MD15G1171700.v1.1

Epidermal patterning factor proteins

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0006184
Physical Location & Seq
Reverse (-)
13331192 .. 13332049
858 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1171700.v1.1.491

Sequence Viewer

Length: 324 bp
ATGGCTTCTTTAAGAAAATGCCCAAATGGGTTAAAAATCACTGTCATTATCTTGTTTCTCACATTCTTTCCTTCCATTTCAGCTGAGTCCATGTTGAAGAGAGATGGTGATCAACGATTAAAAAATCAAATGAAGACGGTGCTGGGATCGAGGCCGCCGCAATGTGCGAACAAATGCATGAGTTGCAGGCCTTGTACGGCCACTCTGGTTGCTTCTTCACACCACAAGAGCAGTAATCTCGAGGCAACAACTTATCAGGGAGATGAGAACTATTATCTCCTCTCTTGGAAATGCAGATGCAGGAACAAGTTGTTCCATCCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
EPF

Protein Analysis

108

Amino Acids

12.13

Weight (kDa)

9.6

Isoelectric Point (pI)

53.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 47 - 107 1.5e-20 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 155, 158
AclWI GGATC 1 cut(s) 154
AcoI YGGCCR 1 cut(s) 198
AfaI GTAC 1 cut(s) 196
AgsI TTSAA 1 cut(s) 97
AluBI AGCT 1 cut(s) 83
AluI AGCT 1 cut(s) 83
AlwI GGATC 1 cut(s) 154
Ama87I CYCGRG 1 cut(s) 239
AoxI GGCC 3 cut(s) 152, 188, 198
AsuHPI GGTGA 1 cut(s) 119
AvaI CYCGRG 1 cut(s) 239
BbsI GAAGAC 1 cut(s) 140
BccI CCATC 1 cut(s) 98
BceAI ACGGC 1 cut(s) 213
BcgI CGANNNNNNTGC 2 cut(s) 220, 254
BclI TGATCA 1 cut(s) 109
BisI GCNGC 2 cut(s) 155, 158
BlsI GCNGC 2 cut(s) 156, 159
BmeT110I CYCGRG 1 cut(s) 239
BmsI GCATC 1 cut(s) 287
BpiI GAAGAC 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 108
Bse3DI GCAATG 1 cut(s) 167
Bse8I GATNNNNATC 1 cut(s) 108
BseGI GGATG 1 cut(s) 316
BseJI GATNNNNATC 1 cut(s) 108
BseMI GCAATG 1 cut(s) 167
BseMII CTCAG 1 cut(s) 75
BseRI GAGGAG 1 cut(s) 269
BseYI CCCAGC 1 cut(s) 142
BshFI GGCC 3 cut(s) 154, 190, 200
BsiHKCI CYCGRG 1 cut(s) 239
BsnI GGCC 3 cut(s) 154, 190, 200
BsoBI CYCGRG 1 cut(s) 239
Bsp143I GATC 2 cut(s) 109, 146
BspACI CCGC 2 cut(s) 155, 158
BspANI GGCC 3 cut(s) 154, 190, 200
BspCNI CTCAG 1 cut(s) 76
BspPI GGATC 1 cut(s) 154
BsrDI GCAATG 1 cut(s) 167
BssMI GATC 2 cut(s) 109, 146
Bst4CI ACNGT 2 cut(s) 43, 139
Bst6I CTCTTC 1 cut(s) 92
BstAPI GCANNNNNTGC 1 cut(s) 183
BstC8I GCNNGC 1 cut(s) 188
BstDEI CTNAG 1 cut(s) 84
BstF5I GGATG 1 cut(s) 316
BstKTI GATC 2 cut(s) 112, 149
BstMBI GATC 2 cut(s) 109, 146
BstMWI GCNNNNNNNGC 1 cut(s) 183
BstV2I GAAGAC 1 cut(s) 140
BsuRI GGCC 3 cut(s) 154, 190, 200
BtsCI GGATG 1 cut(s) 316
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 1 cut(s) 188
Csp6I GTAC 1 cut(s) 195
CspCI CAANNNNNGTGG 2 cut(s) 190, 225
CviAII CATG 2 cut(s) 91, 178
CviJI RGCY 5 cut(s) 5, 83, 154, 190, 200
CviKI_1 RGCY 5 cut(s) 5, 83, 154, 190, 200
CviQI GTAC 1 cut(s) 195
DdeI CTNAG 1 cut(s) 84
DpnI GATC 2 cut(s) 111, 148
DpnII GATC 2 cut(s) 109, 146
EaeI YGGCCR 1 cut(s) 198
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco147I AGGCCT 1 cut(s) 190
Eco88I CYCGRG 1 cut(s) 239
EcoT22I ATGCAT 1 cut(s) 179
FaeI CATG 2 cut(s) 94, 181
FaiI YATR 2 cut(s) 92, 179
FatI CATG 2 cut(s) 90, 177
FbaI TGATCA 1 cut(s) 109
Fnu4HI GCNGC 2 cut(s) 155, 158
FokI GGATG 1 cut(s) 303
Fsp4HI GCNGC 2 cut(s) 155, 158
GluI GCNGC 2 cut(s) 155, 158
GsaI CCCAGC 1 cut(s) 146
HaeIII GGCC 3 cut(s) 154, 190, 200
Hin1II CATG 2 cut(s) 94, 181
HinfI GANTC 1 cut(s) 86
HphI GGTGA 1 cut(s) 119
Hpy188III TCNNGA 1 cut(s) 239
HpyAV CCTTC 1 cut(s) 81
HpyCH4III ACNGT 2 cut(s) 43, 139
HpyCH4V TGCA 4 cut(s) 177, 186, 294, 300
HpyF10VI GCNNNNNNNGC 1 cut(s) 183
HpyF3I CTNAG 1 cut(s) 84
Hsp92II CATG 2 cut(s) 94, 181
Ksp22I TGATCA 1 cut(s) 109
Kzo9I GATC 2 cut(s) 109, 146
LpnPI CCDG 5 cut(s) 128, 172, 191, 242, 286
LweI GCATC 1 cut(s) 287
MalI GATC 2 cut(s) 111, 148
MboI GATC 2 cut(s) 109, 146
MboII GAAGA 3 cut(s) 109, 145, 207
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 3 cut(s) 144, 235, 290
Mph1103I ATGCAT 1 cut(s) 179
MseI TTAA 3 cut(s) 11, 32, 119
MspA1I CMGCKG 1 cut(s) 83
MwoI GCNNNNNNNGC 1 cut(s) 183
NdeII GATC 2 cut(s) 109, 146
NlaIII CATG 2 cut(s) 94, 181
NsiI ATGCAT 1 cut(s) 179
PaeR7I CTCGAG 1 cut(s) 239
PceI AGGCCT 1 cut(s) 190
PkrI GCNGC 2 cut(s) 156, 159
PleI GAGTC 1 cut(s) 94
PpsI GAGTC 1 cut(s) 94
PspFI CCCAGC 1 cut(s) 142
PvuII CAGCTG 1 cut(s) 83
RsaI GTAC 1 cut(s) 196
RsaNI GTAC 1 cut(s) 195
SaqAI TTAA 3 cut(s) 11, 32, 119
SatI GCNGC 2 cut(s) 155, 158
Sau3AI GATC 2 cut(s) 109, 146
SchI GAGTC 1 cut(s) 95
SetI ASST 1 cut(s) 85
SfaNI GCATC 1 cut(s) 287
Sfr274I CTCGAG 1 cut(s) 239
SlaI CTCGAG 1 cut(s) 239
SmlI CTYRAG 1 cut(s) 239
SmoI CTYRAG 1 cut(s) 239
SseBI AGGCCT 1 cut(s) 190
SsiI CCGC 2 cut(s) 155, 158
StuI AGGCCT 1 cut(s) 190
TaaI ACNGT 2 cut(s) 43, 139
TaqI TCGA 2 cut(s) 149, 240
TauI GCSGC 2 cut(s) 157, 160
Tru1I TTAA 3 cut(s) 11, 32, 119
Tru9I TTAA 3 cut(s) 11, 32, 119
TscAI CASTG 1 cut(s) 46
TspDTI ATGAA 1 cut(s) 146
TspRI CASTG 1 cut(s) 46
XhoI CTCGAG 1 cut(s) 239
Zsp2I ATGCAT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.