Rh2AG668500

GYF domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0003475
Physical Location & Seq
Reverse (-)
88292527 .. 88300349
7823 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG668500.1

Sequence Viewer

Length: 4443 bp
ATGATGGCTCACGGAAAAGTCAGTCTCCCTCAAGATCTCCTCCCCTCGCCGATCTTCTCCGCCACCAAAGATGGAGCCTTGGAAGGAAATGCTGAGGGAAAGGCTCTGACGGGGTCACTTGATGAGTCAAAAGATCATTTAGCATCTGAGAGCAGCATACCTTTGTCACCACAATGGCTACATGCTAAATCAGTTGAGGCTAAGCCACTTGCAAGTGGAACATCAGGAGAAATCCATGCACCAGGGTCTTTGTCTCATGGTAACTCTATTGATTCCAATGCGAGAGACATTTGGCGTTCAGATGGTTCTCGGGACAGAAAAGATTGGAGGAGGAATGCACCTGATTTAGATGTTAACCGTCGCTGGCGTGAGGAGGAGAGAGAAACAGGCTTACTTGGTAGGAGGGATCGCAAAAAAGAAGATCGCCGTGTAGGAGTCACTTCAACTAGGGATATTACTGAGAATCGAGCAGAAGATCATCGAGCAGGTGGCCCTTCTACTAGGGATGTTAGTGAGAATAAAATTTTGTCTTCTGATCGCTGGCATGAATCCCGGAGAGACAACAAATGGTCATCAAGGTGGGGCCCTGAAGATAAAGACAAGGACTCTCGAATAGAGAAGACAGAACTTGAAAGAGAAGATGCTCATGTTGACAAGCAGTCTCTTGTCAGTAGCAATCGTGCAATTCCTGAACGTGATGGTGATTCTCGCGATAAATGGAGGCCACGCCATCGCATGGAGGTTCAGTCCGGGGGGCCGGCCCCATACCGTGCCGCACCGGGATTTGGGATGGCCAGAGGACAGGTGGAAAAAGTAGGATTTGCTTCTGGACGAGGGAGGTCCAAGACTAATGGTAGCCTACAGATTGGAAAACCTTCCTCTGTATCCACTATCGGGTCTGTTCTGGATCAGAATCAAAGTGTATTGGGAAAAGCTGGCCCCTTTAATGAGAAGTATCGTTACCCCAGGGGAAAGCTCCTTGATATCTATCGTAAGCAAAAGATAGAATCAACTTTTGATATTGTTCCTGATGGGATGGAGCATGTCTCTCCAATTACCCAAGTGGGCTCTATTGAGCCATTGGCCTTTTCTGCACCTCATGCAGAAGAAGAGGCTGTTATGGAAGATATATGGAAGGGAAGAATCACTAGCAGTGAAATGTTGTGCAGTTCCTCCAGAGAAAAAAATGTGTTAAATGATGATGGAACTAGTAATGCCACCTTAAGAAAGGAAGATCAAAAGATTTCACTGCAGACTGAAGAAAATGCCCTATCTACTGGAGAGCCCATCTTAAACAATTCTTTTCCAGTTATTGGTACTGAGGTCTCAACTATCTGTGGCCCACAGACACATATTTTAAATGAATGGGTTGAAGGTGAGCGCAGAGTTCCTATTGTCACAGCAGTCGCTGATGCATCAAATCTGGTTGCTTCAAAGAACTATGATTGCAAAAGTGCTGGGGAGATTGATGGCCTCAGTAATAGTGTCGCTGAACTAAGAATTTCTGGATGCCAGGATGTGAGAGATTTTGGTCTTCCAAAGCATTCTATGTCGGAAAATTTTGAACCAGCTACTACTGTGATTGGTAGCCAGCTTCCTGATGATTCAAGTTCACTTTTTCACATTCCATCTCTACAGCAAACCTCGAGTAGTGATCAATATGTCAAGAGCAACGAGAAGGCAGATCTGGAAAGGGTTGTCTCATTTGAGGATTTAAGTTTATGCTATCTTGATCCTCAAGGAAACATCCAGGGACCTTTCCTGGGGATCGACATAATTTCATGGTTTGATCAAGGATTCTTTGGAACTGACTTACTGGTGCGCTTGGCTGATGCCCCTAATGGTTCTCCTTTTCAAGAACTCGGTGAAATGATGCCAAATCTGAACACTAAATCTGGATTGGTCTCCAACTCCAATTTGCCTACCAACGTGGAATCATTTGGTGGTGTTGGAAGCATCCTGGAAGAGAGAAAAACTGCTCCTCTCTATGAGGAGTCTAACATTCTAAATATCCAACACTGGACTTCATCTGGACTTGAACCTACCTTGAGTGATGATTTGCACTCGAGAATACCTAATAATGGTTACAACTCTGAACTCCAGTACCTTGATAATCAAAGGTTTCAAAATTACGTTGAAGAAGATGAGGAAATAGTGTTTCCCGGAAGGCCTAAAAGTAGCAGTGATTTTCCTCTGAGGAGACCTTCAGCTGATATTCAGGGCTCATTTTCAAATTCCCCAAGCCTTCCTTCCCTTTCAAATGAAGTTTCCGAAAGTGACCTGCCAAATCGCCAGGATGATAAGTTGCACCCTTTTGGCCTGTTGATGTCTGAGCTCAGAGGCAGCTCTCATTTAAGACCTGCTCAAGCTTCCAATGTGTCTTTAGGCATGGATGATCAGGCTCAGTTTAGAGATACCTTGTTTGAGGGAGGTATCTCCAAACTGAATTGGTTGAATAATCCTAATATCCCAATGCATCAAGTAGTGCCTGATATGGAACATCTAATAGAACTACAGCGTCAAGAGCAGCAGCATCACCTATTGGAGCTTCAGCAACAACGCCAGTTTGAGTTTCATCGTCAGCAGCAGCATGAGTTGGAATTTCAACAGCAGCGGCAATTGGAACTCCAGCAACAGCGCCAGTTGGAGCTACAGCAGCAGCGCCAGTTGGAACTTCACCACTTGGAGCTTCAGCAGCAGCACCAGTTGGAGCTACAGCTGCAGCGCCAGTTGGAGCTACAGCAACAGCGCCAGTTTGAGCTACAGCAACAGCGCCAGTTGGAGCTACAGCAACAACGCCAGTTGGAGCTTCAGCAACAGCAGCAACACCATTTGGAGCTTCAACAACAGCAGCAGCGGAAGCTGGAGCTTCAGCAGCTGCAGCGTCATCAGCAACAGCGGCAGTTGGAGTTTCAGCAGCAGCGCCAATTGGAGCTTCAGCAACAGCAGCATCAATTGGAGCTTCCGCCTCAGCACCACTTGCAGCAGCACCTTCGCCATCACCAAAAATTGCAGCAACAACAGCAGTCCCAAGCCGAACAATTGCTCCTCGAACAATTATTGAATCGACAAATGTCTGATGGTTATGGGCAGTGGAAGATGGATGCTACACGAGCCAATCTTCATGATCAGTTACATTCCAGTAACCACCTTCCAAATGATTTGCACCATGTATCTCATTCATCAAGGCACGATCCCTCGCTAGAGCAGATCATCCAAGCAACTATGGGCGAGGGTGCACTCCAAGGACAGACTGATTTTTTTGACCTCATATCGCAGGCCAAGCAAGGGAATAGGAACACTTCAGAGCTGCCACTTCGTCTTAATGAACAAGAGTTGCAAGCACGGCAATTATCTTTGGCATTGAGAGAGCAGCAGCAGATAGAAGGGGAAAGGCGTATTGGTGGGGGTCCTTTTTTGAGGGATCCCGTTGGGCATCACCAGGCTCACATGTTGGGGCATAATTCTTCAGAAAATTACCAGCAACAGCAGAGGTTTCCATCCAATGAACAGGAACCAAGCCATCACGATTGGAGTCATGCCTCTAAGGCTCAGGGAGGGTTTTATGAATCTAACTCTATGGAATTTGATAGATTCTCTGTTCCTGCTGGTTCTCATGGAATGAACTTGGATATTGCAAACATACGTGGACAAGATCTAGATGTGAGGGAACAATATCGGCTTAATTCCTCTATTGATGAGCGTGGATCTCTATCATCTGGCGTACCCTCTCACCGTCGTTTGGTTTCTGATGAGTTTTATGCTTCTCATCCAGTGACTTTTGAAAGCTTGCCATCTGGGAACAATGGGCAGCTAGAAAGTAGCTGCACTGAAGCAGTAATGCCTTATTTGCATCTTGATGCTGAGCAAAAACGACGAGACACGGAAGACACCATTGCCTTTGCTGCTTCAAATAGATTGGCTTCAACTAACGGTGATAAGGAACACTCCAAACAAATTTTGATGGACCTTCATCAAAAACTTGGTCATCAGCCTACACAGTCATCAGAAATTGATTATCAGCATCACCTATCCTCATCCAGAAGTCAGGGAGGCTCTGTACATCTTCCTTTAAATCTTCTTCCAGATCAGTCAGTTGGTATGAGCAACTGCATCACAGAAGGCCCACATAGTTCAAATTTTAGTCTCTTGTTGCAAGATCAGTTGGGTAGTTATGGTATGAATGAGCAACCAAGTAATCTGGCAAGAAGATCTGATTCCAGAGCATTTATGGACGCTCAATTCTTGTCAGGAACCAGGGATGCTCCTCACGAAGGCAACATGGCAAACCAACAAGACACAGCCATTGAGAGCGGGGAACTACCTAGTAATACCCATAGCAGGCATAGCTCACTGAGCAGTGCTGGTGGATGTGGGGGTTTTCACAGCTCTGAGATGGGATTAGATAAATCACTTGGAGAAGATGTTTCTGATGGGAGGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
GYF

Protein Analysis

1480

Amino Acids

166.82

Weight (kDa)

5.49

Isoelectric Point (pI)

63.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GYF PF02213 576 - 620 1.3e-10 GYF domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 476
Acc36I ACCTGC 3 cut(s) 476, 2289, 2368
AccB7I CCANNNNNTGG 2 cut(s) 736, 1313
AccBSI CCGCTC 1 cut(s) 4314
AccII CGCG 1 cut(s) 711
AciI CCGC 7 cut(s) 60, 774, 2614, 2857, 2899, 2966, 4314
AclWI GGATC 8 cut(s) 414, 915, 1727, 1775, 3188, 3419, 3432, 3715
AcoI YGGCCR 1 cut(s) 792
AcsI RAATTY 8 cut(s) 522, 1500, 1558, 2233, 2600, 3584, 3957, 4138
AfaI GTAC 4 cut(s) 1318, 2105, 3726, 4062
AflII CTTAAG 1 cut(s) 1222
AflIII ACRYGT 1 cut(s) 3450
AhdI GACNNNNNGTC 1 cut(s) 658
AhlI ACTAGT 1 cut(s) 1208
AjnI CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3441, 4256
AjuI GAANNNNNNNTTGG 6 cut(s) 2233, 2265, 2919, 2951, 3384, 3416
AloI GAACNNNNNNTCC 2 cut(s) 3030, 3062
Alw21I GWGCWC 2 cut(s) 2337, 3241
Alw44I GTGCAC 1 cut(s) 3237
AlwI GGATC 8 cut(s) 414, 915, 1727, 1775, 3188, 3419, 3432, 3715
AlwNI CAGNNNCTG 2 cut(s) 1409, 2878
Ama87I CYCGRG 3 cut(s) 309, 1645, 2065
ApaI GGGCCC 1 cut(s) 587
ApaLI GTGCAC 1 cut(s) 3237
ApoI RAATTY 8 cut(s) 522, 1500, 1558, 2233, 2600, 3584, 3957, 4138
ArsI GACNNNNNNTTYG 2 cut(s) 3970, 4002
Asp700I GAANNNNTTC 1 cut(s) 874
AspLEI GCGC 8 cut(s) 1383, 1824, 2640, 2664, 2727, 2751, 2775, 2925
AsuC2I CCSGG 4 cut(s) 553, 751, 780, 2163
AvaI CYCGRG 3 cut(s) 309, 1645, 2065
AvaII GGWCC 4 cut(s) 840, 1754, 3410, 3967
BaeGI GKGCMC 2 cut(s) 587, 3241
BaeI ACNNNNGTAYC 4 cut(s) 1308, 1341, 2087, 2120
BalI TGGCCA 1 cut(s) 794
BamHI GGATCC 1 cut(s) 3424
BanII GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
BarI GAAGNNNNNNTAC 2 cut(s) 944, 976
BauI CACGAG 1 cut(s) 3111
BbsI GAAGAC 4 cut(s) 522, 626, 1526, 3894
Bbv12I GWGCWC 2 cut(s) 2337, 3241
BbvCI CCTCAGC 2 cut(s) 93, 2970
BceAI ACGGC 2 cut(s) 411, 3362
BcgI CGANNNNNNTGC 2 cut(s) 2975, 3009
BciT130I CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3443, 4258
BciVI GTATCC 1 cut(s) 895
BclI TGATCA 4 cut(s) 1654, 1789, 2395, 3127
BcnI CCSGG 4 cut(s) 553, 751, 780, 2163
BcuI ACTAGT 1 cut(s) 1208
BfaI CTAG 8 cut(s) 447, 501, 1149, 1209, 3203, 3659, 3815, 4326
BfoI RGCGCY 6 cut(s) 2641, 2665, 2728, 2752, 2776, 2926
BfrI CTTAAG 1 cut(s) 1222
BfuAI ACCTGC 3 cut(s) 476, 2289, 2368
BfuI GTATCC 1 cut(s) 895
BglI GCCNNNNNGGC 1 cut(s) 3548
BglII AGATCT 4 cut(s) 34, 1684, 3655, 4211
BlpI GCTNAGC 2 cut(s) 201, 3864
Bme18I GGWCC 4 cut(s) 840, 1754, 3410, 3967
BmeRI GACNNNNNGTC 1 cut(s) 658
BmeT110I CYCGRG 3 cut(s) 309, 1645, 2065
BoxI GACNNNNGTC 1 cut(s) 3073
BpiI GAAGAC 4 cut(s) 522, 626, 1526, 3894
BplI GAGNNNNNCTC 2 cut(s) 1031, 1063
BpmI CTGGAG 5 cut(s) 1159, 1299, 2084, 2612, 2885
Bpu10I CCTNAGC 3 cut(s) 93, 2970, 3552
Bpu1102I GCTNAGC 2 cut(s) 201, 3864
BpuEI CTTGAG 4 cut(s) 15, 1722, 2068, 2349
BpuMI CCSGG 4 cut(s) 553, 751, 780, 2163
BsaAI YACGTR 1 cut(s) 3647
BsaBI GATNNNNATC 3 cut(s) 912, 987, 3712
BsaI GGTCTC 3 cut(s) 1330, 1909, 2194
BsaJI CCNNGG 9 cut(s) 78, 242, 750, 965, 966, 1750, 1762, 3244, 4257
BsaXI ACNNNNNCTCC 8 cut(s) 731, 761, 3030, 3060, 4044, 4074, 4410, 4440
Bse118I RCCGGY 1 cut(s) 757
Bse3DI GCAATG 1 cut(s) 3894
Bse8I GATNNNNATC 3 cut(s) 912, 987, 3712
BseBI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3443, 4258
BseDI CCNNGG 9 cut(s) 78, 242, 750, 965, 966, 1750, 1762, 3244, 4257
BseJI GATNNNNATC 3 cut(s) 912, 987, 3712
BseMI GCAATG 1 cut(s) 3894
BseRI GAGGAG 9 cut(s) 29, 343, 386, 389, 1971, 2006, 2212, 3038, 4257
BseSI GKGCMC 2 cut(s) 587, 3241
BseYI CCCAGC 1 cut(s) 1457
BsgI GTGCAG 3 cut(s) 1077, 1186, 3811
Bsh1236I CGCG 1 cut(s) 711
BsiHKAI GWGCWC 2 cut(s) 2337, 3241
BsiHKCI CYCGRG 3 cut(s) 309, 1645, 2065
BsiSI CCGG 5 cut(s) 553, 750, 758, 779, 2163
BslFI GGGAC 3 cut(s) 326, 1767, 3013
BsmFI GGGAC 3 cut(s) 326, 1767, 3013
BsmI GAATGC 2 cut(s) 340, 1543
Bso31I GGTCTC 3 cut(s) 1330, 1909, 2194
BsoBI CYCGRG 3 cut(s) 309, 1645, 2065
Bsp120I GGGCCC 1 cut(s) 583
Bsp1286I GDGCHC 6 cut(s) 587, 1070, 1287, 2225, 2337, 3241
Bsp1407I TGTACA 1 cut(s) 4060
Bsp1720I GCTNAGC 2 cut(s) 201, 3864
Bsp68I TCGCGA 1 cut(s) 711
BspACI CCGC 7 cut(s) 60, 774, 2614, 2857, 2899, 2966, 4314
BspFNI CGCG 1 cut(s) 711
BspHI TCATGA 1 cut(s) 3124
BspMAI CTGCAG 3 cut(s) 1254, 2724, 2883
BspMI ACCTGC 3 cut(s) 476, 2289, 2368
BspPI GGATC 8 cut(s) 414, 915, 1727, 1775, 3188, 3419, 3432, 3715
BspTI CTTAAG 1 cut(s) 1222
BspTNI GGTCTC 3 cut(s) 1330, 1909, 2194
BsrBI CCGCTC 1 cut(s) 4314
BsrDI GCAATG 1 cut(s) 3894
BsrFI RCCGGY 1 cut(s) 757
BsrGI TGTACA 1 cut(s) 4060
BssAI RCCGGY 1 cut(s) 757
BssECI CCNNGG 9 cut(s) 78, 242, 750, 965, 966, 1750, 1762, 3244, 4257
BssSI CACGAG 1 cut(s) 3111
BssT1I CCWWGG 2 cut(s) 78, 3244
Bst2BI CACGAG 1 cut(s) 3111
Bst2UI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3443, 4258
Bst4CI ACNGT 6 cut(s) 359, 770, 1579, 3737, 3935, 4002
Bst6I CTCTTC 2 cut(s) 1104, 1959
BstAFI CTTAAG 1 cut(s) 1222
BstAPI GCANNNNNTGC 2 cut(s) 1100, 2980
BstAUI TGTACA 1 cut(s) 4060
BstBAI YACGTR 1 cut(s) 3647
BstC8I GCNNGC 9 cut(s) 365, 542, 759, 937, 1592, 3279, 3342, 3791, 4343
BstENI CCTNNNNNAGG 1 cut(s) 4272
BstFNI CGCG 1 cut(s) 711
BstH2I RGCGCY 6 cut(s) 2641, 2665, 2728, 2752, 2776, 2926
BstHHI GCGC 8 cut(s) 1383, 1824, 2640, 2664, 2727, 2751, 2775, 2925
BstNI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3443, 4258
BstNSI RCATGY 3 cut(s) 185, 1046, 3454
BstPAI GACNNNNGTC 1 cut(s) 3073
BstSLI GKGCMC 2 cut(s) 587, 3241
BstUI CGCG 1 cut(s) 711
BstV2I GAAGAC 4 cut(s) 522, 626, 1526, 3894
BstX2I RGATCY 6 cut(s) 34, 1684, 3424, 3655, 3707, 4211
BstYI RGATCY 6 cut(s) 34, 1684, 3424, 3655, 3707, 4211
BsuI GTATCC 1 cut(s) 895
BtgZI GCGATG 1 cut(s) 716
BtsI GCAGTG 5 cut(s) 1159, 1247, 2188, 3098, 4366
BtsIMutI CAGTG 9 cut(s) 1159, 1247, 2017, 2188, 3098, 3780, 3828, 4352, 4366
BtuMI TCGCGA 1 cut(s) 711
BveI ACCTGC 3 cut(s) 476, 2289, 2368
Cac8I GCNNGC 9 cut(s) 365, 542, 759, 937, 1592, 3279, 3342, 3791, 4343
CaiI CAGNNNCTG 2 cut(s) 1409, 2878
CciI TCATGA 1 cut(s) 3124
CfoI GCGC 8 cut(s) 1383, 1824, 2640, 2664, 2727, 2751, 2775, 2925
Cfr10I RCCGGY 1 cut(s) 757
CseI GACGC 3 cut(s) 2507, 2873, 4244
Csp6I GTAC 4 cut(s) 1317, 2104, 3725, 4061
CviQI GTAC 4 cut(s) 1317, 2104, 3725, 4061
DraI TTTAAA 2 cut(s) 1359, 4074
DriI GACNNNNNGTC 1 cut(s) 658
EaeI YGGCCR 1 cut(s) 792
Eam1104I CTCTTC 2 cut(s) 1104, 1959
Eam1105I GACNNNNNGTC 1 cut(s) 658
EarI CTCTTC 2 cut(s) 1104, 1959
EciI GGCGGA 2 cut(s) 49, 2955
Ecl136II GAGCTC 1 cut(s) 2335
Eco130I CCWWGG 2 cut(s) 78, 3244
Eco147I AGGCCT 1 cut(s) 2170
Eco24I GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
Eco31I GGTCTC 3 cut(s) 1330, 1909, 2194
Eco32I GATATC 1 cut(s) 985
Eco47I GGWCC 4 cut(s) 840, 1754, 3410, 3967
Eco53kI GAGCTC 1 cut(s) 2335
Eco88I CYCGRG 3 cut(s) 309, 1645, 2065
EcoICRI GAGCTC 1 cut(s) 2335
EcoNI CCTNNNNNAGG 1 cut(s) 4272
EcoO109I RGGNCCY 4 cut(s) 583, 584, 1754, 3410
EcoRII CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3441, 4256
EcoRV GATATC 1 cut(s) 985
EcoT14I CCWWGG 2 cut(s) 78, 3244
EcoT22I ATGCAT 2 cut(s) 1417, 2478
EcoT38I GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
ErhI CCWWGG 2 cut(s) 78, 3244
FalI AAGNNNNNCTT 2 cut(s) 2233, 2265
FaqI GGGAC 3 cut(s) 326, 1767, 3013
FauI CCCGC 1 cut(s) 4307
FbaI TGATCA 4 cut(s) 1654, 1789, 2395, 3127
FriOI GRGCYC 5 cut(s) 587, 1070, 1287, 2225, 2337
FseI GGCCGGCC 1 cut(s) 761
FspBI CTAG 8 cut(s) 447, 501, 1149, 1209, 3203, 3659, 3815, 4326
GlaI GCGC 8 cut(s) 1382, 1823, 2639, 2663, 2726, 2750, 2774, 2924
GsaI CCCAGC 1 cut(s) 1461
GsuI CTGGAG 5 cut(s) 1159, 1299, 2084, 2612, 2885
HaeII RGCGCY 6 cut(s) 2641, 2665, 2728, 2752, 2776, 2926
HapII CCGG 5 cut(s) 553, 750, 758, 779, 2163
HgaI GACGC 3 cut(s) 2507, 2873, 4244
HhaI GCGC 8 cut(s) 1383, 1824, 2640, 2664, 2727, 2751, 2775, 2925
Hin6I GCGC 8 cut(s) 1381, 1822, 2638, 2662, 2725, 2749, 2773, 2923
HinP1I GCGC 8 cut(s) 1381, 1822, 2638, 2662, 2725, 2749, 2773, 2923
HincII GTYRAC 2 cut(s) 355, 652
HindII GTYRAC 2 cut(s) 355, 652
HindIII AAGCTT 2 cut(s) 2367, 3787
HpaI GTTAAC 1 cut(s) 355
HpaII CCGG 5 cut(s) 553, 750, 758, 779, 2163
Hpy166II GTNNAC 5 cut(s) 355, 652, 1613, 3239, 3650
Hpy8I GTNNAC 5 cut(s) 355, 652, 1613, 3239, 3650
Hpy99I CGWCG 3 cut(s) 363, 3741, 3879
HpyCH4III ACNGT 6 cut(s) 359, 770, 1579, 3737, 3935, 4002
HpyCH4IV ACGT 4 cut(s) 694, 1929, 2133, 3646
HpySE526I ACGT 4 cut(s) 694, 1929, 2133, 3646
HspAI GCGC 8 cut(s) 1381, 1822, 2638, 2662, 2725, 2749, 2773, 2923
KroI GCCGGC 1 cut(s) 757
KroNI GCCGGC 1 cut(s) 759
Ksp22I TGATCA 4 cut(s) 1654, 1789, 2395, 3127
KspAI GTTAAC 1 cut(s) 355
MaeI CTAG 8 cut(s) 447, 501, 1149, 1209, 3203, 3659, 3815, 4326
MaeII ACGT 4 cut(s) 694, 1929, 2133, 3646
MbiI CCGCTC 1 cut(s) 4314
MfeI CAATTG 4 cut(s) 2618, 2927, 2954, 3041
MflI RGATCY 6 cut(s) 34, 1684, 3424, 3655, 3707, 4211
MhlI GDGCHC 6 cut(s) 587, 1070, 1287, 2225, 2337, 3241
MlsI TGGCCA 1 cut(s) 794
MluNI TGGCCA 1 cut(s) 794
MlyI GAGTC 5 cut(s) 134, 444, 599, 2003, 3544
Mox20I TGGCCA 1 cut(s) 794
Mph1103I ATGCAT 2 cut(s) 1417, 2478
MroNI GCCGGC 1 cut(s) 757
MroXI GAANNNNTTC 1 cut(s) 874
MscI TGGCCA 1 cut(s) 794
MslI CAYNNNNRTG 3 cut(s) 172, 577, 3858
Msp20I TGGCCA 1 cut(s) 794
MspA1I CMGCKG 6 cut(s) 2210, 2614, 2719, 2857, 2878, 2899
MspCI CTTAAG 1 cut(s) 1222
MspI CCGG 5 cut(s) 553, 750, 758, 779, 2163
MunI CAATTG 4 cut(s) 2618, 2927, 2954, 3041
Mva1269I GAATGC 2 cut(s) 340, 1543
MvaI CCWGG 9 cut(s) 243, 967, 1514, 1751, 1763, 1961, 2294, 3443, 4258
MvnI CGCG 1 cut(s) 711
NaeI GCCGGC 1 cut(s) 759
NciI CCSGG 4 cut(s) 553, 751, 780, 2163
NgoMIV GCCGGC 1 cut(s) 757
NmuCI GTSAC 6 cut(s) 114, 165, 436, 1396, 2276, 3775
NruI TCGCGA 1 cut(s) 711
NsiI ATGCAT 2 cut(s) 1417, 2478
NspI RCATGY 3 cut(s) 185, 1046, 3454
PaeR7I CTCGAG 2 cut(s) 1645, 2065
PagI TCATGA 1 cut(s) 3124
PaqCI CACCTGC 1 cut(s) 476
PasI CCCWGGG 1 cut(s) 966
PceI AGGCCT 1 cut(s) 2170
PciI ACATGT 1 cut(s) 3450
PctI GAATGC 2 cut(s) 340, 1543
PdiI GCCGGC 1 cut(s) 759
PdmI GAANNNNTTC 1 cut(s) 874
PflFI GACNNNGTC 1 cut(s) 112
PflMI CCANNNNNTGG 2 cut(s) 736, 1313
PfoI TCCNGGA 3 cut(s) 551, 1959, 2161
PleI GAGTC 5 cut(s) 133, 443, 599, 2002, 3543
PpsI GAGTC 5 cut(s) 133, 443, 599, 2002, 3543
Ppu21I YACGTR 1 cut(s) 3647
PpuMI RGGWCCY 2 cut(s) 1754, 3410
PscI ACATGT 1 cut(s) 3450
PshAI GACNNNNGTC 1 cut(s) 3073
Psp124BI GAGCTC 1 cut(s) 2337
Psp5II RGGWCCY 2 cut(s) 1754, 3410
Psp6I CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3441, 4256
PspFI CCCAGC 1 cut(s) 1457
PspGI CCWGG 9 cut(s) 241, 965, 1512, 1749, 1761, 1959, 2292, 3441, 4256
PspOMI GGGCCC 1 cut(s) 583
PspPPI RGGWCCY 2 cut(s) 1754, 3410
PspXI VCTCGAGB 1 cut(s) 1645
PstI CTGCAG 3 cut(s) 1254, 2724, 2883
PstNI CAGNNNCTG 2 cut(s) 1409, 2878
PsuI RGATCY 6 cut(s) 34, 1684, 3424, 3655, 3707, 4211
PsyI GACNNNGTC 1 cut(s) 112
PvuII CAGCTG 3 cut(s) 2210, 2719, 2878
RigI GGCCGGCC 1 cut(s) 761
RruI TCGCGA 1 cut(s) 711
RsaI GTAC 4 cut(s) 1318, 2105, 3726, 4062
RsaNI GTAC 4 cut(s) 1317, 2104, 3725, 4061
RseI CAYNNNNRTG 3 cut(s) 172, 577, 3858
SacI GAGCTC 1 cut(s) 2337
SchI GAGTC 5 cut(s) 134, 444, 599, 2003, 3544
SduI GDGCHC 6 cut(s) 587, 1070, 1287, 2225, 2337, 3241
Sfr274I CTCGAG 2 cut(s) 1645, 2065
SinI GGWCC 4 cut(s) 840, 1754, 3410, 3967
SlaI CTCGAG 2 cut(s) 1645, 2065
SmiMI CAYNNNNRTG 3 cut(s) 172, 577, 3858
SmlI CTYRAG 7 cut(s) 30, 1222, 1645, 1737, 2047, 2065, 2364
SmoI CTYRAG 7 cut(s) 30, 1222, 1645, 1737, 2047, 2065, 2364
SpeI ACTAGT 1 cut(s) 1208
SseBI AGGCCT 1 cut(s) 2170
SsiI CCGC 7 cut(s) 60, 774, 2614, 2857, 2899, 2966, 4314
SspMI CTAG 8 cut(s) 447, 501, 1149, 1209, 3203, 3659, 3815, 4326
SstI GAGCTC 1 cut(s) 2337
StuI AGGCCT 1 cut(s) 2170
StyI CCWWGG 2 cut(s) 78, 3244
TaaI ACNGT 6 cut(s) 359, 770, 1579, 3737, 3935, 4002
TaiI ACGT 4 cut(s) 697, 1932, 2136, 3649
TaqI TCGA 8 cut(s) 466, 481, 610, 1646, 1770, 2066, 3051, 3067
TatI WGTACW 1 cut(s) 4060
TauI GCSGC 3 cut(s) 776, 2617, 2902
TscAI CASTG 9 cut(s) 1159, 1254, 2024, 2188, 3098, 3780, 3835, 4359, 4366
TseFI GTSAC 6 cut(s) 114, 165, 436, 1396, 2276, 3775
Tsp45I GTSAC 6 cut(s) 114, 165, 436, 1396, 2276, 3775
TspGWI ACGGA 2 cut(s) 27, 3899
TspRI CASTG 9 cut(s) 1159, 1254, 2024, 2188, 3098, 3780, 3835, 4359, 4366
Tth111I GACNNNGTC 1 cut(s) 112
Van91I CCANNNNNTGG 2 cut(s) 736, 1313
Vha464I CTTAAG 1 cut(s) 1222
VneI GTGCAC 1 cut(s) 3237
VpaK11BI GGWCC 4 cut(s) 840, 1754, 3410, 3967
XagI CCTNNNNNAGG 1 cut(s) 4272
XapI RAATTY 8 cut(s) 522, 1500, 1558, 2233, 2600, 3584, 3957, 4138
XbaI TCTAGA 1 cut(s) 3658
XceI RCATGY 3 cut(s) 185, 1046, 3454
XcmI CCANNNNNNNNNTGG 2 cut(s) 802, 4228
XhoI CTCGAG 2 cut(s) 1645, 2065
XmnI GAANNNNTTC 1 cut(s) 874
XspI CTAG 8 cut(s) 447, 501, 1149, 1209, 3203, 3659, 3815, 4326
Zsp2I ATGCAT 2 cut(s) 1417, 2478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.