FvH4_6g46390

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005301
Physical Location & Seq
Reverse (-)
35400137 .. 35403104
2968 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g46390.t2

Sequence Viewer

Length: 663 bp
ATGGCTGAGGGAACAGCTGGAACCAAACCTGCGAAGAAGCTTGTTGAGATTGATATAGTTTCGGATACTGTATGTCCATGGTGCTTTGTGGGCAAGAAGAATCTTGACAAGGCTGTGGAGGCATCCAAAGGTCAATATGACTTTAGGATTAGATGGCACCCATATCAGCTTAATCCAAATGCCCCTAAAGAAGGTGTTGATAAGAGGAAGTATTATGAGCAAAAATTTGGGTCTGATATGTCTGCAAAAATGGCAAATAGGATGTCTGAGATTTTCAGAAGCCATGGCCTCGAATATGACCTGTCTGGACTGACGGGAAATACTCTAGATAGTCACAGGCTTATTTACTTTGCTGGGCAGCAGGATCCTGCGAAGCAACATGATCTTGTTGGTGAGCTCTTTCTTGGGTACTTCACACAGGCAAAGTACATTGGGGACAGGGAATATCTTCTGGAATGTGCTAGGAAAATTGGTGTAGAAGGAGCAGCAGAGTTTCTTGCAGACCCCAACAATGGGCTCAGTGAGGTTAGAGAAGACCTTAAGAAGTACTCAGGCAACATAAATGGAGTCCCACATTTCATGATTAATAATGGAAAGCAAGCATTGAGTGGTGCTCAACCGCCTGAGATTTTCTTGAAAGCTTTTGAAGTAGTAACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

221

Amino Acids

24.65

Weight (kDa)

6.96

Isoelectric Point (pI)

32.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 17 - 215 3.3e-34 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 156
AciI CCGC 1 cut(s) 620
AclWI GGATC 2 cut(s) 359, 372
AcsI RAATTY 1 cut(s) 224
AfaI GTAC 3 cut(s) 410, 428, 548
AfiI CCNNNNNNNGG 3 cut(s) 191, 512, 513
AflII CTTAAG 1 cut(s) 539
AgsI TTSAA 2 cut(s) 637, 647
AluBI AGCT 5 cut(s) 17, 40, 169, 397, 641
AluI AGCT 5 cut(s) 17, 40, 169, 397, 641
Alw21I GWGCWC 2 cut(s) 399, 616
AlwI GGATC 2 cut(s) 359, 372
AoxI GGCC 1 cut(s) 286
ApeKI GCWGC 2 cut(s) 358, 485
ApoI RAATTY 1 cut(s) 224
ArsI GACNNNNNNTTYG 2 cut(s) 248, 280
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 447
AsuHPI GGTGA 1 cut(s) 404
BamHI GGATCC 1 cut(s) 364
BanI GGYRCC 1 cut(s) 156
BanII GRGCYC 2 cut(s) 399, 519
BbsI GAAGAC 1 cut(s) 540
Bbv12I GWGCWC 2 cut(s) 399, 616
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 2 cut(s) 370, 497
BccI CCATC 1 cut(s) 147
BciVI GTATCC 1 cut(s) 58
BfaI CTAG 2 cut(s) 326, 462
BfrI CTTAAG 1 cut(s) 539
BfuAI ACCTGC 1 cut(s) 37
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 2 cut(s) 359, 486
BlsI GCNGC 2 cut(s) 360, 487
BmcAI AGTACT 1 cut(s) 548
BmiI GGNNCC 3 cut(s) 22, 158, 366
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 1 cut(s) 540
BplI GAGNNNNNCTC 2 cut(s) 598, 630
Bpu10I CCTNAGC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 77, 283
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 512, 513
BseDI CCNNGG 2 cut(s) 77, 283
BseGI GGATG 2 cut(s) 122, 267
BseLI CCNNNNNNNGG 3 cut(s) 191, 512, 513
BseMII CTCAG 4 cut(s) 258, 532, 564, 615
BseXI GCAGC 2 cut(s) 370, 497
BseYI CCCAGC 1 cut(s) 353
BshFI GGCC 1 cut(s) 288
BshNI GGYRCC 1 cut(s) 156
BsiHKAI GWGCWC 2 cut(s) 399, 616
BslFI GGGAC 2 cut(s) 449, 554
BslI CCNNNNNNNGG 3 cut(s) 191, 512, 513
BsmFI GGGAC 2 cut(s) 449, 554
BsnI GGCC 1 cut(s) 288
Bsp1286I GDGCHC 3 cut(s) 399, 519, 616
Bsp143I GATC 2 cut(s) 364, 382
Bsp19I CCATGG 2 cut(s) 77, 283
BspACI CCGC 1 cut(s) 620
BspANI GGCC 1 cut(s) 288
BspCNI CTCAG 4 cut(s) 259, 531, 563, 616
BspHI TCATGA 1 cut(s) 579
BspLI GGNNCC 3 cut(s) 22, 158, 366
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 2 cut(s) 359, 372
BspT107I GGYRCC 1 cut(s) 156
BspTI CTTAAG 1 cut(s) 539
BssECI CCNNGG 2 cut(s) 77, 283
BssMI GATC 2 cut(s) 364, 382
BssT1I CCWWGG 2 cut(s) 77, 283
Bst4CI ACNGT 1 cut(s) 70
BstAFI CTTAAG 1 cut(s) 539
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 5 cut(s) 6, 267, 518, 550, 624
BstDSI CCRYGG 2 cut(s) 77, 283
BstENI CCTNNNNNAGG 1 cut(s) 189
BstF5I GGATG 2 cut(s) 122, 267
BstKTI GATC 2 cut(s) 367, 385
BstMBI GATC 2 cut(s) 364, 382
BstMWI GCNNNNNNNGC 3 cut(s) 90, 119, 251
BstV1I GCAGC 2 cut(s) 370, 497
BstV2I GAAGAC 1 cut(s) 540
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 288
BtgI CCRYGG 2 cut(s) 77, 283
BtsCI GGATG 2 cut(s) 122, 267
BtsIMutI CAGTG 1 cut(s) 526
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 600
CciI TCATGA 1 cut(s) 579
Csp6I GTAC 3 cut(s) 409, 427, 547
CviAII CATG 4 cut(s) 78, 284, 380, 580
CviQI GTAC 3 cut(s) 409, 427, 547
DdeI CTNAG 5 cut(s) 6, 267, 518, 550, 624
DpnI GATC 2 cut(s) 366, 384
DpnII GATC 2 cut(s) 364, 382
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 77, 283
Eco24I GRGCYC 2 cut(s) 399, 519
Eco53kI GAGCTC 1 cut(s) 397
EcoICRI GAGCTC 1 cut(s) 397
EcoNI CCTNNNNNAGG 1 cut(s) 189
EcoT14I CCWWGG 2 cut(s) 77, 283
EcoT38I GRGCYC 2 cut(s) 399, 519
ErhI CCWWGG 2 cut(s) 77, 283
FaeI CATG 4 cut(s) 81, 287, 383, 583
FaqI GGGAC 2 cut(s) 449, 554
FatI CATG 4 cut(s) 77, 283, 379, 579
Fnu4HI GCNGC 2 cut(s) 359, 486
FokI GGATG 2 cut(s) 109, 274
FriOI GRGCYC 2 cut(s) 399, 519
Fsp4HI GCNGC 2 cut(s) 359, 486
FspBI CTAG 2 cut(s) 326, 462
GluI GCNGC 2 cut(s) 359, 486
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 1 cut(s) 288
Hin1II CATG 4 cut(s) 81, 287, 383, 583
HindIII AAGCTT 2 cut(s) 38, 639
HinfI GANTC 2 cut(s) 100, 567
HphI GGTGA 1 cut(s) 404
Hpy188I TCNGA 4 cut(s) 64, 235, 268, 278
Hpy188III TCNNGA 6 cut(s) 104, 306, 326, 452, 580, 634
HpyAV CCTTC 2 cut(s) 185, 473
HpyCH4III ACNGT 1 cut(s) 70
HpyCH4V TGCA 2 cut(s) 245, 500
HpyF10VI GCNNNNNNNGC 3 cut(s) 90, 119, 251
HpyF3I CTNAG 5 cut(s) 6, 267, 518, 550, 624
Hsp92II CATG 4 cut(s) 81, 287, 383, 583
Kzo9I GATC 2 cut(s) 364, 382
LmnI GCTCC 1 cut(s) 482
Lsp1109I GCAGC 2 cut(s) 370, 497
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 326, 462
MaeIII GTNAC 2 cut(s) 332, 652
MalI GATC 2 cut(s) 366, 384
MboI GATC 2 cut(s) 364, 382
MboII GAAGA 4 cut(s) 46, 109, 440, 545
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 3 cut(s) 399, 519, 616
MluCI AATT 2 cut(s) 224, 468
MlyI GAGTC 1 cut(s) 576
MnlI CCTC 4 cut(s) 112, 198, 299, 517
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 3 cut(s) 171, 540, 585
MspA1I CMGCKG 1 cut(s) 17
MspCI CTTAAG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 90, 119, 251
NcoI CCATGG 2 cut(s) 77, 283
NdeII GATC 2 cut(s) 364, 382
NlaIII CATG 4 cut(s) 81, 287, 383, 583
NlaIV GGNNCC 3 cut(s) 22, 158, 366
NmuCI GTSAC 1 cut(s) 332
PagI TCATGA 1 cut(s) 579
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 2 cut(s) 360, 487
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
PshBI ATTAAT 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 399
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 3 cut(s) 22, 158, 366
PsuI RGATCY 1 cut(s) 364
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 3 cut(s) 410, 428, 548
RsaNI GTAC 3 cut(s) 409, 427, 547
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 3 cut(s) 171, 540, 585
SatI GCNGC 2 cut(s) 359, 486
Sau3AI GATC 2 cut(s) 364, 382
ScaI AGTACT 1 cut(s) 548
SchI GAGTC 1 cut(s) 576
SduI GDGCHC 3 cut(s) 399, 519, 616
SfaNI GCATC 1 cut(s) 131
SmlI CTYRAG 1 cut(s) 539
SmoI CTYRAG 1 cut(s) 539
Sse9I AATT 2 cut(s) 224, 468
SsiI CCGC 1 cut(s) 620
SspMI CTAG 2 cut(s) 326, 462
SstI GAGCTC 1 cut(s) 399
StyI CCWWGG 2 cut(s) 77, 283
TaaI ACNGT 1 cut(s) 70
TaqI TCGA 1 cut(s) 291
TasI AATT 2 cut(s) 224, 468
TatI WGTACW 2 cut(s) 426, 546
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 3 cut(s) 171, 540, 585
Tru9I TTAA 3 cut(s) 171, 540, 585
TscAI CASTG 1 cut(s) 526
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 2 cut(s) 358, 485
Tsp45I GTSAC 1 cut(s) 332
TspDTI ATGAA 1 cut(s) 568
TspRI CASTG 1 cut(s) 526
Vha464I CTTAAG 1 cut(s) 539
VspI ATTAAT 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 189
XapI RAATTY 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 326, 462
ZrmI AGTACT 1 cut(s) 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.