MD09G1103400.v1.1

Mediator-associated protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0008960
Physical Location & Seq
Reverse (-)
7573796 .. 7575001
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1103400.v1.1.491

Sequence Viewer

Length: 1206 bp
ATGGCTCCGAAGAAACTTGTAGAAGACCCGCCGGCCGCCGTGATGGAGGAATCCGATGATGAAACCGTCGACGAAGAGGAAGAGGAAGGGGAAGAAGACGACGCCGTTGAAGTCAACAAAGCAAAATCTGTGGATCTCAATGATGATGATTTAGAAGAGGAAGAGGAAGAGGAAGAGGAGGAGGAAGAAGAAGAGGAGGAAGAAAAGCCCTCTGCGGTGGTCACGAAGTCAAATCCCCAATCGGGTTCTGCCTCCGAAGACGATTCCGGATCCGGGTCGGAGACCGAGTCCGATAACCACTCGCCGTCAGCTTCCGACTTCACCGTCAAGCCGATCGTCTCCAAGCCCGCAAACAGCGCTGTTATGCCCAGCAAGAAGACTGCTTCGGCGAAGCCTACGGCCGCGCCCAGTTCCGGGTCGAAGCGACCGGCGGAGACCGAGCCCAGCCTGAAAAACTCCAAGAAGAAGAAGGTCGGCGGCGACGATGAGGATGCGAAAAAGGGGCAGCGTTTTTGGAGCGAGGACGATGAGATAACGATCCTGAAGGGCATTATAGAGTTCGAAAGCAAGAAAGGTGTGGCGGCGAGTTCGAATGTGGCTCCATTCCTTGAGTTCATCAAGAATGATTTGCAGGTCGATTTGAGCACCGACCAATTGGCTGAGAAGTTGACTGATAAGATCAGGAGATTGAAGAAGAAGTACCAGGCTGGAGAGGATGGCGAGGACCCGGTGTTTTCGAAGCCCCATGAGCTTAAGTGCTTTGAACTGTGCAAAAAGATTTGGGGTTCTGAGGCCAATGGACTCGATAGCAATGTGAAGGGCGGTAAGAGGAAGCCGCCGAGGAAGAATAATAAGGTTAACAACGATACGGTTCTGGCCTTGCCGGTTTCAGACGTGTCTGTGAAGAAAGAGGTGCAGAATAATGTGACTAATAGAGGAGTTAAGGCTGACCCGGATGACTTTTGGTCTAAGTATCCATGTCTGAGTGGTTCTTTGAGGACGGGCAATTGTGCACCGAGGTCGGATTTAACTAGACTTTTGACTGAGAAGATGCCTTCTTTGGTTGGGAGTTCCAAGGCTAAAGAGTTGGAGGAGAGGTGGAGGGCATTGCAGCTCATTGAAATGGATTTGTCTGTCAAGAAGCTTGATTTGATGCAGGAACAGACTAAACTTGTATTGGATTCGATGAAGTCTTTGAAGGATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF573

Protein Analysis

402

Amino Acids

44.18

Weight (kDa)

4.7

Isoelectric Point (pI)

57.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GeBP-like_DBD PF04504 169 - 262 3e-27 Glabrous-enhancer-binding protein-like family, DBD domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 323
Acc36I ACCTGC 1 cut(s) 622
AccI GTMKAC 1 cut(s) 69
AccII CGCG 1 cut(s) 404
AccIII TCCGGA 1 cut(s) 266
AclWI GGATC 4 cut(s) 141, 264, 277, 532
AcoI YGGCCR 2 cut(s) 33, 399
AcuI CTGAAG 1 cut(s) 563
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 1 cut(s) 701
AfeI AGCGCT 1 cut(s) 358
AfiI CCNNNNNNNGG 4 cut(s) 242, 273, 413, 414
AflII CTTAAG 1 cut(s) 752
AflIII ACRYGT 1 cut(s) 894
AgsI TTSAA 5 cut(s) 110, 691, 764, 1121, 1198
AhdI GACNNNNNGTC 1 cut(s) 964
AjiI CACGTC 1 cut(s) 895
AjnI CCWGG 1 cut(s) 702
AluBI AGCT 4 cut(s) 311, 751, 1114, 1144
AluI AGCT 4 cut(s) 311, 751, 1114, 1144
Alw21I GWGCWC 2 cut(s) 647, 1015
Alw26I GTCTC 3 cut(s) 275, 343, 428
Alw44I GTGCAC 1 cut(s) 1011
AlwI GGATC 4 cut(s) 141, 264, 277, 532
Aor13HI TCCGGA 1 cut(s) 266
Aor51HI AGCGCT 1 cut(s) 358
AoxI GGCC 4 cut(s) 33, 399, 792, 876
ApaLI GTGCAC 1 cut(s) 1011
ApeKI GCWGC 2 cut(s) 505, 1111
AspLEI GCGC 2 cut(s) 359, 406
AspS9I GGNCC 1 cut(s) 724
AsuC2I CCSGG 4 cut(s) 274, 415, 728, 953
AsuHPI GGTGA 1 cut(s) 313
AsuII TTCGAA 3 cut(s) 561, 590, 737
AvaII GGWCC 1 cut(s) 724
BaeGI GKGCMC 1 cut(s) 1015
BamHI GGATCC 1 cut(s) 269
BanII GRGCYC 1 cut(s) 444
BarI GAAGNNNNNNTAC 2 cut(s) 683, 715
BbsI GAAGAC 4 cut(s) 30, 102, 264, 383
Bbv12I GWGCWC 2 cut(s) 647, 1015
BbvI GCAGC 2 cut(s) 517, 1123
BccI CCATC 2 cut(s) 37, 710
BceAI ACGGC 4 cut(s) 23, 89, 289, 414
BcgI CGANNNNNNTGC 4 cut(s) 473, 507, 1002, 1036
BciT130I CCWGG 1 cut(s) 704
BciVI GTATCC 1 cut(s) 984
BcnI CCSGG 4 cut(s) 274, 415, 728, 953
BcoDI GTCTC 3 cut(s) 275, 343, 428
BfaI CTAG 1 cut(s) 1032
BfoI RGCGCY 1 cut(s) 360
BfrI CTTAAG 1 cut(s) 752
BfuAI ACCTGC 1 cut(s) 622
BfuI GTATCC 1 cut(s) 984
BisI GCNGC 7 cut(s) 36, 402, 478, 506, 582, 836, 1112
BlsI GCNGC 7 cut(s) 37, 403, 479, 507, 583, 837, 1113
Bme1390I CCNGG 5 cut(s) 274, 415, 704, 728, 953
Bme18I GGWCC 1 cut(s) 724
BmeRI GACNNNNNGTC 1 cut(s) 964
BmgBI CACGTC 1 cut(s) 895
BmgT120I GGNCC 1 cut(s) 724
BmiI GGNNCC 4 cut(s) 6, 271, 600, 726
BmrFI CCNGG 5 cut(s) 274, 415, 704, 728, 953
BmrI ACTGGG 1 cut(s) 402
BmsI GCATC 3 cut(s) 481, 1041, 1143
BmuI ACTGGG 1 cut(s) 402
BpiI GAAGAC 4 cut(s) 30, 102, 264, 383
BpmI CTGGAG 1 cut(s) 729
Bpu14I TTCGAA 3 cut(s) 561, 590, 737
BpuEI CTTGAG 1 cut(s) 629
BpuMI CCSGG 4 cut(s) 274, 415, 728, 953
BsaBI GATNNNNATC 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 102
BsaI GGTCTC 2 cut(s) 275, 428
BsaJI CCNNGG 3 cut(s) 839, 1016, 1074
BsaWI WCCGGW 1 cut(s) 266
BsaXI ACNNNNNCTCC 4 cut(s) 272, 302, 1082, 1112
Bsc4I CCNNNNNNNGG 4 cut(s) 242, 273, 413, 414
Bse118I RCCGGY 3 cut(s) 31, 427, 883
Bse1I ACTGG 1 cut(s) 408
Bse3DI GCAATG 2 cut(s) 817, 1106
Bse8I GATNNNNATC 1 cut(s) 536
BseAI TCCGGA 1 cut(s) 266
BseBI CCWGG 1 cut(s) 704
BseDI CCNNGG 3 cut(s) 839, 1016, 1074
BseGI GGATG 3 cut(s) 496, 721, 961
BseJI GATNNNNATC 1 cut(s) 536
BseLI CCNNNNNNNGG 4 cut(s) 242, 273, 413, 414
BseMI GCAATG 2 cut(s) 817, 1106
BseMII CTCAG 4 cut(s) 651, 780, 974, 1035
BseNI ACTGG 1 cut(s) 408
BseRI GAGGAG 5 cut(s) 191, 194, 209, 951, 1106
BseSI GKGCMC 1 cut(s) 1015
BseX3I CGGCCG 2 cut(s) 33, 399
BseXI GCAGC 2 cut(s) 517, 1123
BseYI CCCAGC 2 cut(s) 368, 443
BsgI GTGCAG 1 cut(s) 935
Bsh1236I CGCG 1 cut(s) 404
Bsh1285I CGRYCG 4 cut(s) 36, 336, 402, 428
BshFI GGCC 4 cut(s) 35, 401, 794, 878
BsiEI CGRYCG 4 cut(s) 36, 336, 402, 428
BsiHKAI GWGCWC 2 cut(s) 647, 1015
BsiSI CCGG 8 cut(s) 32, 267, 273, 414, 428, 728, 884, 953
BslI CCNNNNNNNGG 4 cut(s) 242, 273, 413, 414
BsmAI GTCTC 3 cut(s) 275, 343, 428
BsmBI CGTCTC 1 cut(s) 343
BsnI GGCC 4 cut(s) 35, 401, 794, 878
Bso31I GGTCTC 2 cut(s) 275, 428
Bsp119I TTCGAA 3 cut(s) 561, 590, 737
Bsp1286I GDGCHC 3 cut(s) 444, 647, 1015
Bsp13I TCCGGA 1 cut(s) 266
Bsp143I GATC 5 cut(s) 133, 269, 333, 537, 678
BspANI GGCC 4 cut(s) 35, 401, 794, 878
BspCNI CTCAG 4 cut(s) 652, 781, 975, 1036
BspEI TCCGGA 1 cut(s) 266
BspFNI CGCG 1 cut(s) 404
BspLI GGNNCC 4 cut(s) 6, 271, 600, 726
BspMI ACCTGC 1 cut(s) 622
BspPI GGATC 4 cut(s) 141, 264, 277, 532
BspT104I TTCGAA 3 cut(s) 561, 590, 737
BspTI CTTAAG 1 cut(s) 752
BspTNI GGTCTC 2 cut(s) 275, 428
BsrDI GCAATG 2 cut(s) 817, 1106
BsrFI RCCGGY 3 cut(s) 31, 427, 883
BsrI ACTGG 1 cut(s) 408
BssAI RCCGGY 3 cut(s) 31, 427, 883
BssECI CCNNGG 3 cut(s) 839, 1016, 1074
BssMI GATC 5 cut(s) 133, 269, 333, 537, 678
BssNI GRCGYC 1 cut(s) 102
BssT1I CCWWGG 1 cut(s) 1074
Bst2UI CCWGG 1 cut(s) 704
Bst4CI ACNGT 4 cut(s) 67, 325, 768, 871
Bst6I CTCTTC 7 cut(s) 69, 75, 150, 156, 162, 168, 186
BstACI GRCGYC 1 cut(s) 102
BstAFI CTTAAG 1 cut(s) 752
BstBI TTCGAA 3 cut(s) 561, 590, 737
BstC8I GCNNGC 2 cut(s) 33, 348
BstDEI CTNAG 5 cut(s) 660, 789, 969, 983, 1044
BstF5I GGATG 3 cut(s) 496, 721, 961
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 360
BstHHI GCGC 2 cut(s) 359, 406
BstKTI GATC 5 cut(s) 136, 272, 336, 540, 681
BstMAI GTCTC 3 cut(s) 275, 343, 428
BstMBI GATC 5 cut(s) 133, 269, 333, 537, 678
BstMCI CGRYCG 4 cut(s) 36, 336, 402, 428
BstMWI GCNNNNNNNGC 2 cut(s) 356, 748
BstNI CCWGG 1 cut(s) 704
BstSCI CCNGG 5 cut(s) 272, 413, 702, 726, 951
BstSLI GKGCMC 1 cut(s) 1015
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 2 cut(s) 517, 1123
BstV2I GAAGAC 4 cut(s) 30, 102, 264, 383
BstX2I RGATCY 2 cut(s) 133, 269
BstYI RGATCY 2 cut(s) 133, 269
BstZI CGGCCG 2 cut(s) 33, 399
BsuI GTATCC 1 cut(s) 984
BsuRI GGCC 4 cut(s) 35, 401, 794, 878
BtrI CACGTC 1 cut(s) 895
BtsCI GGATG 3 cut(s) 496, 721, 961
BveI ACCTGC 1 cut(s) 622
Cac8I GCNNGC 2 cut(s) 33, 348
CfoI GCGC 2 cut(s) 359, 406
Cfr10I RCCGGY 3 cut(s) 31, 427, 883
Cfr13I GGNCC 1 cut(s) 724
CseI GACGC 1 cut(s) 110
Csp6I GTAC 1 cut(s) 700
CspCI CAANNNNNGTGG 2 cut(s) 111, 146
CviAII CATG 2 cut(s) 746, 978
CviQI GTAC 1 cut(s) 700
DdeI CTNAG 5 cut(s) 660, 789, 969, 983, 1044
DpnI GATC 5 cut(s) 135, 271, 335, 539, 680
DpnII GATC 5 cut(s) 133, 269, 333, 537, 678
DrdI GACNNNNNNGTC 1 cut(s) 323
DriI GACNNNNNGTC 1 cut(s) 964
DseDI GACNNNNNNGTC 1 cut(s) 323
EaeI YGGCCR 2 cut(s) 33, 399
EagI CGGCCG 2 cut(s) 33, 399
Eam1104I CTCTTC 7 cut(s) 69, 75, 150, 156, 162, 168, 186
Eam1105I GACNNNNNGTC 1 cut(s) 964
EarI CTCTTC 7 cut(s) 69, 75, 150, 156, 162, 168, 186
EciI GGCGGA 1 cut(s) 446
EclXI CGGCCG 2 cut(s) 33, 399
Eco130I CCWWGG 1 cut(s) 1074
Eco24I GRGCYC 1 cut(s) 444
Eco31I GGTCTC 2 cut(s) 275, 428
Eco47I GGWCC 1 cut(s) 724
Eco47III AGCGCT 1 cut(s) 358
Eco52I CGGCCG 2 cut(s) 33, 399
Eco57I CTGAAG 1 cut(s) 563
EcoO109I RGGNCCY 1 cut(s) 724
EcoRII CCWGG 1 cut(s) 702
EcoT14I CCWWGG 1 cut(s) 1074
EcoT38I GRGCYC 1 cut(s) 444
ErhI CCWWGG 1 cut(s) 1074
Esp3I CGTCTC 1 cut(s) 343
FaeI CATG 2 cut(s) 749, 981
FaiI YATR 4 cut(s) 365, 554, 747, 979
FatI CATG 2 cut(s) 745, 977
FauI CCCGC 2 cut(s) 36, 355
FblI GTMKAC 1 cut(s) 69
Fnu4HI GCNGC 7 cut(s) 36, 402, 478, 506, 582, 836, 1112
FokI GGATG 3 cut(s) 503, 728, 968
FriOI GRGCYC 1 cut(s) 444
Fsp4HI GCNGC 7 cut(s) 36, 402, 478, 506, 582, 836, 1112
FspBI CTAG 1 cut(s) 1032
GlaI GCGC 2 cut(s) 358, 405
GluI GCNGC 7 cut(s) 36, 402, 478, 506, 582, 836, 1112
GsaI CCCAGC 2 cut(s) 372, 447
GsuI CTGGAG 1 cut(s) 729
HaeII RGCGCY 1 cut(s) 360
HaeIII GGCC 4 cut(s) 35, 401, 794, 878
HapII CCGG 8 cut(s) 32, 267, 273, 414, 428, 728, 884, 953
HgaI GACGC 1 cut(s) 110
HhaI GCGC 2 cut(s) 359, 406
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 749, 981
Hin6I GCGC 2 cut(s) 357, 404
HinP1I GCGC 2 cut(s) 357, 404
HincII GTYRAC 4 cut(s) 70, 115, 669, 859
HindII GTYRAC 4 cut(s) 70, 115, 669, 859
HindIII AAGCTT 1 cut(s) 1142
HinfI GANTC 5 cut(s) 50, 263, 287, 801, 1181
HpaI GTTAAC 1 cut(s) 859
HpaII CCGG 8 cut(s) 32, 267, 273, 414, 428, 728, 884, 953
HphI GGTGA 1 cut(s) 313
Hpy166II GTNNAC 5 cut(s) 70, 115, 669, 859, 1013
Hpy188III TCNNGA 6 cut(s) 223, 267, 541, 619, 682, 1138
Hpy8I GTNNAC 5 cut(s) 70, 115, 669, 859, 1013
Hpy99I CGWCG 4 cut(s) 71, 74, 104, 485
HpyAV CCTTC 6 cut(s) 80, 463, 538, 811, 1065, 1192
HpyCH4III ACNGT 4 cut(s) 67, 325, 768, 871
HpyCH4IV ACGT 1 cut(s) 894
HpyCH4V TGCA 6 cut(s) 631, 771, 916, 1013, 1111, 1156
HpyF10VI GCNNNNNNNGC 2 cut(s) 356, 748
HpyF3I CTNAG 5 cut(s) 660, 789, 969, 983, 1044
HpySE526I ACGT 1 cut(s) 894
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 749, 981
HspAI GCGC 2 cut(s) 357, 404
Kpn2I TCCGGA 1 cut(s) 266
KroI GCCGGC 1 cut(s) 31
KroNI GCCGGC 1 cut(s) 33
KspAI GTTAAC 1 cut(s) 859
Kzo9I GATC 5 cut(s) 133, 269, 333, 537, 678
LmnI GCTCC 3 cut(s) 10, 516, 604
Lsp1109I GCAGC 2 cut(s) 517, 1123
LweI GCATC 3 cut(s) 481, 1041, 1143
MaeI CTAG 1 cut(s) 1032
MaeII ACGT 1 cut(s) 894
MaeIII GTNAC 2 cut(s) 220, 925
MalI GATC 5 cut(s) 135, 271, 335, 539, 680
MboI GATC 5 cut(s) 133, 269, 333, 537, 678
MfeI CAATTG 2 cut(s) 653, 1006
MflI RGATCY 2 cut(s) 133, 269
MhlI GDGCHC 3 cut(s) 444, 647, 1015
MluCI AATT 2 cut(s) 653, 1006
MlyI GAGTC 2 cut(s) 296, 795
MmeI TCCRAC 4 cut(s) 258, 339, 1002, 1068
MroI TCCGGA 1 cut(s) 266
MroNI GCCGGC 1 cut(s) 31
MseI TTAA 4 cut(s) 753, 858, 942, 1028
MslI CAYNNNNRTG 1 cut(s) 1121
MspCI CTTAAG 1 cut(s) 752
MspI CCGG 8 cut(s) 32, 267, 273, 414, 428, 728, 884, 953
MspR9I CCNGG 5 cut(s) 274, 415, 704, 728, 953
MunI CAATTG 2 cut(s) 653, 1006
MvaI CCWGG 1 cut(s) 704
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 2 cut(s) 356, 748
NaeI GCCGGC 1 cut(s) 33
NciI CCSGG 4 cut(s) 274, 415, 728, 953
NdeII GATC 5 cut(s) 133, 269, 333, 537, 678
NgoMIV GCCGGC 1 cut(s) 31
NlaIII CATG 2 cut(s) 749, 981
NlaIV GGNNCC 4 cut(s) 6, 271, 600, 726
NmeAIII GCCGAG 1 cut(s) 864
NmuCI GTSAC 2 cut(s) 220, 925
NspV TTCGAA 3 cut(s) 561, 590, 737
PcsI WCGNNNNNNNCGW 1 cut(s) 480
PdiI GCCGGC 1 cut(s) 33
PfeI GAWTC 3 cut(s) 50, 263, 1181
PflFI GACNNNGTC 1 cut(s) 286
PkrI GCNGC 7 cut(s) 37, 403, 479, 507, 583, 837, 1113
Ple19I CGATCG 1 cut(s) 336
PleI GAGTC 2 cut(s) 295, 795
PpsI GAGTC 2 cut(s) 295, 795
PpuMI RGGWCCY 1 cut(s) 724
Psp5II RGGWCCY 1 cut(s) 724
Psp6I CCWGG 1 cut(s) 702
PspFI CCCAGC 2 cut(s) 368, 443
PspGI CCWGG 1 cut(s) 702
PspN4I GGNNCC 4 cut(s) 6, 271, 600, 726
PspPI GGNCC 1 cut(s) 724
PspPPI RGGWCCY 1 cut(s) 724
PsuI RGATCY 2 cut(s) 133, 269
PsyI GACNNNGTC 1 cut(s) 286
PvuI CGATCG 1 cut(s) 336
RsaI GTAC 1 cut(s) 701
RsaNI GTAC 1 cut(s) 700
RseI CAYNNNNRTG 1 cut(s) 1121
SalI GTCGAC 1 cut(s) 68
SaqAI TTAA 4 cut(s) 753, 858, 942, 1028
SatI GCNGC 7 cut(s) 36, 402, 478, 506, 582, 836, 1112
Sau3AI GATC 5 cut(s) 133, 269, 333, 537, 678
Sau96I GGNCC 1 cut(s) 724
SchI GAGTC 2 cut(s) 296, 795
ScrFI CCNGG 5 cut(s) 274, 415, 704, 728, 953
SduI GDGCHC 3 cut(s) 444, 647, 1015
SfaNI GCATC 3 cut(s) 481, 1041, 1143
SfuI TTCGAA 3 cut(s) 561, 590, 737
SgrDI CGTCGACG 1 cut(s) 68
SinI GGWCC 1 cut(s) 724
SmiMI CAYNNNNRTG 1 cut(s) 1121
SmlI CTYRAG 2 cut(s) 608, 752
SmoI CTYRAG 2 cut(s) 608, 752
Sse9I AATT 2 cut(s) 653, 1006
SspMI CTAG 1 cut(s) 1032
StyD4I CCNGG 5 cut(s) 272, 413, 702, 726, 951
StyI CCWWGG 1 cut(s) 1074
TaaI ACNGT 4 cut(s) 67, 325, 768, 871
TaiI ACGT 1 cut(s) 897
TaqI TCGA 8 cut(s) 69, 419, 561, 590, 636, 737, 804, 1184
TaqII GACCGA 2 cut(s) 299, 452
TasI AATT 2 cut(s) 653, 1006
TauI GCSGC 5 cut(s) 38, 404, 480, 584, 838
TfiI GAWTC 3 cut(s) 50, 263, 1181
Tru1I TTAA 4 cut(s) 753, 858, 942, 1028
Tru9I TTAA 4 cut(s) 753, 858, 942, 1028
TseFI GTSAC 2 cut(s) 220, 925
TseI GCWGC 2 cut(s) 505, 1111
Tsp45I GTSAC 2 cut(s) 220, 925
TspDTI ATGAA 3 cut(s) 75, 604, 1202
Tth111I GACNNNGTC 1 cut(s) 286
Vha464I CTTAAG 1 cut(s) 752
VneI GTGCAC 1 cut(s) 1011
VpaK11BI GGWCC 1 cut(s) 724
XmiI GTMKAC 1 cut(s) 69
XspI CTAG 1 cut(s) 1032
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.