Rorug02G0477700

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0008878
Physical Location & Seq
Reverse (-)
60908173 .. 60908652
480 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0477700.1

Sequence Viewer

Length: 480 bp
ATGGATGTGGATGTCCTCAGCTGGAATTGCCGAGGTATATGTAATGATGCCACGACACGGGAATTGAAGGACTTGATTACTCAAAATCGGCCTCAGATAGTGTTTTTATGTGAAACGAAAATCAGCCGTCAGGAGGAGTTTCACAAACTGCATCGTGCTCTAGGGTTCGCTCATGCTGAATCGGTTCTGAGCGAGGGACAGTCTGGTGGATTGGCGCTCTTCTGGAATGATGAGATTAACCTGCAGATGAGAACAACATCGGCCCATCATATGGACGCTGTGATTGTGGGGGATTCTGGTGCTCTTTTATGGAGAATGACAGGTTTTTATGGGTATGCAAGAACTGGGGATCATGATTGCTCCTGGCAGCTCCTCCGGGACCTTTCAGATCTCGATTCGCTACCATGGATCATGGTCGGGGATTTTAATGAGATCTTGAATAATGGAGAGAAGATTGACGGGCCGCTTTTTCCAGGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

159

Amino Acids

17.94

Weight (kDa)

4.86

Isoelectric Point (pI)

30.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Exo_endo_phos PF03372 6 - 150 2.1e-13 Endonuclease/Exonuclease/phosphatase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 249
AccB7I CCANNNNNTGG 1 cut(s) 271
AciI CCGC 1 cut(s) 464
AclWI GGATC 2 cut(s) 357, 416
AfiI CCNNNNNNNGG 3 cut(s) 57, 133, 271
AgsI TTSAA 2 cut(s) 67, 439
AjnI CCWGG 2 cut(s) 362, 472
AluBI AGCT 2 cut(s) 21, 370
AluI AGCT 2 cut(s) 21, 370
Alw21I GWGCWC 2 cut(s) 160, 304
AlwI GGATC 2 cut(s) 357, 416
AoxI GGCC 3 cut(s) 89, 261, 461
ApeKI GCWGC 1 cut(s) 367
Asp700I GAANNNNTTC 1 cut(s) 183
AspLEI GCGC 1 cut(s) 217
AspS9I GGNCC 3 cut(s) 262, 379, 461
AsuC2I CCSGG 1 cut(s) 377
AvaII GGWCC 1 cut(s) 379
Bbv12I GWGCWC 2 cut(s) 160, 304
BbvCI CCTCAGC 1 cut(s) 17
BbvI GCAGC 1 cut(s) 379
BccI CCATC 1 cut(s) 273
BceAI ACGGC 1 cut(s) 111
BciT130I CCWGG 2 cut(s) 364, 474
BcnI CCSGG 1 cut(s) 377
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 242
BfoI RGCGCY 1 cut(s) 218
BfuAI ACCTGC 1 cut(s) 249
BglII AGATCT 2 cut(s) 388, 432
BisI GCNGC 2 cut(s) 368, 464
BlsI GCNGC 2 cut(s) 369, 465
Bme1390I CCNGG 3 cut(s) 364, 377, 474
Bme18I GGWCC 1 cut(s) 379
BmgT120I GGNCC 3 cut(s) 262, 379, 461
BmiI GGNNCC 1 cut(s) 380
BmrFI CCNGG 3 cut(s) 364, 377, 474
BmrI ACTGGG 1 cut(s) 354
BmsI GCATC 2 cut(s) 37, 160
BmuI ACTGGG 1 cut(s) 354
Bpu10I CCTNAGC 1 cut(s) 17
BpuMI CCSGG 1 cut(s) 377
BsaJI CCNNGG 2 cut(s) 31, 404
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 133, 271
Bse1I ACTGG 1 cut(s) 349
BseBI CCWGG 2 cut(s) 364, 474
BseDI CCNNGG 2 cut(s) 31, 404
BseGI GGATG 2 cut(s) 10, 16
BseLI CCNNNNNNNGG 3 cut(s) 57, 133, 271
BseMII CTCAG 3 cut(s) 31, 107, 179
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 2 cut(s) 149, 362
BseXI GCAGC 1 cut(s) 379
BshFI GGCC 3 cut(s) 91, 263, 463
BsiHKAI GWGCWC 2 cut(s) 160, 304
BsiSI CCGG 1 cut(s) 376
BslFI GGGAC 2 cut(s) 210, 392
BslI CCNNNNNNNGG 3 cut(s) 57, 133, 271
BsmFI GGGAC 2 cut(s) 210, 392
BsnI GGCC 3 cut(s) 91, 263, 463
Bsp1286I GDGCHC 2 cut(s) 160, 304
Bsp143I GATC 4 cut(s) 349, 388, 408, 432
Bsp19I CCATGG 1 cut(s) 404
BspACI CCGC 1 cut(s) 464
BspANI GGCC 3 cut(s) 91, 263, 463
BspCNI CTCAG 3 cut(s) 30, 106, 180
BspHI TCATGA 1 cut(s) 352
BspLI GGNNCC 1 cut(s) 380
BspMAI CTGCAG 1 cut(s) 246
BspMI ACCTGC 1 cut(s) 249
BspPI GGATC 2 cut(s) 357, 416
BspQI GCTCTTC 1 cut(s) 224
BsrI ACTGG 1 cut(s) 349
BssECI CCNNGG 2 cut(s) 31, 404
BssMI GATC 4 cut(s) 349, 388, 408, 432
BssT1I CCWWGG 1 cut(s) 404
Bst2UI CCWGG 2 cut(s) 364, 474
Bst4CI ACNGT 1 cut(s) 201
Bst6I CTCTTC 1 cut(s) 224
BstDEI CTNAG 3 cut(s) 17, 93, 188
BstDSI CCRYGG 1 cut(s) 404
BstF5I GGATG 2 cut(s) 10, 16
BstH2I RGCGCY 1 cut(s) 218
BstHHI GCGC 1 cut(s) 217
BstKTI GATC 4 cut(s) 352, 391, 411, 435
BstMBI GATC 4 cut(s) 349, 388, 408, 432
BstMWI GCNNNNNNNGC 1 cut(s) 27
BstNI CCWGG 2 cut(s) 364, 474
BstSCI CCNGG 3 cut(s) 362, 375, 472
BstSFI CTRYAG 1 cut(s) 242
BstV1I GCAGC 1 cut(s) 379
BstX2I RGATCY 2 cut(s) 388, 432
BstYI RGATCY 2 cut(s) 388, 432
BsuRI GGCC 3 cut(s) 91, 263, 463
BtgI CCRYGG 1 cut(s) 404
BtsCI GGATG 2 cut(s) 10, 16
BveI ACCTGC 1 cut(s) 249
CciI TCATGA 1 cut(s) 352
CfoI GCGC 1 cut(s) 217
Cfr13I GGNCC 3 cut(s) 262, 379, 461
CseI GACGC 1 cut(s) 284
CviAII CATG 4 cut(s) 173, 353, 405, 412
CviJI RGCY 6 cut(s) 21, 91, 126, 263, 370, 463
CviKI_1 RGCY 6 cut(s) 21, 91, 126, 263, 370, 463
DdeI CTNAG 3 cut(s) 17, 93, 188
DpnI GATC 4 cut(s) 351, 390, 410, 434
DpnII GATC 4 cut(s) 349, 388, 408, 432
Eam1104I CTCTTC 1 cut(s) 224
EarI CTCTTC 1 cut(s) 224
Eco130I CCWWGG 1 cut(s) 404
Eco47I GGWCC 1 cut(s) 379
EcoO109I RGGNCCY 1 cut(s) 379
EcoRII CCWGG 2 cut(s) 362, 472
EcoT14I CCWWGG 1 cut(s) 404
ErhI CCWWGG 1 cut(s) 404
FaeI CATG 4 cut(s) 176, 356, 408, 415
FaqI GGGAC 2 cut(s) 210, 392
FatI CATG 4 cut(s) 172, 352, 404, 411
FauNDI CATATG 1 cut(s) 270
Fnu4HI GCNGC 2 cut(s) 368, 464
FokI GGATG 2 cut(s) 17, 23
Fsp4HI GCNGC 2 cut(s) 368, 464
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 216
GluI GCNGC 2 cut(s) 368, 464
HaeII RGCGCY 1 cut(s) 218
HaeIII GGCC 3 cut(s) 91, 263, 463
HapII CCGG 1 cut(s) 376
HgaI GACGC 1 cut(s) 284
HhaI GCGC 1 cut(s) 217
Hin1II CATG 4 cut(s) 176, 356, 408, 415
Hin6I GCGC 1 cut(s) 215
HinP1I GCGC 1 cut(s) 215
HinfI GANTC 3 cut(s) 179, 293, 395
HpaII CCGG 1 cut(s) 376
Hpy188I TCNGA 3 cut(s) 96, 189, 388
Hpy188III TCNNGA 5 cut(s) 131, 223, 353, 392, 436
HpyAV CCTTC 1 cut(s) 61
HpyCH4III ACNGT 1 cut(s) 201
HpyCH4V TGCA 3 cut(s) 151, 244, 338
HpyF10VI GCNNNNNNNGC 1 cut(s) 27
HpyF3I CTNAG 3 cut(s) 17, 93, 188
Hsp92II CATG 4 cut(s) 176, 356, 408, 415
HspAI GCGC 1 cut(s) 215
Kzo9I GATC 4 cut(s) 349, 388, 408, 432
LguI GCTCTTC 1 cut(s) 224
LmnI GCTCC 2 cut(s) 365, 375
Lsp1109I GCAGC 1 cut(s) 379
LweI GCATC 2 cut(s) 37, 160
MaeI CTAG 1 cut(s) 161
MalI GATC 4 cut(s) 351, 390, 410, 434
MboI GATC 4 cut(s) 349, 388, 408, 432
MboII GAAGA 2 cut(s) 211, 463
MflI RGATCY 2 cut(s) 388, 432
MhlI GDGCHC 2 cut(s) 160, 304
MluCI AATT 2 cut(s) 25, 62
MnlI CCTC 6 cut(s) 26, 26, 102, 127, 187, 383
MroXI GAANNNNTTC 1 cut(s) 183
MseI TTAA 2 cut(s) 237, 426
MspA1I CMGCKG 1 cut(s) 21
MspI CCGG 1 cut(s) 376
MspR9I CCNGG 3 cut(s) 364, 377, 474
MvaI CCWGG 2 cut(s) 364, 474
MwoI GCNNNNNNNGC 1 cut(s) 27
NciI CCSGG 1 cut(s) 377
NcoI CCATGG 1 cut(s) 404
NdeI CATATG 1 cut(s) 270
NdeII GATC 4 cut(s) 349, 388, 408, 432
NlaIII CATG 4 cut(s) 176, 356, 408, 415
NlaIV GGNNCC 1 cut(s) 380
NmeAIII GCCGAG 1 cut(s) 56
PagI TCATGA 1 cut(s) 352
PciSI GCTCTTC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 183
PfeI GAWTC 3 cut(s) 179, 293, 395
PflMI CCANNNNNTGG 1 cut(s) 271
PfoI TCCNGGA 2 cut(s) 375, 472
PkrI GCNGC 2 cut(s) 369, 465
PpuMI RGGWCCY 1 cut(s) 379
Psp5II RGGWCCY 1 cut(s) 379
Psp6I CCWGG 2 cut(s) 362, 472
PspGI CCWGG 2 cut(s) 362, 472
PspN4I GGNNCC 1 cut(s) 380
PspPI GGNCC 3 cut(s) 262, 379, 461
PspPPI RGGWCCY 1 cut(s) 379
PstI CTGCAG 1 cut(s) 246
PsuI RGATCY 2 cut(s) 388, 432
PvuII CAGCTG 1 cut(s) 21
SapI GCTCTTC 1 cut(s) 224
SaqAI TTAA 2 cut(s) 237, 426
SatI GCNGC 2 cut(s) 368, 464
Sau3AI GATC 4 cut(s) 349, 388, 408, 432
Sau96I GGNCC 3 cut(s) 262, 379, 461
ScrFI CCNGG 3 cut(s) 364, 377, 474
SduI GDGCHC 2 cut(s) 160, 304
SetI ASST 6 cut(s) 23, 37, 243, 325, 372, 384
SfaNI GCATC 2 cut(s) 37, 160
SfcI CTRYAG 1 cut(s) 242
SinI GGWCC 1 cut(s) 379
Sse9I AATT 2 cut(s) 25, 62
SsiI CCGC 1 cut(s) 464
SspMI CTAG 1 cut(s) 161
StyD4I CCNGG 3 cut(s) 362, 375, 472
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 1 cut(s) 201
TaqI TCGA 1 cut(s) 393
TasI AATT 2 cut(s) 25, 62
TauI GCSGC 1 cut(s) 466
TfiI GAWTC 3 cut(s) 179, 293, 395
Tru1I TTAA 2 cut(s) 237, 426
Tru9I TTAA 2 cut(s) 237, 426
TseI GCWGC 1 cut(s) 367
Van91I CCANNNNNTGG 1 cut(s) 271
VpaK11BI GGWCC 1 cut(s) 379
XmnI GAANNNNTTC 1 cut(s) 183
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.