGTTAAAGATCACAGTCGCTGTCATGTTTCTCATCTTTTTTCTCACATTCTTTCCTTCCATTTCAGCTGAGTCAATGTTGAAGAGAGCTGGTGATCAACGGTTAAAAAATCAAATGAAGATGGTGCTGGGATCGAGTCCGCCGCGGTGCGTGAACAAATGCATGAGTTGCAGGCCTTGTACGGCCACTCTCGTTGCTTCTTCGCACCACAAGAGTAGTAAACTCAAGGCAGCAACTTACCAGGGAGATGAGAGCTATTATCTCCTCTCATGGAAATGCAGATGTAGGAACAAGTTGTACCAACCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

112

Amino Acids

12.54

Weight (kDa)

9.94

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 51 - 111 3.4e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 172
AciI CCGC 3 cut(s) 167, 170, 172
AclWI GGATC 1 cut(s) 166
AcoI YGGCCR 1 cut(s) 210
AfaI GTAC 2 cut(s) 208, 326
AgsI TTSAA 2 cut(s) 12, 109
AjnI CCWGG 1 cut(s) 267
AluBI AGCT 3 cut(s) 95, 116, 282
AluI AGCT 3 cut(s) 95, 116, 282
AlwI GGATC 1 cut(s) 166
AlwNI CAGNNNCTG 1 cut(s) 47
AoxI GGCC 2 cut(s) 200, 210
ApeKI GCWGC 1 cut(s) 257
AsuHPI GGTGA 2 cut(s) 11, 131
BbvI GCAGC 1 cut(s) 269
BccI CCATC 1 cut(s) 142
BceAI ACGGC 1 cut(s) 225
BciT130I CCWGG 1 cut(s) 269
BclI TGATCA 1 cut(s) 121
BisI GCNGC 2 cut(s) 170, 258
BlsI GCNGC 2 cut(s) 171, 259
Bme1390I CCNGG 1 cut(s) 269
BmrFI CCNGG 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 236
BsaJI CCNNGG 2 cut(s) 170, 268
BseBI CCWGG 1 cut(s) 269
BseDI CCNNGG 2 cut(s) 170, 268
BseMII CTCAG 1 cut(s) 87
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 269
BseYI CCCAGC 1 cut(s) 154
Bsh1236I CGCG 1 cut(s) 172
BshFI GGCC 2 cut(s) 202, 212
BsnI GGCC 2 cut(s) 202, 212
Bsp143I GATC 3 cut(s) 36, 121, 158
BspACI CCGC 3 cut(s) 167, 170, 172
BspANI GGCC 2 cut(s) 202, 212
BspCNI CTCAG 1 cut(s) 88
BspFNI CGCG 1 cut(s) 172
BspPI GGATC 1 cut(s) 166
BssECI CCNNGG 2 cut(s) 170, 268
BssMI GATC 3 cut(s) 36, 121, 158
Bst2UI CCWGG 1 cut(s) 269
Bst4CI ACNGT 2 cut(s) 43, 129
Bst6I CTCTTC 1 cut(s) 104
BstAPI GCANNNNNTGC 1 cut(s) 195
BstC8I GCNNGC 1 cut(s) 200
BstDEI CTNAG 1 cut(s) 96
BstDSI CCRYGG 1 cut(s) 170
BstFNI CGCG 1 cut(s) 172
BstKTI GATC 3 cut(s) 39, 124, 161
BstMBI GATC 3 cut(s) 36, 121, 158
BstMWI GCNNNNNNNGC 1 cut(s) 195
BstNI CCWGG 1 cut(s) 269
BstSCI CCNGG 1 cut(s) 267
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 269
BsuRI GGCC 2 cut(s) 202, 212
BtgI CCRYGG 1 cut(s) 170
Cac8I GCNNGC 1 cut(s) 200
CaiI CAGNNNCTG 1 cut(s) 47
Cfr42I CCGCGG 1 cut(s) 173
Csp6I GTAC 2 cut(s) 207, 325
CspCI CAANNNNNGTGG 2 cut(s) 202, 237
CviAII CATG 3 cut(s) 52, 190, 297
CviJI RGCY 6 cut(s) 5, 95, 116, 202, 212, 282
CviKI_1 RGCY 6 cut(s) 5, 95, 116, 202, 212, 282
CviQI GTAC 2 cut(s) 207, 325
DdeI CTNAG 1 cut(s) 96
DpnI GATC 3 cut(s) 38, 123, 160
DpnII GATC 3 cut(s) 36, 121, 158
EaeI YGGCCR 1 cut(s) 210
Eam1104I CTCTTC 1 cut(s) 104
EarI CTCTTC 1 cut(s) 104
EciI GGCGGA 1 cut(s) 156
Eco147I AGGCCT 1 cut(s) 202
EcoRII CCWGG 1 cut(s) 267
EcoT22I ATGCAT 1 cut(s) 191
FaeI CATG 3 cut(s) 55, 193, 300
FaiI YATR 3 cut(s) 53, 191, 298
FatI CATG 3 cut(s) 51, 189, 296
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 2 cut(s) 170, 258
Fsp4HI GCNGC 2 cut(s) 170, 258
GluI GCNGC 2 cut(s) 170, 258
GsaI CCCAGC 1 cut(s) 158
HaeIII GGCC 2 cut(s) 202, 212
Hin1II CATG 3 cut(s) 55, 193, 300
HinfI GANTC 2 cut(s) 98, 163
HphI GGTGA 2 cut(s) 11, 131
Hpy166II GTNNAC 2 cut(s) 181, 248
Hpy188III TCNNGA 1 cut(s) 12
Hpy8I GTNNAC 2 cut(s) 181, 248
HpyAV CCTTC 1 cut(s) 93
HpyCH4III ACNGT 2 cut(s) 43, 129
HpyCH4V TGCA 3 cut(s) 189, 198, 306
HpyF10VI GCNNNNNNNGC 1 cut(s) 195
HpyF3I CTNAG 1 cut(s) 96
Hsp92II CATG 3 cut(s) 55, 193, 300
Ksp22I TGATCA 1 cut(s) 121
KspI CCGCGG 1 cut(s) 173
Kzo9I GATC 3 cut(s) 36, 121, 158
LpnPI CCDG 5 cut(s) 102, 140, 184, 254, 281
Lsp1109I GCAGC 1 cut(s) 269
MalI GATC 3 cut(s) 38, 123, 160
MboI GATC 3 cut(s) 36, 121, 158
MboII GAAGA 3 cut(s) 121, 157, 219
MlyI GAGTC 2 cut(s) 107, 172
MnlI CCTC 1 cut(s) 302
Mph1103I ATGCAT 1 cut(s) 191
MseI TTAA 2 cut(s) 32, 131
MslI CAYNNNNRTG 2 cut(s) 24, 301
MspA1I CMGCKG 2 cut(s) 95, 172
MspR9I CCNGG 1 cut(s) 269
MvaI CCWGG 1 cut(s) 269
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 195
NdeII GATC 3 cut(s) 36, 121, 158
NlaIII CATG 3 cut(s) 55, 193, 300
NsiI ATGCAT 1 cut(s) 191
PceI AGGCCT 1 cut(s) 202
PkrI GCNGC 2 cut(s) 171, 259
PleI GAGTC 2 cut(s) 106, 171
PpsI GAGTC 2 cut(s) 106, 171
Psp6I CCWGG 1 cut(s) 267
PspFI CCCAGC 1 cut(s) 154
PspGI CCWGG 1 cut(s) 267
PsrI GAACNNNNNNTAC 1 cut(s) 308
PstNI CAGNNNCTG 1 cut(s) 47
PvuII CAGCTG 1 cut(s) 95
RsaI GTAC 2 cut(s) 208, 326
RsaNI GTAC 2 cut(s) 207, 325
RseI CAYNNNNRTG 2 cut(s) 24, 301
SacII CCGCGG 1 cut(s) 173
SaqAI TTAA 2 cut(s) 32, 131
SatI GCNGC 2 cut(s) 170, 258
Sau3AI GATC 3 cut(s) 36, 121, 158
SchI GAGTC 2 cut(s) 107, 172
ScrFI CCNGG 1 cut(s) 269
SetI ASST 4 cut(s) 97, 118, 284, 334
Sfr303I CCGCGG 1 cut(s) 173
SgrBI CCGCGG 1 cut(s) 173
SmiMI CAYNNNNRTG 2 cut(s) 24, 301
SmlI CTYRAG 1 cut(s) 251
SmoI CTYRAG 1 cut(s) 251
SseBI AGGCCT 1 cut(s) 202
SsiI CCGC 3 cut(s) 167, 170, 172
StuI AGGCCT 1 cut(s) 202
StyD4I CCNGG 1 cut(s) 267
TaaI ACNGT 2 cut(s) 43, 129
TaqI TCGA 1 cut(s) 161
TauI GCSGC 1 cut(s) 172
Tru1I TTAA 2 cut(s) 32, 131
Tru9I TTAA 2 cut(s) 32, 131
TseI GCWGC 1 cut(s) 257
TspDTI ATGAA 1 cut(s) 158
Zsp2I ATGCAT 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.