pycom09g02690

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr9
OG0008960
Physical Location & Seq
Reverse (-)
1995601 .. 1996803
1203 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom09g02690.1

Sequence Viewer

Length: 1203 bp
ATGGCTCCGAAGAAACTTGCAGAAGACCCGCCGGCCGCCGTGGTGGAGGACTCCGACGATGAAACCGTCGACGAAGAGGAAGAGGAAGAAGACGACCCCGTTGAAGTCAGCAAAGCAAAATCTGTGGATCTCAATGACGATGATTTAGAAGAGGAAGAGGAGGAAGAGGAAGAGGAGGAGGAGGAGGAGGAGGAAGAAAAGCCCTCTGCGGCGGTCACGAAGTCAAATCCCCAATCGGGTTTTGCCTCCGAAGACGATTCCGGATCGATCGGGTCGGAGACCGAGTCTGATAACCACTCGCCGTCAGCTTCCGACTTCACCGTCAAGCCGATCGTCTCCAAGCCCGCGAACAACGCCGCTATGCCCAGCAAGAAGACTGCTTCGGCGAAGCCGGCGGCCGCGCCCAGTTCCGGGTCGAAGCGACCGGCGGAGACCGAGGCCAACCCGAAAAACTCCAAGAAGAAGAAGGTCGGCGGCGACGATGAGGATGCTAAAAAGGGGCAGCGTTTTTGGAGCGAGGAGGATGAGATAACGATCCTGAAGGGCATTATAGAGTTCGAAAGCAAGAAAGGTGTGGCGGCGAGTTCGAATGTGGCCCCATTCCTTGAGTTCATCAAGAATGATTTGCAGGTCGATTTGAGCATCGACCAATTGGTTGAGAAGTTGACTGATAAGATCAGGAGATTGAAGAAGAAGTACCAGGCTGGAGAGGATGGCGAGGACCCGGTGTTTTCGAAGCCCCATGAGCTTAAGTGCTTTGAGCTGTGCAAAAAGATTTGGGGTTCTGAGGCCAATGGACTCGATAGCAATGTGAAGGGCGGTAAGAGGAAGCCGCCGAGGAAGAATAATAAGGTTAACACTGATACGGTTCTGGCCTTGCCGGTTTCAGATGCGTCTGTGAAGAAAGAGGTGCAGGATAATGTGACTAATAGAGGAGTTAAGGCTGACCCGGATGATTTTTGGTCTAAGTATCCATGTCTGAGTGGTTCTTTGAGGACGGGCAATTGTGCACCGAGGTCGGATTTAACTCGACTTTTGATTGAGAAGATGCCTTCTTTGGTTGGGAGTTCCAAGGCTAAAGAGTTGGAGGAGAGGTGGAGGGCATTGCAGCTCATTGAAATGGATTTGTCTGTCAAGAAGCTTGATTTGATGCAGGAACAGACTAAACTTGTATTGGATTCAATGAAGTCTTTGAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

401

Amino Acids

44.01

Weight (kDa)

4.71

Isoelectric Point (pI)

58.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 168 - 261 2.4e-27 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
Acc36I ACCTGC 1 cut(s) 619
AccI GTMKAC 1 cut(s) 69
AccII CGCG 2 cut(s) 347, 401
AccIII TCCGGA 1 cut(s) 260
AclWI GGATC 3 cut(s) 135, 271, 529
AcoI YGGCCR 2 cut(s) 33, 396
AcuI CTGAAG 1 cut(s) 560
AfaI GTAC 1 cut(s) 698
AfiI CCNNNNNNNGG 3 cut(s) 236, 410, 411
AflII CTTAAG 1 cut(s) 749
AgsI TTSAA 5 cut(s) 104, 688, 1118, 1182, 1195
AjnI CCWGG 1 cut(s) 699
AluBI AGCT 5 cut(s) 308, 748, 763, 1111, 1141
AluI AGCT 5 cut(s) 308, 748, 763, 1111, 1141
Alw21I GWGCWC 1 cut(s) 1012
Alw26I GTCTC 3 cut(s) 272, 340, 425
Alw44I GTGCAC 1 cut(s) 1008
AlwI GGATC 3 cut(s) 135, 271, 529
Aor13HI TCCGGA 1 cut(s) 260
AoxI GGCC 6 cut(s) 33, 396, 438, 594, 789, 873
ApaLI GTGCAC 1 cut(s) 1008
ApeKI GCWGC 2 cut(s) 502, 1108
AspLEI GCGC 1 cut(s) 403
AspS9I GGNCC 2 cut(s) 595, 721
AsuC2I CCSGG 3 cut(s) 412, 725, 950
AsuHPI GGTGA 1 cut(s) 310
AsuII TTCGAA 3 cut(s) 558, 587, 734
AvaII GGWCC 1 cut(s) 721
BaeGI GKGCMC 1 cut(s) 1012
BarI GAAGNNNNNNTAC 2 cut(s) 680, 712
BbsI GAAGAC 4 cut(s) 30, 96, 258, 380
Bbv12I GWGCWC 1 cut(s) 1012
BbvI GCAGC 2 cut(s) 514, 1120
BccI CCATC 1 cut(s) 707
BceAI ACGGC 2 cut(s) 23, 286
BcgI CGANNNNNNTGC 4 cut(s) 470, 504, 999, 1033
BciT130I CCWGG 1 cut(s) 701
BciVI GTATCC 1 cut(s) 981
BcnI CCSGG 3 cut(s) 412, 725, 950
BcoDI GTCTC 3 cut(s) 272, 340, 425
BfrI CTTAAG 1 cut(s) 749
BfuAI ACCTGC 1 cut(s) 619
BfuI GTATCC 1 cut(s) 981
Bme1390I CCNGG 4 cut(s) 412, 701, 725, 950
Bme18I GGWCC 1 cut(s) 721
BmgT120I GGNCC 2 cut(s) 595, 721
BmiI GGNNCC 3 cut(s) 6, 597, 723
BmrFI CCNGG 4 cut(s) 412, 701, 725, 950
BmrI ACTGGG 1 cut(s) 399
BmsI GCATC 5 cut(s) 478, 651, 880, 1038, 1140
BmuI ACTGGG 1 cut(s) 399
BpiI GAAGAC 4 cut(s) 30, 96, 258, 380
BpmI CTGGAG 1 cut(s) 726
Bpu14I TTCGAA 3 cut(s) 558, 587, 734
BpuEI CTTGAG 1 cut(s) 626
BpuMI CCSGG 3 cut(s) 412, 725, 950
Bsa29I ATCGAT 1 cut(s) 266
BsaBI GATNNNNATC 1 cut(s) 533
BsaI GGTCTC 2 cut(s) 272, 425
BsaJI CCNNGG 5 cut(s) 39, 435, 836, 1013, 1071
BsaWI WCCGGW 1 cut(s) 260
BsaXI ACNNNNNCTCC 4 cut(s) 269, 299, 1079, 1109
Bsc4I CCNNNNNNNGG 3 cut(s) 236, 410, 411
Bse118I RCCGGY 4 cut(s) 31, 391, 424, 880
Bse1I ACTGG 1 cut(s) 405
Bse3DI GCAATG 2 cut(s) 814, 1103
Bse8I GATNNNNATC 1 cut(s) 533
BseAI TCCGGA 1 cut(s) 260
BseBI CCWGG 1 cut(s) 701
BseCI ATCGAT 1 cut(s) 266
BseDI CCNNGG 5 cut(s) 39, 435, 836, 1013, 1071
BseGI GGATG 4 cut(s) 493, 529, 718, 958
BseJI GATNNNNATC 1 cut(s) 533
BseLI CCNNNNNNNGG 3 cut(s) 236, 410, 411
BseMI GCAATG 2 cut(s) 814, 1103
BseMII CTCAG 2 cut(s) 777, 971
BseNI ACTGG 1 cut(s) 405
BseSI GKGCMC 1 cut(s) 1012
BseX3I CGGCCG 2 cut(s) 33, 396
BseXI GCAGC 2 cut(s) 514, 1120
BseYI CCCAGC 1 cut(s) 365
BsgI GTGCAG 1 cut(s) 932
Bsh1236I CGCG 2 cut(s) 347, 401
Bsh1285I CGRYCG 5 cut(s) 36, 270, 333, 399, 425
BshFI GGCC 6 cut(s) 35, 398, 440, 596, 791, 875
BshVI ATCGAT 1 cut(s) 266
BsiEI CGRYCG 5 cut(s) 36, 270, 333, 399, 425
BsiHKAI GWGCWC 1 cut(s) 1012
BsiSI CCGG 8 cut(s) 32, 261, 392, 411, 425, 725, 881, 950
BslI CCNNNNNNNGG 3 cut(s) 236, 410, 411
BsmAI GTCTC 3 cut(s) 272, 340, 425
BsmBI CGTCTC 1 cut(s) 340
BsnI GGCC 6 cut(s) 35, 398, 440, 596, 791, 875
Bso31I GGTCTC 2 cut(s) 272, 425
Bsp119I TTCGAA 3 cut(s) 558, 587, 734
Bsp1286I GDGCHC 1 cut(s) 1012
Bsp13I TCCGGA 1 cut(s) 260
Bsp143I GATC 6 cut(s) 127, 263, 267, 330, 534, 675
BspANI GGCC 6 cut(s) 35, 398, 440, 596, 791, 875
BspCNI CTCAG 2 cut(s) 778, 972
BspDI ATCGAT 1 cut(s) 266
BspEI TCCGGA 1 cut(s) 260
BspFNI CGCG 2 cut(s) 347, 401
BspLI GGNNCC 3 cut(s) 6, 597, 723
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 3 cut(s) 135, 271, 529
BspT104I TTCGAA 3 cut(s) 558, 587, 734
BspTI CTTAAG 1 cut(s) 749
BspTNI GGTCTC 2 cut(s) 272, 425
BsrDI GCAATG 2 cut(s) 814, 1103
BsrFI RCCGGY 4 cut(s) 31, 391, 424, 880
BsrI ACTGG 1 cut(s) 405
BssAI RCCGGY 4 cut(s) 31, 391, 424, 880
BssECI CCNNGG 5 cut(s) 39, 435, 836, 1013, 1071
BssMI GATC 6 cut(s) 127, 263, 267, 330, 534, 675
BssT1I CCWWGG 1 cut(s) 1071
Bst2UI CCWGG 1 cut(s) 701
Bst4CI ACNGT 3 cut(s) 67, 322, 868
Bst6I CTCTTC 6 cut(s) 69, 75, 144, 150, 159, 165
BstAFI CTTAAG 1 cut(s) 749
BstBI TTCGAA 3 cut(s) 558, 587, 734
BstC8I GCNNGC 3 cut(s) 33, 345, 393
BstDEI CTNAG 3 cut(s) 786, 966, 980
BstDSI CCRYGG 1 cut(s) 39
BstF5I GGATG 4 cut(s) 493, 529, 718, 958
BstFNI CGCG 2 cut(s) 347, 401
BstHHI GCGC 1 cut(s) 403
BstKTI GATC 6 cut(s) 130, 266, 270, 333, 537, 678
BstMAI GTCTC 3 cut(s) 272, 340, 425
BstMBI GATC 6 cut(s) 127, 263, 267, 330, 534, 675
BstMCI CGRYCG 5 cut(s) 36, 270, 333, 399, 425
BstMWI GCNNNNNNNGC 3 cut(s) 353, 392, 745
BstNI CCWGG 1 cut(s) 701
BstSCI CCNGG 4 cut(s) 410, 699, 723, 948
BstSLI GKGCMC 1 cut(s) 1012
BstUI CGCG 2 cut(s) 347, 401
BstV1I GCAGC 2 cut(s) 514, 1120
BstV2I GAAGAC 4 cut(s) 30, 96, 258, 380
BstX2I RGATCY 1 cut(s) 127
BstYI RGATCY 1 cut(s) 127
BstZI CGGCCG 2 cut(s) 33, 396
Bsu15I ATCGAT 1 cut(s) 266
BsuI GTATCC 1 cut(s) 981
BsuRI GGCC 6 cut(s) 35, 398, 440, 596, 791, 875
BsuTUI ATCGAT 1 cut(s) 266
BtgI CCRYGG 1 cut(s) 39
BtsCI GGATG 4 cut(s) 493, 529, 718, 958
BtsIMutI CAGTG 1 cut(s) 858
BveI ACCTGC 1 cut(s) 619
Cac8I GCNNGC 3 cut(s) 33, 345, 393
CciNI GCGGCCGC 1 cut(s) 396
CfoI GCGC 1 cut(s) 403
Cfr10I RCCGGY 4 cut(s) 31, 391, 424, 880
Cfr13I GGNCC 2 cut(s) 595, 721
ClaI ATCGAT 1 cut(s) 266
CseI GACGC 1 cut(s) 882
Csp6I GTAC 1 cut(s) 697
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 2 cut(s) 743, 975
CviQI GTAC 1 cut(s) 697
DdeI CTNAG 3 cut(s) 786, 966, 980
DpnI GATC 6 cut(s) 129, 265, 269, 332, 536, 677
DpnII GATC 6 cut(s) 127, 263, 267, 330, 534, 675
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 33, 396
EagI CGGCCG 2 cut(s) 33, 396
Eam1104I CTCTTC 6 cut(s) 69, 75, 144, 150, 159, 165
EarI CTCTTC 6 cut(s) 69, 75, 144, 150, 159, 165
EciI GGCGGA 1 cut(s) 443
EclXI CGGCCG 2 cut(s) 33, 396
Eco130I CCWWGG 1 cut(s) 1071
Eco31I GGTCTC 2 cut(s) 272, 425
Eco47I GGWCC 1 cut(s) 721
Eco52I CGGCCG 2 cut(s) 33, 396
Eco57I CTGAAG 1 cut(s) 560
EcoO109I RGGNCCY 1 cut(s) 721
EcoRII CCWGG 1 cut(s) 699
EcoT14I CCWWGG 1 cut(s) 1071
ErhI CCWWGG 1 cut(s) 1071
Esp3I CGTCTC 1 cut(s) 340
FaeI CATG 2 cut(s) 746, 978
FaiI YATR 4 cut(s) 362, 551, 744, 976
FatI CATG 2 cut(s) 742, 974
FauI CCCGC 2 cut(s) 36, 352
FblI GTMKAC 1 cut(s) 69
FokI GGATG 4 cut(s) 500, 536, 725, 965
GlaI GCGC 1 cut(s) 402
GsaI CCCAGC 1 cut(s) 369
GsuI CTGGAG 1 cut(s) 726
HaeIII GGCC 6 cut(s) 35, 398, 440, 596, 791, 875
HapII CCGG 8 cut(s) 32, 261, 392, 411, 425, 725, 881, 950
HgaI GACGC 1 cut(s) 882
HhaI GCGC 1 cut(s) 403
Hin1II CATG 2 cut(s) 746, 978
Hin6I GCGC 1 cut(s) 401
HinP1I GCGC 1 cut(s) 401
HincII GTYRAC 3 cut(s) 70, 666, 856
HindII GTYRAC 3 cut(s) 70, 666, 856
HindIII AAGCTT 1 cut(s) 1139
HinfI GANTC 5 cut(s) 50, 257, 284, 798, 1178
HpaI GTTAAC 1 cut(s) 856
HpaII CCGG 8 cut(s) 32, 261, 392, 411, 425, 725, 881, 950
HphI GGTGA 1 cut(s) 310
Hpy166II GTNNAC 4 cut(s) 70, 666, 856, 1010
Hpy188III TCNNGA 7 cut(s) 217, 261, 538, 616, 679, 1135, 1200
Hpy8I GTNNAC 4 cut(s) 70, 666, 856, 1010
Hpy99I CGWCG 4 cut(s) 59, 71, 74, 482
HpyAV CCTTC 4 cut(s) 460, 535, 808, 1062
HpyCH4III ACNGT 3 cut(s) 67, 322, 868
HpyCH4V TGCA 7 cut(s) 20, 628, 768, 913, 1010, 1108, 1153
HpyF10VI GCNNNNNNNGC 3 cut(s) 353, 392, 745
HpyF3I CTNAG 3 cut(s) 786, 966, 980
Hsp92II CATG 2 cut(s) 746, 978
HspAI GCGC 1 cut(s) 401
Kpn2I TCCGGA 1 cut(s) 260
KroI GCCGGC 2 cut(s) 31, 391
KroNI GCCGGC 2 cut(s) 33, 393
KspAI GTTAAC 1 cut(s) 856
Kzo9I GATC 6 cut(s) 127, 263, 267, 330, 534, 675
LmnI GCTCC 2 cut(s) 10, 513
Lsp1109I GCAGC 2 cut(s) 514, 1120
LweI GCATC 5 cut(s) 478, 651, 880, 1038, 1140
MaeIII GTNAC 2 cut(s) 214, 922
MalI GATC 6 cut(s) 129, 265, 269, 332, 536, 677
MboI GATC 6 cut(s) 127, 263, 267, 330, 534, 675
MfeI CAATTG 2 cut(s) 650, 1003
MflI RGATCY 1 cut(s) 127
MhlI GDGCHC 1 cut(s) 1012
MluCI AATT 2 cut(s) 650, 1003
MlyI GAGTC 3 cut(s) 44, 293, 792
MmeI TCCRAC 5 cut(s) 78, 255, 336, 999, 1065
MroI TCCGGA 1 cut(s) 260
MroNI GCCGGC 2 cut(s) 31, 391
MseI TTAA 4 cut(s) 750, 855, 939, 1025
MslI CAYNNNNRTG 1 cut(s) 1118
MspCI CTTAAG 1 cut(s) 749
MspI CCGG 8 cut(s) 32, 261, 392, 411, 425, 725, 881, 950
MspR9I CCNGG 4 cut(s) 412, 701, 725, 950
MunI CAATTG 2 cut(s) 650, 1003
MvaI CCWGG 1 cut(s) 701
MvnI CGCG 2 cut(s) 347, 401
MwoI GCNNNNNNNGC 3 cut(s) 353, 392, 745
NaeI GCCGGC 2 cut(s) 33, 393
NciI CCSGG 3 cut(s) 412, 725, 950
NdeII GATC 6 cut(s) 127, 263, 267, 330, 534, 675
NgoMIV GCCGGC 2 cut(s) 31, 391
NlaIII CATG 2 cut(s) 746, 978
NlaIV GGNNCC 3 cut(s) 6, 597, 723
NmeAIII GCCGAG 1 cut(s) 861
NmuCI GTSAC 2 cut(s) 214, 922
NotI GCGGCCGC 1 cut(s) 396
NspV TTCGAA 3 cut(s) 558, 587, 734
PcsI WCGNNNNNNNCGW 2 cut(s) 63, 477
PdiI GCCGGC 2 cut(s) 33, 393
PfeI GAWTC 2 cut(s) 257, 1178
PflFI GACNNNGTC 1 cut(s) 283
Ple19I CGATCG 2 cut(s) 270, 333
PleI GAGTC 3 cut(s) 44, 292, 792
PpsI GAGTC 3 cut(s) 44, 292, 792
PpuMI RGGWCCY 1 cut(s) 721
Psp5II RGGWCCY 1 cut(s) 721
Psp6I CCWGG 1 cut(s) 699
PspFI CCCAGC 1 cut(s) 365
PspGI CCWGG 1 cut(s) 699
PspN4I GGNNCC 3 cut(s) 6, 597, 723
PspPI GGNCC 2 cut(s) 595, 721
PspPPI RGGWCCY 1 cut(s) 721
PsuI RGATCY 1 cut(s) 127
PsyI GACNNNGTC 1 cut(s) 283
PvuI CGATCG 2 cut(s) 270, 333
RsaI GTAC 1 cut(s) 698
RsaNI GTAC 1 cut(s) 697
RseI CAYNNNNRTG 1 cut(s) 1118
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 4 cut(s) 750, 855, 939, 1025
Sau3AI GATC 6 cut(s) 127, 263, 267, 330, 534, 675
Sau96I GGNCC 2 cut(s) 595, 721
SchI GAGTC 3 cut(s) 44, 293, 792
ScrFI CCNGG 4 cut(s) 412, 701, 725, 950
SduI GDGCHC 1 cut(s) 1012
SfaNI GCATC 5 cut(s) 478, 651, 880, 1038, 1140
SfuI TTCGAA 3 cut(s) 558, 587, 734
SgrDI CGTCGACG 1 cut(s) 68
SinI GGWCC 1 cut(s) 721
SmiMI CAYNNNNRTG 1 cut(s) 1118
SmlI CTYRAG 2 cut(s) 605, 749
SmoI CTYRAG 2 cut(s) 605, 749
Sse9I AATT 2 cut(s) 650, 1003
StyD4I CCNGG 4 cut(s) 410, 699, 723, 948
StyI CCWWGG 1 cut(s) 1071
TaaI ACNGT 3 cut(s) 67, 322, 868
TaqII GACCGA 2 cut(s) 296, 449
TasI AATT 2 cut(s) 650, 1003
TauI GCSGC 8 cut(s) 38, 212, 359, 398, 401, 477, 581, 835
TfiI GAWTC 2 cut(s) 257, 1178
Tru1I TTAA 4 cut(s) 750, 855, 939, 1025
Tru9I TTAA 4 cut(s) 750, 855, 939, 1025
TscAI CASTG 1 cut(s) 865
TseFI GTSAC 2 cut(s) 214, 922
TseI GCWGC 2 cut(s) 502, 1108
Tsp45I GTSAC 2 cut(s) 214, 922
TspDTI ATGAA 3 cut(s) 75, 601, 1199
TspRI CASTG 1 cut(s) 865
Tth111I GACNNNGTC 1 cut(s) 283
Vha464I CTTAAG 1 cut(s) 749
VneI GTGCAC 1 cut(s) 1008
VpaK11BI GGWCC 1 cut(s) 721
XmiI GTMKAC 1 cut(s) 69
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.