RLG00000033523

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0008960
Physical Location & Seq
Forward (+)
28455951 .. 28457523
1573 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000033523

Sequence Viewer

Length: 888 bp
ATGACAGAGGCTGCTTGTTGTACGAATATGATGACTAGCATAATCAGAGCACTAGTGAAAGCCATTCACTCTACTCCCACGCAGTTCGTTCTCTATCTCTCCGGCGGAGCTTCTCAGGCTGTGGCGTGGTCACTCTCCGTTCCTGGAGCCACAAGTACAGTCTTGGAAACTATATTGCCTTATTCCCAAATGTCTATGATCCAATTACTCGGCAAGATTCCGGACAAGTTTTGTAGCCAACACACTGGTTTACGAAATCGAGAGGGAGAAGATATGGTTGCAAGTCATCTTGTACTCAAGGTGGCTTGCATCCATATCTTAGAAATAAAGAAATACCATACCAATGCTGAGAAGGGCAAGAATGGGGAGGGCTCGGTGTTTTCGAAAGCTCATGAGTCGAAGTCGTTTGATTTGTCGAAGAAGATTTGGGGTGCTGAGGCTAATGGGGGAGATGATAGTGCAAAGAACAGTAGGAAGAAGCTGAGGAAGAGTGCCAAGGCTAACAACAACAACAACAACTCTTCTACAGTCTTGGCATTGCCTGTTTCGGATGTTGGGGCTGAGAGGAATGTGGAGAAGAAAGTAAAAGTGAAGGAGATGAATGTTGCTAATGGCAGAGTTCAGGCTGCTGAGCCCGATGACTTTTGGTCAAAGTACCCATCACTGAGTGATTCTTTGCAGCTTGGAAATTGCTCATCAAGGTGGGAGGGTTTGGAGAGAGTTATGATTCAGAAAATGCCTTCAATTGGGAGCTCAAAGGCTAAAGAGTGGGATGATAGGTGGAGAAAGTTACAGGTCTTAGAGATGGAGTTATCTGTCAAGAAGCTTGATTTGATGCATGAACAGGCTAAGCTTTTATTGGATGGGATAAAGTCACGGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

296

Amino Acids

32.58

Weight (kDa)

9.29

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 110 - 144 4.4e-06 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 220
AciI CCGC 1 cut(s) 105
AclWI GGATC 1 cut(s) 193
AdeI CACNNNGTG 1 cut(s) 668
AfaI GTAC 4 cut(s) 22, 157, 294, 656
AfiI CCNNNNNNNGG 1 cut(s) 746
AgsI TTSAA 1 cut(s) 744
AhdI GACNNNNNGTC 1 cut(s) 646
AhlI ACTAGT 1 cut(s) 52
AjnI CCWGG 1 cut(s) 142
AluBI AGCT 7 cut(s) 110, 389, 481, 682, 753, 826, 853
AluI AGCT 7 cut(s) 110, 389, 481, 682, 753, 826, 853
Alw21I GWGCWC 2 cut(s) 52, 755
AlwI GGATC 1 cut(s) 193
AlwNI CAGNNNCTG 1 cut(s) 11
Aor13HI TCCGGA 1 cut(s) 220
ApeKI GCWGC 3 cut(s) 11, 626, 679
AsuII TTCGAA 1 cut(s) 383
BanII GRGCYC 3 cut(s) 374, 636, 755
Bbv12I GWGCWC 2 cut(s) 52, 755
BbvCI CCTCAGC 2 cut(s) 435, 482
BbvI GCAGC 2 cut(s) 613, 691
BccI CCATC 3 cut(s) 667, 799, 857
BciT130I CCWGG 1 cut(s) 144
BcuI ACTAGT 1 cut(s) 52
BfaI CTAG 2 cut(s) 36, 53
BfmI CTRYAG 1 cut(s) 525
BisI GCNGC 3 cut(s) 12, 627, 680
BlpI GCTNAGC 2 cut(s) 630, 849
BlsI GCNGC 3 cut(s) 13, 628, 681
Bme1390I CCNGG 1 cut(s) 144
BmeRI GACNNNNNGTC 1 cut(s) 646
BmiI GGNNCC 1 cut(s) 148
BmrFI CCNGG 1 cut(s) 144
BmsI GCATC 2 cut(s) 318, 825
BpmI CTGGAG 1 cut(s) 165
Bpu10I CCTNAGC 2 cut(s) 435, 482
Bpu1102I GCTNAGC 2 cut(s) 630, 849
Bpu14I TTCGAA 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 281
BsaJI CCNNGG 1 cut(s) 495
BsaWI WCCGGW 1 cut(s) 220
Bsc4I CCNNNNNNNGG 1 cut(s) 746
Bse1I ACTGG 1 cut(s) 250
Bse3DI GCAATG 1 cut(s) 536
BseAI TCCGGA 1 cut(s) 220
BseBI CCWGG 1 cut(s) 144
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 4 cut(s) 309, 556, 778, 868
BseLI CCNNNNNNNGG 1 cut(s) 746
BseMI GCAATG 1 cut(s) 536
BseMII CTCAG 7 cut(s) 128, 339, 426, 473, 552, 621, 656
BseNI ACTGG 1 cut(s) 250
BseXI GCAGC 2 cut(s) 613, 691
BsiHKAI GWGCWC 2 cut(s) 52, 755
BsiSI CCGG 2 cut(s) 102, 221
BslI CCNNNNNNNGG 1 cut(s) 746
Bsp119I TTCGAA 1 cut(s) 383
Bsp1286I GDGCHC 4 cut(s) 52, 374, 636, 755
Bsp13I TCCGGA 1 cut(s) 220
Bsp143I GATC 1 cut(s) 198
Bsp1720I GCTNAGC 2 cut(s) 630, 849
BspACI CCGC 1 cut(s) 105
BspCNI CTCAG 7 cut(s) 127, 340, 427, 474, 553, 622, 657
BspEI TCCGGA 1 cut(s) 220
BspHI TCATGA 1 cut(s) 391
BspLI GGNNCC 1 cut(s) 148
BspPI GGATC 1 cut(s) 193
BspT104I TTCGAA 1 cut(s) 383
BsrDI GCAATG 1 cut(s) 536
BsrI ACTGG 1 cut(s) 250
BssECI CCNNGG 1 cut(s) 495
BssMI GATC 1 cut(s) 198
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 144
Bst4CI ACNGT 3 cut(s) 160, 470, 529
Bst6I CTCTTC 2 cut(s) 482, 526
BstBI TTCGAA 1 cut(s) 383
BstC8I GCNNGC 1 cut(s) 307
BstF5I GGATG 4 cut(s) 309, 556, 778, 868
BstKTI GATC 1 cut(s) 201
BstMBI GATC 1 cut(s) 198
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstNI CCWGG 1 cut(s) 144
BstSCI CCNGG 1 cut(s) 142
BstSFI CTRYAG 1 cut(s) 525
BstV1I GCAGC 2 cut(s) 613, 691
BstXI CCANNNNNNTGG 1 cut(s) 245
BtsCI GGATG 4 cut(s) 309, 556, 778, 868
BtsIMutI CAGTG 2 cut(s) 243, 662
Cac8I GCNNGC 1 cut(s) 307
CaiI CAGNNNCTG 1 cut(s) 11
CciI TCATGA 1 cut(s) 391
Csp6I GTAC 4 cut(s) 21, 156, 293, 655
CviAII CATG 2 cut(s) 392, 839
CviQI GTAC 4 cut(s) 21, 156, 293, 655
DpnI GATC 1 cut(s) 200
DpnII GATC 1 cut(s) 198
DraIII CACNNNGTG 1 cut(s) 668
DriI GACNNNNNGTC 1 cut(s) 646
Eam1104I CTCTTC 2 cut(s) 482, 526
Eam1105I GACNNNNNGTC 1 cut(s) 646
EarI CTCTTC 2 cut(s) 482, 526
EciI GGCGGA 1 cut(s) 120
Ecl136II GAGCTC 1 cut(s) 753
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 3 cut(s) 374, 636, 755
Eco53kI GAGCTC 1 cut(s) 753
EcoICRI GAGCTC 1 cut(s) 753
EcoRII CCWGG 1 cut(s) 142
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 840
EcoT38I GRGCYC 3 cut(s) 374, 636, 755
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 2 cut(s) 395, 842
FatI CATG 2 cut(s) 391, 838
Fnu4HI GCNGC 3 cut(s) 12, 627, 680
FokI GGATG 4 cut(s) 296, 563, 785, 875
FriOI GRGCYC 3 cut(s) 374, 636, 755
Fsp4HI GCNGC 3 cut(s) 12, 627, 680
FspBI CTAG 2 cut(s) 36, 53
GluI GCNGC 3 cut(s) 12, 627, 680
GsuI CTGGAG 1 cut(s) 165
HapII CCGG 2 cut(s) 102, 221
Hin1II CATG 2 cut(s) 395, 842
HindIII AAGCTT 2 cut(s) 824, 851
HinfI GANTC 4 cut(s) 217, 395, 671, 727
HpaII CCGG 2 cut(s) 102, 221
Hpy166II GTNNAC 1 cut(s) 251
Hpy188I TCNGA 3 cut(s) 47, 550, 732
Hpy188III TCNNGA 4 cut(s) 221, 260, 392, 820
Hpy8I GTNNAC 1 cut(s) 251
HpyAV CCTTC 4 cut(s) 346, 586, 750, 874
HpyCH4III ACNGT 3 cut(s) 160, 470, 529
HpyCH4V TGCA 5 cut(s) 281, 309, 461, 679, 838
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
Hsp92II CATG 2 cut(s) 395, 842
Kpn2I TCCGGA 1 cut(s) 220
Kzo9I GATC 1 cut(s) 198
LmnI GCTCC 3 cut(s) 107, 146, 750
Lsp1109I GCAGC 2 cut(s) 613, 691
LweI GCATC 2 cut(s) 318, 825
MaeI CTAG 2 cut(s) 36, 53
MaeIII GTNAC 3 cut(s) 129, 789, 873
MalI GATC 1 cut(s) 200
MboI GATC 1 cut(s) 198
MboII GAAGA 7 cut(s) 281, 430, 433, 487, 499, 513, 589
MfeI CAATTG 1 cut(s) 744
MhlI GDGCHC 4 cut(s) 52, 374, 636, 755
MluCI AATT 3 cut(s) 203, 688, 744
MlyI GAGTC 1 cut(s) 404
MnlI CCTC 6 cut(s) 256, 361, 430, 477, 558, 700
Mph1103I ATGCAT 1 cut(s) 840
MroI TCCGGA 1 cut(s) 220
MslI CAYNNNNRTG 2 cut(s) 342, 700
MspI CCGG 2 cut(s) 102, 221
MspR9I CCNGG 1 cut(s) 144
MunI CAATTG 1 cut(s) 744
MvaI CCWGG 1 cut(s) 144
MwoI GCNNNNNNNGC 1 cut(s) 116
NdeII GATC 1 cut(s) 198
NlaIII CATG 2 cut(s) 395, 842
NlaIV GGNNCC 1 cut(s) 148
NmeAIII GCCGAG 1 cut(s) 189
NmuCI GTSAC 2 cut(s) 129, 873
NsiI ATGCAT 1 cut(s) 840
NspV TTCGAA 1 cut(s) 383
PagI TCATGA 1 cut(s) 391
PcsI WCGNNNNNNNCGW 1 cut(s) 380
PfeI GAWTC 3 cut(s) 217, 671, 727
PfoI TCCNGGA 1 cut(s) 142
PkrI GCNGC 3 cut(s) 13, 628, 681
PleI GAGTC 1 cut(s) 403
PpsI GAGTC 1 cut(s) 403
Psp124BI GAGCTC 1 cut(s) 755
Psp6I CCWGG 1 cut(s) 142
PspGI CCWGG 1 cut(s) 142
PspN4I GGNNCC 1 cut(s) 148
PstNI CAGNNNCTG 1 cut(s) 11
RsaI GTAC 4 cut(s) 22, 157, 294, 656
RsaNI GTAC 4 cut(s) 21, 156, 293, 655
RseI CAYNNNNRTG 2 cut(s) 342, 700
SacI GAGCTC 1 cut(s) 755
SatI GCNGC 3 cut(s) 12, 627, 680
Sau3AI GATC 1 cut(s) 198
SchI GAGTC 1 cut(s) 404
ScrFI CCNGG 1 cut(s) 144
SduI GDGCHC 4 cut(s) 52, 374, 636, 755
SfaNI GCATC 2 cut(s) 318, 825
SfcI CTRYAG 1 cut(s) 525
SfuI TTCGAA 1 cut(s) 383
SmiMI CAYNNNNRTG 2 cut(s) 342, 700
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SpeI ACTAGT 1 cut(s) 52
Sse9I AATT 3 cut(s) 203, 688, 744
SsiI CCGC 1 cut(s) 105
SspMI CTAG 2 cut(s) 36, 53
SstI GAGCTC 1 cut(s) 755
StyD4I CCNGG 1 cut(s) 142
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 3 cut(s) 160, 470, 529
TaqI TCGA 4 cut(s) 259, 383, 398, 416
TasI AATT 3 cut(s) 203, 688, 744
TatI WGTACW 2 cut(s) 155, 292
TfiI GAWTC 3 cut(s) 217, 671, 727
TscAI CASTG 2 cut(s) 250, 669
TseFI GTSAC 2 cut(s) 129, 873
TseI GCWGC 3 cut(s) 11, 626, 679
Tsp45I GTSAC 2 cut(s) 129, 873
TspDTI ATGAA 2 cut(s) 614, 855
TspGWI ACGGA 1 cut(s) 127
TspRI CASTG 2 cut(s) 250, 669
XspI CTAG 2 cut(s) 36, 53
Zsp2I ATGCAT 1 cut(s) 840
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.