MD17G1065700.v1.1

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0005301
Physical Location & Seq
Forward (+)
5312558 .. 5315068
2511 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1065700.v1.1.491

Sequence Viewer

Length: 705 bp
ATGAAGGCATCAAATCGTTCTAATTTATTAGATTCATATGTTTCAAATAGAAACATGGCTGGTGTGTCGGCTGGGAAGAAGCTCATTCAAATTGACATCGTGTCGGATACGGTGTGCCCGTGGTGCTTTGTGGGCAAGAGAAATCTTGAAAAAGCTTTTGAGGCATCTCGTGATCGATTCGACTTTGAGGTTAGATGGCATCCATTCCAACTTAATCCCAATGCGCCTAAAGAAGGCGTTGATAAGAGAAGTTATTATGAGGAAAAATTTGGGACTAATAGGTCGAAACAAATGGAGGCTCGGATGGCAGAGATTTTCCGAAGCCACGGCCTGGAATATAACCTGTCCGGGCTCACTGGAAGTACTCTAGATAGCCACAGGCTTATCTATTTTGCTGGGCTTCAAGGGCATGATAAGCAACACGCTCTTGTGGCTGAACTATTTCTTGGATACTTCACACAGGCAAAATACATCGGGGACAGGGAATTTCTTCTGGAAAGTGCTCAAAAGGTTGGAGTAGAAGGGGCAGCAGAGTTTCTTGAAGACCCCAACAATGGGCTCAATGAGGTCAATGAAGAGCTCGAGAAGTACACAAGAAACATCAATGGAGTCCCACATTTTTTGTTTAACAATGGAAAGCTTGCGTTAAGTGGCGGTCAACCGCCCGAGGTGTTCTTGAGAGCTTTTGAAGCAGCCACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

235

Amino Acids

26.45

Weight (kDa)

6.33

Isoelectric Point (pI)

34.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 30 - 229 1e-33 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 280
AccB7I CCANNNNNTGG 1 cut(s) 331
AciI CCGC 2 cut(s) 654, 662
AcsI RAATTY 2 cut(s) 266, 485
AdeI CACNNNGTG 1 cut(s) 702
AfaI GTAC 2 cut(s) 364, 590
AfiI CCNNNNNNNGG 4 cut(s) 233, 331, 554, 555
AgsI TTSAA 6 cut(s) 45, 89, 149, 404, 542, 689
AhdI GACNNNNNGTC 1 cut(s) 100
AjnI CCWGG 1 cut(s) 330
AjuI GAANNNNNNNTTGG 2 cut(s) 429, 461
AluBI AGCT 5 cut(s) 82, 155, 580, 640, 683
AluI AGCT 5 cut(s) 82, 155, 580, 640, 683
Alw21I GWGCWC 2 cut(s) 505, 582
Ama87I CYCGRG 2 cut(s) 581, 665
AoxI GGCC 1 cut(s) 328
ApeKI GCWGC 2 cut(s) 527, 692
ApoI RAATTY 2 cut(s) 266, 485
Asp700I GAANNNNTTC 2 cut(s) 441, 489
AspLEI GCGC 1 cut(s) 226
AsuC2I CCSGG 1 cut(s) 349
AvaI CYCGRG 2 cut(s) 581, 665
BaeGI GKGCMC 1 cut(s) 119
BanII GRGCYC 3 cut(s) 354, 561, 582
BauI CACGAG 1 cut(s) 168
BbsI GAAGAC 1 cut(s) 549
Bbv12I GWGCWC 2 cut(s) 505, 582
BbvI GCAGC 1 cut(s) 539
BccI CCATC 2 cut(s) 189, 298
BceAI ACGGC 1 cut(s) 343
BciT130I CCWGG 1 cut(s) 332
BciVI GTATCC 2 cut(s) 100, 443
BcnI CCSGG 1 cut(s) 349
BfaI CTAG 1 cut(s) 368
BfuI GTATCC 2 cut(s) 100, 443
BisI GCNGC 2 cut(s) 528, 693
BlsI GCNGC 2 cut(s) 529, 694
BmcAI AGTACT 1 cut(s) 364
Bme1390I CCNGG 2 cut(s) 332, 349
BmeRI GACNNNNNGTC 1 cut(s) 100
BmeT110I CYCGRG 2 cut(s) 581, 665
BmrFI CCNGG 2 cut(s) 332, 349
BmsI GCATC 3 cut(s) 17, 173, 208
BpiI GAAGAC 1 cut(s) 549
BpuEI CTTGAG 1 cut(s) 697
BpuMI CCSGG 1 cut(s) 349
Bsa29I ATCGAT 1 cut(s) 175
BsaJI CCNNGG 3 cut(s) 119, 325, 666
Bsc4I CCNNNNNNNGG 4 cut(s) 233, 331, 554, 555
Bse1I ACTGG 1 cut(s) 361
BseBI CCWGG 1 cut(s) 332
BseCI ATCGAT 1 cut(s) 175
BseDI CCNNGG 3 cut(s) 119, 325, 666
BseGI GGATG 2 cut(s) 199, 309
BseLI CCNNNNNNNGG 4 cut(s) 233, 331, 554, 555
BseNI ACTGG 1 cut(s) 361
BseSI GKGCMC 1 cut(s) 119
BseXI GCAGC 1 cut(s) 539
BseYI CCCAGC 2 cut(s) 71, 395
BshFI GGCC 1 cut(s) 330
BshVI ATCGAT 1 cut(s) 175
BsiHKAI GWGCWC 2 cut(s) 505, 582
BsiHKCI CYCGRG 2 cut(s) 581, 665
BsiSI CCGG 1 cut(s) 348
BslFI GGGAC 3 cut(s) 286, 491, 596
BslI CCNNNNNNNGG 4 cut(s) 233, 331, 554, 555
BsmFI GGGAC 3 cut(s) 286, 491, 596
BsnI GGCC 1 cut(s) 330
BsoBI CYCGRG 2 cut(s) 581, 665
Bsp1286I GDGCHC 5 cut(s) 119, 354, 505, 561, 582
Bsp143I GATC 1 cut(s) 172
BspACI CCGC 2 cut(s) 654, 662
BspANI GGCC 1 cut(s) 330
BspDI ATCGAT 1 cut(s) 175
BspQI GCTCTTC 1 cut(s) 570
BsrI ACTGG 1 cut(s) 361
BssECI CCNNGG 3 cut(s) 119, 325, 666
BssMI GATC 1 cut(s) 172
BssSI CACGAG 1 cut(s) 168
Bst2BI CACGAG 1 cut(s) 168
Bst2UI CCWGG 1 cut(s) 332
Bst4CI ACNGT 1 cut(s) 112
Bst6I CTCTTC 1 cut(s) 570
BstC8I GCNNGC 1 cut(s) 642
BstDSI CCRYGG 2 cut(s) 119, 325
BstENI CCTNNNNNAGG 1 cut(s) 231
BstF5I GGATG 2 cut(s) 199, 309
BstHHI GCGC 1 cut(s) 226
BstKTI GATC 1 cut(s) 175
BstMBI GATC 1 cut(s) 172
BstMWI GCNNNNNNNGC 8 cut(s) 123, 132, 161, 305, 406, 415, 431, 689
BstNI CCWGG 1 cut(s) 332
BstSCI CCNGG 2 cut(s) 330, 347
BstSLI GKGCMC 1 cut(s) 119
BstV1I GCAGC 1 cut(s) 539
BstV2I GAAGAC 1 cut(s) 549
Bsu15I ATCGAT 1 cut(s) 175
BsuI GTATCC 2 cut(s) 100, 443
BsuRI GGCC 1 cut(s) 330
BsuTUI ATCGAT 1 cut(s) 175
BtgI CCRYGG 2 cut(s) 119, 325
BtsCI GGATG 2 cut(s) 199, 309
BtsIMutI CAGTG 1 cut(s) 354
Cac8I GCNNGC 1 cut(s) 642
CfoI GCGC 1 cut(s) 226
ClaI ATCGAT 1 cut(s) 175
Csp6I GTAC 2 cut(s) 363, 589
CspCI CAANNNNNGTGG 2 cut(s) 603, 638
CviAII CATG 2 cut(s) 55, 410
CviQI GTAC 2 cut(s) 363, 589
DpnI GATC 1 cut(s) 174
DpnII GATC 1 cut(s) 172
DraIII CACNNNGTG 1 cut(s) 702
DrdI GACNNNNNNGTC 1 cut(s) 280
DriI GACNNNNNGTC 1 cut(s) 100
DseDI GACNNNNNNGTC 1 cut(s) 280
Eam1104I CTCTTC 1 cut(s) 570
Eam1105I GACNNNNNGTC 1 cut(s) 100
EarI CTCTTC 1 cut(s) 570
Ecl136II GAGCTC 1 cut(s) 580
Eco24I GRGCYC 3 cut(s) 354, 561, 582
Eco53kI GAGCTC 1 cut(s) 580
Eco88I CYCGRG 2 cut(s) 581, 665
EcoICRI GAGCTC 1 cut(s) 580
EcoNI CCTNNNNNAGG 1 cut(s) 231
EcoRII CCWGG 1 cut(s) 330
EcoT38I GRGCYC 3 cut(s) 354, 561, 582
FaeI CATG 2 cut(s) 58, 413
FaiI YATR 6 cut(s) 37, 39, 56, 258, 339, 411
FaqI GGGAC 3 cut(s) 286, 491, 596
FatI CATG 2 cut(s) 54, 409
FauNDI CATATG 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 528, 693
FokI GGATG 2 cut(s) 186, 316
FriOI GRGCYC 3 cut(s) 354, 561, 582
Fsp4HI GCNGC 2 cut(s) 528, 693
FspBI CTAG 1 cut(s) 368
GlaI GCGC 1 cut(s) 225
GluI GCNGC 2 cut(s) 528, 693
GsaI CCCAGC 2 cut(s) 75, 399
HaeIII GGCC 1 cut(s) 330
HapII CCGG 1 cut(s) 348
HhaI GCGC 1 cut(s) 226
Hin1II CATG 2 cut(s) 58, 413
Hin6I GCGC 1 cut(s) 224
HinP1I GCGC 1 cut(s) 224
HincII GTYRAC 1 cut(s) 659
HindII GTYRAC 1 cut(s) 659
HindIII AAGCTT 2 cut(s) 153, 638
HinfI GANTC 3 cut(s) 32, 177, 609
HpaII CCGG 1 cut(s) 348
Hpy166II GTNNAC 2 cut(s) 591, 659
Hpy188I TCNGA 3 cut(s) 106, 303, 320
Hpy188III TCNNGA 7 cut(s) 146, 170, 368, 494, 539, 583, 676
Hpy8I GTNNAC 2 cut(s) 591, 659
HpyAV CCTTC 2 cut(s) 227, 515
HpyCH4III ACNGT 1 cut(s) 112
HpyF10VI GCNNNNNNNGC 8 cut(s) 123, 132, 161, 305, 406, 415, 431, 689
Hsp92II CATG 2 cut(s) 58, 413
HspAI GCGC 1 cut(s) 224
Kzo9I GATC 1 cut(s) 172
LguI GCTCTTC 1 cut(s) 570
Lsp1109I GCAGC 1 cut(s) 539
LweI GCATC 3 cut(s) 17, 173, 208
MaeI CTAG 1 cut(s) 368
MalI GATC 1 cut(s) 174
MboI GATC 1 cut(s) 172
MboII GAAGA 4 cut(s) 88, 482, 554, 587
MhlI GDGCHC 5 cut(s) 119, 354, 505, 561, 582
MluCI AATT 4 cut(s) 22, 90, 266, 485
MlyI GAGTC 1 cut(s) 618
MmeI TCCRAC 3 cut(s) 84, 232, 493
MnlI CCTC 6 cut(s) 154, 181, 253, 289, 559, 661
MroXI GAANNNNTTC 2 cut(s) 441, 489
MseI TTAA 3 cut(s) 213, 627, 647
MspI CCGG 1 cut(s) 348
MspR9I CCNGG 2 cut(s) 332, 349
MvaI CCWGG 1 cut(s) 332
MwoI GCNNNNNNNGC 8 cut(s) 123, 132, 161, 305, 406, 415, 431, 689
NciI CCSGG 1 cut(s) 349
NdeI CATATG 1 cut(s) 37
NdeII GATC 1 cut(s) 172
NlaIII CATG 2 cut(s) 58, 413
PaeR7I CTCGAG 1 cut(s) 581
PciSI GCTCTTC 1 cut(s) 570
PcsI WCGNNNNNNNCGW 1 cut(s) 116
PdmI GAANNNNTTC 2 cut(s) 441, 489
PfeI GAWTC 2 cut(s) 32, 177
PflMI CCANNNNNTGG 1 cut(s) 331
PkrI GCNGC 2 cut(s) 529, 694
PleI GAGTC 1 cut(s) 617
PpsI GAGTC 1 cut(s) 617
Psp124BI GAGCTC 1 cut(s) 582
Psp6I CCWGG 1 cut(s) 330
PspFI CCCAGC 2 cut(s) 71, 395
PspGI CCWGG 1 cut(s) 330
RsaI GTAC 2 cut(s) 364, 590
RsaNI GTAC 2 cut(s) 363, 589
SacI GAGCTC 1 cut(s) 582
SapI GCTCTTC 1 cut(s) 570
SaqAI TTAA 3 cut(s) 213, 627, 647
SatI GCNGC 2 cut(s) 528, 693
Sau3AI GATC 1 cut(s) 172
ScaI AGTACT 1 cut(s) 364
SchI GAGTC 1 cut(s) 618
ScrFI CCNGG 2 cut(s) 332, 349
SduI GDGCHC 5 cut(s) 119, 354, 505, 561, 582
SfaNI GCATC 3 cut(s) 17, 173, 208
Sfr274I CTCGAG 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 581
SmlI CTYRAG 2 cut(s) 581, 676
SmoI CTYRAG 2 cut(s) 581, 676
Sse9I AATT 4 cut(s) 22, 90, 266, 485
SsiI CCGC 2 cut(s) 654, 662
SspMI CTAG 1 cut(s) 368
SstI GAGCTC 1 cut(s) 582
StyD4I CCNGG 2 cut(s) 330, 347
TaaI ACNGT 1 cut(s) 112
TaqI TCGA 4 cut(s) 175, 180, 284, 582
TasI AATT 4 cut(s) 22, 90, 266, 485
TatI WGTACW 2 cut(s) 362, 588
TfiI GAWTC 2 cut(s) 32, 177
Tru1I TTAA 3 cut(s) 213, 627, 647
Tru9I TTAA 3 cut(s) 213, 627, 647
TscAI CASTG 1 cut(s) 361
TseI GCWGC 2 cut(s) 527, 692
TspDTI ATGAA 3 cut(s) 17, 24, 588
TspRI CASTG 1 cut(s) 361
Van91I CCANNNNNTGG 1 cut(s) 331
XagI CCTNNNNNAGG 1 cut(s) 231
XapI RAATTY 2 cut(s) 266, 485
XbaI TCTAGA 1 cut(s) 367
XhoI CTCGAG 1 cut(s) 581
XmnI GAANNNNTTC 2 cut(s) 441, 489
XspI CTAG 1 cut(s) 368
ZrmI AGTACT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.