Rh2DG570400

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0008960
Physical Location & Seq
Forward (+)
80084841 .. 80086124
1284 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG570400.1

Sequence Viewer

Length: 1284 bp
ATGGCTCCCAAGCGCCTCCTCCAAGACCCTCCCGCTGCTTCATCAATGGAGGAAGACTCCGACGCAGAGACCCTGGATGAAGAACAAGAAGAAGAAGAAGCAGAAGAGGAACAAAACGACGCCGTTCAAGAAAAATCTGATGAGGAAGAAGAGGAGGAGGAAGAGGAAGAAGAGGAGGAGGAAGAAGAAGATAATGAGGAAGAGGAGAATGTGAATGCTAAGAAGCCCGTTGTGATTCCCTCATCGTCAGTTGTTCCAAACCCTCAATCGGAATCGGCCTCCTCCGAAGACGACGACGCCGCCGCCGGGTCGGAGACCGAGTCCGACGACATCAACCACTCTCCATCCGCTTCCGATTTCACCGTCAAGCCCATTGTCTCCAAGTCCGTGAACGACTCCTCCTCCGCCAAGCCCACCAAGAAGCCCGCTCCGGCTCCGGCGAAGCCCGCTCCCGGGTCGAAGCGCCCGGCCGAGAGCGATCCGACTCCGAAAGACTCGAAGAAGAAGAAGAAGGGTGCCAACGGCGGAGACGATGAGGACACCAAAAAGGGTCGGCTTTGGGGCGAGGACGATGAGATTGCGATCCTGAAGGGGATGATAGACTTCAAGGCCAAGAAGGGGATCGACCCGTCTAGTAACATGGGCGCGTTCCATGAGTTCTTGAAGAGAAGCTTGAAGGCTGACGTCAGCAAGAACCAGTTGATGGATAAGACCAGGAGGTTGAAGAAGAAGTACCATACGAATGCTGAGAAGGGCGAGAATGGGGAGGGCCCGGTGTTTTCGAAGGCGCATGAGTTGAAGTCGTTTGATTTGTCGAAGAAGATTTGGGGTGCTGAGGCTAATGGGGGAGATGATAGTGCAAAGAACAGTAGGAAGAAGCCGAGGAAGAGTGCCAAGGCTAACAACAACAACAACTCTTCTACAGTCTTGGCATTGCCTGTTTCGGATGCTGCGGCTGAGAGGAATGTGGAGAAGAAAGTAAAAGTGGAGGAGATGAATGTTGCTAATGGCGGAGTTAAGGCTGCTGAGTCCGATGACTTTTGGTCCAAGTACCCATCGCTGAGTGATTCTTTGCAGCTTGGAAATTGCTCATCGAGGTGGGAGGGTTTGGAGAGAGTTATGATTCAGAAAATGCCCTCAATTGGGAGCTCAAAGGCTAAAGAGTTGGATGATAGGTGGAGGAAGTTTCAGGTCTTAGAGATGGAGTTATCTGTCAAGAAGCTTGATTTGATGCATGAACAAGCTAAGCTTTTATTGGATGGGATAAAGTCGCGGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

427

Amino Acids

47.0

Weight (kDa)

4.84

Isoelectric Point (pI)

63.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 185 - 277 6.1e-33 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 687
AccB1I GGYRCC 1 cut(s) 515
AccB7I CCANNNNNTGG 1 cut(s) 703
AccBSI CCGCTC 2 cut(s) 428, 449
AccII CGCG 2 cut(s) 647, 1273
AclWI GGATC 3 cut(s) 473, 577, 629
AcoI YGGCCR 1 cut(s) 468
AcuI CTGAAG 1 cut(s) 608
AcyI GRCGYC 3 cut(s) 120, 297, 684
AfaI GTAC 2 cut(s) 734, 1052
AfiI CCNNNNNNNGG 8 cut(s) 268, 306, 452, 453, 618, 703, 1142, 1143
AgsI TTSAA 6 cut(s) 128, 607, 664, 676, 724, 799
AhdI GACNNNNNGTC 1 cut(s) 1042
AjnI CCWGG 2 cut(s) 72, 713
AloI GAACNNNNNNTCC 2 cut(s) 383, 415
AluBI AGCT 6 cut(s) 672, 1078, 1149, 1222, 1244, 1249
AluI AGCT 6 cut(s) 672, 1078, 1149, 1222, 1244, 1249
Alw21I GWGCWC 1 cut(s) 1151
Alw26I GTCTC 4 cut(s) 62, 308, 382, 522
AlwI GGATC 3 cut(s) 473, 577, 629
Ama87I CYCGRG 1 cut(s) 452
AoxI GGCC 4 cut(s) 276, 468, 609, 769
ApaI GGGCCC 1 cut(s) 773
ApeKI GCWGC 4 cut(s) 35, 950, 1022, 1075
AspLEI GCGC 4 cut(s) 15, 465, 647, 790
AspS9I GGNCC 3 cut(s) 769, 770, 1044
AsuC2I CCSGG 5 cut(s) 307, 453, 454, 467, 773
AsuHPI GGTGA 1 cut(s) 352
AsuII TTCGAA 1 cut(s) 782
AvaI CYCGRG 1 cut(s) 452
AvaII GGWCC 1 cut(s) 1044
BaeGI GKGCMC 1 cut(s) 773
BanI GGYRCC 1 cut(s) 515
BanII GRGCYC 2 cut(s) 773, 1151
BarI GAAGNNNNNNTAC 2 cut(s) 716, 748
BbsI GAAGAC 2 cut(s) 60, 294
Bbv12I GWGCWC 1 cut(s) 1151
BbvCI CCTCAGC 1 cut(s) 834
BbvI GCAGC 4 cut(s) 22, 937, 1009, 1087
BccI CCATC 5 cut(s) 352, 697, 1063, 1195, 1253
BceAI ACGGC 2 cut(s) 107, 538
BcgI CGANNNNNNTGC 2 cut(s) 560, 594
BciT130I CCWGG 2 cut(s) 74, 715
BcnI CCSGG 5 cut(s) 307, 453, 454, 467, 773
BcoDI GTCTC 4 cut(s) 62, 308, 382, 522
BfaI CTAG 1 cut(s) 633
BfmI CTRYAG 1 cut(s) 921
BfoI RGCGCY 2 cut(s) 16, 466
BisI GCNGC 7 cut(s) 36, 300, 303, 951, 954, 1023, 1076
BlpI GCTNAGC 1 cut(s) 1245
BlsI GCNGC 7 cut(s) 37, 301, 304, 952, 955, 1024, 1077
Bme1390I CCNGG 7 cut(s) 74, 307, 453, 454, 467, 715, 773
Bme18I GGWCC 1 cut(s) 1044
BmeRI GACNNNNNGTC 1 cut(s) 1042
BmeT110I CYCGRG 1 cut(s) 452
BmgT120I GGNCC 3 cut(s) 769, 770, 1044
BmiI GGNNCC 4 cut(s) 6, 435, 517, 771
BmrFI CCNGG 7 cut(s) 74, 307, 453, 454, 467, 715, 773
BmsI GCATC 2 cut(s) 937, 1221
BpiI GAAGAC 2 cut(s) 60, 294
BplI GAGNNNNNCTC 2 cut(s) 41, 73
Bpu10I CCTNAGC 1 cut(s) 834
Bpu1102I GCTNAGC 1 cut(s) 1245
Bpu14I TTCGAA 1 cut(s) 782
BpuMI CCSGG 5 cut(s) 307, 453, 454, 467, 773
BsaBI GATNNNNATC 1 cut(s) 581
BsaHI GRCGYC 3 cut(s) 120, 297, 684
BsaI GGTCTC 2 cut(s) 62, 308
BsaJI CCNNGG 4 cut(s) 72, 452, 881, 894
BsaXI ACNNNNNCTCC 6 cut(s) 305, 335, 383, 386, 413, 416
Bsc4I CCNNNNNNNGG 8 cut(s) 268, 306, 452, 453, 618, 703, 1142, 1143
Bse1I ACTGG 1 cut(s) 697
Bse3DI GCAATG 1 cut(s) 932
Bse8I GATNNNNATC 1 cut(s) 581
BseBI CCWGG 2 cut(s) 74, 715
BseDI CCNNGG 4 cut(s) 72, 452, 881, 894
BseGI GGATG 6 cut(s) 82, 344, 600, 952, 1174, 1264
BseJI GATNNNNATC 1 cut(s) 581
BseLI CCNNNNNNNGG 8 cut(s) 268, 306, 452, 453, 618, 703, 1142, 1143
BseMI GCAATG 1 cut(s) 932
BseMII CTCAG 5 cut(s) 738, 825, 948, 1017, 1052
BseNI ACTGG 1 cut(s) 697
BseSI GKGCMC 1 cut(s) 773
BseX3I CGGCCG 1 cut(s) 468
BseXI GCAGC 4 cut(s) 22, 937, 1009, 1087
Bsh1236I CGCG 2 cut(s) 647, 1273
Bsh1285I CGRYCG 1 cut(s) 471
BshFI GGCC 4 cut(s) 278, 470, 611, 771
BshNI GGYRCC 1 cut(s) 515
BsiEI CGRYCG 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 1151
BsiHKCI CYCGRG 1 cut(s) 452
BsiSI CCGG 6 cut(s) 306, 431, 437, 453, 467, 773
BslI CCNNNNNNNGG 8 cut(s) 268, 306, 452, 453, 618, 703, 1142, 1143
BsmAI GTCTC 4 cut(s) 62, 308, 382, 522
BsmBI CGTCTC 1 cut(s) 522
BsmI GAATGC 2 cut(s) 220, 748
BsnI GGCC 4 cut(s) 278, 470, 611, 771
Bso31I GGTCTC 2 cut(s) 62, 308
BsoBI CYCGRG 1 cut(s) 452
Bsp119I TTCGAA 1 cut(s) 782
Bsp120I GGGCCC 1 cut(s) 769
Bsp1286I GDGCHC 2 cut(s) 773, 1151
Bsp143I GATC 3 cut(s) 478, 582, 621
Bsp1720I GCTNAGC 1 cut(s) 1245
BspANI GGCC 4 cut(s) 278, 470, 611, 771
BspCNI CTCAG 5 cut(s) 739, 826, 949, 1018, 1053
BspFNI CGCG 2 cut(s) 647, 1273
BspLI GGNNCC 4 cut(s) 6, 435, 517, 771
BspPI GGATC 3 cut(s) 473, 577, 629
BspT104I TTCGAA 1 cut(s) 782
BspT107I GGYRCC 1 cut(s) 515
BspTNI GGTCTC 2 cut(s) 62, 308
BsrBI CCGCTC 2 cut(s) 428, 449
BsrDI GCAATG 1 cut(s) 932
BsrI ACTGG 1 cut(s) 697
BssECI CCNNGG 4 cut(s) 72, 452, 881, 894
BssMI GATC 3 cut(s) 478, 582, 621
BssNI GRCGYC 3 cut(s) 120, 297, 684
BssT1I CCWWGG 1 cut(s) 894
Bst2UI CCWGG 2 cut(s) 74, 715
Bst4CI ACNGT 3 cut(s) 364, 869, 925
Bst6I CTCTTC 8 cut(s) 99, 144, 156, 165, 195, 659, 881, 922
BstACI GRCGYC 3 cut(s) 120, 297, 684
BstBI TTCGAA 1 cut(s) 782
BstC8I GCNNGC 2 cut(s) 426, 447
BstDEI CTNAG 8 cut(s) 219, 747, 834, 957, 1026, 1061, 1195, 1245
BstF5I GGATG 6 cut(s) 82, 344, 600, 952, 1174, 1264
BstFNI CGCG 2 cut(s) 647, 1273
BstH2I RGCGCY 2 cut(s) 16, 466
BstHHI GCGC 4 cut(s) 15, 465, 647, 790
BstKTI GATC 3 cut(s) 481, 585, 624
BstMAI GTCTC 4 cut(s) 62, 308, 382, 522
BstMBI GATC 3 cut(s) 478, 582, 621
BstMCI CGRYCG 1 cut(s) 471
BstMWI GCNNNNNNNGC 1 cut(s) 446
BstNI CCWGG 2 cut(s) 74, 715
BstSCI CCNGG 7 cut(s) 72, 305, 451, 452, 465, 713, 771
BstSFI CTRYAG 1 cut(s) 921
BstSLI GKGCMC 1 cut(s) 773
BstUI CGCG 2 cut(s) 647, 1273
BstV1I GCAGC 4 cut(s) 22, 937, 1009, 1087
BstV2I GAAGAC 2 cut(s) 60, 294
BstZI CGGCCG 1 cut(s) 468
BsuRI GGCC 4 cut(s) 278, 470, 611, 771
BtgZI GCGATG 1 cut(s) 1041
BtsCI GGATG 6 cut(s) 82, 344, 600, 952, 1174, 1264
Cac8I GCNNGC 2 cut(s) 426, 447
CfoI GCGC 4 cut(s) 15, 465, 647, 790
Cfr13I GGNCC 3 cut(s) 769, 770, 1044
Cfr9I CCCGGG 1 cut(s) 452
CseI GACGC 3 cut(s) 71, 128, 305
Csp6I GTAC 2 cut(s) 733, 1051
CviAII CATG 4 cut(s) 640, 653, 791, 1235
CviQI GTAC 2 cut(s) 733, 1051
DdeI CTNAG 8 cut(s) 219, 747, 834, 957, 1026, 1061, 1195, 1245
DpnI GATC 3 cut(s) 480, 584, 623
DpnII GATC 3 cut(s) 478, 582, 621
DriI GACNNNNNGTC 1 cut(s) 1042
EaeI YGGCCR 1 cut(s) 468
EagI CGGCCG 1 cut(s) 468
Eam1104I CTCTTC 8 cut(s) 99, 144, 156, 165, 195, 659, 881, 922
Eam1105I GACNNNNNGTC 1 cut(s) 1042
EarI CTCTTC 8 cut(s) 99, 144, 156, 165, 195, 659, 881, 922
EciI GGCGGA 3 cut(s) 394, 540, 1026
Ecl136II GAGCTC 1 cut(s) 1149
EclXI CGGCCG 1 cut(s) 468
Eco130I CCWWGG 1 cut(s) 894
Eco24I GRGCYC 2 cut(s) 773, 1151
Eco31I GGTCTC 2 cut(s) 62, 308
Eco47I GGWCC 1 cut(s) 1044
Eco52I CGGCCG 1 cut(s) 468
Eco53kI GAGCTC 1 cut(s) 1149
Eco57I CTGAAG 1 cut(s) 608
Eco88I CYCGRG 1 cut(s) 452
EcoICRI GAGCTC 1 cut(s) 1149
EcoO109I RGGNCCY 1 cut(s) 769
EcoRII CCWGG 2 cut(s) 72, 713
EcoT14I CCWWGG 1 cut(s) 894
EcoT22I ATGCAT 1 cut(s) 1236
EcoT38I GRGCYC 2 cut(s) 773, 1151
ErhI CCWWGG 1 cut(s) 894
Esp3I CGTCTC 1 cut(s) 522
FaeI CATG 4 cut(s) 643, 656, 794, 1238
FaiI YATR 6 cut(s) 641, 654, 738, 792, 1121, 1236
FalI AAGNNNNNCTT 4 cut(s) 656, 688, 1233, 1265
FatI CATG 4 cut(s) 639, 652, 790, 1234
FauI CCCGC 3 cut(s) 40, 433, 454
Fnu4HI GCNGC 7 cut(s) 36, 300, 303, 951, 954, 1023, 1076
FokI GGATG 6 cut(s) 89, 331, 607, 959, 1181, 1271
FriOI GRGCYC 2 cut(s) 773, 1151
Fsp4HI GCNGC 7 cut(s) 36, 300, 303, 951, 954, 1023, 1076
FspBI CTAG 1 cut(s) 633
GlaI GCGC 4 cut(s) 14, 464, 646, 789
GluI GCNGC 7 cut(s) 36, 300, 303, 951, 954, 1023, 1076
HaeII RGCGCY 2 cut(s) 16, 466
HaeIII GGCC 4 cut(s) 278, 470, 611, 771
HapII CCGG 6 cut(s) 306, 431, 437, 453, 467, 773
HgaI GACGC 3 cut(s) 71, 128, 305
HhaI GCGC 4 cut(s) 15, 465, 647, 790
Hin1I GRCGYC 3 cut(s) 120, 297, 684
Hin1II CATG 4 cut(s) 643, 656, 794, 1238
Hin6I GCGC 4 cut(s) 13, 463, 645, 788
HinP1I GCGC 4 cut(s) 13, 463, 645, 788
HindIII AAGCTT 3 cut(s) 670, 1220, 1247
HpaII CCGG 6 cut(s) 306, 431, 437, 453, 467, 773
HphI GGTGA 1 cut(s) 352
Hpy166II GTNNAC 1 cut(s) 391
Hpy188III TCNNGA 4 cut(s) 128, 586, 661, 1216
Hpy8I GTNNAC 1 cut(s) 391
Hpy99I CGWCG 5 cut(s) 65, 122, 296, 299, 329
HpyAV CCTTC 7 cut(s) 505, 583, 610, 670, 745, 778, 1270
HpyCH4III ACNGT 3 cut(s) 364, 869, 925
HpyCH4IV ACGT 1 cut(s) 684
HpyCH4V TGCA 3 cut(s) 860, 1075, 1234
HpyF10VI GCNNNNNNNGC 1 cut(s) 446
HpyF3I CTNAG 8 cut(s) 219, 747, 834, 957, 1026, 1061, 1195, 1245
HpySE526I ACGT 1 cut(s) 684
Hsp92I GRCGYC 3 cut(s) 120, 297, 684
Hsp92II CATG 4 cut(s) 643, 656, 794, 1238
HspAI GCGC 4 cut(s) 13, 463, 645, 788
Kzo9I GATC 3 cut(s) 478, 582, 621
LmnI GCTCC 5 cut(s) 10, 433, 439, 454, 1146
Lsp1109I GCAGC 4 cut(s) 22, 937, 1009, 1087
LweI GCATC 2 cut(s) 937, 1221
MaeI CTAG 1 cut(s) 633
MaeII ACGT 1 cut(s) 684
MaeIII GTNAC 1 cut(s) 635
MalI GATC 3 cut(s) 480, 584, 623
MbiI CCGCTC 2 cut(s) 428, 449
MboI GATC 3 cut(s) 478, 582, 621
MfeI CAATTG 1 cut(s) 1140
MhlI GDGCHC 2 cut(s) 773, 1151
MluCI AATT 2 cut(s) 1084, 1140
MlyI GAGTC 6 cut(s) 50, 329, 389, 478, 488, 1037
MmeI TCCRAC 5 cut(s) 84, 291, 348, 506, 1146
Mph1103I ATGCAT 1 cut(s) 1236
MseI TTAA 1 cut(s) 1017
MslI CAYNNNNRTG 2 cut(s) 741, 1096
MspA1I CMGCKG 1 cut(s) 35
MspI CCGG 6 cut(s) 306, 431, 437, 453, 467, 773
MspR9I CCNGG 7 cut(s) 74, 307, 453, 454, 467, 715, 773
MunI CAATTG 1 cut(s) 1140
Mva1269I GAATGC 2 cut(s) 220, 748
MvaI CCWGG 2 cut(s) 74, 715
MvnI CGCG 2 cut(s) 647, 1273
MwoI GCNNNNNNNGC 1 cut(s) 446
NciI CCSGG 5 cut(s) 307, 453, 454, 467, 773
NdeII GATC 3 cut(s) 478, 582, 621
NlaIII CATG 4 cut(s) 643, 656, 794, 1238
NlaIV GGNNCC 4 cut(s) 6, 435, 517, 771
NmeAIII GCCGAG 2 cut(s) 496, 906
NsiI ATGCAT 1 cut(s) 1236
NspV TTCGAA 1 cut(s) 782
PcsI WCGNNNNNNNCGW 1 cut(s) 528
PctI GAATGC 2 cut(s) 220, 748
PfeI GAWTC 4 cut(s) 235, 272, 1067, 1123
PflFI GACNNNGTC 1 cut(s) 319
PflMI CCANNNNNTGG 1 cut(s) 703
PkrI GCNGC 7 cut(s) 37, 301, 304, 952, 955, 1024, 1077
PleI GAGTC 6 cut(s) 50, 328, 389, 478, 488, 1036
PpsI GAGTC 6 cut(s) 50, 328, 389, 478, 488, 1036
Psp124BI GAGCTC 1 cut(s) 1151
Psp6I CCWGG 2 cut(s) 72, 713
PspGI CCWGG 2 cut(s) 72, 713
PspN4I GGNNCC 4 cut(s) 6, 435, 517, 771
PspOMI GGGCCC 1 cut(s) 769
PspPI GGNCC 3 cut(s) 769, 770, 1044
PsyI GACNNNGTC 1 cut(s) 319
RsaI GTAC 2 cut(s) 734, 1052
RsaNI GTAC 2 cut(s) 733, 1051
RseI CAYNNNNRTG 2 cut(s) 741, 1096
SacI GAGCTC 1 cut(s) 1151
SaqAI TTAA 1 cut(s) 1017
SatI GCNGC 7 cut(s) 36, 300, 303, 951, 954, 1023, 1076
Sau3AI GATC 3 cut(s) 478, 582, 621
Sau96I GGNCC 3 cut(s) 769, 770, 1044
SchI GAGTC 6 cut(s) 50, 329, 389, 478, 488, 1037
ScrFI CCNGG 7 cut(s) 74, 307, 453, 454, 467, 715, 773
SduI GDGCHC 2 cut(s) 773, 1151
SfaNI GCATC 2 cut(s) 937, 1221
SfcI CTRYAG 1 cut(s) 921
SfuI TTCGAA 1 cut(s) 782
SinI GGWCC 1 cut(s) 1044
SmaI CCCGGG 1 cut(s) 454
SmiMI CAYNNNNRTG 2 cut(s) 741, 1096
Sse9I AATT 2 cut(s) 1084, 1140
SspMI CTAG 1 cut(s) 633
SstI GAGCTC 1 cut(s) 1151
StyD4I CCNGG 7 cut(s) 72, 305, 451, 452, 465, 713, 771
StyI CCWWGG 1 cut(s) 894
TaaI ACNGT 3 cut(s) 364, 869, 925
TaiI ACGT 1 cut(s) 687
TaqI TCGA 6 cut(s) 458, 497, 624, 782, 815, 1094
TaqII GACCGA 1 cut(s) 332
TasI AATT 2 cut(s) 1084, 1140
TauI GCSGC 3 cut(s) 302, 305, 956
TfiI GAWTC 4 cut(s) 235, 272, 1067, 1123
Tru1I TTAA 1 cut(s) 1017
Tru9I TTAA 1 cut(s) 1017
TseI GCWGC 4 cut(s) 35, 950, 1022, 1075
TspDTI ATGAA 4 cut(s) 30, 93, 1010, 1251
TspGWI ACGGA 1 cut(s) 376
TspMI CCCGGG 1 cut(s) 452
Tth111I GACNNNGTC 1 cut(s) 319
Van91I CCANNNNNTGG 1 cut(s) 703
VpaK11BI GGWCC 1 cut(s) 1044
XmaI CCCGGG 1 cut(s) 452
XspI CTAG 1 cut(s) 633
ZraI GACGTC 1 cut(s) 685
Zsp2I ATGCAT 1 cut(s) 1236
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.