FvH4_6g43700

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0008960
Physical Location & Seq
Forward (+)
33758581 .. 33760681
2101 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g43700.t1

Sequence Viewer

Length: 1179 bp
ATGGCTCCCAAGCGCCTCCTTGAAGACCCTCCCGCTGCTTCCTCCATGGAGGAAGACTCCGACGCCGAGACCGTGGATGAAGAAGAGGAGGAGGTACAAAACGACGTCGTTGAGGACGAAGAAGAAAAATCTGGTGATGAGGAGGATGATGAGGATGAAGAAGAAGACGACGATGAGGAGAATGTGACTGCTAATAAGAAGCCTCAATCGGAATCTGCCTCCGACGCCGGCTCCGAGACCGAGTCCGACGCCATGACCCACTCCCCAACCGCTTCCGATTTCACCGTCAAGCCCATTGTCTCCAAGCCCATGAACGACTCCCCCGCCAAGCCCACCAGGAAGACAGCTGCTCCGGCGAAGCGCGCGGCGGAGAGCGATCCCAATCCGAAAGACTCCAAGAAGAAGAAGAAGGGATCCAACGGCAGCGGCGAGGATGAGGAGACCAAGAAGGCTGGCCGGCTGTGGGGCGAGGATGATGAGATTGCGATATTGGAAGGGATGATTGAGTTCAAGGCCAAGAATGGGACCGACCCGTCTAGCAATATGGGCGCGTTTCATGAATTCTTGAGGAGGAGCCTGAAGGCTGACGTCAACAAGAACCAGTTGATGGATAAGGCGAGGAGGCTGAAGAAGAAGTACTACCTCCCCAATGCTGCTGACAAGGGCGAGAATGTGTTTTCGAAAGCGCATGAGCTGAAGTTGGTTGATTTGTCAAACAAGATTTGGGGGGTTGAGGCTAATGGGGGTGGTGATGGTGGGAAGAACAGTAAGAAGAAGCCGAGGAAGAGTGCCAAGGCTGTTGCTGTTGACAACAGCGCTTCTCCGGTCTTGGCATTGCCTGTTTCGTATGTCCCTGCTGAGAAGAGTGCTGAGAAGAAAGTGGAGAAGAAGGCGGAGATGAATAATGTTGCTAATGGCGGGATGGAGGCTGAGTCGGATGACTTGTTTTGGTCCAAGTACCCATTCCTGAGAGATTCTTTGCGGTTTGGAAAGTGGGATGGTTTGGAGTTGGTTTTGATGCATGGAATGCCTTCAATTGGGAGCTCAAAGGCCAAAGAGTTAGATGATAGGTGGAGGAAGTTGCAGGTCTCAGAGATGGAGTTGGTTGCCAAGAAGCTTGCATTAGAGCATGAAATGACTAAGTTTATATTGGATTCGATGAAGTCTCGAAAGGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

393

Amino Acids

43.3

Weight (kDa)

4.94

Isoelectric Point (pI)

52.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 153 - 243 5e-28 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 532
AatII GACGTC 2 cut(s) 108, 591
Acc36I ACCTGC 1 cut(s) 1075
AccB7I CCANNNNNTGG 1 cut(s) 607
AccII CGCG 3 cut(s) 363, 365, 551
AciI CCGC 9 cut(s) 33, 270, 324, 365, 368, 426, 893, 918, 982
AclWI GGATC 3 cut(s) 371, 408, 421
AcoI YGGCCR 1 cut(s) 454
AcsI RAATTY 1 cut(s) 560
AcuI CTGAAG 3 cut(s) 599, 647, 716
AcyI GRCGYC 5 cut(s) 63, 105, 225, 249, 588
AfaI GTAC 3 cut(s) 96, 638, 959
AfeI AGCGCT 1 cut(s) 817
AfiI CCNNNNNNNGG 4 cut(s) 463, 522, 607, 1037
AgsI TTSAA 3 cut(s) 23, 511, 1035
AjnI CCWGG 1 cut(s) 335
AjuI GAANNNNNNNTTGG 2 cut(s) 947, 979
AluBI AGCT 4 cut(s) 347, 694, 1044, 1117
AluI AGCT 4 cut(s) 347, 694, 1044, 1117
Alw21I GWGCWC 1 cut(s) 1046
Alw26I GTCTC 6 cut(s) 62, 230, 304, 434, 1093, 1170
AlwI GGATC 3 cut(s) 371, 408, 421
Aor51HI AGCGCT 1 cut(s) 817
AoxI GGCC 3 cut(s) 454, 513, 1050
ApeKI GCWGC 4 cut(s) 35, 347, 423, 653
ApoI RAATTY 1 cut(s) 560
Asp700I GAANNNNTTC 1 cut(s) 1030
AspLEI GCGC 6 cut(s) 15, 363, 365, 551, 688, 818
AspS9I GGNCC 2 cut(s) 525, 951
AsuHPI GGTGA 3 cut(s) 146, 274, 761
AsuII TTCGAA 1 cut(s) 680
AvaII GGWCC 2 cut(s) 525, 951
BamHI GGATCC 1 cut(s) 413
BanII GRGCYC 1 cut(s) 1046
BarI GAAGNNNNNNTAC 4 cut(s) 620, 623, 652, 655
BbsI GAAGAC 4 cut(s) 30, 60, 171, 347
Bbv12I GWGCWC 1 cut(s) 1046
BbvI GCAGC 4 cut(s) 22, 334, 435, 640
BccI CCATC 5 cut(s) 601, 746, 916, 992, 1090
BceAI ACGGC 1 cut(s) 436
BciT130I CCWGG 1 cut(s) 337
BcoDI GTCTC 6 cut(s) 62, 230, 304, 434, 1093, 1170
BfaI CTAG 1 cut(s) 537
BfoI RGCGCY 2 cut(s) 16, 819
BfuAI ACCTGC 1 cut(s) 1075
BisI GCNGC 6 cut(s) 36, 348, 366, 424, 427, 654
BlsI GCNGC 6 cut(s) 37, 349, 367, 425, 428, 655
BmcAI AGTACT 1 cut(s) 638
Bme1390I CCNGG 1 cut(s) 337
Bme18I GGWCC 2 cut(s) 525, 951
BmgT120I GGNCC 2 cut(s) 525, 951
BmiI GGNNCC 5 cut(s) 6, 232, 415, 526, 575
BmrFI CCNGG 1 cut(s) 337
BmsI GCATC 1 cut(s) 1008
BpiI GAAGAC 4 cut(s) 30, 60, 171, 347
BplI GAGNNNNNCTC 2 cut(s) 41, 73
Bpu14I TTCGAA 1 cut(s) 680
BpuEI CTTGAG 1 cut(s) 586
BsaBI GATNNNNATC 1 cut(s) 381
BsaHI GRCGYC 5 cut(s) 63, 105, 225, 249, 588
BsaI GGTCTC 4 cut(s) 62, 230, 434, 1093
BsaJI CCNNGG 4 cut(s) 45, 72, 779, 792
BsaWI WCCGGW 1 cut(s) 823
BsaXI ACNNNNNCTCC 6 cut(s) 215, 245, 334, 364, 917, 947
Bsc4I CCNNNNNNNGG 4 cut(s) 463, 522, 607, 1037
Bse118I RCCGGY 2 cut(s) 227, 456
Bse1I ACTGG 1 cut(s) 601
Bse3DI GCAATG 1 cut(s) 833
Bse8I GATNNNNATC 1 cut(s) 381
BseBI CCWGG 1 cut(s) 337
BseDI CCNNGG 4 cut(s) 45, 72, 779, 792
BseGI GGATG 9 cut(s) 82, 151, 160, 439, 478, 504, 927, 943, 1003
BseJI GATNNNNATC 1 cut(s) 381
BseLI CCNNNNNNNGG 4 cut(s) 463, 522, 607, 1037
BseMI GCAATG 1 cut(s) 833
BseMII CTCAG 5 cut(s) 849, 861, 921, 959, 1104
BseNI ACTGG 1 cut(s) 601
BsePI GCGCGC 1 cut(s) 361
BseRI GAGGAG 8 cut(s) 101, 104, 155, 191, 452, 583, 586, 634
BseXI GCAGC 4 cut(s) 22, 334, 435, 640
Bsh1236I CGCG 3 cut(s) 363, 365, 551
BshFI GGCC 3 cut(s) 456, 515, 1052
BsiHKAI GWGCWC 1 cut(s) 1046
BsiSI CCGG 4 cut(s) 228, 353, 457, 824
BslFI GGGAC 2 cut(s) 538, 836
BslI CCNNNNNNNGG 4 cut(s) 463, 522, 607, 1037
BsmAI GTCTC 6 cut(s) 62, 230, 304, 434, 1093, 1170
BsmFI GGGAC 2 cut(s) 538, 836
BsmI GAATGC 1 cut(s) 1032
BsnI GGCC 3 cut(s) 456, 515, 1052
Bso31I GGTCTC 4 cut(s) 62, 230, 434, 1093
Bsp119I TTCGAA 1 cut(s) 680
Bsp1286I GDGCHC 1 cut(s) 1046
Bsp143I GATC 2 cut(s) 376, 413
Bsp19I CCATGG 1 cut(s) 45
BspACI CCGC 9 cut(s) 33, 270, 324, 365, 368, 426, 893, 918, 982
BspANI GGCC 3 cut(s) 456, 515, 1052
BspCNI CTCAG 5 cut(s) 850, 862, 922, 960, 1103
BspFNI CGCG 3 cut(s) 363, 365, 551
BspHI TCATGA 1 cut(s) 556
BspLI GGNNCC 5 cut(s) 6, 232, 415, 526, 575
BspMI ACCTGC 1 cut(s) 1075
BspPI GGATC 3 cut(s) 371, 408, 421
BspT104I TTCGAA 1 cut(s) 680
BspTNI GGTCTC 4 cut(s) 62, 230, 434, 1093
BsrDI GCAATG 1 cut(s) 833
BsrFI RCCGGY 2 cut(s) 227, 456
BsrI ACTGG 1 cut(s) 601
BssAI RCCGGY 2 cut(s) 227, 456
BssECI CCNNGG 4 cut(s) 45, 72, 779, 792
BssHII GCGCGC 1 cut(s) 361
BssMI GATC 2 cut(s) 376, 413
BssNI GRCGYC 5 cut(s) 63, 105, 225, 249, 588
BssT1I CCWWGG 2 cut(s) 45, 792
Bst2UI CCWGG 1 cut(s) 337
Bst4CI ACNGT 3 cut(s) 73, 286, 767
Bst6I CTCTTC 3 cut(s) 78, 779, 857
BstACI GRCGYC 5 cut(s) 63, 105, 225, 249, 588
BstAPI GCANNNNNTGC 1 cut(s) 1027
BstBI TTCGAA 1 cut(s) 680
BstC8I GCNNGC 5 cut(s) 229, 363, 454, 458, 1119
BstDEI CTNAG 6 cut(s) 858, 870, 930, 968, 1090, 1140
BstDSI CCRYGG 2 cut(s) 45, 72
BstF5I GGATG 9 cut(s) 82, 151, 160, 439, 478, 504, 927, 943, 1003
BstFNI CGCG 3 cut(s) 363, 365, 551
BstH2I RGCGCY 2 cut(s) 16, 819
BstHHI GCGC 6 cut(s) 15, 363, 365, 551, 688, 818
BstKTI GATC 2 cut(s) 379, 416
BstMAI GTCTC 6 cut(s) 62, 230, 304, 434, 1093, 1170
BstMBI GATC 2 cut(s) 376, 413
BstMWI GCNNNNNNNGC 5 cut(s) 224, 353, 362, 546, 1027
BstNI CCWGG 1 cut(s) 337
BstSCI CCNGG 1 cut(s) 335
BstUI CGCG 3 cut(s) 363, 365, 551
BstV1I GCAGC 4 cut(s) 22, 334, 435, 640
BstV2I GAAGAC 4 cut(s) 30, 60, 171, 347
BstX2I RGATCY 1 cut(s) 413
BstYI RGATCY 1 cut(s) 413
BsuRI GGCC 3 cut(s) 456, 515, 1052
BtgI CCRYGG 2 cut(s) 45, 72
BtsCI GGATG 9 cut(s) 82, 151, 160, 439, 478, 504, 927, 943, 1003
BveI ACCTGC 1 cut(s) 1075
Cac8I GCNNGC 5 cut(s) 229, 363, 454, 458, 1119
CciI TCATGA 1 cut(s) 556
CfoI GCGC 6 cut(s) 15, 363, 365, 551, 688, 818
Cfr10I RCCGGY 2 cut(s) 227, 456
Cfr13I GGNCC 2 cut(s) 525, 951
CseI GACGC 3 cut(s) 71, 233, 257
Csp6I GTAC 3 cut(s) 95, 637, 958
CviAII CATG 7 cut(s) 46, 253, 310, 557, 689, 1022, 1130
CviQI GTAC 3 cut(s) 95, 637, 958
DdeI CTNAG 6 cut(s) 858, 870, 930, 968, 1090, 1140
DpnI GATC 2 cut(s) 378, 415
DpnII GATC 2 cut(s) 376, 413
DrdI GACNNNNNNGTC 1 cut(s) 532
DseDI GACNNNNNNGTC 1 cut(s) 532
EaeI YGGCCR 1 cut(s) 454
Eam1104I CTCTTC 3 cut(s) 78, 779, 857
EarI CTCTTC 3 cut(s) 78, 779, 857
EciI GGCGGA 2 cut(s) 383, 908
Ecl136II GAGCTC 1 cut(s) 1044
Eco130I CCWWGG 2 cut(s) 45, 792
Eco24I GRGCYC 1 cut(s) 1046
Eco31I GGTCTC 4 cut(s) 62, 230, 434, 1093
Eco47I GGWCC 2 cut(s) 525, 951
Eco47III AGCGCT 1 cut(s) 817
Eco53kI GAGCTC 1 cut(s) 1044
Eco57I CTGAAG 3 cut(s) 599, 647, 716
EcoICRI GAGCTC 1 cut(s) 1044
EcoRI GAATTC 1 cut(s) 560
EcoRII CCWGG 1 cut(s) 335
EcoT14I CCWWGG 2 cut(s) 45, 792
EcoT22I ATGCAT 1 cut(s) 1023
EcoT38I GRGCYC 1 cut(s) 1046
ErhI CCWWGG 2 cut(s) 45, 792
FaeI CATG 7 cut(s) 49, 256, 313, 560, 692, 1025, 1133
FaqI GGGAC 2 cut(s) 538, 836
FatI CATG 7 cut(s) 45, 252, 309, 556, 688, 1021, 1129
FauI CCCGC 3 cut(s) 40, 331, 911
Fnu4HI GCNGC 6 cut(s) 36, 348, 366, 424, 427, 654
FokI GGATG 9 cut(s) 89, 158, 167, 446, 485, 511, 934, 950, 1010
FriOI GRGCYC 1 cut(s) 1046
Fsp4HI GCNGC 6 cut(s) 36, 348, 366, 424, 427, 654
FspBI CTAG 1 cut(s) 537
GlaI GCGC 6 cut(s) 14, 362, 364, 550, 687, 817
GluI GCNGC 6 cut(s) 36, 348, 366, 424, 427, 654
HaeII RGCGCY 2 cut(s) 16, 819
HaeIII GGCC 3 cut(s) 456, 515, 1052
HapII CCGG 4 cut(s) 228, 353, 457, 824
HgaI GACGC 3 cut(s) 71, 233, 257
HhaI GCGC 6 cut(s) 15, 363, 365, 551, 688, 818
Hin1I GRCGYC 5 cut(s) 63, 105, 225, 249, 588
Hin1II CATG 7 cut(s) 49, 256, 313, 560, 692, 1025, 1133
Hin6I GCGC 6 cut(s) 13, 361, 363, 549, 686, 816
HinP1I GCGC 6 cut(s) 13, 361, 363, 549, 686, 816
HincII GTYRAC 2 cut(s) 592, 808
HindII GTYRAC 2 cut(s) 592, 808
HindIII AAGCTT 1 cut(s) 1115
HinfI GANTC 8 cut(s) 56, 212, 242, 317, 392, 932, 974, 1154
HpaII CCGG 4 cut(s) 228, 353, 457, 824
HphI GGTGA 3 cut(s) 146, 274, 761
Hpy166II GTNNAC 2 cut(s) 592, 808
Hpy188I TCNGA 9 cut(s) 61, 211, 223, 235, 247, 277, 387, 937, 1093
Hpy188III TCNNGA 4 cut(s) 557, 565, 967, 1167
Hpy8I GTNNAC 2 cut(s) 592, 808
Hpy99I CGWCG 6 cut(s) 65, 107, 110, 173, 227, 251
HpyAV CCTTC 6 cut(s) 403, 442, 488, 574, 883, 1041
HpyCH4III ACNGT 3 cut(s) 73, 286, 767
HpyCH4IV ACGT 2 cut(s) 105, 588
HpyCH4V TGCA 3 cut(s) 1021, 1084, 1121
HpyF10VI GCNNNNNNNGC 5 cut(s) 224, 353, 362, 546, 1027
HpyF3I CTNAG 6 cut(s) 858, 870, 930, 968, 1090, 1140
HpySE526I ACGT 2 cut(s) 105, 588
Hsp92I GRCGYC 5 cut(s) 63, 105, 225, 249, 588
Hsp92II CATG 7 cut(s) 49, 256, 313, 560, 692, 1025, 1133
HspAI GCGC 6 cut(s) 13, 361, 363, 549, 686, 816
KroI GCCGGC 2 cut(s) 227, 456
KroNI GCCGGC 2 cut(s) 229, 458
Kzo9I GATC 2 cut(s) 376, 413
LmnI GCTCC 5 cut(s) 10, 236, 355, 573, 1041
Lsp1109I GCAGC 4 cut(s) 22, 334, 435, 640
LweI GCATC 1 cut(s) 1008
MaeI CTAG 1 cut(s) 537
MaeII ACGT 2 cut(s) 105, 588
MaeIII GTNAC 1 cut(s) 184
MalI GATC 2 cut(s) 378, 415
MboI GATC 2 cut(s) 376, 413
MfeI CAATTG 1 cut(s) 1035
MflI RGATCY 1 cut(s) 413
MhlI GDGCHC 1 cut(s) 1046
MluCI AATT 2 cut(s) 560, 1035
MlyI GAGTC 5 cut(s) 50, 251, 311, 386, 941
MmeI TCCRAC 5 cut(s) 84, 246, 270, 441, 915
Mph1103I ATGCAT 1 cut(s) 1023
MroNI GCCGGC 2 cut(s) 227, 456
MroXI GAANNNNTTC 1 cut(s) 1030
MspA1I CMGCKG 3 cut(s) 35, 347, 426
MspI CCGG 4 cut(s) 228, 353, 457, 824
MspR9I CCNGG 1 cut(s) 337
MunI CAATTG 1 cut(s) 1035
Mva1269I GAATGC 1 cut(s) 1032
MvaI CCWGG 1 cut(s) 337
MvnI CGCG 3 cut(s) 363, 365, 551
MwoI GCNNNNNNNGC 5 cut(s) 224, 353, 362, 546, 1027
NaeI GCCGGC 2 cut(s) 229, 458
NcoI CCATGG 1 cut(s) 45
NdeII GATC 2 cut(s) 376, 413
NgoMIV GCCGGC 2 cut(s) 227, 456
NlaIII CATG 7 cut(s) 49, 256, 313, 560, 692, 1025, 1133
NlaIV GGNNCC 5 cut(s) 6, 232, 415, 526, 575
NmeAIII GCCGAG 2 cut(s) 91, 804
NmuCI GTSAC 1 cut(s) 184
NsiI ATGCAT 1 cut(s) 1023
NspV TTCGAA 1 cut(s) 680
PagI TCATGA 1 cut(s) 556
PauI GCGCGC 1 cut(s) 361
PcsI WCGNNNNNNNCGW 4 cut(s) 69, 114, 231, 426
PctI GAATGC 1 cut(s) 1032
PdiI GCCGGC 2 cut(s) 229, 458
PdmI GAANNNNTTC 1 cut(s) 1030
PfeI GAWTC 3 cut(s) 212, 974, 1154
PflFI GACNNNGTC 1 cut(s) 241
PflMI CCANNNNNTGG 1 cut(s) 607
PkrI GCNGC 6 cut(s) 37, 349, 367, 425, 428, 655
PleI GAGTC 5 cut(s) 50, 250, 311, 386, 940
PpsI GAGTC 5 cut(s) 50, 250, 311, 386, 940
Psp124BI GAGCTC 1 cut(s) 1046
Psp6I CCWGG 1 cut(s) 335
PspGI CCWGG 1 cut(s) 335
PspN4I GGNNCC 5 cut(s) 6, 232, 415, 526, 575
PspPI GGNCC 2 cut(s) 525, 951
PsuI RGATCY 1 cut(s) 413
PsyI GACNNNGTC 1 cut(s) 241
PteI GCGCGC 1 cut(s) 361
PvuII CAGCTG 1 cut(s) 347
RsaI GTAC 3 cut(s) 96, 638, 959
RsaNI GTAC 3 cut(s) 95, 637, 958
SacI GAGCTC 1 cut(s) 1046
SatI GCNGC 6 cut(s) 36, 348, 366, 424, 427, 654
Sau3AI GATC 2 cut(s) 376, 413
Sau96I GGNCC 2 cut(s) 525, 951
ScaI AGTACT 1 cut(s) 638
SchI GAGTC 5 cut(s) 50, 251, 311, 386, 941
ScrFI CCNGG 1 cut(s) 337
SduI GDGCHC 1 cut(s) 1046
SfaNI GCATC 1 cut(s) 1008
SfuI TTCGAA 1 cut(s) 680
SinI GGWCC 2 cut(s) 525, 951
SmlI CTYRAG 1 cut(s) 565
SmoI CTYRAG 1 cut(s) 565
Sse9I AATT 2 cut(s) 560, 1035
SsiI CCGC 9 cut(s) 33, 270, 324, 365, 368, 426, 893, 918, 982
SspMI CTAG 1 cut(s) 537
SstI GAGCTC 1 cut(s) 1046
StyD4I CCNGG 1 cut(s) 335
StyI CCWWGG 2 cut(s) 45, 792
TaaI ACNGT 3 cut(s) 73, 286, 767
TaiI ACGT 2 cut(s) 108, 591
TaqI TCGA 3 cut(s) 680, 1157, 1168
TaqII GACCGA 2 cut(s) 254, 542
TasI AATT 2 cut(s) 560, 1035
TatI WGTACW 1 cut(s) 636
TauI GCSGC 2 cut(s) 368, 429
TfiI GAWTC 3 cut(s) 212, 974, 1154
TseFI GTSAC 1 cut(s) 184
TseI GCWGC 4 cut(s) 35, 347, 423, 653
Tsp45I GTSAC 1 cut(s) 184
TspDTI ATGAA 8 cut(s) 93, 171, 326, 545, 573, 914, 1146, 1175
Tth111I GACNNNGTC 1 cut(s) 241
Van91I CCANNNNNTGG 1 cut(s) 607
VpaK11BI GGWCC 2 cut(s) 525, 951
XapI RAATTY 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 1030
XspI CTAG 1 cut(s) 537
ZraI GACGTC 2 cut(s) 106, 589
ZrmI AGTACT 1 cut(s) 638
Zsp2I ATGCAT 1 cut(s) 1023
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.