FvH4_6g30051

Nucleosome assembly protein (NAP)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0016369
Physical Location & Seq
Forward (+)
23203494 .. 23205949
2456 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g30051.t1

Sequence Viewer

Length: 717 bp
ATGACCAGCAACAGAGATAAACTCGAACGCAGTGTCTCTCGCATCCCTACAGCCGCTGCTCTCAGTGCCAAGGATCGTGCTGCTCTTGTTAAAGCTCTCGAGAGCTTGGTTGGACAGCATTCTGGCAAAGTTGAATCACCCAAGATGAAGAAGCGCATTAAGGCTTTGACTGAAATACAGGGCCAACTCAAGGACCTGAACACAAAATTTGTTGAGGAGAGAGCTGCACTGAAAGCTAAACAGAATCGTTGCCGAGAAGAGGGACACAACTTTTGGCTTTATTCACTGAAGAATAATGAAGTGCTTGCTAAGGAGATCACAGAACAAGATGAAGAAGCTCTCAAGGATCTCAAAGATATCAAGTGCTGTAGGATAGGAAACTCTAAGGGATTGAAGATGGAGTTTATCTTCAGTTATAGCTCCTATTTTGAAAATTCTGCATTGATAAAAACGTACCACATGACTAATGACAATAAGCCTATCTTGGAGAAGGCCATTGGGACGAAGATCAAGTGGTATCCCGGGAAAATTTTGACAGAGAACCGTCACAGCTTCTTCAACTTCTTTCAACCTCTCCAGGCTGCAGATGTCCAAAATCGGATGCACCGTGATTATGATATTTTTTCAAAAATTTGGAATGAAATTATCCCTCAGTGGCACAAAGTGAAAAAGATTTCTAGTAATTCATTCAACTATGGAGTGGACGGACTAAAATAA

Protein Analysis

239

Amino Acids

27.52

Weight (kDa)

9.55

Isoelectric Point (pI)

34.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAP PF00956 89 - 180 9.9e-25 Nucleosome assembly protein (NAP)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0016369)

Species Orthologous Gene IDs
fragaria_vesca FvH4_6g30051 FvH4_6g30060 FvH4_6g30063
rosa_chinensis RchiOBHm_Chr2g0136101
rosa_laevigata RLG00000019503
rosa_multiflora Rmu_sc0002070.1_g000009 Rmu_sc0002986.1_g000017
rosa_roxburghii Rroxscaffold_2G00108490
rosa_rugosa Rorug02G0328000 Rorug02G0328100
rosa_samantha Rh2AG379800 Rh2BG386600 Rh2CG365800 Rh2DG402700
rosa_wichuraiana Rw2G030960

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 54
AclWI GGATC 2 cut(s) 81, 354
AcsI RAATTY 4 cut(s) 206, 433, 528, 630
AcuI CTGAAG 2 cut(s) 308, 394
AdeI CACNNNGTG 1 cut(s) 664
AfaI GTAC 1 cut(s) 455
AfiI CCNNNNNNNGG 2 cut(s) 190, 259
AgsI TTSAA 7 cut(s) 134, 394, 431, 559, 569, 627, 691
AjnI CCWGG 1 cut(s) 576
AluBI AGCT 7 cut(s) 95, 105, 224, 236, 338, 420, 552
AluI AGCT 7 cut(s) 95, 105, 224, 236, 338, 420, 552
Alw26I GTCTC 1 cut(s) 40
AlwI GGATC 2 cut(s) 81, 354
AlwNI CAGNNNCTG 1 cut(s) 56
Ama87I CYCGRG 2 cut(s) 98, 521
AoxI GGCC 2 cut(s) 181, 492
ApeKI GCWGC 4 cut(s) 56, 80, 224, 581
ApoI RAATTY 4 cut(s) 206, 433, 528, 630
ArsI GACNNNNNNTTYG 4 cut(s) 18, 50, 255, 287
AspLEI GCGC 1 cut(s) 156
AspS9I GGNCC 2 cut(s) 181, 193
AsuC2I CCSGG 2 cut(s) 522, 523
AsuHPI GGTGA 1 cut(s) 129
AvaI CYCGRG 2 cut(s) 98, 521
AvaII GGWCC 1 cut(s) 193
BbvI GCAGC 4 cut(s) 43, 67, 211, 568
BccI CCATC 1 cut(s) 391
BciT130I CCWGG 1 cut(s) 578
BciVI GTATCC 1 cut(s) 528
BcnI CCSGG 2 cut(s) 522, 523
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 678
BfmI CTRYAG 3 cut(s) 48, 367, 582
BfuI GTATCC 1 cut(s) 528
BisI GCNGC 5 cut(s) 54, 57, 81, 225, 582
BlsI GCNGC 5 cut(s) 55, 58, 82, 226, 583
Bme1390I CCNGG 3 cut(s) 522, 523, 578
Bme18I GGWCC 1 cut(s) 193
BmeT110I CYCGRG 2 cut(s) 98, 521
BmgT120I GGNCC 2 cut(s) 181, 193
BmrFI CCNGG 3 cut(s) 522, 523, 578
BmsI GCATC 2 cut(s) 51, 591
BplI GAGNNNNNCTC 1 cut(s) 38
BpmI CTGGAG 1 cut(s) 560
Bpu10I CCTNAGC 1 cut(s) 309
BpuEI CTTGAG 2 cut(s) 173, 326
BpuMI CCSGG 2 cut(s) 522, 523
BsaJI CCNNGG 2 cut(s) 69, 521
Bsc4I CCNNNNNNNGG 2 cut(s) 190, 259
BseBI CCWGG 1 cut(s) 578
BseDI CCNNGG 2 cut(s) 69, 521
BseGI GGATG 2 cut(s) 42, 606
BseLI CCNNNNNNNGG 2 cut(s) 190, 259
BseMII CTCAG 2 cut(s) 76, 665
BseRI GAGGAG 1 cut(s) 230
BseXI GCAGC 4 cut(s) 43, 67, 211, 568
BsgI GTGCAG 1 cut(s) 210
BshFI GGCC 2 cut(s) 183, 494
BsiHKCI CYCGRG 2 cut(s) 98, 521
BsiSI CCGG 1 cut(s) 522
BslFI GGGAC 2 cut(s) 276, 514
BslI CCNNNNNNNGG 2 cut(s) 190, 259
BsmAI GTCTC 1 cut(s) 40
BsmFI GGGAC 2 cut(s) 276, 514
BsmI GAATGC 1 cut(s) 118
BsnI GGCC 2 cut(s) 183, 494
BsoBI CYCGRG 2 cut(s) 98, 521
Bsp143I GATC 4 cut(s) 73, 315, 346, 507
BspACI CCGC 1 cut(s) 54
BspANI GGCC 2 cut(s) 183, 494
BspCNI CTCAG 2 cut(s) 75, 664
BspMAI CTGCAG 1 cut(s) 586
BspPI GGATC 2 cut(s) 81, 354
BssECI CCNNGG 2 cut(s) 69, 521
BssMI GATC 4 cut(s) 73, 315, 346, 507
BssT1I CCWWGG 1 cut(s) 69
Bst2UI CCWGG 1 cut(s) 578
Bst4CI ACNGT 2 cut(s) 545, 608
Bst6I CTCTTC 1 cut(s) 252
BstC8I GCNNGC 1 cut(s) 306
BstDEI CTNAG 4 cut(s) 62, 309, 384, 651
BstF5I GGATG 2 cut(s) 42, 606
BstHHI GCGC 1 cut(s) 156
BstKTI GATC 4 cut(s) 76, 318, 349, 510
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 4 cut(s) 73, 315, 346, 507
BstMWI GCNNNNNNNGC 2 cut(s) 65, 233
BstNI CCWGG 1 cut(s) 578
BstSCI CCNGG 3 cut(s) 520, 521, 576
BstSFI CTRYAG 3 cut(s) 48, 367, 582
BstV1I GCAGC 4 cut(s) 43, 67, 211, 568
BstX2I RGATCY 1 cut(s) 346
BstYI RGATCY 1 cut(s) 346
BsuI GTATCC 1 cut(s) 528
BsuRI GGCC 2 cut(s) 183, 494
BtsCI GGATG 2 cut(s) 42, 606
BtsI GCAGTG 1 cut(s) 37
BtsIMutI CAGTG 5 cut(s) 37, 70, 227, 284, 659
Cac8I GCNNGC 1 cut(s) 306
CaiI CAGNNNCTG 1 cut(s) 56
CfoI GCGC 1 cut(s) 156
Cfr13I GGNCC 2 cut(s) 181, 193
Cfr9I CCCGGG 1 cut(s) 521
Csp6I GTAC 1 cut(s) 454
CviAII CATG 1 cut(s) 460
CviQI GTAC 1 cut(s) 454
DdeI CTNAG 4 cut(s) 62, 309, 384, 651
DpnI GATC 4 cut(s) 75, 317, 348, 509
DpnII GATC 4 cut(s) 73, 315, 346, 507
DraIII CACNNNGTG 1 cut(s) 664
Eam1104I CTCTTC 1 cut(s) 252
EarI CTCTTC 1 cut(s) 252
Eco130I CCWWGG 1 cut(s) 69
Eco32I GATATC 1 cut(s) 358
Eco47I GGWCC 1 cut(s) 193
Eco57I CTGAAG 2 cut(s) 308, 394
Eco88I CYCGRG 2 cut(s) 98, 521
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 576
EcoRV GATATC 1 cut(s) 358
EcoT14I CCWWGG 1 cut(s) 69
ErhI CCWWGG 1 cut(s) 69
FaeI CATG 1 cut(s) 463
FaiI YATR 4 cut(s) 417, 461, 615, 696
FalI AAGNNNNNCTT 2 cut(s) 467, 499
FaqI GGGAC 2 cut(s) 276, 514
FatI CATG 1 cut(s) 459
Fnu4HI GCNGC 5 cut(s) 54, 57, 81, 225, 582
FokI GGATG 2 cut(s) 29, 613
Fsp4HI GCNGC 5 cut(s) 54, 57, 81, 225, 582
FspBI CTAG 1 cut(s) 678
GlaI GCGC 1 cut(s) 155
GluI GCNGC 5 cut(s) 54, 57, 81, 225, 582
GsuI CTGGAG 1 cut(s) 560
HaeIII GGCC 2 cut(s) 183, 494
HapII CCGG 1 cut(s) 522
HhaI GCGC 1 cut(s) 156
Hin1II CATG 1 cut(s) 463
Hin6I GCGC 1 cut(s) 154
HinP1I GCGC 1 cut(s) 154
HinfI GANTC 2 cut(s) 134, 244
HpaII CCGG 1 cut(s) 522
HphI GGTGA 1 cut(s) 129
Hpy166II GTNNAC 1 cut(s) 703
Hpy188I TCNGA 1 cut(s) 600
Hpy188III TCNNGA 2 cut(s) 98, 100
Hpy8I GTNNAC 1 cut(s) 703
HpyAV CCTTC 1 cut(s) 484
HpyCH4III ACNGT 2 cut(s) 545, 608
HpyCH4IV ACGT 1 cut(s) 452
HpyCH4V TGCA 4 cut(s) 227, 440, 584, 604
HpyF10VI GCNNNNNNNGC 2 cut(s) 65, 233
HpyF3I CTNAG 4 cut(s) 62, 309, 384, 651
HpySE526I ACGT 1 cut(s) 452
Hsp92II CATG 1 cut(s) 463
HspAI GCGC 1 cut(s) 154
Kzo9I GATC 4 cut(s) 73, 315, 346, 507
LmnI GCTCC 1 cut(s) 425
LpnPI CCDG 7 cut(s) 19, 108, 164, 209, 535, 563, 590
Lsp1109I GCAGC 4 cut(s) 43, 67, 211, 568
LweI GCATC 2 cut(s) 51, 591
MaeI CTAG 1 cut(s) 678
MaeII ACGT 1 cut(s) 452
MaeIII GTNAC 1 cut(s) 545
MalI GATC 4 cut(s) 75, 317, 348, 509
MboI GATC 4 cut(s) 73, 315, 346, 507
MboII GAAGA 8 cut(s) 160, 269, 301, 344, 400, 406, 517, 547
MflI RGATCY 1 cut(s) 346
MluCI AATT 6 cut(s) 206, 433, 528, 630, 642, 682
MmeI TCCRAC 1 cut(s) 91
MnlI CCTC 4 cut(s) 208, 253, 582, 660
MseI TTAA 2 cut(s) 90, 159
MspA1I CMGCKG 1 cut(s) 56
MspI CCGG 1 cut(s) 522
MspR9I CCNGG 3 cut(s) 522, 523, 578
Mva1269I GAATGC 1 cut(s) 118
MvaI CCWGG 1 cut(s) 578
MwoI GCNNNNNNNGC 2 cut(s) 65, 233
NciI CCSGG 2 cut(s) 522, 523
NdeII GATC 4 cut(s) 73, 315, 346, 507
NlaIII CATG 1 cut(s) 463
NmeAIII GCCGAG 1 cut(s) 278
NmuCI GTSAC 1 cut(s) 545
PaeR7I CTCGAG 1 cut(s) 98
PcsI WCGNNNNNNNCGW 1 cut(s) 604
PctI GAATGC 1 cut(s) 118
PfeI GAWTC 2 cut(s) 134, 244
PkrI GCNGC 5 cut(s) 55, 58, 82, 226, 583
PpuMI RGGWCCY 1 cut(s) 193
Psp5II RGGWCCY 1 cut(s) 193
Psp6I CCWGG 1 cut(s) 576
PspGI CCWGG 1 cut(s) 576
PspPI GGNCC 2 cut(s) 181, 193
PspPPI RGGWCCY 1 cut(s) 193
PstI CTGCAG 1 cut(s) 586
PstNI CAGNNNCTG 1 cut(s) 56
PsuI RGATCY 1 cut(s) 346
RsaI GTAC 1 cut(s) 455
RsaNI GTAC 1 cut(s) 454
SaqAI TTAA 2 cut(s) 90, 159
SatI GCNGC 5 cut(s) 54, 57, 81, 225, 582
Sau3AI GATC 4 cut(s) 73, 315, 346, 507
Sau96I GGNCC 2 cut(s) 181, 193
ScrFI CCNGG 3 cut(s) 522, 523, 578
SfaNI GCATC 2 cut(s) 51, 591
SfcI CTRYAG 3 cut(s) 48, 367, 582
Sfr274I CTCGAG 1 cut(s) 98
SinI GGWCC 1 cut(s) 193
SlaI CTCGAG 1 cut(s) 98
SmaI CCCGGG 1 cut(s) 523
SmlI CTYRAG 3 cut(s) 98, 188, 341
SmoI CTYRAG 3 cut(s) 98, 188, 341
Sse9I AATT 6 cut(s) 206, 433, 528, 630, 642, 682
SsiI CCGC 1 cut(s) 54
SspMI CTAG 1 cut(s) 678
StyD4I CCNGG 3 cut(s) 520, 521, 576
StyI CCWWGG 1 cut(s) 69
TaaI ACNGT 2 cut(s) 545, 608
TaiI ACGT 1 cut(s) 455
TaqI TCGA 2 cut(s) 24, 99
TasI AATT 6 cut(s) 206, 433, 528, 630, 642, 682
TauI GCSGC 1 cut(s) 56
TfiI GAWTC 2 cut(s) 134, 244
Tru1I TTAA 2 cut(s) 90, 159
Tru9I TTAA 2 cut(s) 90, 159
TscAI CASTG 5 cut(s) 37, 70, 234, 291, 659
TseFI GTSAC 1 cut(s) 545
TseI GCWGC 4 cut(s) 56, 80, 224, 581
Tsp45I GTSAC 1 cut(s) 545
TspDTI ATGAA 5 cut(s) 161, 312, 345, 654, 675
TspMI CCCGGG 1 cut(s) 521
TspRI CASTG 5 cut(s) 37, 70, 234, 291, 659
VpaK11BI GGWCC 1 cut(s) 193
XapI RAATTY 4 cut(s) 206, 433, 528, 630
XhoI CTCGAG 1 cut(s) 98
XmaI CCCGGG 1 cut(s) 521
XspI CTAG 1 cut(s) 678
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.