Rh2CG532700

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2C
OG0008960
Physical Location & Seq
Forward (+)
70817246 .. 70820152
2907 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2CG532700.1

Sequence Viewer

Length: 1281 bp
ATGGCTCCCAAGCGCCTCCTCCAAGACCCTCCCGCTGCTTCATCAATGGAGGAAGACTCCGACGCAGAGACCCTGGATGAAGATCAAGAACAAGAAGAAGAAGAAGAAGAGGAACAAAACGACGCCGTTCAAGAAAAATCTGATGAGGAAGAAGAGGAGGAGGAGGAGGAGGAAGAAGAAGAAGAAGAAGATAATGAGGAAGAGGAGAATGTGAGTGCTAAGAAGCCCGTTGTGATTCCCTCATCGTCAGTTGTTCCAAACCCTCAATCGGAATCGGCCTCCTCCGAAGACGACGACGCCGCCGGGTCGGAGACCGAGTCCGACGACATCAACCACTCGCCATCCGCTTCCGATTTCACCGTCAAGCCCATTGTCTCCAAGCCCGTGAACGACTCCTCCTCCGCCAAGCCCACCAAGAAGCCCGCTCCGGCTCCGGCGAAGCCCGCTCCCGGGTCGAAGCGCCCGGCCGAGAGCGATCCGACCCCGAAAGACTCGAAGAGGAAGAAGAAGGGCGCCAACGGCGGAGACGATGAGGACACCAAAAAGGGTCGGCTTTGGGGCGAGGACGATGAGATTGCGATCCTGAAGGGGATGATAGACTTCAAGGCCAGGAAGGGGATCGACCCGTCTTGTAACATGGGCGCGTTCCATGAGTTCTTGAAGAGAAGCTTGAAGGCTGACGTCAGCAAGAACCAGTTGATGGATAAGACCAGGAGGTTGAAGAAGAAGTACCATACGAATGCTGAGAAGGGCGAGAATGGGGAGGGCCCGGTGTTTTCGAAGGCGCATGAGTTGAAGTCGTTTGATTTGTCGAAGAAGATTTGGGGTGCTGAGGCTAACGGGGGAGATGATAGTGGAAAGAACAGTACGAAGAAGCCGAGGAAGAGTGCCAAGGCTAACAACAACAACAACTCTTCTACAGTCTTGGCATTGCCTGTTTCGGATGCTGCGGCTGAGAGGAGTGTGGAGAAGAAAGTAAAAGTGGAGGAGATGAATGTTGCTAATGGCGGAGTTAAGGCTGTTGAGTCCGATGACTTTTGGTCCAAGTACCCATCGCTGAGTGATTCTTTGCAGCTTGCAAATTGCTCATCGAGGTGGGAGGGTTTGGAGAGAGTTTTGATTCAGAAAATGCCTTCAATTGGGAGCTCAAAGGCTAAAGAGTTGGATGATAGGTGGAGGAAGTTGCAGGTCTTAGAGATGGAGTTATCTGTCAAGAAGCTTGATTTGATGCATGAACAGGCTAAGCTTTTATTGGATGGGATAAAGTCGCGGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

426

Amino Acids

46.93

Weight (kDa)

4.81

Isoelectric Point (pI)

65.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 184 - 276 5.8e-32 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 684
Acc36I ACCTGC 1 cut(s) 1177
AccB1I GGYRCC 1 cut(s) 512
AccB7I CCANNNNNTGG 1 cut(s) 700
AccBSI CCGCTC 2 cut(s) 425, 446
AccII CGCG 2 cut(s) 644, 1270
AclWI GGATC 3 cut(s) 470, 574, 626
AcoI YGGCCR 1 cut(s) 465
AcuI CTGAAG 1 cut(s) 605
AcyI GRCGYC 4 cut(s) 123, 297, 513, 681
AfaI GTAC 3 cut(s) 731, 868, 1049
AfiI CCNNNNNNNGG 6 cut(s) 268, 449, 450, 615, 700, 1139
AgsI TTSAA 7 cut(s) 131, 604, 661, 673, 721, 796, 1137
AhdI GACNNNNNGTC 1 cut(s) 1039
AjnI CCWGG 3 cut(s) 72, 608, 710
AloI GAACNNNNNNTCC 2 cut(s) 380, 412
AluBI AGCT 5 cut(s) 669, 1075, 1146, 1219, 1246
AluI AGCT 5 cut(s) 669, 1075, 1146, 1219, 1246
Alw21I GWGCWC 1 cut(s) 1148
Alw26I GTCTC 4 cut(s) 62, 305, 379, 519
AlwI GGATC 3 cut(s) 470, 574, 626
Ama87I CYCGRG 1 cut(s) 449
AoxI GGCC 4 cut(s) 276, 465, 606, 766
ApaI GGGCCC 1 cut(s) 770
ApeKI GCWGC 3 cut(s) 35, 947, 1072
AspLEI GCGC 5 cut(s) 15, 462, 515, 644, 787
AspS9I GGNCC 3 cut(s) 766, 767, 1041
AsuC2I CCSGG 5 cut(s) 304, 450, 451, 464, 770
AsuHPI GGTGA 1 cut(s) 349
AsuII TTCGAA 1 cut(s) 779
AvaI CYCGRG 1 cut(s) 449
AvaII GGWCC 1 cut(s) 1041
BaeGI GKGCMC 1 cut(s) 770
BanI GGYRCC 1 cut(s) 512
BanII GRGCYC 2 cut(s) 770, 1148
BarI GAAGNNNNNNTAC 2 cut(s) 713, 745
BbsI GAAGAC 2 cut(s) 60, 294
Bbv12I GWGCWC 1 cut(s) 1148
BbvCI CCTCAGC 1 cut(s) 831
BbvI GCAGC 3 cut(s) 22, 934, 1084
BccI CCATC 5 cut(s) 349, 694, 1060, 1192, 1250
BceAI ACGGC 2 cut(s) 110, 535
BcgI CGANNNNNNTGC 2 cut(s) 557, 591
BciT130I CCWGG 3 cut(s) 74, 610, 712
BcnI CCSGG 5 cut(s) 304, 450, 451, 464, 770
BcoDI GTCTC 4 cut(s) 62, 305, 379, 519
BfmI CTRYAG 1 cut(s) 918
BfoI RGCGCY 3 cut(s) 16, 463, 516
BfuAI ACCTGC 1 cut(s) 1177
BisI GCNGC 5 cut(s) 36, 300, 948, 951, 1073
BlpI GCTNAGC 1 cut(s) 1242
BlsI GCNGC 5 cut(s) 37, 301, 949, 952, 1074
Bme1390I CCNGG 8 cut(s) 74, 304, 450, 451, 464, 610, 712, 770
Bme18I GGWCC 1 cut(s) 1041
BmeRI GACNNNNNGTC 1 cut(s) 1039
BmeT110I CYCGRG 1 cut(s) 449
BmgT120I GGNCC 3 cut(s) 766, 767, 1041
BmiI GGNNCC 4 cut(s) 6, 432, 514, 768
BmrFI CCNGG 8 cut(s) 74, 304, 450, 451, 464, 610, 712, 770
BmsI GCATC 2 cut(s) 934, 1218
BpiI GAAGAC 2 cut(s) 60, 294
BplI GAGNNNNNCTC 2 cut(s) 41, 73
Bpu10I CCTNAGC 1 cut(s) 831
Bpu1102I GCTNAGC 1 cut(s) 1242
Bpu14I TTCGAA 1 cut(s) 779
BpuMI CCSGG 5 cut(s) 304, 450, 451, 464, 770
BsaBI GATNNNNATC 2 cut(s) 81, 578
BsaHI GRCGYC 4 cut(s) 123, 297, 513, 681
BsaI GGTCTC 2 cut(s) 62, 305
BsaJI CCNNGG 4 cut(s) 72, 449, 878, 891
BsaXI ACNNNNNCTCC 6 cut(s) 302, 332, 380, 383, 410, 413
Bsc4I CCNNNNNNNGG 6 cut(s) 268, 449, 450, 615, 700, 1139
Bse1I ACTGG 1 cut(s) 694
Bse3DI GCAATG 1 cut(s) 929
Bse8I GATNNNNATC 2 cut(s) 81, 578
BseBI CCWGG 3 cut(s) 74, 610, 712
BseDI CCNNGG 4 cut(s) 72, 449, 878, 891
BseGI GGATG 6 cut(s) 82, 341, 597, 949, 1171, 1261
BseJI GATNNNNATC 2 cut(s) 81, 578
BseLI CCNNNNNNNGG 6 cut(s) 268, 449, 450, 615, 700, 1139
BseMI GCAATG 1 cut(s) 929
BseMII CTCAG 4 cut(s) 735, 822, 945, 1049
BseNI ACTGG 1 cut(s) 694
BseSI GKGCMC 1 cut(s) 770
BseX3I CGGCCG 1 cut(s) 465
BseXI GCAGC 3 cut(s) 22, 934, 1084
Bsh1236I CGCG 2 cut(s) 644, 1270
Bsh1285I CGRYCG 1 cut(s) 468
BshFI GGCC 4 cut(s) 278, 467, 608, 768
BshNI GGYRCC 1 cut(s) 512
BsiEI CGRYCG 1 cut(s) 468
BsiHKAI GWGCWC 1 cut(s) 1148
BsiHKCI CYCGRG 1 cut(s) 449
BsiSI CCGG 6 cut(s) 303, 428, 434, 450, 464, 770
BslI CCNNNNNNNGG 6 cut(s) 268, 449, 450, 615, 700, 1139
BsmAI GTCTC 4 cut(s) 62, 305, 379, 519
BsmBI CGTCTC 1 cut(s) 519
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 4 cut(s) 278, 467, 608, 768
Bso31I GGTCTC 2 cut(s) 62, 305
BsoBI CYCGRG 1 cut(s) 449
Bsp119I TTCGAA 1 cut(s) 779
Bsp120I GGGCCC 1 cut(s) 766
Bsp1286I GDGCHC 2 cut(s) 770, 1148
Bsp143I GATC 4 cut(s) 82, 475, 579, 618
Bsp1720I GCTNAGC 1 cut(s) 1242
BspANI GGCC 4 cut(s) 278, 467, 608, 768
BspCNI CTCAG 4 cut(s) 736, 823, 946, 1050
BspFNI CGCG 2 cut(s) 644, 1270
BspLI GGNNCC 4 cut(s) 6, 432, 514, 768
BspMI ACCTGC 1 cut(s) 1177
BspPI GGATC 3 cut(s) 470, 574, 626
BspT104I TTCGAA 1 cut(s) 779
BspT107I GGYRCC 1 cut(s) 512
BspTNI GGTCTC 2 cut(s) 62, 305
BsrBI CCGCTC 2 cut(s) 425, 446
BsrDI GCAATG 1 cut(s) 929
BsrI ACTGG 1 cut(s) 694
BssECI CCNNGG 4 cut(s) 72, 449, 878, 891
BssMI GATC 4 cut(s) 82, 475, 579, 618
BssNI GRCGYC 4 cut(s) 123, 297, 513, 681
BssT1I CCWWGG 1 cut(s) 891
Bst2UI CCWGG 3 cut(s) 74, 610, 712
Bst4CI ACNGT 3 cut(s) 361, 866, 922
Bst6I CTCTTC 7 cut(s) 102, 147, 195, 491, 656, 878, 919
BstACI GRCGYC 4 cut(s) 123, 297, 513, 681
BstBI TTCGAA 1 cut(s) 779
BstC8I GCNNGC 3 cut(s) 423, 444, 1077
BstDEI CTNAG 7 cut(s) 219, 744, 831, 954, 1058, 1192, 1242
BstF5I GGATG 6 cut(s) 82, 341, 597, 949, 1171, 1261
BstFNI CGCG 2 cut(s) 644, 1270
BstH2I RGCGCY 3 cut(s) 16, 463, 516
BstHHI GCGC 5 cut(s) 15, 462, 515, 644, 787
BstKTI GATC 4 cut(s) 85, 478, 582, 621
BstMAI GTCTC 4 cut(s) 62, 305, 379, 519
BstMBI GATC 4 cut(s) 82, 475, 579, 618
BstMCI CGRYCG 1 cut(s) 468
BstMWI GCNNNNNNNGC 2 cut(s) 443, 519
BstNI CCWGG 3 cut(s) 74, 610, 712
BstSCI CCNGG 8 cut(s) 72, 302, 448, 449, 462, 608, 710, 768
BstSFI CTRYAG 1 cut(s) 918
BstSLI GKGCMC 1 cut(s) 770
BstUI CGCG 2 cut(s) 644, 1270
BstV1I GCAGC 3 cut(s) 22, 934, 1084
BstV2I GAAGAC 2 cut(s) 60, 294
BstZI CGGCCG 1 cut(s) 465
BsuRI GGCC 4 cut(s) 278, 467, 608, 768
BtgZI GCGATG 1 cut(s) 1038
BtsCI GGATG 6 cut(s) 82, 341, 597, 949, 1171, 1261
BveI ACCTGC 1 cut(s) 1177
Cac8I GCNNGC 3 cut(s) 423, 444, 1077
CfoI GCGC 5 cut(s) 15, 462, 515, 644, 787
Cfr13I GGNCC 3 cut(s) 766, 767, 1041
Cfr9I CCCGGG 1 cut(s) 449
CseI GACGC 3 cut(s) 71, 131, 305
Csp6I GTAC 3 cut(s) 730, 867, 1048
CviAII CATG 4 cut(s) 637, 650, 788, 1232
CviQI GTAC 3 cut(s) 730, 867, 1048
DdeI CTNAG 7 cut(s) 219, 744, 831, 954, 1058, 1192, 1242
DinI GGCGCC 1 cut(s) 514
DpnI GATC 4 cut(s) 84, 477, 581, 620
DpnII GATC 4 cut(s) 82, 475, 579, 618
DriI GACNNNNNGTC 1 cut(s) 1039
EaeI YGGCCR 1 cut(s) 465
EagI CGGCCG 1 cut(s) 465
Eam1104I CTCTTC 7 cut(s) 102, 147, 195, 491, 656, 878, 919
Eam1105I GACNNNNNGTC 1 cut(s) 1039
EarI CTCTTC 7 cut(s) 102, 147, 195, 491, 656, 878, 919
EciI GGCGGA 3 cut(s) 391, 537, 1023
Ecl136II GAGCTC 1 cut(s) 1146
EclXI CGGCCG 1 cut(s) 465
Eco130I CCWWGG 1 cut(s) 891
Eco24I GRGCYC 2 cut(s) 770, 1148
Eco31I GGTCTC 2 cut(s) 62, 305
Eco47I GGWCC 1 cut(s) 1041
Eco52I CGGCCG 1 cut(s) 465
Eco53kI GAGCTC 1 cut(s) 1146
Eco57I CTGAAG 1 cut(s) 605
Eco88I CYCGRG 1 cut(s) 449
EcoICRI GAGCTC 1 cut(s) 1146
EcoO109I RGGNCCY 1 cut(s) 766
EcoRII CCWGG 3 cut(s) 72, 608, 710
EcoT14I CCWWGG 1 cut(s) 891
EcoT22I ATGCAT 1 cut(s) 1233
EcoT38I GRGCYC 2 cut(s) 770, 1148
EgeI GGCGCC 1 cut(s) 514
EheI GGCGCC 1 cut(s) 514
ErhI CCWWGG 1 cut(s) 891
Esp3I CGTCTC 1 cut(s) 519
FaeI CATG 4 cut(s) 640, 653, 791, 1235
FaiI YATR 5 cut(s) 638, 651, 735, 789, 1233
FalI AAGNNNNNCTT 2 cut(s) 653, 685
FatI CATG 4 cut(s) 636, 649, 787, 1231
FauI CCCGC 3 cut(s) 40, 430, 451
Fnu4HI GCNGC 5 cut(s) 36, 300, 948, 951, 1073
FokI GGATG 6 cut(s) 89, 328, 604, 956, 1178, 1268
FriOI GRGCYC 2 cut(s) 770, 1148
Fsp4HI GCNGC 5 cut(s) 36, 300, 948, 951, 1073
GlaI GCGC 5 cut(s) 14, 461, 514, 643, 786
GluI GCNGC 5 cut(s) 36, 300, 948, 951, 1073
HaeII RGCGCY 3 cut(s) 16, 463, 516
HaeIII GGCC 4 cut(s) 278, 467, 608, 768
HapII CCGG 6 cut(s) 303, 428, 434, 450, 464, 770
HgaI GACGC 3 cut(s) 71, 131, 305
HhaI GCGC 5 cut(s) 15, 462, 515, 644, 787
Hin1I GRCGYC 4 cut(s) 123, 297, 513, 681
Hin1II CATG 4 cut(s) 640, 653, 791, 1235
Hin6I GCGC 5 cut(s) 13, 460, 513, 642, 785
HinP1I GCGC 5 cut(s) 13, 460, 513, 642, 785
HindIII AAGCTT 3 cut(s) 667, 1217, 1244
HinfI GANTC 9 cut(s) 56, 235, 272, 317, 392, 491, 1025, 1064, 1120
HpaII CCGG 6 cut(s) 303, 428, 434, 450, 464, 770
HphI GGTGA 1 cut(s) 349
Hpy166II GTNNAC 1 cut(s) 388
Hpy188III TCNNGA 5 cut(s) 86, 131, 583, 658, 1213
Hpy8I GTNNAC 1 cut(s) 388
Hpy99I CGWCG 5 cut(s) 65, 125, 296, 299, 326
HpyAV CCTTC 8 cut(s) 502, 580, 607, 667, 742, 775, 1143, 1267
HpyCH4III ACNGT 3 cut(s) 361, 866, 922
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 4 cut(s) 1072, 1079, 1186, 1231
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 519
HpyF3I CTNAG 7 cut(s) 219, 744, 831, 954, 1058, 1192, 1242
HpySE526I ACGT 1 cut(s) 681
Hsp92I GRCGYC 4 cut(s) 123, 297, 513, 681
Hsp92II CATG 4 cut(s) 640, 653, 791, 1235
HspAI GCGC 5 cut(s) 13, 460, 513, 642, 785
KasI GGCGCC 1 cut(s) 512
Kzo9I GATC 4 cut(s) 82, 475, 579, 618
LmnI GCTCC 5 cut(s) 10, 430, 436, 451, 1143
Lsp1109I GCAGC 3 cut(s) 22, 934, 1084
LweI GCATC 2 cut(s) 934, 1218
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 1 cut(s) 632
MalI GATC 4 cut(s) 84, 477, 581, 620
MbiI CCGCTC 2 cut(s) 425, 446
MboI GATC 4 cut(s) 82, 475, 579, 618
MfeI CAATTG 1 cut(s) 1137
MhlI GDGCHC 2 cut(s) 770, 1148
MluCI AATT 2 cut(s) 1081, 1137
Mly113I GGCGCC 1 cut(s) 513
MlyI GAGTC 5 cut(s) 50, 326, 386, 485, 1034
MmeI TCCRAC 5 cut(s) 84, 288, 345, 503, 1143
Mph1103I ATGCAT 1 cut(s) 1233
MseI TTAA 1 cut(s) 1014
MslI CAYNNNNRTG 2 cut(s) 738, 1093
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 6 cut(s) 303, 428, 434, 450, 464, 770
MspR9I CCNGG 8 cut(s) 74, 304, 450, 451, 464, 610, 712, 770
MunI CAATTG 1 cut(s) 1137
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 3 cut(s) 74, 610, 712
MvnI CGCG 2 cut(s) 644, 1270
MwoI GCNNNNNNNGC 2 cut(s) 443, 519
NarI GGCGCC 1 cut(s) 513
NciI CCSGG 5 cut(s) 304, 450, 451, 464, 770
NdeII GATC 4 cut(s) 82, 475, 579, 618
NlaIII CATG 4 cut(s) 640, 653, 791, 1235
NlaIV GGNNCC 4 cut(s) 6, 432, 514, 768
NmeAIII GCCGAG 2 cut(s) 493, 903
NsiI ATGCAT 1 cut(s) 1233
NspV TTCGAA 1 cut(s) 779
PcsI WCGNNNNNNNCGW 2 cut(s) 525, 875
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 4 cut(s) 235, 272, 1064, 1120
PflFI GACNNNGTC 1 cut(s) 316
PflMI CCANNNNNTGG 1 cut(s) 700
PkrI GCNGC 5 cut(s) 37, 301, 949, 952, 1074
PleI GAGTC 5 cut(s) 50, 325, 386, 485, 1033
PluTI GGCGCC 1 cut(s) 516
PpsI GAGTC 5 cut(s) 50, 325, 386, 485, 1033
Psp124BI GAGCTC 1 cut(s) 1148
Psp6I CCWGG 3 cut(s) 72, 608, 710
PspGI CCWGG 3 cut(s) 72, 608, 710
PspN4I GGNNCC 4 cut(s) 6, 432, 514, 768
PspOMI GGGCCC 1 cut(s) 766
PspPI GGNCC 3 cut(s) 766, 767, 1041
PsyI GACNNNGTC 1 cut(s) 316
RsaI GTAC 3 cut(s) 731, 868, 1049
RsaNI GTAC 3 cut(s) 730, 867, 1048
RseI CAYNNNNRTG 2 cut(s) 738, 1093
SacI GAGCTC 1 cut(s) 1148
SaqAI TTAA 1 cut(s) 1014
SatI GCNGC 5 cut(s) 36, 300, 948, 951, 1073
Sau3AI GATC 4 cut(s) 82, 475, 579, 618
Sau96I GGNCC 3 cut(s) 766, 767, 1041
SchI GAGTC 5 cut(s) 50, 326, 386, 485, 1034
ScrFI CCNGG 8 cut(s) 74, 304, 450, 451, 464, 610, 712, 770
SduI GDGCHC 2 cut(s) 770, 1148
SfaNI GCATC 2 cut(s) 934, 1218
SfcI CTRYAG 1 cut(s) 918
SfoI GGCGCC 1 cut(s) 514
SfuI TTCGAA 1 cut(s) 779
SinI GGWCC 1 cut(s) 1041
SmaI CCCGGG 1 cut(s) 451
SmiMI CAYNNNNRTG 2 cut(s) 738, 1093
Sse9I AATT 2 cut(s) 1081, 1137
SspDI GGCGCC 1 cut(s) 512
SstI GAGCTC 1 cut(s) 1148
StyD4I CCNGG 8 cut(s) 72, 302, 448, 449, 462, 608, 710, 768
StyI CCWWGG 1 cut(s) 891
TaaI ACNGT 3 cut(s) 361, 866, 922
TaiI ACGT 1 cut(s) 684
TaqI TCGA 6 cut(s) 455, 494, 621, 779, 812, 1091
TaqII GACCGA 1 cut(s) 329
TasI AATT 2 cut(s) 1081, 1137
TauI GCSGC 2 cut(s) 302, 953
TfiI GAWTC 4 cut(s) 235, 272, 1064, 1120
Tru1I TTAA 1 cut(s) 1014
Tru9I TTAA 1 cut(s) 1014
TseI GCWGC 3 cut(s) 35, 947, 1072
TspDTI ATGAA 4 cut(s) 30, 93, 1007, 1248
TspMI CCCGGG 1 cut(s) 449
Tth111I GACNNNGTC 1 cut(s) 316
Van91I CCANNNNNTGG 1 cut(s) 700
VpaK11BI GGWCC 1 cut(s) 1041
XmaI CCCGGG 1 cut(s) 449
ZraI GACGTC 1 cut(s) 682
Zsp2I ATGCAT 1 cut(s) 1233
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.