FvH4_6g53851

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003475
Physical Location & Seq
Reverse (-)
39417418 .. 39424683
7266 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g53851.t2

Sequence Viewer

Length: 4461 bp
ATGATGGCCCACGGCAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGGCGATCTTCTCCTCCTCCTCCGCCAAAGCAGATGAAGCCTCGGAAGGAAATGCTGGGGAGAAGGCTCTAAAAGGGTCACTTGATGAGTCAAAAGATCACCTAGCATCTGAGAGCAGCATACCTTTGTCTCCTCAATGGCTGCATGCTAAGGCAGTTGATGCTAAGCCACTCACAAGTGGAACATCAGGGGAAATCCATGCACCAGGGTCTTTGTCTCATGGTAACTCTACCGATTCCAATACAAGAGACGTGTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACTGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTACTGAGAATAGAGCAGAGGATCACCGAGCAGGTGGCCCGTCTACTAGAGATGTTACTGAGAATAAAATTTTGTCTTCTGACCGCTGGCATGAATCGCGGAGAGACAATAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGATTCTCGTATAGAGAAAACAGATCTTGAGAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCGGTTCCTGAACGTGATGGTGATTCTCGTGATAAGTGGAGGCCACGTCATCGCATGGAGGTTCAATCTGGGGGGCCAGCGCCATACCGTGCCGCACCAGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGACGAGGGAGGTCCCATACTAGTGGTAGTGTACAGATTGGAAGACCTTCCTCTATATCTACTATTGGGTCTGTTCTGGATCAAAATCAAAGTGTGTTGGGAAAAGCTGGCCCCTTTACTGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTCGATATTTATCGTAAGCAAAAGATAGAATCAACTTTCGATATTGTGCCTGAAGGGATGGAGCATGTCTCTCCAATAACCCAAGTGGGCTGTATTGAGCCATTAGCTTTTGTCGCACCTGATGCAGATGAAGAGGCTGGTATGGAAGATATTTGGAAGGGAAGAATCACCAGCAGTGATGATGGAATTAGTAATGCCTCTTTAAAAAAGGAGGAAGATAAAAAGATTTCATCACAGTCTGAAGAAAATGCGCTGTCTACTGGAGAGGTCTCAACTATTTGTGGCCCGCAAACACAAACTTTGAATGAAGGGGTTGAAGGCGGGCACAGAGTTCCTATTGGCACAGCAATTGCTGATGGATCGAATCTGGTAGCTCCAAAGAACTGTGATCACAAAAGTGCTACGGAGACTGATGGCCTCAGTAATAATGTTGATGAACTAAGAATTTCTGGATGCCATGATGTGAGAGATTTCAGCCTCACAAAGCATTCTATGTCGGAATATTATGAAGCAGCTACTAATGTGACTGGTAGCCAGCTTCCTGATTCAAGTTCACCATTTCATATTCCATCTCTGCAGCAAGCCTCAAGTAGTGATCATTTCAAGAGCAATGAGAAGGCAGATCTGGAAAGGGTTGTCTCATTTGAGGATTTAAGTTTATGCTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGACATAATTCAATGGTTTGATCAAGGATTTTTTGGGACTGACTTACTGTTGCGCTTGACTGATGCCCCTGATGGTTCTCCTTTTCAAGAACTCGGTGACATGATGCCGAATCTGAAAACTCAATCCGGATCGGTCTCCAACTCCAATTTGCCTACCAACATAGAATCGTTGGATGGTGTTGGAAGCATGTTGGAAGAGAGGAAAACTGCTCCTCACTATGAGGAGTCTAACAATCTAAATATTCAACATTGGACTCCATCTGGACTTGAACCTACCTCAAGTGATGATTTGCACTCAAGAATACGCAATAACAATTACAACTTCGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTACGTTGAAAGAGATGAAGAAATAGTATATCCTGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCATGGCTCATTTTCAAATTCCCCAAGTGTTCCTTCCCTTTCAGATGAAGTTTCCGAAAGCAACCTACCTAATTGCCAGGATGATAAGTTACACCCTTTCGGCCTGTTGATGTCTGAGCTCAGAGGCAACTCTCATATAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCACAGTTTAGTGATACCTTGTTTGAGGGAGGCATATCTAAACTTAATTGGTTGAATAATCCTAATATCCCAATGCATCAAGTGGTGCCTGACATGGAGCATCTAATAGAACTACAGCGTCAAGAACAGCAGCAGCATCGCTTATTGGAGCTTCAGCAACAACGGCAGCTGGAGTTTCAACGTCAGCAGCAGCACGAGTTGGAGTTTCAACAGCAGCGCCAACTCCAGCAACAGCGCCAGTTGGAGCTACAGCAGCAGCGTCAGTTGGAACTTCACCTTCAGCAGCAGCACCAGTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTTCAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTGGAGCAGCAGCAGCAACAGCAATACCATTTGGAGCTTCAGCAGCAGCGGAAGCTGGAGCAGCAGCTGCAGCGTCTTCAACAACAGAGGCAGTTGGAGTTTCAGCAACAGCAGCGTCACTTAGAGCTTCAGCAACAGCAGCGTCAATTGGAGCTTCAGCCTCAGCACCAATTGGAGCAGCACCTTCGCCATCACCAAAATTTGCCGCAGCACCAGCAGTCCCAAGCCGAACAATTGCTCCTTGAACAGTTATTGAGTCGACAGATGCCTGGTAGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAACCTTCATGATCAGTTACACCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACAATCCCTCGCTTGAGCAGATCATCCAAGCAACTGTGGGCGAGGGTGCCCTCCCAGTACATTCTGATTTTTTTGACCTCATATCTCAGGCCAAGCTAGCAAATAGGAACACATCAGAGCTGCCACTCCGCCTTAATGAACAAGAGTTGCAGGCACAACAAATATCTATGGCATTGAGAGAGCAGCAGATGGAAGGGGAAAGGCGTGTTGGTGGGCCTTGGTTTGCAGATGAAGCAGGTCCTTTTGTGAGGGATCCTGCTGGGCATCACCAGGCTCACATGTTGGGACATAATTCTTCAGAAAATTACCAGAAACAGCAGAGGTTTCCATCCAATGAACAGGAACTAAGCCATCGCAATTGGAATCGGGCCTCTCAGGAGAGACCTCAGGGAGGATTCTATGAACCTAACCCTACGGAATTTGATAGGTTCTCAGTTCCTGCGGGTTCTCATGGGATGAACTTGGATATTGCAAACATATGTGGACAAGATCTAGATGTGCACGAACAATATCAGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCTTCCAGAGGTCAGGGAGGCTCCGTACATCTTCCTTTTAATGTTCTTCCAGATCAGTCAGTTGGTATGAACAACTCCTTCACAGAAGGCCCACATAATTCAAATTATAGCATCTTACTGCAAGACCAGTTGGGTAGTTATGGTATGAACGAGCAAGCAAGTAATATGGCAAGAAGATCTGATTCTAGAGCATTTATGGACCCTCAATTCTTGTCAGGAACCAGGGATACTCCTCATGAAGGCAACATGGTGGAGCAACAAAACACAGCCATTGATAGCGAGGAACTACCTAGTAATGCCCATAGCAGGCATAGCTCGCTGAGCAGCGCTGGTGGATGTGGGGGTTTTCACAGCTCTGAGATGGGAGTAGATAAATCACTTGGAGAAGATGTTCATGATGGAAGGCTGCCTTCTACTACAACCAAAGGGTTTGACAGTGCTACATCGAGACGCCGCGTGTTATCTTCCCAGGATGTTTTGTCTGAGTCTGCACTCTCTCTACCTGTTAAGCCAAGAAGCTCGACAAGCCTCAGAACTTCTGAAGGAAAACCTGCTGCCACACCGGTCTCTGAATCTCTAGCAACTAGCAAAAATCATGCACAGTTCAGGAGGACTTCATCTTACAGTGATGCTGGTGTCTCCGAGACATCCTTCATGGATGTGCTCAAGAAACCAGTTATTCCAGAGGCTGATAACAGGGGTGCAATGGAGTCATCTGATGGAGGTGGCCAAGTGGGCCGAACTGGCAAGAAGAAAGGCAAGAAAGGAAGACAGATCGATCCTACTCTCCTAGGCTTTAAGGTATCCAGCAACCGGATCATGATGGGTGAGATCCATGGCCTTGATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1487

Amino Acids

166.61

Weight (kDa)

5.61

Isoelectric Point (pI)

61.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 554 - 598 6.1e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 488
Acc36I ACCTGC 4 cut(s) 488, 2296, 3287, 4168
AccB1I GGYRCC 2 cut(s) 2413, 3107
AccB7I CCANNNNNTGG 1 cut(s) 797
AccI GTMKAC 3 cut(s) 509, 1247, 2953
AccII CGCG 2 cut(s) 565, 4065
AccIII TCCGGA 1 cut(s) 1823
AcoI YGGCCR 2 cut(s) 804, 4336
AcsI RAATTY 5 cut(s) 534, 1434, 2161, 2893, 3479
AcyI GRCGYC 1 cut(s) 4060
AfaI GTAC 4 cut(s) 873, 2033, 3120, 3636
AfeI AGCGCT 1 cut(s) 3937
AfiI CCNNNNNNNGG 7 cut(s) 49, 797, 1691, 3452, 3848, 3915, 4422
AflIII ACRYGT 2 cut(s) 300, 3339
AgeI ACCGGT 1 cut(s) 4171
AhdI GACNNNNNGTC 1 cut(s) 670
AhlI ACTAGT 1 cut(s) 860
AjiI CACGTC 2 cut(s) 301, 740
AjuI GAANNNNNNNTTGG 4 cut(s) 2161, 2193, 3252, 3284
AleI CACNNNNGTG 1 cut(s) 1386
AloI GAACNNNNNNTCC 4 cut(s) 2916, 2948, 3984, 4016
Alw21I GWGCWC 3 cut(s) 2265, 3564, 4275
Alw44I GTGCAC 1 cut(s) 3560
AlwNI CAGNNNCTG 3 cut(s) 2761, 3500, 4298
Ama87I CYCGRG 1 cut(s) 321
Aor13HI TCCGGA 1 cut(s) 1823
Aor51HI AGCGCT 1 cut(s) 3937
ApaI GGGCCC 1 cut(s) 599
ApaLI GTGCAC 1 cut(s) 3560
ApoI RAATTY 5 cut(s) 534, 1434, 2161, 2893, 3479
AsiGI ACCGGT 1 cut(s) 4171
Asp700I GAANNNNTTC 2 cut(s) 886, 3999
AspA2I CCTAGG 1 cut(s) 4399
AspLEI GCGC 8 cut(s) 775, 1243, 1752, 2547, 2565, 2667, 2691, 3938
AsuII TTCGAA 1 cut(s) 2022
AsuNHI GCTAGC 1 cut(s) 3157
AvaI CYCGRG 1 cut(s) 321
AvaII GGWCC 4 cut(s) 852, 1682, 3299, 3808
AvrII CCTAGG 1 cut(s) 4399
AxyI CCTNAGG 1 cut(s) 3447
BaeGI GKGCMC 4 cut(s) 599, 1317, 3112, 3564
BaeI ACNNNNGTAYC 2 cut(s) 2015, 2048
BalI TGGCCA 2 cut(s) 806, 4338
BamHI GGATCC 1 cut(s) 3313
BanI GGYRCC 2 cut(s) 2413, 3107
BanII GRGCYC 2 cut(s) 599, 2265
BarI GAAGNNNNNNTAC 2 cut(s) 956, 988
BauI CACGAG 3 cut(s) 720, 2522, 2997
BbsI GAAGAC 4 cut(s) 534, 889, 2762, 4384
Bbv12I GWGCWC 3 cut(s) 2265, 3564, 4275
BbvCI CCTCAGC 1 cut(s) 2856
BceAI ACGGC 3 cut(s) 28, 423, 2507
BcgI CGANNNNNNTGC 4 cut(s) 2447, 2481, 2861, 2895
BciVI GTATCC 2 cut(s) 3829, 4423
BclI TGATCA 5 cut(s) 1378, 1585, 1717, 2323, 3013
BcuI ACTAGT 1 cut(s) 860
BfmI CTRYAG 6 cut(s) 1565, 2441, 2576, 2660, 2678, 2762
BfoI RGCGCY 6 cut(s) 776, 2548, 2566, 2668, 2692, 3939
BfuAI ACCTGC 4 cut(s) 488, 2296, 3287, 4168
BfuI GTATCC 2 cut(s) 3829, 4423
BglI GCCNNNNNGGC 2 cut(s) 4344, 4353
BglII AGATCT 5 cut(s) 34, 637, 1612, 3550, 3785
BlnI CCTAGG 1 cut(s) 4399
BlpI GCTNAGC 2 cut(s) 213, 3929
Bme18I GGWCC 4 cut(s) 852, 1682, 3299, 3808
BmeRI GACNNNNNGTC 1 cut(s) 670
BmeT110I CYCGRG 1 cut(s) 321
BmgBI CACGTC 2 cut(s) 301, 740
BmrI ACTGGG 1 cut(s) 3110
BmtI GCTAGC 1 cut(s) 3161
BmuI ACTGGG 1 cut(s) 3110
BpiI GAAGAC 4 cut(s) 534, 889, 2762, 4384
BplI GAGNNNNNCTC 2 cut(s) 1043, 1075
BpmI CTGGAG 5 cut(s) 1272, 2012, 2519, 2537, 2771
Bpu10I CCTNAGC 2 cut(s) 198, 2856
Bpu1102I GCTNAGC 2 cut(s) 213, 3929
Bpu14I TTCGAA 1 cut(s) 2022
BpuEI CTTGAG 9 cut(s) 15, 662, 1561, 1650, 1960, 1978, 2277, 3095, 4259
Bsa29I ATCGAT 1 cut(s) 4386
BsaBI GATNNNNATC 3 cut(s) 924, 999, 3607
BsaHI GRCGYC 1 cut(s) 4060
BsaI GGTCTC 5 cut(s) 1264, 1837, 2122, 3436, 4180
BsaWI WCCGGW 3 cut(s) 1823, 4171, 4422
BsaXI ACNNNNNCTCC 8 cut(s) 724, 754, 2916, 2946, 3618, 3648, 3984, 4014
Bsc4I CCNNNNNNNGG 7 cut(s) 49, 797, 1691, 3452, 3848, 3915, 4422
Bse118I RCCGGY 2 cut(s) 49, 4171
Bse21I CCTNAGG 1 cut(s) 3447
Bse3DI GCAATG 2 cut(s) 1606, 4320
Bse8I GATNNNNATC 3 cut(s) 924, 999, 3607
BseAI TCCGGA 1 cut(s) 1823
BseCI ATCGAT 1 cut(s) 4386
BseJI GATNNNNATC 3 cut(s) 924, 999, 3607
BseLI CCNNNNNNNGG 7 cut(s) 49, 797, 1691, 3452, 3848, 3915, 4422
BseMI GCAATG 2 cut(s) 1606, 4320
BseSI GKGCMC 4 cut(s) 599, 1317, 3112, 3564
BseYI CCCAGC 2 cut(s) 104, 3320
BsgI GTGCAG 1 cut(s) 4083
Bsh1236I CGCG 2 cut(s) 565, 4065
BshNI GGYRCC 2 cut(s) 2413, 3107
BshTI ACCGGT 1 cut(s) 4171
BshVI ATCGAT 1 cut(s) 4386
BsiHKAI GWGCWC 3 cut(s) 2265, 3564, 4275
BsiHKCI CYCGRG 1 cut(s) 321
BsiSI CCGG 4 cut(s) 50, 1824, 4172, 4423
BslFI GGGAC 6 cut(s) 338, 838, 1695, 1747, 2899, 3360
BslI CCNNNNNNNGG 7 cut(s) 49, 797, 1691, 3452, 3848, 3915, 4422
BsmBI CGTCTC 2 cut(s) 291, 4051
BsmFI GGGAC 6 cut(s) 338, 838, 1695, 1747, 2899, 3360
BsmI GAATGC 2 cut(s) 352, 1477
Bso31I GGTCTC 5 cut(s) 1264, 1837, 2122, 3436, 4180
BsoBI CYCGRG 1 cut(s) 321
Bsp119I TTCGAA 1 cut(s) 2022
Bsp120I GGGCCC 1 cut(s) 595
Bsp1286I GDGCHC 6 cut(s) 599, 1317, 2265, 3112, 3564, 4275
Bsp13I TCCGGA 1 cut(s) 1823
Bsp1407I TGTACA 1 cut(s) 871
Bsp1720I GCTNAGC 2 cut(s) 213, 3929
Bsp19I CCATGG 1 cut(s) 4444
BspDI ATCGAT 1 cut(s) 4386
BspEI TCCGGA 1 cut(s) 1823
BspFNI CGCG 2 cut(s) 565, 4065
BspHI TCATGA 4 cut(s) 3010, 3844, 4003, 4428
BspMAI CTGCAG 3 cut(s) 1569, 2664, 2766
BspMI ACCTGC 4 cut(s) 488, 2296, 3287, 4168
BspOI GCTAGC 1 cut(s) 3161
BspT104I TTCGAA 1 cut(s) 2022
BspT107I GGYRCC 2 cut(s) 2413, 3107
BspTNI GGTCTC 5 cut(s) 1264, 1837, 2122, 3436, 4180
BsrDI GCAATG 2 cut(s) 1606, 4320
BsrFI RCCGGY 2 cut(s) 49, 4171
BsrGI TGTACA 1 cut(s) 871
BssAI RCCGGY 2 cut(s) 49, 4171
BssNI GRCGYC 1 cut(s) 4060
BssSI CACGAG 3 cut(s) 720, 2522, 2997
BssT1I CCWWGG 3 cut(s) 3278, 4399, 4444
Bst2BI CACGAG 3 cut(s) 720, 2522, 2997
Bst6I CTCTTC 2 cut(s) 1116, 1887
BstACI GRCGYC 1 cut(s) 4060
BstAPI GCANNNNNTGC 3 cut(s) 209, 1112, 2761
BstAUI TGTACA 1 cut(s) 871
BstBI TTCGAA 1 cut(s) 2022
BstDSI CCRYGG 2 cut(s) 10, 4444
BstENI CCTNNNNNAGG 2 cut(s) 3450, 3846
BstFNI CGCG 2 cut(s) 565, 4065
BstH2I RGCGCY 6 cut(s) 776, 2548, 2566, 2668, 2692, 3939
BstHHI GCGC 8 cut(s) 775, 1243, 1752, 2547, 2565, 2667, 2691, 3938
BstNSI RCATGY 4 cut(s) 197, 1058, 1888, 3343
BstSFI CTRYAG 6 cut(s) 1565, 2441, 2576, 2660, 2678, 2762
BstSLI GKGCMC 4 cut(s) 599, 1317, 3112, 3564
BstUI CGCG 2 cut(s) 565, 4065
BstV2I GAAGAC 4 cut(s) 534, 889, 2762, 4384
BstX2I RGATCY 8 cut(s) 34, 637, 1612, 3313, 3550, 3602, 3785, 4440
BstXI CCANNNNNNTGG 1 cut(s) 863
BstYI RGATCY 8 cut(s) 34, 637, 1612, 3313, 3550, 3602, 3785, 4440
Bsu15I ATCGAT 1 cut(s) 4386
Bsu36I CCTNAGG 1 cut(s) 3447
BsuI GTATCC 2 cut(s) 3829, 4423
BsuTUI ATCGAT 1 cut(s) 4386
BtgI CCRYGG 2 cut(s) 10, 4444
BtgZI GCGATG 3 cut(s) 728, 2450, 3398
BtrI CACGTC 2 cut(s) 301, 740
BtsI GCAGTG 3 cut(s) 1171, 2116, 2984
BtsIMutI CAGTG 5 cut(s) 1171, 2116, 2984, 4051, 4240
BveI ACCTGC 4 cut(s) 488, 2296, 3287, 4168
CaiI CAGNNNCTG 3 cut(s) 2761, 3500, 4298
CciI TCATGA 4 cut(s) 3010, 3844, 4003, 4428
CfoI GCGC 8 cut(s) 775, 1243, 1752, 2547, 2565, 2667, 2691, 3938
Cfr10I RCCGGY 2 cut(s) 49, 4171
ClaI ATCGAT 1 cut(s) 4386
CseI GACGC 6 cut(s) 2435, 2576, 2756, 2798, 2825, 4068
Csp6I GTAC 4 cut(s) 872, 2032, 3119, 3635
CspAI ACCGGT 1 cut(s) 4171
CviQI GTAC 4 cut(s) 872, 2032, 3119, 3635
DraI TTTAAA 1 cut(s) 1194
DriI GACNNNNNGTC 1 cut(s) 670
EaeI YGGCCR 2 cut(s) 804, 4336
Eam1104I CTCTTC 2 cut(s) 1116, 1887
Eam1105I GACNNNNNGTC 1 cut(s) 670
EarI CTCTTC 2 cut(s) 1116, 1887
EciI GGCGGA 2 cut(s) 61, 3179
Ecl136II GAGCTC 1 cut(s) 2263
Eco130I CCWWGG 3 cut(s) 3278, 4399, 4444
Eco147I AGGCCT 1 cut(s) 2098
Eco24I GRGCYC 2 cut(s) 599, 2265
Eco31I GGTCTC 5 cut(s) 1264, 1837, 2122, 3436, 4180
Eco47I GGWCC 4 cut(s) 852, 1682, 3299, 3808
Eco47III AGCGCT 1 cut(s) 3937
Eco53kI GAGCTC 1 cut(s) 2263
Eco81I CCTNAGG 1 cut(s) 3447
Eco88I CYCGRG 1 cut(s) 321
EcoICRI GAGCTC 1 cut(s) 2263
EcoNI CCTNNNNNAGG 2 cut(s) 3450, 3846
EcoO109I RGGNCCY 5 cut(s) 595, 596, 852, 1682, 3299
EcoT14I CCWWGG 3 cut(s) 3278, 4399, 4444
EcoT22I ATGCAT 1 cut(s) 2406
EcoT38I GRGCYC 2 cut(s) 599, 2265
ErhI CCWWGG 3 cut(s) 3278, 4399, 4444
Esp3I CGTCTC 2 cut(s) 291, 4051
FalI AAGNNNNNCTT 6 cut(s) 114, 146, 2161, 2193, 4003, 4035
FaqI GGGAC 6 cut(s) 338, 838, 1695, 1747, 2899, 3360
FauI CCCGC 3 cut(s) 1284, 1304, 3496
FauNDI CATATG 1 cut(s) 3539
FbaI TGATCA 5 cut(s) 1378, 1585, 1717, 2323, 3013
FblI GTMKAC 3 cut(s) 509, 1247, 2953
FriOI GRGCYC 2 cut(s) 599, 2265
GlaI GCGC 8 cut(s) 774, 1242, 1751, 2546, 2564, 2666, 2690, 3937
GsaI CCCAGC 2 cut(s) 108, 3324
GsuI CTGGAG 5 cut(s) 1272, 2012, 2519, 2537, 2771
HaeII RGCGCY 6 cut(s) 776, 2548, 2566, 2668, 2692, 3939
HapII CCGG 4 cut(s) 50, 1824, 4172, 4423
HgaI GACGC 6 cut(s) 2435, 2576, 2756, 2798, 2825, 4068
HhaI GCGC 8 cut(s) 775, 1243, 1752, 2547, 2565, 2667, 2691, 3938
Hin1I GRCGYC 1 cut(s) 4060
Hin6I GCGC 8 cut(s) 773, 1241, 1750, 2545, 2563, 2665, 2689, 3936
HinP1I GCGC 8 cut(s) 773, 1241, 1750, 2545, 2563, 2665, 2689, 3936
HincII GTYRAC 3 cut(s) 367, 664, 2954
HindII GTYRAC 3 cut(s) 367, 664, 2954
HindIII AAGCTT 1 cut(s) 2295
HpaI GTTAAC 1 cut(s) 367
HpaII CCGG 4 cut(s) 50, 1824, 4172, 4423
Hpy166II GTNNAC 9 cut(s) 367, 510, 664, 872, 1248, 1544, 2954, 3545, 3562
Hpy8I GTNNAC 9 cut(s) 367, 510, 664, 872, 1248, 1544, 2954, 3545, 3562
Hpy99I CGWCG 1 cut(s) 375
HpyCH4IV ACGT 5 cut(s) 300, 706, 739, 2061, 2509
HpySE526I ACGT 5 cut(s) 300, 706, 739, 2061, 2509
Hsp92I GRCGYC 1 cut(s) 4060
HspAI GCGC 8 cut(s) 773, 1241, 1750, 2545, 2563, 2665, 2689, 3936
Kpn2I TCCGGA 1 cut(s) 1823
KroI GCCGGC 1 cut(s) 49
KroNI GCCGGC 1 cut(s) 51
Ksp22I TGATCA 5 cut(s) 1378, 1585, 1717, 2323, 3013
KspAI GTTAAC 1 cut(s) 367
MaeII ACGT 5 cut(s) 300, 706, 739, 2061, 2509
MfeI CAATTG 5 cut(s) 1338, 2840, 2864, 2927, 3418
MflI RGATCY 8 cut(s) 34, 637, 1612, 3313, 3550, 3602, 3785, 4440
MhlI GDGCHC 6 cut(s) 599, 1317, 2265, 3112, 3564, 4275
MlsI TGGCCA 2 cut(s) 806, 4338
MluNI TGGCCA 2 cut(s) 806, 4338
MlyI GAGTC 7 cut(s) 146, 456, 1931, 1945, 2959, 4103, 4328
Mox20I TGGCCA 2 cut(s) 806, 4338
Mph1103I ATGCAT 1 cut(s) 2406
MreI CGCCGGCG 1 cut(s) 49
MroI TCCGGA 1 cut(s) 1823
MroNI GCCGGC 1 cut(s) 49
MroXI GAANNNNTTC 2 cut(s) 886, 3999
MscI TGGCCA 2 cut(s) 806, 4338
MseI TTAA 9 cut(s) 366, 1193, 1643, 2373, 3195, 3579, 3648, 4116, 4407
MslI CAYNNNNRTG 5 cut(s) 589, 861, 1386, 3027, 4268
Msp20I TGGCCA 2 cut(s) 806, 4338
MspA1I CMGCKG 6 cut(s) 552, 2138, 2497, 2659, 2743, 2761
MspI CCGG 4 cut(s) 50, 1824, 4172, 4423
MunI CAATTG 5 cut(s) 1338, 2840, 2864, 2927, 3418
Mva1269I GAATGC 2 cut(s) 352, 1477
MvnI CGCG 2 cut(s) 565, 4065
NaeI GCCGGC 1 cut(s) 51
NcoI CCATGG 1 cut(s) 4444
NdeI CATATG 1 cut(s) 3539
NgoMIV GCCGGC 1 cut(s) 49
NheI GCTAGC 1 cut(s) 3157
NmuCI GTSAC 5 cut(s) 126, 448, 1513, 1793, 2810
NsiI ATGCAT 1 cut(s) 2406
NspI RCATGY 4 cut(s) 197, 1058, 1888, 3343
NspV TTCGAA 1 cut(s) 2022
OliI CACNNNNGTG 1 cut(s) 1386
PaeI GCATGC 1 cut(s) 197
PagI TCATGA 4 cut(s) 3010, 3844, 4003, 4428
PaqCI CACCTGC 1 cut(s) 488
PasI CCCWGGG 1 cut(s) 978
PceI AGGCCT 1 cut(s) 2098
PciI ACATGT 1 cut(s) 3339
PctI GAATGC 2 cut(s) 352, 1477
PdiI GCCGGC 1 cut(s) 51
PdmI GAANNNNTTC 2 cut(s) 886, 3999
PflMI CCANNNNNTGG 1 cut(s) 797
PfoI TCCNGGA 1 cut(s) 2089
PinAI ACCGGT 1 cut(s) 4171
PleI GAGTC 7 cut(s) 145, 455, 1930, 1945, 2958, 4102, 4327
PpsI GAGTC 7 cut(s) 145, 455, 1930, 1945, 2958, 4102, 4327
PpuMI RGGWCCY 3 cut(s) 852, 1682, 3299
PscI ACATGT 1 cut(s) 3339
Psp124BI GAGCTC 1 cut(s) 2265
Psp5II RGGWCCY 3 cut(s) 852, 1682, 3299
PspFI CCCAGC 2 cut(s) 104, 3320
PspOMI GGGCCC 1 cut(s) 595
PspPPI RGGWCCY 3 cut(s) 852, 1682, 3299
PsrI GAACNNNNNNTAC 2 cut(s) 3820, 3852
PstI CTGCAG 3 cut(s) 1569, 2664, 2766
PstNI CAGNNNCTG 3 cut(s) 2761, 3500, 4298
PsuI RGATCY 8 cut(s) 34, 637, 1612, 3313, 3550, 3602, 3785, 4440
PvuII CAGCTG 4 cut(s) 2138, 2497, 2659, 2761
RsaI GTAC 4 cut(s) 873, 2033, 3120, 3636
RsaNI GTAC 4 cut(s) 872, 2032, 3119, 3635
RseI CAYNNNNRTG 5 cut(s) 589, 861, 1386, 3027, 4268
SacI GAGCTC 1 cut(s) 2265
SalI GTCGAC 1 cut(s) 2952
SaqAI TTAA 9 cut(s) 366, 1193, 1643, 2373, 3195, 3579, 3648, 4116, 4407
SchI GAGTC 7 cut(s) 146, 456, 1931, 1945, 2959, 4103, 4328
SduI GDGCHC 6 cut(s) 599, 1317, 2265, 3112, 3564, 4275
SfcI CTRYAG 6 cut(s) 1565, 2441, 2576, 2660, 2678, 2762
SfiI GGCCNNNNNGGCC 1 cut(s) 4344
SfuI TTCGAA 1 cut(s) 2022
SgrAI CRCCGGYG 1 cut(s) 49
SinI GGWCC 4 cut(s) 852, 1682, 3299, 3808
SmiMI CAYNNNNRTG 5 cut(s) 589, 861, 1386, 3027, 4268
SmlI CTYRAG 9 cut(s) 30, 641, 1576, 1665, 1975, 1993, 2292, 3074, 4274
SmoI CTYRAG 9 cut(s) 30, 641, 1576, 1665, 1975, 1993, 2292, 3074, 4274
SpeI ACTAGT 1 cut(s) 860
SphI GCATGC 1 cut(s) 197
SseBI AGGCCT 1 cut(s) 2098
SspI AATATT 2 cut(s) 1493, 1939
SstI GAGCTC 1 cut(s) 2265
StuI AGGCCT 1 cut(s) 2098
StyI CCWWGG 3 cut(s) 3278, 4399, 4444
TaiI ACGT 5 cut(s) 303, 709, 742, 2064, 2512
TaqI TCGA 9 cut(s) 993, 1029, 1352, 1698, 2022, 2953, 4055, 4130, 4386
TaqII GACCGA 1 cut(s) 1819
TatI WGTACW 2 cut(s) 871, 3118
TauI GCSGC 3 cut(s) 788, 2902, 4065
Tru1I TTAA 9 cut(s) 366, 1193, 1643, 2373, 3195, 3579, 3648, 4116, 4407
Tru9I TTAA 9 cut(s) 366, 1193, 1643, 2373, 3195, 3579, 3648, 4116, 4407
TscAI CASTG 5 cut(s) 1171, 2116, 2984, 4051, 4240
TseFI GTSAC 5 cut(s) 126, 448, 1513, 1793, 2810
Tsp45I GTSAC 5 cut(s) 126, 448, 1513, 1793, 2810
TspGWI ACGGA 3 cut(s) 1409, 3491, 3622
TspRI CASTG 5 cut(s) 1171, 2116, 2984, 4051, 4240
Van91I CCANNNNNTGG 1 cut(s) 797
VneI GTGCAC 1 cut(s) 3560
VpaK11BI GGWCC 4 cut(s) 852, 1682, 3299, 3808
XagI CCTNNNNNAGG 2 cut(s) 3450, 3846
XapI RAATTY 5 cut(s) 534, 1434, 2161, 2893, 3479
XbaI TCTAGA 2 cut(s) 3553, 3794
XceI RCATGY 4 cut(s) 197, 1058, 1888, 3343
XcmI CCANNNNNNNNNTGG 1 cut(s) 814
XmaJI CCTAGG 1 cut(s) 4399
XmiI GTMKAC 3 cut(s) 509, 1247, 2953
XmnI GAANNNNTTC 2 cut(s) 886, 3999
Zsp2I ATGCAT 1 cut(s) 2406
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.