Rroxscaffold_5G00335800

DSBA-like thioredoxin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000005
OG0005301
Physical Location & Seq
Reverse (-)
3219648 .. 3222400
2753 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_5G00335800.1

Sequence Viewer

Length: 381 bp
ATGGCCGAGGGATCAGCTGGAACCAAACCCGCAAAGAAGCTCGTCGAAATTGATATAATTTCGGATACCGTGTGCCCATGGTGCTTTGTGGGCAAGAGGAATCTCGACAAGGTCGTGGAAGCATCCAACGGTCGATATGACTTTAGGATGGCACCCATATCGCTTAATCCTAATGCCCCTAAAGAAGGTCTTAATAAGAGAAAGTACTATGAGCAAAAATTTGGGTCCGATATGTCCGCAAAACTGGGCAATAGGATGTCGAGGGTGGTGCGATTGCGACCCTTCTCAACCGACGACTTCCTCATCATGGGTGGTGAGTACACGGTTTTAGCATTGGCTAGTGGCGGCATGCCCTGTGGAGGCGGCTCTTGCCATCGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DSBA

Protein Analysis

126

Amino Acids

13.78

Weight (kDa)

9.3

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DSBA PF01323 17 - 74 5.9e-08 DSBA-like thioredoxin domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 151
AciI CCGC 4 cut(s) 30, 237, 345, 363
AclWI GGATC 1 cut(s) 19
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 218
AfaI GTAC 2 cut(s) 206, 320
AfiI CCNNNNNNNGG 3 cut(s) 185, 307, 359
AluBI AGCT 2 cut(s) 17, 40
AluI AGCT 2 cut(s) 17, 40
AlwI GGATC 1 cut(s) 19
AoxI GGCC 1 cut(s) 3
ApoI RAATTY 1 cut(s) 218
AspS9I GGNCC 1 cut(s) 225
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 225
BaeGI GKGCMC 1 cut(s) 77
BanI GGYRCC 1 cut(s) 151
BccI CCATC 1 cut(s) 142
BcgI CGANNNNNNTGC 4 cut(s) 141, 175, 250, 284
BciVI GTATCC 1 cut(s) 58
BfaI CTAG 1 cut(s) 339
BfuI GTATCC 1 cut(s) 58
BisI GCNGC 2 cut(s) 346, 364
BlsI GCNGC 2 cut(s) 347, 365
BmcAI AGTACT 1 cut(s) 206
Bme18I GGWCC 1 cut(s) 225
BmgT120I GGNCC 1 cut(s) 225
BmiI GGNNCC 3 cut(s) 22, 153, 226
BmrI ACTGGG 1 cut(s) 254
BmsI GCATC 1 cut(s) 131
BmuI ACTGGG 1 cut(s) 254
BsaJI CCNNGG 2 cut(s) 6, 77
Bsc4I CCNNNNNNNGG 3 cut(s) 185, 307, 359
Bse1I ACTGG 1 cut(s) 249
BseDI CCNNGG 2 cut(s) 6, 77
BseGI GGATG 3 cut(s) 122, 153, 261
BseLI CCNNNNNNNGG 3 cut(s) 185, 307, 359
BseNI ACTGG 1 cut(s) 249
BseSI GKGCMC 1 cut(s) 77
Bsh1285I CGRYCG 1 cut(s) 133
BshFI GGCC 1 cut(s) 5
BshNI GGYRCC 1 cut(s) 151
BsiEI CGRYCG 1 cut(s) 133
BslI CCNNNNNNNGG 3 cut(s) 185, 307, 359
BsnI GGCC 1 cut(s) 5
Bsp1286I GDGCHC 1 cut(s) 77
Bsp143I GATC 1 cut(s) 11
Bsp19I CCATGG 1 cut(s) 77
BspACI CCGC 4 cut(s) 30, 237, 345, 363
BspANI GGCC 1 cut(s) 5
BspLI GGNNCC 3 cut(s) 22, 153, 226
BspPI GGATC 1 cut(s) 19
BspT107I GGYRCC 1 cut(s) 151
BsrI ACTGG 1 cut(s) 249
BssECI CCNNGG 2 cut(s) 6, 77
BssMI GATC 1 cut(s) 11
BssT1I CCWWGG 1 cut(s) 77
Bst4CI ACNGT 3 cut(s) 70, 131, 325
BstC8I GCNNGC 1 cut(s) 350
BstDSI CCRYGG 1 cut(s) 77
BstENI CCTNNNNNAGG 1 cut(s) 183
BstF5I GGATG 3 cut(s) 122, 153, 261
BstKTI GATC 1 cut(s) 14
BstMBI GATC 1 cut(s) 11
BstMCI CGRYCG 1 cut(s) 133
BstMWI GCNNNNNNNGC 3 cut(s) 81, 90, 369
BstNSI RCATGY 1 cut(s) 352
BstSLI GKGCMC 1 cut(s) 77
BsuI GTATCC 1 cut(s) 58
BsuRI GGCC 1 cut(s) 5
BtgI CCRYGG 1 cut(s) 77
BtsCI GGATG 3 cut(s) 122, 153, 261
Cac8I GCNNGC 1 cut(s) 350
Cfr13I GGNCC 1 cut(s) 225
Csp6I GTAC 2 cut(s) 205, 319
CviAII CATG 3 cut(s) 78, 307, 349
CviJI RGCY 5 cut(s) 5, 17, 40, 338, 366
CviKI_1 RGCY 5 cut(s) 5, 17, 40, 338, 366
CviQI GTAC 2 cut(s) 205, 319
DpnI GATC 1 cut(s) 13
DpnII GATC 1 cut(s) 11
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 77
Eco47I GGWCC 1 cut(s) 225
EcoNI CCTNNNNNAGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 77
ErhI CCWWGG 1 cut(s) 77
FaeI CATG 3 cut(s) 81, 310, 352
FaiI YATR 8 cut(s) 56, 79, 138, 158, 210, 233, 308, 350
FalI AAGNNNNNCTT 2 cut(s) 174, 206
FatI CATG 3 cut(s) 77, 306, 348
FauI CCCGC 1 cut(s) 37
Fnu4HI GCNGC 2 cut(s) 346, 364
FokI GGATG 3 cut(s) 109, 160, 268
Fsp4HI GCNGC 2 cut(s) 346, 364
FspBI CTAG 1 cut(s) 339
GluI GCNGC 2 cut(s) 346, 364
HaeIII GGCC 1 cut(s) 5
Hin1II CATG 3 cut(s) 81, 310, 352
HinfI GANTC 1 cut(s) 100
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 321
Hpy188I TCNGA 2 cut(s) 64, 229
Hpy188III TCNNGA 1 cut(s) 104
Hpy8I GTNNAC 1 cut(s) 321
Hpy99I CGWCG 2 cut(s) 47, 296
HpyAV CCTTC 2 cut(s) 179, 292
HpyCH4III ACNGT 3 cut(s) 70, 131, 325
HpyF10VI GCNNNNNNNGC 3 cut(s) 81, 90, 369
Hsp92II CATG 3 cut(s) 81, 310, 352
Kzo9I GATC 1 cut(s) 11
LpnPI CCDG 3 cut(s) 3, 230, 367
LweI GCATC 1 cut(s) 131
MaeI CTAG 1 cut(s) 339
MalI GATC 1 cut(s) 13
MboI GATC 1 cut(s) 11
MhlI GDGCHC 1 cut(s) 77
MluCI AATT 3 cut(s) 48, 57, 218
MmeI TCCRAC 1 cut(s) 150
MnlI CCTC 4 cut(s) 90, 255, 311, 353
MseI TTAA 2 cut(s) 165, 192
MspA1I CMGCKG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 81, 90, 369
NcoI CCATGG 1 cut(s) 77
NdeII GATC 1 cut(s) 11
NlaIII CATG 3 cut(s) 81, 310, 352
NlaIV GGNNCC 3 cut(s) 22, 153, 226
NmeAIII GCCGAG 1 cut(s) 31
NspI RCATGY 1 cut(s) 352
PaeI GCATGC 1 cut(s) 352
PcsI WCGNNNNNNNCGW 1 cut(s) 111
PfeI GAWTC 1 cut(s) 100
PflFI GACNNNGTC 1 cut(s) 110
PkrI GCNGC 2 cut(s) 347, 365
PspN4I GGNNCC 3 cut(s) 22, 153, 226
PspPI GGNCC 1 cut(s) 225
PsyI GACNNNGTC 1 cut(s) 110
PvuII CAGCTG 1 cut(s) 17
RsaI GTAC 2 cut(s) 206, 320
RsaNI GTAC 2 cut(s) 205, 319
SaqAI TTAA 2 cut(s) 165, 192
SatI GCNGC 2 cut(s) 346, 364
Sau3AI GATC 1 cut(s) 11
Sau96I GGNCC 1 cut(s) 225
ScaI AGTACT 1 cut(s) 206
SduI GDGCHC 1 cut(s) 77
SetI ASST 4 cut(s) 19, 42, 114, 190
SfaNI GCATC 1 cut(s) 131
SinI GGWCC 1 cut(s) 225
SphI GCATGC 1 cut(s) 352
Sse9I AATT 3 cut(s) 48, 57, 218
SsiI CCGC 4 cut(s) 30, 237, 345, 363
SspMI CTAG 1 cut(s) 339
StyI CCWWGG 1 cut(s) 77
TaaI ACNGT 3 cut(s) 70, 131, 325
TaqI TCGA 4 cut(s) 45, 105, 133, 260
TasI AATT 3 cut(s) 48, 57, 218
TatI WGTACW 2 cut(s) 204, 318
TauI GCSGC 2 cut(s) 348, 366
TfiI GAWTC 1 cut(s) 100
Tru1I TTAA 2 cut(s) 165, 192
Tru9I TTAA 2 cut(s) 165, 192
Tth111I GACNNNGTC 1 cut(s) 110
VpaK11BI GGWCC 1 cut(s) 225
XagI CCTNNNNNAGG 1 cut(s) 183
XapI RAATTY 1 cut(s) 218
XceI RCATGY 1 cut(s) 352
XspI CTAG 1 cut(s) 339
ZrmI AGTACT 1 cut(s) 206
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.