Rroxscaffold_2G00086210

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0005301
Physical Location & Seq
Forward (+)
8451577 .. 8453547
1971 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00086210.1

Sequence Viewer

Length: 663 bp
ATGGCTGAGGGATCATCTGGAGCCAAACCTGCGAAGAAGCTCGTCGAAATTGATATAATTTCGGATACTGTGTGCCCATGGTGCTTTGTGGGCAAGAGGAATCTCGACAAGGCTGTGGAGGCATCCAACGGTCGATATGACTTTAGGATTAGATGGCACCCGTATCAGCTTAATCCTAATGCCCCTAAAGAAGGTCTTGATAAGAGAAAGTACTATGAGCAAAAATTTGGGTCTGATATGTCTGCAAAAATGGGCAACAGGATGTCAGAGATTTTCAGAAGCCATGGCCTCGAATATGACCTGTCTGGACTCACGGGAAATACTCTAGATAGTCACAGGCTTATCTACTTTGCCGCGCAGCAGGATCTTGCGAAGCAACATGATCTTGTTGGTGAGCTCTTTCTCGGGTACTTCACACAGGCAAAGTACATTGGGGACAGGGAATATCTTCTGGAATGTGCTAGGAAGGTTGGTGTTGAAGGAGCAGCAGAGTTTCTTGAAGACCCCAACAATGGCCTAAATGAGGTTAAAGAAGACCTTAATAAGTACTCGGGCAGCATAAACGGAGTCCCACATTTTGTGATTAATAATGGAAAGCAAGCATTGAGTGGTGCTCAACCGCCTGAGATTTTCTTGAAAGCTTTTGAAGTAGTAACAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

220

Amino Acids

24.67

Weight (kDa)

6.23

Isoelectric Point (pI)

34.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 17 - 215 1.2e-33 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 37
AccB1I GGYRCC 1 cut(s) 156
AccII CGCG 1 cut(s) 356
AciI CCGC 2 cut(s) 354, 620
AclWI GGATC 2 cut(s) 19, 372
AcsI RAATTY 1 cut(s) 224
AfaI GTAC 4 cut(s) 212, 410, 428, 548
AfiI CCNNNNNNNGG 3 cut(s) 191, 512, 523
AgsI TTSAA 4 cut(s) 479, 500, 637, 647
AluBI AGCT 4 cut(s) 40, 169, 397, 641
AluI AGCT 4 cut(s) 40, 169, 397, 641
Alw21I GWGCWC 2 cut(s) 399, 616
AlwI GGATC 2 cut(s) 19, 372
Ama87I CYCGRG 2 cut(s) 404, 550
AoxI GGCC 2 cut(s) 286, 514
ApeKI GCWGC 3 cut(s) 358, 485, 555
ApoI RAATTY 1 cut(s) 224
AseI ATTAAT 1 cut(s) 585
Asp700I GAANNNNTTC 1 cut(s) 447
AspLEI GCGC 1 cut(s) 358
AsuHPI GGTGA 1 cut(s) 404
AvaI CYCGRG 2 cut(s) 404, 550
BaeGI GKGCMC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 156
BanII GRGCYC 1 cut(s) 399
BbsI GAAGAC 2 cut(s) 507, 540
Bbv12I GWGCWC 2 cut(s) 399, 616
BbvCI CCTCAGC 1 cut(s) 6
BbvI GCAGC 3 cut(s) 370, 497, 567
BccI CCATC 1 cut(s) 147
BciVI GTATCC 1 cut(s) 58
BfaI CTAG 2 cut(s) 326, 462
BfuAI ACCTGC 1 cut(s) 37
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 4 cut(s) 354, 359, 486, 556
BlsI GCNGC 4 cut(s) 355, 360, 487, 557
BmcAI AGTACT 2 cut(s) 212, 548
BmeT110I CYCGRG 2 cut(s) 404, 550
BmiI GGNNCC 2 cut(s) 22, 158
BmsI GCATC 1 cut(s) 131
BpiI GAAGAC 2 cut(s) 507, 540
BplI GAGNNNNNCTC 2 cut(s) 598, 630
BpmI CTGGAG 1 cut(s) 39
Bpu10I CCTNAGC 1 cut(s) 6
BsaJI CCNNGG 2 cut(s) 77, 283
Bsc4I CCNNNNNNNGG 3 cut(s) 191, 512, 523
BseDI CCNNGG 2 cut(s) 77, 283
BseGI GGATG 2 cut(s) 122, 267
BseLI CCNNNNNNNGG 3 cut(s) 191, 512, 523
BseMII CTCAG 1 cut(s) 615
BseSI GKGCMC 1 cut(s) 77
BseXI GCAGC 3 cut(s) 370, 497, 567
Bsh1236I CGCG 1 cut(s) 356
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 2 cut(s) 288, 516
BshNI GGYRCC 1 cut(s) 156
BsiEI CGRYCG 1 cut(s) 133
BsiHKAI GWGCWC 2 cut(s) 399, 616
BsiHKCI CYCGRG 2 cut(s) 404, 550
BslFI GGGAC 2 cut(s) 449, 554
BslI CCNNNNNNNGG 3 cut(s) 191, 512, 523
BsmFI GGGAC 2 cut(s) 449, 554
BsnI GGCC 2 cut(s) 288, 516
BsoBI CYCGRG 2 cut(s) 404, 550
Bsp1286I GDGCHC 3 cut(s) 77, 399, 616
Bsp143I GATC 3 cut(s) 11, 364, 382
Bsp19I CCATGG 2 cut(s) 77, 283
BspACI CCGC 2 cut(s) 354, 620
BspANI GGCC 2 cut(s) 288, 516
BspCNI CTCAG 1 cut(s) 616
BspFNI CGCG 1 cut(s) 356
BspLI GGNNCC 2 cut(s) 22, 158
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 2 cut(s) 19, 372
BspT107I GGYRCC 1 cut(s) 156
BssECI CCNNGG 2 cut(s) 77, 283
BssMI GATC 3 cut(s) 11, 364, 382
BssT1I CCWWGG 2 cut(s) 77, 283
Bst4CI ACNGT 2 cut(s) 70, 131
BstC8I GCNNGC 1 cut(s) 600
BstDEI CTNAG 2 cut(s) 6, 624
BstDSI CCRYGG 2 cut(s) 77, 283
BstENI CCTNNNNNAGG 2 cut(s) 189, 521
BstF5I GGATG 2 cut(s) 122, 267
BstFNI CGCG 1 cut(s) 356
BstHHI GCGC 1 cut(s) 358
BstKTI GATC 3 cut(s) 14, 367, 385
BstMBI GATC 3 cut(s) 11, 364, 382
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 4 cut(s) 29, 81, 90, 119
BstSLI GKGCMC 1 cut(s) 77
BstUI CGCG 1 cut(s) 356
BstV1I GCAGC 3 cut(s) 370, 497, 567
BstV2I GAAGAC 2 cut(s) 507, 540
BstX2I RGATCY 1 cut(s) 364
BstYI RGATCY 1 cut(s) 364
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 2 cut(s) 288, 516
BtgI CCRYGG 2 cut(s) 77, 283
BtsCI GGATG 2 cut(s) 122, 267
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 1 cut(s) 600
CfoI GCGC 1 cut(s) 358
Csp6I GTAC 4 cut(s) 211, 409, 427, 547
CviAII CATG 3 cut(s) 78, 284, 380
CviQI GTAC 4 cut(s) 211, 409, 427, 547
DdeI CTNAG 2 cut(s) 6, 624
DpnI GATC 3 cut(s) 13, 366, 384
DpnII GATC 3 cut(s) 11, 364, 382
Ecl136II GAGCTC 1 cut(s) 397
Eco130I CCWWGG 2 cut(s) 77, 283
Eco24I GRGCYC 1 cut(s) 399
Eco53kI GAGCTC 1 cut(s) 397
Eco88I CYCGRG 2 cut(s) 404, 550
EcoICRI GAGCTC 1 cut(s) 397
EcoNI CCTNNNNNAGG 2 cut(s) 189, 521
EcoT14I CCWWGG 2 cut(s) 77, 283
EcoT38I GRGCYC 1 cut(s) 399
ErhI CCWWGG 2 cut(s) 77, 283
FaeI CATG 3 cut(s) 81, 287, 383
FaiI YATR 9 cut(s) 56, 79, 138, 216, 239, 285, 297, 381, 560
FalI AAGNNNNNCTT 4 cut(s) 180, 212, 522, 554
FaqI GGGAC 2 cut(s) 449, 554
FatI CATG 3 cut(s) 77, 283, 379
Fnu4HI GCNGC 4 cut(s) 354, 359, 486, 556
FokI GGATG 2 cut(s) 109, 274
FriOI GRGCYC 1 cut(s) 399
Fsp4HI GCNGC 4 cut(s) 354, 359, 486, 556
FspBI CTAG 2 cut(s) 326, 462
GlaI GCGC 1 cut(s) 357
GluI GCNGC 4 cut(s) 354, 359, 486, 556
GsuI CTGGAG 1 cut(s) 39
HaeIII GGCC 2 cut(s) 288, 516
HhaI GCGC 1 cut(s) 358
Hin1II CATG 3 cut(s) 81, 287, 383
Hin6I GCGC 1 cut(s) 356
HinP1I GCGC 1 cut(s) 356
HindIII AAGCTT 1 cut(s) 639
HinfI GANTC 3 cut(s) 100, 309, 567
HphI GGTGA 1 cut(s) 404
Hpy188I TCNGA 4 cut(s) 64, 235, 268, 278
Hpy188III TCNNGA 8 cut(s) 18, 104, 197, 306, 326, 452, 497, 634
Hpy99I CGWCG 1 cut(s) 47
HpyAV CCTTC 3 cut(s) 185, 460, 473
HpyCH4III ACNGT 2 cut(s) 70, 131
HpyCH4V TGCA 1 cut(s) 245
HpyF10VI GCNNNNNNNGC 4 cut(s) 29, 81, 90, 119
HpyF3I CTNAG 2 cut(s) 6, 624
Hsp92II CATG 3 cut(s) 81, 287, 383
HspAI GCGC 1 cut(s) 356
Kzo9I GATC 3 cut(s) 11, 364, 382
LmnI GCTCC 2 cut(s) 20, 482
Lsp1109I GCAGC 3 cut(s) 370, 497, 567
LweI GCATC 1 cut(s) 131
MaeI CTAG 2 cut(s) 326, 462
MaeIII GTNAC 2 cut(s) 332, 652
MalI GATC 3 cut(s) 13, 366, 384
MboI GATC 3 cut(s) 11, 364, 382
MboII GAAGA 4 cut(s) 46, 440, 512, 545
MflI RGATCY 1 cut(s) 364
MhlI GDGCHC 3 cut(s) 77, 399, 616
MluCI AATT 3 cut(s) 48, 57, 224
MlyI GAGTC 2 cut(s) 303, 576
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 4 cut(s) 90, 112, 299, 517
MroXI GAANNNNTTC 1 cut(s) 447
MseI TTAA 4 cut(s) 171, 528, 540, 585
MvnI CGCG 1 cut(s) 356
MwoI GCNNNNNNNGC 4 cut(s) 29, 81, 90, 119
NcoI CCATGG 2 cut(s) 77, 283
NdeII GATC 3 cut(s) 11, 364, 382
NlaIII CATG 3 cut(s) 81, 287, 383
NlaIV GGNNCC 2 cut(s) 22, 158
NmuCI GTSAC 1 cut(s) 332
PdmI GAANNNNTTC 1 cut(s) 447
PfeI GAWTC 1 cut(s) 100
PkrI GCNGC 4 cut(s) 355, 360, 487, 557
PleI GAGTC 2 cut(s) 303, 575
PpsI GAGTC 2 cut(s) 303, 575
PshBI ATTAAT 1 cut(s) 585
Psp124BI GAGCTC 1 cut(s) 399
PspN4I GGNNCC 2 cut(s) 22, 158
PsuI RGATCY 1 cut(s) 364
RsaI GTAC 4 cut(s) 212, 410, 428, 548
RsaNI GTAC 4 cut(s) 211, 409, 427, 547
SacI GAGCTC 1 cut(s) 399
SaqAI TTAA 4 cut(s) 171, 528, 540, 585
SatI GCNGC 4 cut(s) 354, 359, 486, 556
Sau3AI GATC 3 cut(s) 11, 364, 382
ScaI AGTACT 2 cut(s) 212, 548
SchI GAGTC 2 cut(s) 303, 576
SduI GDGCHC 3 cut(s) 77, 399, 616
SfaNI GCATC 1 cut(s) 131
Sse9I AATT 3 cut(s) 48, 57, 224
SsiI CCGC 2 cut(s) 354, 620
SspMI CTAG 2 cut(s) 326, 462
SstI GAGCTC 1 cut(s) 399
StyI CCWWGG 2 cut(s) 77, 283
TaaI ACNGT 2 cut(s) 70, 131
TaqI TCGA 4 cut(s) 45, 105, 133, 291
TasI AATT 3 cut(s) 48, 57, 224
TatI WGTACW 3 cut(s) 210, 426, 546
TauI GCSGC 1 cut(s) 356
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 4 cut(s) 171, 528, 540, 585
Tru9I TTAA 4 cut(s) 171, 528, 540, 585
TseFI GTSAC 1 cut(s) 332
TseI GCWGC 3 cut(s) 358, 485, 555
Tsp45I GTSAC 1 cut(s) 332
TspGWI ACGGA 1 cut(s) 579
VspI ATTAAT 1 cut(s) 585
XagI CCTNNNNNAGG 2 cut(s) 189, 521
XapI RAATTY 1 cut(s) 224
XbaI TCTAGA 1 cut(s) 325
XmnI GAANNNNTTC 1 cut(s) 447
XspI CTAG 2 cut(s) 326, 462
ZrmI AGTACT 2 cut(s) 212, 548
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.