RLG00000021213

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0008960
Physical Location & Seq
Forward (+)
72968742 .. 72971028
2287 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000021213

Sequence Viewer

Length: 1263 bp
ATGGCTCCCAAGCGCCTCCTCCAAGACCCTCCCGCTGCTTCATCAATGGAGGAAGACTCCGACGCAGAGACCCTGGATGAAGAACAAGAAGAAGAAGAAGAAGACGAGGAACAAAACGACGCCGTTCAAGAAAAATCTGATGAGGAAGAAGAGGAGGAGGAAGAAGAAGAAGATAATGAGGAAGAGGAGAATGTGAATGCTAAGAAGCCCGTTGTGATTCCCTCATCGTCAGTTGTTCCAAACCCTCAGTCGGAATCGGCCTCCTCCTCCGATGACGACGCCGCCGGGTCGGAGACCGAGTCCGACGACATCAACCACTCTCCATCCGCTTCCGATTTCACCGTCAAGCCCATTGTCTCCAAGCCCGTGAACGACTCCTCCTCCGCCAAGCCCACCAAGAAGCCCTCTCCCGCTCCGGCGAAGCCCGCTCCCGGGTCGAAGCGCCCGGCCGAGAGCGATCCGACTCCGAAAGACTCGAAGAAGAAGAAGAAGGGCGCCAACGGCGGCGGCGACGATGAGGACACCAAAAAGGGTCGGCTTTGGGGCGAGGACGATGAGATTGCGATCCTGAAGGGGATGATAGACTTCAAGGCCAAGAAGGGGATCGACCCGTCTAGTAATATGGGCGCGTTCCATGAGTTCTTGAAGAGAAGCTTGAAGGCTGACGTCAGCAAGAACCAGTTGATGGATAAGACCAGGAGGTTGAAGAAGAAGTACCATACCAATGCTGAGAAGGGCGAGAATGGGGAGGGCCCGGTGTTTTTGAAAGCGCATGAGTCCAAGTCGTTTGATTTGTCGAAGAAGATTTGGGGTGCCGAGGCTAATGGGGGAGATGATAGTGCAAAGAACAGTAGGAAGAAGCCGAGGAAGAGTGCCAAGGCTAACAACAACAACTCTTCTACAGTCTTGGCATTGCCTGTTTCGGATGCTGCGGCTGAGAGGAGTGTGGAGAAGAAAGTAAAAGCAGAGGAGATGAATGTTGCTAATGGCGGAGTTAAGGCTGCTGAGCCCGATGACTTTTGGTCCAAGTACCCATCGCTGAGTGATTCTTTGCGGCTTGGAAATTGCTCATCGAGGTGGGAGGGTTTGGAGAGAGTTATGATTCAGAAAATGCCTTCAATTGGGAGCTCAAAGGCTAAAGAGTGGGATGATAGGTGGAGGAAGTTGCAGGTCTTAGAGATGGAGTTATCTGTCAAGAAGCTTGATTTGATGCATGAACAGGCTAAGCTTTTATTGGATGGGATAAAGTCGCGGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

421

Amino Acids

46.15

Weight (kDa)

4.99

Isoelectric Point (pI)

61.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 179 - 271 1.4e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 669
Acc36I ACCTGC 1 cut(s) 1159
AccB1I GGYRCC 2 cut(s) 494, 812
AccB7I CCANNNNNTGG 1 cut(s) 685
AccBSI CCGCTC 2 cut(s) 413, 428
AccII CGCG 2 cut(s) 629, 1252
AclWI GGATC 3 cut(s) 452, 559, 611
AcoI YGGCCR 1 cut(s) 447
AcuI CTGAAG 1 cut(s) 590
AcyI GRCGYC 4 cut(s) 120, 279, 495, 666
AfaI GTAC 2 cut(s) 716, 1031
AfiI CCNNNNNNNGG 6 cut(s) 250, 431, 432, 600, 685, 1121
AgsI TTSAA 7 cut(s) 128, 589, 646, 658, 706, 766, 1119
AhdI GACNNNNNGTC 1 cut(s) 1021
AjnI CCWGG 2 cut(s) 72, 695
AloI GAACNNNNNNTCC 2 cut(s) 362, 394
AluBI AGCT 4 cut(s) 654, 1128, 1201, 1228
AluI AGCT 4 cut(s) 654, 1128, 1201, 1228
Alw21I GWGCWC 1 cut(s) 1130
Alw26I GTCTC 3 cut(s) 62, 287, 361
AlwI GGATC 3 cut(s) 452, 559, 611
Ama87I CYCGRG 1 cut(s) 431
AoxI GGCC 4 cut(s) 258, 447, 591, 751
ApaI GGGCCC 1 cut(s) 755
ApeKI GCWGC 3 cut(s) 35, 929, 1001
ArsI GACNNNNNNTTYG 2 cut(s) 233, 265
AspLEI GCGC 5 cut(s) 15, 444, 497, 629, 772
AspS9I GGNCC 3 cut(s) 751, 752, 1023
AsuC2I CCSGG 5 cut(s) 286, 432, 433, 446, 755
AsuHPI GGTGA 1 cut(s) 331
AvaI CYCGRG 1 cut(s) 431
AvaII GGWCC 1 cut(s) 1023
BaeGI GKGCMC 1 cut(s) 755
BanI GGYRCC 2 cut(s) 494, 812
BanII GRGCYC 3 cut(s) 755, 1011, 1130
BarI GAAGNNNNNNTAC 2 cut(s) 698, 730
BbsI GAAGAC 2 cut(s) 60, 108
Bbv12I GWGCWC 1 cut(s) 1130
BbvI GCAGC 3 cut(s) 22, 916, 988
BccI CCATC 5 cut(s) 331, 679, 1042, 1174, 1232
BceAI ACGGC 2 cut(s) 107, 517
BcgI CGANNNNNNTGC 2 cut(s) 542, 576
BciT130I CCWGG 2 cut(s) 74, 697
BcnI CCSGG 5 cut(s) 286, 432, 433, 446, 755
BcoDI GTCTC 3 cut(s) 62, 287, 361
BfaI CTAG 1 cut(s) 615
BfmI CTRYAG 1 cut(s) 900
BfoI RGCGCY 3 cut(s) 16, 445, 498
BfuAI ACCTGC 1 cut(s) 1159
BisI GCNGC 8 cut(s) 36, 282, 505, 508, 930, 933, 1002, 1055
BlpI GCTNAGC 2 cut(s) 1005, 1224
BlsI GCNGC 8 cut(s) 37, 283, 506, 509, 931, 934, 1003, 1056
Bme1390I CCNGG 7 cut(s) 74, 286, 432, 433, 446, 697, 755
Bme18I GGWCC 1 cut(s) 1023
BmeRI GACNNNNNGTC 1 cut(s) 1021
BmeT110I CYCGRG 1 cut(s) 431
BmgT120I GGNCC 3 cut(s) 751, 752, 1023
BmiI GGNNCC 4 cut(s) 6, 496, 753, 814
BmrFI CCNGG 7 cut(s) 74, 286, 432, 433, 446, 697, 755
BmsI GCATC 2 cut(s) 916, 1200
BpiI GAAGAC 2 cut(s) 60, 108
BplI GAGNNNNNCTC 2 cut(s) 41, 73
Bpu1102I GCTNAGC 2 cut(s) 1005, 1224
BpuMI CCSGG 5 cut(s) 286, 432, 433, 446, 755
BsaBI GATNNNNATC 1 cut(s) 563
BsaHI GRCGYC 4 cut(s) 120, 279, 495, 666
BsaI GGTCTC 2 cut(s) 62, 287
BsaJI CCNNGG 5 cut(s) 72, 431, 816, 863, 876
BsaXI ACNNNNNCTCC 6 cut(s) 284, 314, 362, 365, 392, 395
Bsc4I CCNNNNNNNGG 6 cut(s) 250, 431, 432, 600, 685, 1121
Bse1I ACTGG 1 cut(s) 679
Bse3DI GCAATG 1 cut(s) 911
Bse8I GATNNNNATC 1 cut(s) 563
BseBI CCWGG 2 cut(s) 74, 697
BseDI CCNNGG 5 cut(s) 72, 431, 816, 863, 876
BseGI GGATG 6 cut(s) 82, 323, 582, 931, 1153, 1243
BseJI GATNNNNATC 1 cut(s) 563
BseLI CCNNNNNNNGG 6 cut(s) 250, 431, 432, 600, 685, 1121
BseMI GCAATG 1 cut(s) 911
BseMII CTCAG 5 cut(s) 260, 720, 927, 996, 1031
BseNI ACTGG 1 cut(s) 679
BseSI GKGCMC 1 cut(s) 755
BseX3I CGGCCG 1 cut(s) 447
BseXI GCAGC 3 cut(s) 22, 916, 988
Bsh1236I CGCG 2 cut(s) 629, 1252
Bsh1285I CGRYCG 1 cut(s) 450
BshFI GGCC 4 cut(s) 260, 449, 593, 753
BshNI GGYRCC 2 cut(s) 494, 812
BsiEI CGRYCG 1 cut(s) 450
BsiHKAI GWGCWC 1 cut(s) 1130
BsiHKCI CYCGRG 1 cut(s) 431
BsiSI CCGG 5 cut(s) 285, 416, 432, 446, 755
BslI CCNNNNNNNGG 6 cut(s) 250, 431, 432, 600, 685, 1121
BsmAI GTCTC 3 cut(s) 62, 287, 361
BsmI GAATGC 1 cut(s) 202
BsnI GGCC 4 cut(s) 260, 449, 593, 753
Bso31I GGTCTC 2 cut(s) 62, 287
BsoBI CYCGRG 1 cut(s) 431
Bsp120I GGGCCC 1 cut(s) 751
Bsp1286I GDGCHC 3 cut(s) 755, 1011, 1130
Bsp143I GATC 3 cut(s) 457, 564, 603
Bsp1720I GCTNAGC 2 cut(s) 1005, 1224
BspANI GGCC 4 cut(s) 260, 449, 593, 753
BspCNI CTCAG 5 cut(s) 259, 721, 928, 997, 1032
BspFNI CGCG 2 cut(s) 629, 1252
BspLI GGNNCC 4 cut(s) 6, 496, 753, 814
BspMI ACCTGC 1 cut(s) 1159
BspPI GGATC 3 cut(s) 452, 559, 611
BspT107I GGYRCC 2 cut(s) 494, 812
BspTNI GGTCTC 2 cut(s) 62, 287
BsrBI CCGCTC 2 cut(s) 413, 428
BsrDI GCAATG 1 cut(s) 911
BsrI ACTGG 1 cut(s) 679
BssECI CCNNGG 5 cut(s) 72, 431, 816, 863, 876
BssMI GATC 3 cut(s) 457, 564, 603
BssNI GRCGYC 4 cut(s) 120, 279, 495, 666
BssT1I CCWWGG 1 cut(s) 876
Bst2UI CCWGG 2 cut(s) 74, 697
Bst4CI ACNGT 3 cut(s) 343, 851, 904
Bst6I CTCTTC 5 cut(s) 144, 177, 641, 863, 901
BstACI GRCGYC 4 cut(s) 120, 279, 495, 666
BstC8I GCNNGC 1 cut(s) 426
BstDEI CTNAG 8 cut(s) 201, 246, 729, 936, 1005, 1040, 1174, 1224
BstF5I GGATG 6 cut(s) 82, 323, 582, 931, 1153, 1243
BstFNI CGCG 2 cut(s) 629, 1252
BstH2I RGCGCY 3 cut(s) 16, 445, 498
BstHHI GCGC 5 cut(s) 15, 444, 497, 629, 772
BstKTI GATC 3 cut(s) 460, 567, 606
BstMAI GTCTC 3 cut(s) 62, 287, 361
BstMBI GATC 3 cut(s) 457, 564, 603
BstMCI CGRYCG 1 cut(s) 450
BstMWI GCNNNNNNNGC 2 cut(s) 425, 501
BstNI CCWGG 2 cut(s) 74, 697
BstSCI CCNGG 7 cut(s) 72, 284, 430, 431, 444, 695, 753
BstSFI CTRYAG 1 cut(s) 900
BstSLI GKGCMC 1 cut(s) 755
BstUI CGCG 2 cut(s) 629, 1252
BstV1I GCAGC 3 cut(s) 22, 916, 988
BstV2I GAAGAC 2 cut(s) 60, 108
BstZI CGGCCG 1 cut(s) 447
BsuRI GGCC 4 cut(s) 260, 449, 593, 753
BtgZI GCGATG 1 cut(s) 1020
BtsCI GGATG 6 cut(s) 82, 323, 582, 931, 1153, 1243
BveI ACCTGC 1 cut(s) 1159
Cac8I GCNNGC 1 cut(s) 426
CfoI GCGC 5 cut(s) 15, 444, 497, 629, 772
Cfr13I GGNCC 3 cut(s) 751, 752, 1023
Cfr9I CCCGGG 1 cut(s) 431
CseI GACGC 3 cut(s) 71, 128, 287
Csp6I GTAC 2 cut(s) 715, 1030
CviAII CATG 3 cut(s) 635, 773, 1214
CviQI GTAC 2 cut(s) 715, 1030
DdeI CTNAG 8 cut(s) 201, 246, 729, 936, 1005, 1040, 1174, 1224
DinI GGCGCC 1 cut(s) 496
DpnI GATC 3 cut(s) 459, 566, 605
DpnII GATC 3 cut(s) 457, 564, 603
DriI GACNNNNNGTC 1 cut(s) 1021
EaeI YGGCCR 1 cut(s) 447
EagI CGGCCG 1 cut(s) 447
Eam1104I CTCTTC 5 cut(s) 144, 177, 641, 863, 901
Eam1105I GACNNNNNGTC 1 cut(s) 1021
EarI CTCTTC 5 cut(s) 144, 177, 641, 863, 901
EciI GGCGGA 2 cut(s) 373, 1005
Ecl136II GAGCTC 1 cut(s) 1128
EclXI CGGCCG 1 cut(s) 447
Eco130I CCWWGG 1 cut(s) 876
Eco24I GRGCYC 3 cut(s) 755, 1011, 1130
Eco31I GGTCTC 2 cut(s) 62, 287
Eco47I GGWCC 1 cut(s) 1023
Eco52I CGGCCG 1 cut(s) 447
Eco53kI GAGCTC 1 cut(s) 1128
Eco57I CTGAAG 1 cut(s) 590
Eco88I CYCGRG 1 cut(s) 431
EcoICRI GAGCTC 1 cut(s) 1128
EcoO109I RGGNCCY 1 cut(s) 751
EcoRII CCWGG 2 cut(s) 72, 695
EcoT14I CCWWGG 1 cut(s) 876
EcoT22I ATGCAT 1 cut(s) 1215
EcoT38I GRGCYC 3 cut(s) 755, 1011, 1130
EgeI GGCGCC 1 cut(s) 496
EheI GGCGCC 1 cut(s) 496
ErhI CCWWGG 1 cut(s) 876
FaeI CATG 3 cut(s) 638, 776, 1217
FaiI YATR 6 cut(s) 623, 636, 720, 774, 1100, 1215
FalI AAGNNNNNCTT 2 cut(s) 638, 670
FatI CATG 3 cut(s) 634, 772, 1213
FauI CCCGC 3 cut(s) 40, 418, 433
Fnu4HI GCNGC 8 cut(s) 36, 282, 505, 508, 930, 933, 1002, 1055
FokI GGATG 6 cut(s) 89, 310, 589, 938, 1160, 1250
FriOI GRGCYC 3 cut(s) 755, 1011, 1130
Fsp4HI GCNGC 8 cut(s) 36, 282, 505, 508, 930, 933, 1002, 1055
FspBI CTAG 1 cut(s) 615
GlaI GCGC 5 cut(s) 14, 443, 496, 628, 771
GluI GCNGC 8 cut(s) 36, 282, 505, 508, 930, 933, 1002, 1055
HaeII RGCGCY 3 cut(s) 16, 445, 498
HaeIII GGCC 4 cut(s) 260, 449, 593, 753
HapII CCGG 5 cut(s) 285, 416, 432, 446, 755
HgaI GACGC 3 cut(s) 71, 128, 287
HhaI GCGC 5 cut(s) 15, 444, 497, 629, 772
Hin1I GRCGYC 4 cut(s) 120, 279, 495, 666
Hin1II CATG 3 cut(s) 638, 776, 1217
Hin6I GCGC 5 cut(s) 13, 442, 495, 627, 770
HinP1I GCGC 5 cut(s) 13, 442, 495, 627, 770
HindIII AAGCTT 3 cut(s) 652, 1199, 1226
HpaII CCGG 5 cut(s) 285, 416, 432, 446, 755
HphI GGTGA 1 cut(s) 331
Hpy166II GTNNAC 1 cut(s) 370
Hpy188III TCNNGA 4 cut(s) 128, 568, 643, 1195
Hpy8I GTNNAC 1 cut(s) 370
Hpy99I CGWCG 5 cut(s) 65, 122, 281, 308, 515
HpyAV CCTTC 7 cut(s) 484, 565, 592, 652, 727, 1125, 1249
HpyCH4III ACNGT 3 cut(s) 343, 851, 904
HpyCH4IV ACGT 1 cut(s) 666
HpyCH4V TGCA 3 cut(s) 842, 1168, 1213
HpyF10VI GCNNNNNNNGC 2 cut(s) 425, 501
HpyF3I CTNAG 8 cut(s) 201, 246, 729, 936, 1005, 1040, 1174, 1224
HpySE526I ACGT 1 cut(s) 666
Hsp92I GRCGYC 4 cut(s) 120, 279, 495, 666
Hsp92II CATG 3 cut(s) 638, 776, 1217
HspAI GCGC 5 cut(s) 13, 442, 495, 627, 770
KasI GGCGCC 1 cut(s) 494
Kzo9I GATC 3 cut(s) 457, 564, 603
LmnI GCTCC 4 cut(s) 10, 418, 433, 1125
Lsp1109I GCAGC 3 cut(s) 22, 916, 988
LweI GCATC 2 cut(s) 916, 1200
MaeI CTAG 1 cut(s) 615
MaeII ACGT 1 cut(s) 666
MalI GATC 3 cut(s) 459, 566, 605
MbiI CCGCTC 2 cut(s) 413, 428
MboI GATC 3 cut(s) 457, 564, 603
MfeI CAATTG 1 cut(s) 1119
MhlI GDGCHC 3 cut(s) 755, 1011, 1130
MluCI AATT 2 cut(s) 1063, 1119
Mly113I GGCGCC 1 cut(s) 495
MlyI GAGTC 6 cut(s) 50, 308, 368, 457, 467, 785
MmeI TCCRAC 5 cut(s) 84, 231, 270, 327, 485
Mph1103I ATGCAT 1 cut(s) 1215
MseI TTAA 1 cut(s) 996
MslI CAYNNNNRTG 2 cut(s) 723, 1075
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 5 cut(s) 285, 416, 432, 446, 755
MspR9I CCNGG 7 cut(s) 74, 286, 432, 433, 446, 697, 755
MunI CAATTG 1 cut(s) 1119
Mva1269I GAATGC 1 cut(s) 202
MvaI CCWGG 2 cut(s) 74, 697
MvnI CGCG 2 cut(s) 629, 1252
MwoI GCNNNNNNNGC 2 cut(s) 425, 501
NarI GGCGCC 1 cut(s) 495
NciI CCSGG 5 cut(s) 286, 432, 433, 446, 755
NdeII GATC 3 cut(s) 457, 564, 603
NlaIII CATG 3 cut(s) 638, 776, 1217
NlaIV GGNNCC 4 cut(s) 6, 496, 753, 814
NmeAIII GCCGAG 3 cut(s) 475, 841, 888
NsiI ATGCAT 1 cut(s) 1215
PcsI WCGNNNNNNNCGW 1 cut(s) 507
PctI GAATGC 1 cut(s) 202
PfeI GAWTC 4 cut(s) 217, 254, 1046, 1102
PflFI GACNNNGTC 1 cut(s) 298
PflMI CCANNNNNTGG 1 cut(s) 685
PkrI GCNGC 8 cut(s) 37, 283, 506, 509, 931, 934, 1003, 1056
PleI GAGTC 6 cut(s) 50, 307, 368, 457, 467, 784
PluTI GGCGCC 1 cut(s) 498
PpsI GAGTC 6 cut(s) 50, 307, 368, 457, 467, 784
Psp124BI GAGCTC 1 cut(s) 1130
Psp6I CCWGG 2 cut(s) 72, 695
PspGI CCWGG 2 cut(s) 72, 695
PspN4I GGNNCC 4 cut(s) 6, 496, 753, 814
PspOMI GGGCCC 1 cut(s) 751
PspPI GGNCC 3 cut(s) 751, 752, 1023
PsyI GACNNNGTC 1 cut(s) 298
RsaI GTAC 2 cut(s) 716, 1031
RsaNI GTAC 2 cut(s) 715, 1030
RseI CAYNNNNRTG 2 cut(s) 723, 1075
SacI GAGCTC 1 cut(s) 1130
SaqAI TTAA 1 cut(s) 996
SatI GCNGC 8 cut(s) 36, 282, 505, 508, 930, 933, 1002, 1055
Sau3AI GATC 3 cut(s) 457, 564, 603
Sau96I GGNCC 3 cut(s) 751, 752, 1023
SchI GAGTC 6 cut(s) 50, 308, 368, 457, 467, 785
ScrFI CCNGG 7 cut(s) 74, 286, 432, 433, 446, 697, 755
SduI GDGCHC 3 cut(s) 755, 1011, 1130
SetI ASST 9 cut(s) 656, 669, 704, 1079, 1130, 1157, 1173, 1203, 1230
SfaNI GCATC 2 cut(s) 916, 1200
SfcI CTRYAG 1 cut(s) 900
SfoI GGCGCC 1 cut(s) 496
SinI GGWCC 1 cut(s) 1023
SmaI CCCGGG 1 cut(s) 433
SmiMI CAYNNNNRTG 2 cut(s) 723, 1075
Sse9I AATT 2 cut(s) 1063, 1119
SspDI GGCGCC 1 cut(s) 494
SspMI CTAG 1 cut(s) 615
SstI GAGCTC 1 cut(s) 1130
StyD4I CCNGG 7 cut(s) 72, 284, 430, 431, 444, 695, 753
StyI CCWWGG 1 cut(s) 876
TaaI ACNGT 3 cut(s) 343, 851, 904
TaiI ACGT 1 cut(s) 669
TaqI TCGA 5 cut(s) 437, 476, 606, 797, 1073
TaqII GACCGA 1 cut(s) 311
TasI AATT 2 cut(s) 1063, 1119
TauI GCSGC 5 cut(s) 284, 507, 510, 935, 1057
TfiI GAWTC 4 cut(s) 217, 254, 1046, 1102
Tru1I TTAA 1 cut(s) 996
Tru9I TTAA 1 cut(s) 996
TseI GCWGC 3 cut(s) 35, 929, 1001
TspDTI ATGAA 4 cut(s) 30, 93, 989, 1230
TspMI CCCGGG 1 cut(s) 431
Tth111I GACNNNGTC 1 cut(s) 298
Van91I CCANNNNNTGG 1 cut(s) 685
VpaK11BI GGWCC 1 cut(s) 1023
XmaI CCCGGG 1 cut(s) 431
XspI CTAG 1 cut(s) 615
ZraI GACGTC 1 cut(s) 667
Zsp2I ATGCAT 1 cut(s) 1215
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.