FvH4_6g43350

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008878
Physical Location & Seq
Forward (+)
33561194 .. 33562204
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43350.t1

Sequence Viewer

Length: 567 bp
ATGGCTTCCAATTTTATACCAAGGTCGACCACTAATACTTATCTCTCTCGAACCAGTCTAGTGTTTCTCCTCGTTCTGCTCATGACTATATTTTTGGTCGAAGCTGCACATATTGAGCAGTCCCGGTTTGAGAGGAATTTCGAGCTGAAGTACTACGCCATTACTACCCGGTCTGGTCCGAATGCCACAGCGGTAGCTGTTGCAGGTGTTTCGGGACAGCTTTGGTCCCCGAGCACCTTTGGATCAATCATCGTTGTTGATGACATGTTGCTTAAACACCCAAACCCATACTCGCAAAGCGTTGGCCGAGTCCAACGCTTGGTAGTGGCGGCATCAATAGATGGAAGGTACAACTATGTCACCCTCTCTGCTATATTCACAAGCAACGAGCACAATAGCAGCACGTTACAGGCACAAGGAACATTCGATAGGGCTTCCAGGGAACCTAGCGAGCTCTCTATCGTAGGAGGTACAGGAAAGTTTAAGCGCGCTAGTGGAATTGCTATTGTTCAAACATATGACTTCTTACTTTTTCAAGCCATCTTCAAGTTTTCACTTCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

189

Amino Acids

20.73

Weight (kDa)

9.64

Isoelectric Point (pI)

36.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 49 - 175 1.7e-30 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 194
Acc36I ACCTGC 1 cut(s) 194
AccB7I CCANNNNNTGG 1 cut(s) 319
AccI GTMKAC 1 cut(s) 26
AccII CGCG 1 cut(s) 489
AciI CCGC 2 cut(s) 191, 329
AclWI GGATC 1 cut(s) 250
AcoI YGGCCR 1 cut(s) 304
AcsI RAATTY 1 cut(s) 136
AcuI CTGAAG 1 cut(s) 167
AfaI GTAC 3 cut(s) 152, 350, 472
AfiI CCNNNNNNNGG 1 cut(s) 319
AflIII ACRYGT 1 cut(s) 264
AgsI TTSAA 3 cut(s) 512, 536, 547
AjnI CCWGG 1 cut(s) 437
AluBI AGCT 5 cut(s) 104, 145, 197, 220, 454
AluI AGCT 5 cut(s) 104, 145, 197, 220, 454
Alw21I GWGCWC 3 cut(s) 236, 393, 456
AlwI GGATC 1 cut(s) 250
Ama87I CYCGRG 1 cut(s) 229
AoxI GGCC 1 cut(s) 304
ApeKI GCWGC 2 cut(s) 104, 399
ApoI RAATTY 1 cut(s) 136
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
AspLEI GCGC 2 cut(s) 489, 491
AspS9I GGNCC 2 cut(s) 176, 225
AsuC2I CCSGG 2 cut(s) 124, 169
AsuHPI GGTGA 1 cut(s) 352
AvaI CYCGRG 1 cut(s) 229
AvaII GGWCC 2 cut(s) 176, 225
BanII GRGCYC 1 cut(s) 456
Bbv12I GWGCWC 3 cut(s) 236, 393, 456
BbvI GCAGC 2 cut(s) 91, 411
BccI CCATC 2 cut(s) 335, 548
BcgI CGANNNNNNTGC 2 cut(s) 192, 226
BciT130I CCWGG 1 cut(s) 439
BcnI CCSGG 2 cut(s) 124, 169
BfaI CTAG 3 cut(s) 59, 447, 492
BfuAI ACCTGC 1 cut(s) 194
BisI GCNGC 3 cut(s) 105, 330, 400
BlsI GCNGC 3 cut(s) 106, 331, 401
BmcAI AGTACT 1 cut(s) 152
Bme1390I CCNGG 3 cut(s) 124, 169, 439
Bme18I GGWCC 2 cut(s) 176, 225
BmeT110I CYCGRG 1 cut(s) 229
BmgT120I GGNCC 2 cut(s) 176, 225
BmiI GGNNCC 2 cut(s) 227, 444
BmrFI CCNGG 3 cut(s) 124, 169, 439
BmsI GCATC 1 cut(s) 341
BpuMI CCSGG 2 cut(s) 124, 169
BsaJI CCNNGG 2 cut(s) 20, 438
Bsc4I CCNNNNNNNGG 1 cut(s) 319
Bse1I ACTGG 1 cut(s) 54
BseBI CCWGG 1 cut(s) 439
BseDI CCNNGG 2 cut(s) 20, 438
BseLI CCNNNNNNNGG 1 cut(s) 319
BseNI ACTGG 1 cut(s) 54
BsePI GCGCGC 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 91, 411
BsgI GTGCAG 1 cut(s) 90
Bsh1236I CGCG 1 cut(s) 489
BshFI GGCC 1 cut(s) 306
BsiHKAI GWGCWC 3 cut(s) 236, 393, 456
BsiHKCI CYCGRG 1 cut(s) 229
BsiSI CCGG 2 cut(s) 124, 169
BslFI GGGAC 3 cut(s) 106, 211, 228
BslI CCNNNNNNNGG 1 cut(s) 319
BsmFI GGGAC 3 cut(s) 106, 211, 228
BsmI GAATGC 1 cut(s) 187
BsnI GGCC 1 cut(s) 306
BsoBI CYCGRG 1 cut(s) 229
Bsp1286I GDGCHC 3 cut(s) 236, 393, 456
Bsp143I GATC 1 cut(s) 242
BspACI CCGC 2 cut(s) 191, 329
BspANI GGCC 1 cut(s) 306
BspFNI CGCG 1 cut(s) 489
BspHI TCATGA 1 cut(s) 81
BspLI GGNNCC 2 cut(s) 227, 444
BspMI ACCTGC 1 cut(s) 194
BspPI GGATC 1 cut(s) 250
BsrI ACTGG 1 cut(s) 54
BssECI CCNNGG 2 cut(s) 20, 438
BssHII GCGCGC 1 cut(s) 487
BssMI GATC 1 cut(s) 242
BssT1I CCWWGG 1 cut(s) 20
Bst2UI CCWGG 1 cut(s) 439
BstC8I GCNNGC 2 cut(s) 452, 489
BstFNI CGCG 1 cut(s) 489
BstHHI GCGC 2 cut(s) 489, 491
BstKTI GATC 1 cut(s) 245
BstMBI GATC 1 cut(s) 242
BstNI CCWGG 1 cut(s) 439
BstNSI RCATGY 1 cut(s) 268
BstSCI CCNGG 3 cut(s) 122, 167, 437
BstUI CGCG 1 cut(s) 489
BstV1I GCAGC 2 cut(s) 91, 411
BsuRI GGCC 1 cut(s) 306
BveI ACCTGC 1 cut(s) 194
Cac8I GCNNGC 2 cut(s) 452, 489
CciI TCATGA 1 cut(s) 81
CfoI GCGC 2 cut(s) 489, 491
Cfr13I GGNCC 2 cut(s) 176, 225
Csp6I GTAC 3 cut(s) 151, 349, 471
CviAII CATG 2 cut(s) 82, 265
CviJI RGCY 9 cut(s) 5, 104, 145, 197, 220, 306, 434, 454, 539
CviKI_1 RGCY 9 cut(s) 5, 104, 145, 197, 220, 306, 434, 454, 539
CviQI GTAC 3 cut(s) 151, 349, 471
DpnI GATC 1 cut(s) 244
DpnII GATC 1 cut(s) 242
EaeI YGGCCR 1 cut(s) 304
Ecl136II GAGCTC 1 cut(s) 454
Eco130I CCWWGG 1 cut(s) 20
Eco24I GRGCYC 1 cut(s) 456
Eco47I GGWCC 2 cut(s) 176, 225
Eco53kI GAGCTC 1 cut(s) 454
Eco57I CTGAAG 1 cut(s) 167
Eco88I CYCGRG 1 cut(s) 229
EcoICRI GAGCTC 1 cut(s) 454
EcoRII CCWGG 1 cut(s) 437
EcoT14I CCWWGG 1 cut(s) 20
EcoT38I GRGCYC 1 cut(s) 456
ErhI CCWWGG 1 cut(s) 20
FaeI CATG 2 cut(s) 85, 268
FaqI GGGAC 3 cut(s) 106, 211, 228
FatI CATG 2 cut(s) 81, 264
FauNDI CATATG 1 cut(s) 517
FblI GTMKAC 1 cut(s) 26
Fnu4HI GCNGC 3 cut(s) 105, 330, 400
FriOI GRGCYC 1 cut(s) 456
Fsp4HI GCNGC 3 cut(s) 105, 330, 400
FspBI CTAG 3 cut(s) 59, 447, 492
GlaI GCGC 2 cut(s) 488, 490
GluI GCNGC 3 cut(s) 105, 330, 400
HaeIII GGCC 1 cut(s) 306
HapII CCGG 2 cut(s) 124, 169
HhaI GCGC 2 cut(s) 489, 491
Hin1II CATG 2 cut(s) 85, 268
Hin6I GCGC 2 cut(s) 487, 489
HinP1I GCGC 2 cut(s) 487, 489
HincII GTYRAC 1 cut(s) 27
HindII GTYRAC 1 cut(s) 27
HinfI GANTC 1 cut(s) 309
HpaII CCGG 2 cut(s) 124, 169
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 27
Hpy188I TCNGA 1 cut(s) 180
Hpy188III TCNNGA 3 cut(s) 48, 82, 213
Hpy8I GTNNAC 1 cut(s) 27
HpyAV CCTTC 1 cut(s) 339
HpyCH4IV ACGT 1 cut(s) 404
HpyCH4V TGCA 2 cut(s) 107, 203
HpySE526I ACGT 1 cut(s) 404
Hsp92II CATG 2 cut(s) 85, 268
HspAI GCGC 2 cut(s) 487, 489
Kzo9I GATC 1 cut(s) 242
LpnPI CCDG 9 cut(s) 67, 137, 159, 182, 189, 395, 424, 451, 459
Lsp1109I GCAGC 2 cut(s) 91, 411
LweI GCATC 1 cut(s) 341
MaeI CTAG 3 cut(s) 59, 447, 492
MaeII ACGT 1 cut(s) 404
MaeIII GTNAC 2 cut(s) 358, 405
MalI GATC 1 cut(s) 244
MboI GATC 1 cut(s) 242
MboII GAAGA 1 cut(s) 535
MhlI GDGCHC 3 cut(s) 236, 393, 456
MluCI AATT 3 cut(s) 10, 136, 498
MlyI GAGTC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 4 cut(s) 80, 126, 374, 461
MseI TTAA 2 cut(s) 273, 483
MspA1I CMGCKG 1 cut(s) 191
MspI CCGG 2 cut(s) 124, 169
MspR9I CCNGG 3 cut(s) 124, 169, 439
Mva1269I GAATGC 1 cut(s) 187
MvaI CCWGG 1 cut(s) 439
MvnI CGCG 1 cut(s) 489
NciI CCSGG 2 cut(s) 124, 169
NdeI CATATG 1 cut(s) 517
NdeII GATC 1 cut(s) 242
NlaIII CATG 2 cut(s) 85, 268
NlaIV GGNNCC 2 cut(s) 227, 444
NmeAIII GCCGAG 1 cut(s) 332
NmuCI GTSAC 1 cut(s) 358
NspI RCATGY 1 cut(s) 268
PagI TCATGA 1 cut(s) 81
PaqCI CACCTGC 1 cut(s) 194
PauI GCGCGC 1 cut(s) 487
PciI ACATGT 1 cut(s) 264
PctI GAATGC 1 cut(s) 187
PflMI CCANNNNNTGG 1 cut(s) 319
PkrI GCNGC 3 cut(s) 106, 331, 401
PleI GAGTC 1 cut(s) 317
PpsI GAGTC 1 cut(s) 317
PscI ACATGT 1 cut(s) 264
Psp124BI GAGCTC 1 cut(s) 456
Psp6I CCWGG 1 cut(s) 437
PspGI CCWGG 1 cut(s) 437
PspN4I GGNNCC 2 cut(s) 227, 444
PspPI GGNCC 2 cut(s) 176, 225
PteI GCGCGC 1 cut(s) 487
RsaI GTAC 3 cut(s) 152, 350, 472
RsaNI GTAC 3 cut(s) 151, 349, 471
SacI GAGCTC 1 cut(s) 456
SalI GTCGAC 1 cut(s) 25
SaqAI TTAA 2 cut(s) 273, 483
SatI GCNGC 3 cut(s) 105, 330, 400
Sau3AI GATC 1 cut(s) 242
Sau96I GGNCC 2 cut(s) 176, 225
ScaI AGTACT 1 cut(s) 152
SchI GAGTC 1 cut(s) 318
ScrFI CCNGG 3 cut(s) 124, 169, 439
SduI GDGCHC 3 cut(s) 236, 393, 456
SfaNI GCATC 1 cut(s) 341
SinI GGWCC 2 cut(s) 176, 225
Sse9I AATT 3 cut(s) 10, 136, 498
SsiI CCGC 2 cut(s) 191, 329
SspMI CTAG 3 cut(s) 59, 447, 492
SstI GAGCTC 1 cut(s) 456
StyD4I CCNGG 3 cut(s) 122, 167, 437
StyI CCWWGG 1 cut(s) 20
TaiI ACGT 1 cut(s) 407
TaqI TCGA 5 cut(s) 26, 49, 99, 141, 426
TasI AATT 3 cut(s) 10, 136, 498
TatI WGTACW 1 cut(s) 150
TauI GCSGC 1 cut(s) 332
Tru1I TTAA 2 cut(s) 273, 483
Tru9I TTAA 2 cut(s) 273, 483
TseFI GTSAC 1 cut(s) 358
TseI GCWGC 2 cut(s) 104, 399
Tsp45I GTSAC 1 cut(s) 358
Van91I CCANNNNNTGG 1 cut(s) 319
VpaK11BI GGWCC 2 cut(s) 176, 225
XapI RAATTY 1 cut(s) 136
XceI RCATGY 1 cut(s) 268
XmiI GTMKAC 1 cut(s) 26
XspI CTAG 3 cut(s) 59, 447, 492
ZrmI AGTACT 1 cut(s) 152
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.