MD09G1074300.v1.1

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0005301
Physical Location & Seq
Forward (+)
5162693 .. 5164884
2192 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1074300.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGCTGGTGTGAAAAAGCTCATCCAAATCGACATCGTTTCGGATACGGTATGCCCGTGGTGCTTTGTGGGCAAGAGAAATCTTGAAAAAGCTTTCGAGGCATCTCGTGATCGATTCAACTTTGAGGTTAGATGGCATCCATTCCAACTTAATCCCAATGCGCCTAAAGAAGGCGTTGATAAGAGAAGTTATTACGAGGAAAAATTCGGGTCTAGTAGGTCGAAACAAATGGAGACTCGGATGCAAGAGATTTTCAGAAGCCATGGCCTCGAATATAACCTGTCCGGACTCACGGGAAGTACTCTAGATAGCCACAGGCTTATCTATTTTGCTGGGACTCAAGGGCATGATAAGCAACACGATCTTGTGGGTGAACTGTTTCTAGGATACTTCACACAGGCAAAATACATCGGGGACAGGGAATATCTTCTGCAAAGTGCTCGAAAGGTCGGAGTAGAAGGGGCCGCAGAGTTTCTTGAAGACCCCAACAATGGGCTCGATGAGGTCAATGAAGAACTAAAGAAGTACTCGAGAAACATCAATGGAGTCCCACATTTTTTGATTAACAACGGAAAGCAAGCGTTAAGTGGCGGCCAGCCGCCTGAGGTGTTCTTGAGAGCTTTTGAAGCAGCTACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

214

Amino Acids

24.26

Weight (kDa)

6.61

Isoelectric Point (pI)

38.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 9 - 208 3.6e-35 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 284
AciI CCGC 3 cut(s) 465, 591, 599
AcoI YGGCCR 1 cut(s) 592
AcsI RAATTY 1 cut(s) 203
AfaI GTAC 2 cut(s) 301, 527
AfiI CCNNNNNNNGG 3 cut(s) 170, 491, 492
AgsI TTSAA 4 cut(s) 86, 118, 479, 626
AluBI AGCT 4 cut(s) 19, 92, 620, 632
AluI AGCT 4 cut(s) 19, 92, 620, 632
Alw21I GWGCWC 1 cut(s) 442
Alw26I GTCTC 1 cut(s) 227
Ama87I CYCGRG 1 cut(s) 529
Aor13HI TCCGGA 1 cut(s) 284
AoxI GGCC 3 cut(s) 265, 462, 592
ApeKI GCWGC 1 cut(s) 629
ApoI RAATTY 1 cut(s) 203
Asp700I GAANNNNTTC 2 cut(s) 378, 426
AspLEI GCGC 1 cut(s) 163
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 383
AvaI CYCGRG 1 cut(s) 529
AxyI CCTNAGG 1 cut(s) 603
BanII GRGCYC 1 cut(s) 498
BauI CACGAG 1 cut(s) 105
BbsI GAAGAC 1 cut(s) 486
Bbv12I GWGCWC 1 cut(s) 442
BccI CCATC 1 cut(s) 126
BcgI CGANNNNNNTGC 2 cut(s) 422, 456
BciVI GTATCC 2 cut(s) 37, 380
BcoDI GTCTC 1 cut(s) 227
BfaI CTAG 3 cut(s) 213, 305, 383
BfuI GTATCC 2 cut(s) 37, 380
BisI GCNGC 4 cut(s) 465, 592, 599, 630
BlsI GCNGC 4 cut(s) 466, 593, 600, 631
BmcAI AGTACT 2 cut(s) 301, 527
BmeT110I CYCGRG 1 cut(s) 529
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 463
BmsI GCATC 3 cut(s) 110, 145, 231
BpiI GAAGAC 1 cut(s) 486
BpuEI CTTGAG 2 cut(s) 324, 634
Bsa29I ATCGAT 1 cut(s) 112
BsaJI CCNNGG 2 cut(s) 56, 262
BsaWI WCCGGW 1 cut(s) 284
Bsc4I CCNNNNNNNGG 3 cut(s) 170, 491, 492
Bse21I CCTNAGG 1 cut(s) 603
BseAI TCCGGA 1 cut(s) 284
BseCI ATCGAT 1 cut(s) 112
BseDI CCNNGG 2 cut(s) 56, 262
BseGI GGATG 3 cut(s) 21, 136, 246
BseLI CCNNNNNNNGG 3 cut(s) 170, 491, 492
BseMII CTCAG 1 cut(s) 594
BseYI CCCAGC 1 cut(s) 332
BshFI GGCC 3 cut(s) 267, 464, 594
BshVI ATCGAT 1 cut(s) 112
BsiHKAI GWGCWC 1 cut(s) 442
BsiHKCI CYCGRG 1 cut(s) 529
BsiSI CCGG 1 cut(s) 285
BslFI GGGAC 3 cut(s) 349, 428, 533
BslI CCNNNNNNNGG 3 cut(s) 170, 491, 492
BsmAI GTCTC 1 cut(s) 227
BsmFI GGGAC 3 cut(s) 349, 428, 533
BsnI GGCC 3 cut(s) 267, 464, 594
BsoBI CYCGRG 1 cut(s) 529
Bsp1286I GDGCHC 2 cut(s) 442, 498
Bsp13I TCCGGA 1 cut(s) 284
Bsp143I GATC 2 cut(s) 109, 361
Bsp19I CCATGG 1 cut(s) 262
BspACI CCGC 3 cut(s) 465, 591, 599
BspANI GGCC 3 cut(s) 267, 464, 594
BspCNI CTCAG 1 cut(s) 595
BspDI ATCGAT 1 cut(s) 112
BspEI TCCGGA 1 cut(s) 284
BspLI GGNNCC 1 cut(s) 463
BssECI CCNNGG 2 cut(s) 56, 262
BssMI GATC 2 cut(s) 109, 361
BssSI CACGAG 1 cut(s) 105
BssT1I CCWWGG 1 cut(s) 262
Bst2BI CACGAG 1 cut(s) 105
Bst4CI ACNGT 2 cut(s) 49, 378
BstC8I GCNNGC 2 cut(s) 579, 596
BstDEI CTNAG 1 cut(s) 603
BstDSI CCRYGG 2 cut(s) 56, 262
BstENI CCTNNNNNAGG 1 cut(s) 168
BstF5I GGATG 3 cut(s) 21, 136, 246
BstHHI GCGC 1 cut(s) 163
BstKTI GATC 2 cut(s) 112, 364
BstMAI GTCTC 1 cut(s) 227
BstMBI GATC 2 cut(s) 109, 361
BstMWI GCNNNNNNNGC 5 cut(s) 60, 69, 98, 352, 626
BstV2I GAAGAC 1 cut(s) 486
Bsu15I ATCGAT 1 cut(s) 112
Bsu36I CCTNAGG 1 cut(s) 603
BsuI GTATCC 2 cut(s) 37, 380
BsuRI GGCC 3 cut(s) 267, 464, 594
BsuTUI ATCGAT 1 cut(s) 112
BtgI CCRYGG 2 cut(s) 56, 262
BtsCI GGATG 3 cut(s) 21, 136, 246
Cac8I GCNNGC 2 cut(s) 579, 596
CfoI GCGC 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 462
ClaI ATCGAT 1 cut(s) 112
Csp6I GTAC 2 cut(s) 300, 526
CspCI CAANNNNNGTGG 2 cut(s) 540, 575
CviAII CATG 2 cut(s) 263, 347
CviQI GTAC 2 cut(s) 300, 526
DdeI CTNAG 1 cut(s) 603
DpnI GATC 2 cut(s) 111, 363
DpnII GATC 2 cut(s) 109, 361
EaeI YGGCCR 1 cut(s) 592
Eco130I CCWWGG 1 cut(s) 262
Eco24I GRGCYC 1 cut(s) 498
Eco81I CCTNAGG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 529
EcoNI CCTNNNNNAGG 1 cut(s) 168
EcoT14I CCWWGG 1 cut(s) 262
EcoT38I GRGCYC 1 cut(s) 498
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 2 cut(s) 266, 350
FaiI YATR 4 cut(s) 52, 264, 276, 348
FaqI GGGAC 3 cut(s) 349, 428, 533
FatI CATG 2 cut(s) 262, 346
Fnu4HI GCNGC 4 cut(s) 465, 592, 599, 630
FokI GGATG 3 cut(s) 8, 123, 253
FriOI GRGCYC 1 cut(s) 498
Fsp4HI GCNGC 4 cut(s) 465, 592, 599, 630
FspBI CTAG 3 cut(s) 213, 305, 383
GlaI GCGC 1 cut(s) 162
GluI GCNGC 4 cut(s) 465, 592, 599, 630
GsaI CCCAGC 1 cut(s) 336
HaeIII GGCC 3 cut(s) 267, 464, 594
HapII CCGG 1 cut(s) 285
HhaI GCGC 1 cut(s) 163
Hin1II CATG 2 cut(s) 266, 350
Hin6I GCGC 1 cut(s) 161
HinP1I GCGC 1 cut(s) 161
HindIII AAGCTT 1 cut(s) 90
HinfI GANTC 5 cut(s) 114, 235, 288, 337, 546
HpaII CCGG 1 cut(s) 285
HphI GGTGA 1 cut(s) 383
Hpy166II GTNNAC 1 cut(s) 374
Hpy188I TCNGA 4 cut(s) 43, 240, 257, 452
Hpy188III TCNNGA 7 cut(s) 83, 107, 285, 305, 476, 531, 613
Hpy8I GTNNAC 1 cut(s) 374
HpyAV CCTTC 2 cut(s) 164, 452
HpyCH4III ACNGT 2 cut(s) 49, 378
HpyCH4V TGCA 2 cut(s) 244, 433
HpyF10VI GCNNNNNNNGC 5 cut(s) 60, 69, 98, 352, 626
HpyF3I CTNAG 1 cut(s) 603
Hsp92II CATG 2 cut(s) 266, 350
HspAI GCGC 1 cut(s) 161
Kpn2I TCCGGA 1 cut(s) 284
Kzo9I GATC 2 cut(s) 109, 361
LpnPI CCDG 8 cut(s) 293, 298, 301, 318, 383, 403, 608, 615
LweI GCATC 3 cut(s) 110, 145, 231
MaeI CTAG 3 cut(s) 213, 305, 383
MalI GATC 2 cut(s) 111, 363
MboI GATC 2 cut(s) 109, 361
MboII GAAGA 3 cut(s) 419, 491, 524
MhlI GDGCHC 2 cut(s) 442, 498
MluCI AATT 1 cut(s) 203
MlyI GAGTC 4 cut(s) 229, 282, 331, 555
MmeI TCCRAC 2 cut(s) 169, 430
MnlI CCTC 6 cut(s) 91, 118, 190, 278, 496, 598
MroI TCCGGA 1 cut(s) 284
MroXI GAANNNNTTC 2 cut(s) 378, 426
MseI TTAA 3 cut(s) 150, 564, 584
MspI CCGG 1 cut(s) 285
MwoI GCNNNNNNNGC 5 cut(s) 60, 69, 98, 352, 626
NcoI CCATGG 1 cut(s) 262
NdeII GATC 2 cut(s) 109, 361
NlaIII CATG 2 cut(s) 266, 350
NlaIV GGNNCC 1 cut(s) 463
PaeR7I CTCGAG 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PdmI GAANNNNTTC 2 cut(s) 378, 426
PfeI GAWTC 1 cut(s) 114
PkrI GCNGC 4 cut(s) 466, 593, 600, 631
PleI GAGTC 4 cut(s) 229, 282, 331, 554
PpsI GAGTC 4 cut(s) 229, 282, 331, 554
PspFI CCCAGC 1 cut(s) 332
PspN4I GGNNCC 1 cut(s) 463
PspPI GGNCC 1 cut(s) 462
RsaI GTAC 2 cut(s) 301, 527
RsaNI GTAC 2 cut(s) 300, 526
SaqAI TTAA 3 cut(s) 150, 564, 584
SatI GCNGC 4 cut(s) 465, 592, 599, 630
Sau3AI GATC 2 cut(s) 109, 361
Sau96I GGNCC 1 cut(s) 462
ScaI AGTACT 2 cut(s) 301, 527
SchI GAGTC 4 cut(s) 229, 282, 331, 555
SduI GDGCHC 2 cut(s) 442, 498
SfaNI GCATC 3 cut(s) 110, 145, 231
Sfr274I CTCGAG 1 cut(s) 529
SlaI CTCGAG 1 cut(s) 529
SmlI CTYRAG 3 cut(s) 339, 529, 613
SmoI CTYRAG 3 cut(s) 339, 529, 613
Sse9I AATT 1 cut(s) 203
SsiI CCGC 3 cut(s) 465, 591, 599
SspMI CTAG 3 cut(s) 213, 305, 383
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 2 cut(s) 49, 378
TaqI TCGA 8 cut(s) 30, 96, 112, 221, 270, 442, 498, 530
TasI AATT 1 cut(s) 203
TatI WGTACW 2 cut(s) 299, 525
TauI GCSGC 3 cut(s) 467, 594, 601
TfiI GAWTC 1 cut(s) 114
Tru1I TTAA 3 cut(s) 150, 564, 584
Tru9I TTAA 3 cut(s) 150, 564, 584
TseI GCWGC 1 cut(s) 629
TspDTI ATGAA 1 cut(s) 525
TspGWI ACGGA 1 cut(s) 585
XagI CCTNNNNNAGG 1 cut(s) 168
XapI RAATTY 1 cut(s) 203
XbaI TCTAGA 1 cut(s) 304
XhoI CTCGAG 1 cut(s) 529
XmnI GAANNNNTTC 2 cut(s) 378, 426
XspI CTAG 3 cut(s) 213, 305, 383
ZrmI AGTACT 2 cut(s) 301, 527
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.