Rorug05G0220600

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008878
Physical Location & Seq
Reverse (-)
22712339 .. 22713801
1463 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0220600.1

Sequence Viewer

Length: 543 bp
ATGCAGGAGAACGGTGATCTGGAGATGAACAGTGATCTGGAGATGGAAGAATACCGTGAAGACCTGATCACCAAGAAAGGGTCTGATGCTCGGGTTATGGCAGAAGCTCGTTGGAGAGCAAATCCGGATGGAAACGATCTGGAGATCAAAGTTTTACTTTGGGTGCCCAAAGTAAATCGTTGGGTCTGGAGCTGGGAATGGAGGCCTGGCAGTGATGAAAACTGCTACAATGAGTCCTATCCAATTCAAGGTTCATATTGGGGTACTGGTTCGAACACCAAAATCATGGATATTATAGATGACACGTTACAGGATTTGAAAGTTAAGGTTACATTTTTGAATATCACACAATTGTCTGAGTACAGAAAAGATGCACACACATCAGTTTATGGGGAACGCAAGGGCAAGCTCCTGACGAAGGAGCAAAGATCTGATCCAAAAAACTTTGCAGATTGCATTCACTGGTGCTTACCTGGAGTTCCAGATACATGGAACGAAATTTTGTACGCATATTTGTTAAAGAGTCATCAAAATTTTTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

180

Amino Acids

21.14

Weight (kDa)

4.96

Isoelectric Point (pI)

23.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PC-Esterase PF13839 64 - 170 6.7e-31 GDSL/SGNH-like Acyl-Esterase family found in Pmr5 and Cas1p
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 163
AccIII TCCGGA 1 cut(s) 124
AclWI GGATC 1 cut(s) 428
AcsI RAATTY 2 cut(s) 498, 532
AfaI GTAC 3 cut(s) 265, 362, 506
AfiI CCNNNNNNNGG 3 cut(s) 78, 248, 418
AflIII ACRYGT 1 cut(s) 303
AgsI TTSAA 3 cut(s) 248, 319, 340
AjnI CCWGG 2 cut(s) 205, 472
AjuI GAANNNNNNNTTGG 2 cut(s) 235, 267
AluBI AGCT 3 cut(s) 107, 192, 409
AluI AGCT 3 cut(s) 107, 192, 409
AlwI GGATC 1 cut(s) 428
Ama87I CYCGRG 1 cut(s) 90
Aor13HI TCCGGA 1 cut(s) 124
AoxI GGCC 1 cut(s) 203
ApoI RAATTY 2 cut(s) 498, 532
AsuHPI GGTGA 2 cut(s) 26, 61
AsuII TTCGAA 1 cut(s) 272
AvaI CYCGRG 1 cut(s) 90
BaeGI GKGCMC 1 cut(s) 168
BanI GGYRCC 1 cut(s) 163
BbsI GAAGAC 1 cut(s) 66
BccI CCATC 2 cut(s) 37, 122
BciT130I CCWGG 2 cut(s) 207, 474
BclI TGATCA 1 cut(s) 66
BglII AGATCT 1 cut(s) 428
Bme1390I CCNGG 2 cut(s) 207, 474
BmeT110I CYCGRG 1 cut(s) 90
BmiI GGNNCC 1 cut(s) 165
BmrFI CCNGG 2 cut(s) 207, 474
BmsI GCATC 2 cut(s) 76, 361
BpiI GAAGAC 1 cut(s) 66
BpmI CTGGAG 5 cut(s) 41, 59, 161, 208, 495
Bpu14I TTCGAA 1 cut(s) 272
BsaWI WCCGGW 1 cut(s) 124
Bsc4I CCNNNNNNNGG 3 cut(s) 78, 248, 418
Bse1I ACTGG 2 cut(s) 271, 467
BseAI TCCGGA 1 cut(s) 124
BseBI CCWGG 2 cut(s) 207, 474
BseGI GGATG 1 cut(s) 133
BseLI CCNNNNNNNGG 3 cut(s) 78, 248, 418
BseMII CTCAG 1 cut(s) 348
BseNI ACTGG 2 cut(s) 271, 467
BseSI GKGCMC 1 cut(s) 168
BseYI CCCAGC 1 cut(s) 192
BshFI GGCC 1 cut(s) 205
BshNI GGYRCC 1 cut(s) 163
BsiHKCI CYCGRG 1 cut(s) 90
BsiSI CCGG 1 cut(s) 125
BslI CCNNNNNNNGG 3 cut(s) 78, 248, 418
BsmI GAATGC 1 cut(s) 456
BsnI GGCC 1 cut(s) 205
BsoBI CYCGRG 1 cut(s) 90
Bsp119I TTCGAA 1 cut(s) 272
Bsp1286I GDGCHC 1 cut(s) 168
Bsp13I TCCGGA 1 cut(s) 124
Bsp143I GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
BspANI GGCC 1 cut(s) 205
BspCNI CTCAG 1 cut(s) 349
BspEI TCCGGA 1 cut(s) 124
BspLI GGNNCC 1 cut(s) 165
BspPI GGATC 1 cut(s) 428
BspT104I TTCGAA 1 cut(s) 272
BspT107I GGYRCC 1 cut(s) 163
BsrI ACTGG 2 cut(s) 271, 467
BssMI GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
Bst2UI CCWGG 2 cut(s) 207, 474
Bst4CI ACNGT 3 cut(s) 14, 32, 56
BstBI TTCGAA 1 cut(s) 272
BstC8I GCNNGC 1 cut(s) 407
BstDEI CTNAG 1 cut(s) 357
BstENI CCTNNNNNAGG 1 cut(s) 416
BstF5I GGATG 1 cut(s) 133
BstKTI GATC 7 cut(s) 19, 37, 69, 139, 147, 431, 436
BstMBI GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
BstNI CCWGG 2 cut(s) 207, 474
BstSCI CCNGG 2 cut(s) 205, 472
BstSLI GKGCMC 1 cut(s) 168
BstV2I GAAGAC 1 cut(s) 66
BstX2I RGATCY 1 cut(s) 428
BstXI CCANNNNNNTGG 2 cut(s) 286, 489
BstYI RGATCY 1 cut(s) 428
BsuRI GGCC 1 cut(s) 205
BtsCI GGATG 1 cut(s) 133
BtsI GCAGTG 1 cut(s) 217
BtsIMutI CAGTG 3 cut(s) 37, 217, 460
Cac8I GCNNGC 1 cut(s) 407
Csp6I GTAC 3 cut(s) 264, 361, 505
CviAII CATG 2 cut(s) 286, 489
CviJI RGCY 4 cut(s) 107, 192, 205, 409
CviKI_1 RGCY 4 cut(s) 107, 192, 205, 409
CviQI GTAC 3 cut(s) 264, 361, 505
DdeI CTNAG 1 cut(s) 357
DpnI GATC 7 cut(s) 18, 36, 68, 138, 146, 430, 435
DpnII GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
Eco147I AGGCCT 1 cut(s) 205
Eco88I CYCGRG 1 cut(s) 90
EcoNI CCTNNNNNAGG 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 205, 472
FaeI CATG 2 cut(s) 289, 492
FaiI YATR 7 cut(s) 98, 256, 287, 296, 390, 490, 511
FalI AAGNNNNNCTT 2 cut(s) 141, 173
FatI CATG 2 cut(s) 285, 488
FbaI TGATCA 1 cut(s) 66
FokI GGATG 1 cut(s) 140
GsaI CCCAGC 1 cut(s) 196
GsuI CTGGAG 5 cut(s) 41, 59, 161, 208, 495
HaeIII GGCC 1 cut(s) 205
HapII CCGG 1 cut(s) 125
Hin1II CATG 2 cut(s) 289, 492
HinfI GANTC 2 cut(s) 233, 523
HpaII CCGG 1 cut(s) 125
HphI GGTGA 2 cut(s) 26, 61
Hpy188I TCNGA 3 cut(s) 85, 358, 433
Hpy188III TCNNGA 7 cut(s) 20, 38, 125, 140, 187, 412, 482
HpyAV CCTTC 1 cut(s) 412
HpyCH4III ACNGT 3 cut(s) 14, 32, 56
HpyCH4IV ACGT 1 cut(s) 305
HpyCH4V TGCA 4 cut(s) 4, 374, 449, 456
HpyF3I CTNAG 1 cut(s) 357
HpySE526I ACGT 1 cut(s) 305
Hsp92II CATG 2 cut(s) 289, 492
Kpn2I TCCGGA 1 cut(s) 124
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
LmnI GCTCC 3 cut(s) 189, 414, 421
LweI GCATC 2 cut(s) 76, 361
MaeII ACGT 1 cut(s) 305
MaeIII GTNAC 2 cut(s) 306, 328
MalI GATC 7 cut(s) 18, 36, 68, 138, 146, 430, 435
MboI GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
MboII GAAGA 2 cut(s) 59, 71
MfeI CAATTG 1 cut(s) 350
MflI RGATCY 1 cut(s) 428
MhlI GDGCHC 1 cut(s) 168
MluCI AATT 4 cut(s) 243, 350, 498, 532
MlyI GAGTC 2 cut(s) 242, 532
MmeI TCCRAC 1 cut(s) 92
MnlI CCTC 1 cut(s) 195
MroI TCCGGA 1 cut(s) 124
MseI TTAA 2 cut(s) 324, 518
MspI CCGG 1 cut(s) 125
MspR9I CCNGG 2 cut(s) 207, 474
MunI CAATTG 1 cut(s) 350
Mva1269I GAATGC 1 cut(s) 456
MvaI CCWGG 2 cut(s) 207, 474
NdeII GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
NlaIII CATG 2 cut(s) 289, 492
NlaIV GGNNCC 1 cut(s) 165
NspV TTCGAA 1 cut(s) 272
PceI AGGCCT 1 cut(s) 205
PctI GAATGC 1 cut(s) 456
PleI GAGTC 2 cut(s) 241, 531
PpsI GAGTC 2 cut(s) 241, 531
Psp6I CCWGG 2 cut(s) 205, 472
PspFI CCCAGC 1 cut(s) 192
PspGI CCWGG 2 cut(s) 205, 472
PspN4I GGNNCC 1 cut(s) 165
PsuI RGATCY 1 cut(s) 428
RsaI GTAC 3 cut(s) 265, 362, 506
RsaNI GTAC 3 cut(s) 264, 361, 505
SaqAI TTAA 2 cut(s) 324, 518
Sau3AI GATC 7 cut(s) 16, 34, 66, 136, 144, 428, 433
SchI GAGTC 2 cut(s) 242, 532
ScrFI CCNGG 2 cut(s) 207, 474
SduI GDGCHC 1 cut(s) 168
SetI ASST 8 cut(s) 66, 109, 194, 253, 308, 330, 411, 475
SfaNI GCATC 2 cut(s) 76, 361
SfuI TTCGAA 1 cut(s) 272
Sse9I AATT 4 cut(s) 243, 350, 498, 532
SseBI AGGCCT 1 cut(s) 205
StuI AGGCCT 1 cut(s) 205
StyD4I CCNGG 2 cut(s) 205, 472
TaaI ACNGT 3 cut(s) 14, 32, 56
TaiI ACGT 1 cut(s) 308
TaqI TCGA 1 cut(s) 272
TasI AATT 4 cut(s) 243, 350, 498, 532
TatI WGTACW 1 cut(s) 360
Tru1I TTAA 2 cut(s) 324, 518
Tru9I TTAA 2 cut(s) 324, 518
TscAI CASTG 3 cut(s) 37, 217, 467
TspDTI ATGAA 3 cut(s) 41, 231, 243
TspRI CASTG 3 cut(s) 37, 217, 467
XagI CCTNNNNNAGG 1 cut(s) 416
XapI RAATTY 2 cut(s) 498, 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.